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中国科学院昆明植物研究所知识管理系统
Knowledge Management System of Kunming Institute of Botany,CAS
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GST,p < 0.001) and low levels of seed-based gene flow. C. debaoensis (Cycadaceae) is an endangered species restricted to the border of Guangxi and Yunnan province in southwest China. This species has been classified into two types: sand and karst, according to the soil matrix they grow on. We examined chloroplast sequence variation of the cpDNA sequences from 11 populations of this species. Significant population genetic differentiation was detected (GST= 0.684 and FST = 0.74160). There was marked genetic differentiation between populations in the sand and karst regions and no expansion was detected. Climate changes during glacial periods have had significant effects on the current distribution of cycads. The molecular phylogenetic data, together with the geographic distribution of the haplotypes, suggest that C. debaoensis experienced range contraction during glacial periods, and that the current populations are still confined to the original refugia in southwest China which have favorable habitats in glacial period. These results imply that small refugia were maintained in both sand and karst regions during the LGM (last glacial maximum). This species had no postglacial recolonization and only stayed in these refugia up to now. The low within-population diversity of C. debaoensis suggests that there were strong bottleneck events or founder effects within each separate region during the Quaternary climatic oscillations. Relatively high genetic and haplotype diversities were detected in the newly discovered populations, which located at intermediate locality of sand regions and had morphological variation; this is probably the consequence of the admixture of different haplotypes colonizing the area from separate sources. C. micholitzii occurs in the Annan Highlands in central Vietnam near the Laos border. C. bifida occurs in North Vietnam; its distribution extends across the border into adjacent localities in Guangxi and Yunnan in China. For the comparability between them,theywere considered as the same species C. micholitzii by many academicians. The cpDNA sequences from 11 populations showed that these very controversial species, C. micholitzii and C. bifida, is paraphyletic and should belong to the same species C. micholitzii. AMOVA analysis showed that the component of among-population within region/species (76.46%) was unexpectedly larger than the among-species/region component (14.97%), which also indicates that there is no justification for recognizing two species as C. micholitzii and C. bifida. This hypothesis was also supported by the geological data, especially the neotectonic history of the indo-china block, which started to move south since Oligocene and cause the geographic isolation of these two groups. Therefore, the most likely explanation to the phenotypic similarities between these two groups may be the retention of ancestral polymorphisms in the paraphyletic group due to incomplete lineage sorting. Furthermore, the similarities may also be ascribed to pollen-mediated gene flow among geographically proximate populations and/or phenotypic convergence under similar selection schemes in the same region. C.micholitzi had the higest genetic diversity (HT = 0.980,) and genetic differentiation (GST = 0.830, NST = 0.915) among the C. micholitzii complex. The high genetic diversity might be attributed to its long evolutionary history, highly diverse habitats. The ineffective mode of seed dispersal and dramatic neotectonic movement in the distribution range of this species could result in the high genetic differentiation. 2. Phylogeographic analysis based on nuclear ribosomal sequences, We sequenced the nrDNA ITS in all 27 populations sampled, 7 haplotypes were identified, among which C. micholitzii had 6, while C. multipinnata, C. longipetiolula and C. debaoensis shared the remaining one. Compared to chloroplast genes, nuclear genes had higher correlation between genetic and geographical distance, but lower interspecies differentiation (54.42% vs 25.24%). Phylogeographical structure of C. micholitzii and C.bifida based on ITS Variation was consistent with the morphology differentiation. This similar in nuclear gene should be ascribed to pollen-mediated gene flow among geographically proximate populations.Long-distance gene flow over the two groups was clearly interrupted, which brought on the nrDNA genetic differenciation between the geographically isolated groups, to a certain extent affected the morphological variation. 3. Interspecies relationships among Cycas micholitzii complex, We analysed chloroplast sequence variation of the atpB-rbcL and psbA-trnH intergenic spacers in 27 populations sampled of C. micholitzii complex, AMOVA analysis showed that the component of among-species/region component (59.21%). However, phylogenic analysis showed that the haplotypes of C. micholitzii complex couldn`t grouped into four clusters closely corresponding to the narrowly defined C. micholitzi, C. multipinnata, C. debaoensis and C. longipetiolula. We concluded that the conflict may result from several factors: firstly incomplete lineage sorting of C. micholitzii; secondly hybridization/introgression of sympatrically cycads, which would be supported by evidence base on nrDNA ITS sequences; thirdly intramolecular recombination in cpDNA of cycads; eventually the neotectonic movement in the distribution range of this species.","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&sort_by=2&isNonaffiliated=false&search_type=-1&query1=Tibetan&order=desc&&fq=dc.project.title_filter%3ACycas%5C+micholitzii%5C+complex%5C+is%5C+composed%5C+of%5C+5%5C+species%5C%3A%5C+C.%5C+micholitzii%5C+Dyer%2C%5C+C.%5C+bifida%5C+%5C%28Dyer%5C%29%5C+K.%5C+D.%5C+Hill%2CC.%5C+longipetiolula%5C+D.%5C+Y.%5C+Wang%2C%5C+C.%5C+debaoensis%5C+Y.%5C+C.%5C+Zhong%5C+et%5C+C%5C+J.%5C+Chen%2C%5C+C.%5C+multipinnata%5C+C%5C+J.%5C+Chen%5C+et%5C+S.%5C+Y.%5C+Yang%EF%BC%8Cand%5C+distributed%5C+from%5C+southwest%5C+China%5C+to%5C+central%5C+Vietnam%5C+and%5C+eastern%5C+Laos.%5C+Based%5C+on%5C+sequence%5C+data%5C+from%5C+two%5C+maternally%5C+inherited%5C+cpDNA%5C+and%5C+one%5C+biparentally%5C+nuclear%5C+DNA%5C+fragments%2C%5C+our%5C+study%5C+revealed%5C+the%5C+population%5C+genetic%5C+structure%5C+of%5C+C.%5C+micholitzii%5C+complex%5C+and%5C+explored%5C+the%5C+potential%5C+causes.%5C+The%5C+evolutionary%5C+and%5C+demographic%5C+histories%5C+were%5C+investigated.%5C+The%5C+genetic%5C+relationship%5C+among%5C+species%5C+in%5C+the%5C+complex%5C+was%5C+also%5C+clarified.The%5C+results%5C+were%5C+summarized%5C+as%5C+follows%5C%3A%5C+1.%5C+Phylogeographic%5C+analysis%5C+based%5C+on%5C+chloroplast%5C+sequences%EF%BC%8CWe%5C+examined%5C+chloroplast%5C+sequence%5C+variation%5C+of%5C+the%5C+atpB%5C-rbcLand%5C+psbA%5C-trnHintergenic%5C+spacers%5C+in%5C+27%5C+populations%5C+of%5C+C.%5C+micholitzii%5C+complex%2C%5C+recovering%5C+26%5C+haplotypes.%5C+The%5C+average%5C+within%5C-population%5C+diversity%5C+%5C%28HS%5C+%3D%5C+0.140%5C%29%5C+was%5C+low%5C+while%5C+total%5C+diversity%5C+%5C%28HT%5C+%3D%5C+0.911%5C%29%5C+was%5C+high.%5C+Population%5C+differentiation%5C+was%5C+also%5C+high%5C%28GST%5C+%3D%5C+0.846%2C%5C+NST%5C+%3D%5C+0.919%5C%29%2C%5C+indicating%5C+significant%5C+phylogeographical%5C+structure%5C+%5C%28NST%5C+%3E%5C+GST%2Cp%5C+%3C%5C+0.001%5C%29%5C+and%5C+low%5C+levels%5C+of%5C+seed%5C-based%5C+gene%5C+flow.%5C+C.%5C+debaoensis%5C+%5C%28Cycadaceae%5C%29%5C+is%5C+an%5C+endangered%5C+species%5C+restricted%5C+to%5C+the%5C+border%5C+of%5C+Guangxi%5C+and%5C+Yunnan%5C+province%5C+in%5C+southwest%5C+China.%5C+This%5C+species%5C+has%5C+been%5C+classified%5C+into%5C+two%5C+types%5C%3A%5C+sand%5C+and%5C+karst%2C%5C+according%5C+to%5C+the%5C+soil%5C+matrix%5C+they%5C+grow%5C+on.%5C+We%5C+examined%5C+chloroplast%5C+sequence%5C+variation%5C+of%5C+the%5C+cpDNA%5C+sequences%5C+from%5C+11%5C+populations%5C+of%5C+this%5C+species.%5C+Significant%5C+population%5C+genetic%5C+differentiation%5C+was%5C+detected%5C+%5C%28GST%3D%5C+0.684%5C+and%5C+FST%5C+%3D%5C+0.74160%5C%29.%5C+There%5C+was%5C+marked%5C+genetic%5C+differentiation%5C+between%5C+populations%5C+in%5C+the%5C+sand%5C+and%5C+karst%5C+regions%5C+and%5C+no%5C+expansion%5C+was%5C+detected.%5C+Climate%5C+changes%5C+during%5C+glacial%5C+periods%5C+have%5C+had%5C+significant%5C+effects%5C+on%5C+the%5C+current%5C+distribution%5C+of%5C+cycads.%5C+The%5C+molecular%5C+phylogenetic%5C+data%2C%5C+together%5C+with%5C+the%5C+geographic%5C+distribution%5C+of%5C+the%5C+haplotypes%2C%5C+suggest%5C+that%5C+C.%5C+debaoensis%5C+experienced%5C+range%5C+contraction%5C+during%5C+glacial%5C+periods%2C%5C+and%5C+that%5C+the%5C+current%5C+populations%5C+are%5C+still%5C+confined%5C+to%5C+the%5C+original%5C+refugia%5C+in%5C+southwest%5C+China%5C+which%5C+have%5C+favorable%5C+habitats%5C+in%5C+glacial%5C+period.%5C+These%5C+results%5C+imply%5C+that%5C+small%5C+refugia%5C+were%5C+maintained%5C+in%5C+both%5C+sand%5C+and%5C+karst%5C+regions%5C+during%5C+the%5C+LGM%5C+%5C%28last%5C+glacial%5C+maximum%5C%29.%5C+This%5C+species%5C+had%5C+no%5C+postglacial%5C+recolonization%5C+and%5C+only%5C+stayed%5C+in%5C+these%5C+refugia%5C+up%5C+to%5C+now.%5C+The%5C+low%5C+within%5C-population%5C+diversity%5C+of%5C+C.%5C+debaoensis%5C+suggests%5C+that%5C+there%5C+were%5C+strong%5C+bottleneck%5C+events%5C+or%5C+founder%5C+effects%5C+within%5C+each%5C+separate%5C+region%5C+during%5C+the%5C+Quaternary%5C+climatic%5C+oscillations.%5C+Relatively%5C+high%5C+genetic%5C+and%5C+haplotype%5C+diversities%5C+were%5C+detected%5C+in%5C+the%5C+newly%5C+discovered%5C+populations%2C%5C+which%5C+located%5C+at%5C+intermediate%5C+locality%5C+of%5C+sand%5C+regions%5C+and%5C+had%5C+morphological%5C+variation%5C%3B%5C+this%5C+is%5C+probably%5C+the%5C+consequence%5C+of%5C+the%5C+admixture%5C+of%5C+different%5C+haplotypes%5C+colonizing%5C+the%5C+area%5C+from%5C+separate%5C+sources.%5C+%5C+C.%5C+micholitzii%5C+occurs%5C+in%5C+the%5C+Annan%5C+Highlands%5C+in%5C+central%5C+Vietnam%5C+near%5C+the%5C+Laos%5C+border.%5C+C.%5C+bifida%5C+occurs%5C+in%5C+North%5C+Vietnam%5C%3B%5C+its%5C+distribution%5C+extends%5C+across%5C+the%5C+border%5C+into%5C+adjacent%5C+localities%5C+in%5C+Guangxi%5C+and%5C+Yunnan%5C+in%5C+China.%5C+For%5C+the%5C+comparability%5C+between%5C+them%2Ctheywere%5C+considered%5C+as%5C+the%5C+same%5C+species%5C+C.%5C+micholitzii%5C+by%5C+many%5C+academicians.%5C+T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Plant RD Center[Y65J8322C1]","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&sort_by=2&isNonaffiliated=false&search_type=-1&query1=Tibetan&order=desc&&fq=dc.project.title_filter%3ADr.%5C+Plant%5C+RD%5C+Center%5C%5BY65J8322C1%5C%5D"},{"jsname":"During a field trip at a brule in Shangri-La, a mixed population of Ligularia Cass. was found, which including L. subspicata (Bur. et Franch.) Hand.-Mazz., L. nelumbifolia (Bur. et Franch.) Hand.-Mazz., L. tongolensis (Franch.) Hand.-Mazz., L. cymbulifera (W.W.Smith) Hand.-Mazz., L. lingiana S.W.Liu, and also some individuals morphologically intermediate between L. subspicata and L. nelumbifolia. Hence, these intermediate individuals were preliminarily assumed as natural hybrids of the two Ligularia. According to their morphology, they’re assumed to form hybrids A and B. Through careful comparison of specimens in herbarium and those we collected, the inflorescence of putative hybrid A is close to L. nelumbifolia, but the shape of laminae are intergradation of L. subspicata and L. nelumbifolia; overall morphology of putative hybrids B is similar to L. nelumbifolia, but inflorescence color is as same as L. subspicata. Compared to L. nelumbifolia (39%) and L. subspicata (36.8%), the germination rate of putative hybrid B (45.7%) slightly higher than the two; but that of hybrid A is extraordinarily low (0.3%). One possible interpretation of the low rate is hybridization. 60 individuals were collected, including putative parents, other 4 species of Ligularia nearby, putative hybrid A and B. They were all direct sequenced of four cpDNA fragments, and direct sequenced or cloning sequenced of nrDNA ITS4-5. The results support that L. nelumbifolia and L. subspicata are parents of putative hybrid A, and the majority female parent is L. subspicata, L. vellerea may also be involved in the hybridization in some degree; the nuclear sequences of putative hybrid B have no superposition, and its chloroplast DNA sequences are identical with L. nelumbifolia, so putative hybrid B could not be hybrid; and there are backcross individuals exist among the putative parent L. subspicata. NewHybrids analysis of ISSR markers indicated that, the individuals of putative hybrid A are almost L. nelumbifolia and L. subspicata F1 hybrid generation (10/11), only 1/11 possibly backcross or other forms; all individuals of hybrid B are L. nelumbifolia; except one individual of L. subspicata as backcrossed, the other parent individuals are 100% reliable. This study focused on molecular evidence, complemented by ecological, reproductive and other characteristics, we demonstrated that the morphologically intermediate individuals’ origin, and the probability of belonging to each parental or hybrid class. And concluded that L. nelumbifolia and L. subspicata are the parents of putative hybrid A, L. vellerea may also be involved in the hybridization in some degree, hybrids mainly are the first generation, a few individuals may be involved in backcross, and most probably backcross with L. subspicata according to the anthesis, while the assumption of hybrid B is not 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The Forest Filter Effect vs. Cold Trapping Effect on the AltitudinalDistribution of PCBs: A Case Study of Mt. Gongga, Eastern Tibetan Plateau
期刊论文
出版物, 3111, 期号: 0, 页码: 1-32
Authors:
Xin Liu
;
Jun Li
;
Qian Zheng
;
Haijian Bing
;
Ruijie Zhang
;
Yan Wang
;
Chunling Luo
;
Xiang Liu
;
Yanhong Wu
;
Suhong Pan
;
Gan Zhang
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View/Download：89/1
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Submit date：2017/07/24
Effects of drainage reorganization on phytogeographic pattern in Sino-Himalaya
期刊论文
ALPINE BOTANY, 2022, 卷号: 132, 期号: 1, 页码: 141-151
Authors:
Sun,Hang
;
Li,Zhimin
;
Landis,Jacob B.
;
Qian,Lishen
;
Zhang,Ticao
;
Deng,Tao
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Submit date：2022/04/02
Sino-Himalaya
Paleo Red River
Valley plants
Drainage reorganization
Biogeographic evolution
TERMINALIA-FRANCHETII COMBRETACEAE
MEKONG-SALWEEN DIVIDE
TANAKA-KAIYONG LINE
RIVER-CAPTURE
HENGDUAN MOUNTAINS
GENETIC-STRUCTURE
SOUTHWEST CHINA
CYTOCHROME-B
PHYLOGEOGRAPHY
EVOLUTION
Genomic basis of high-altitude adaptation in Tibetan Prunus fruit trees
期刊论文
CURRENT BIOLOGY, 2021, 卷号: 31, 期号: 17, 页码: 3848+
Authors:
Wang,Xia
;
Liu,Shengjun
;
Zuo,Hao
;
Zheng,Weikang
;
Zhang,Shanshan
;
Huang,Yue
;
Pingcuo,Gesang
;
Ying,Hong
;
Zhao,Fan
;
Li,Yuanrong
;
Liu,Junwei
;
Yi,Ting-Shuang
;
Zan,Yanjun
;
Larkin,Robert M.
;
Deng,Xiuxin
;
Zeng,Xiuli
;
Xu,Qiang
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Submit date：2022/04/02
R-PACKAGE
PHYLOGENETIC ANALYSIS
ANALYSIS TOOLKIT
ANALYSIS REVEALS
WEB SERVER
SELECTION
ELEMENT
IDENTIFICATION
ALIGNMENT
ACCURATE
Gradual genome size evolution and polyploidy in Allium from the Qinghai-Tibetan Plateau
期刊论文
ANNALS OF BOTANY, 2021
Authors:
Wang,Guangyan
;
Zhou,Ning
;
Chen,Qian
;
Yang,Ya
;
Yang,Yongping
;
Duan,Yuanwen
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Submit date：2022/04/02
Genome size evolution
polyploidy
adaptation
ecological factors
seed mass
Allium
Qinghai-Tibetan Plateau
NUCLEAR-DNA CONTENT
CONSTRAINT HYPOTHESIS
FLOW-CYTOMETRY
PLANT TRAITS
C-VALUE
MECHANISMS
ASTERACEAE
PHYLOGENY
AMARYLLIDACEAE
INFORMATION
Biogeographical divides delineated by the three-step landforms of China and the East China Sea: Insights from the phylogeography of Kerria japonica
期刊论文
JOURNAL OF BIOGEOGRAPHY, 2021, 卷号: 48, 期号: 2, 页码: 372-385
Authors:
Luo,Dong
;
Xu,Bo
;
Li,Zhi-Min
;
Sun,Hang
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Submit date：2022/04/02
biogeographical divides
East Asia
East China Sea
floristic regionalization
Kerria japonica
three‐
step landforms of China
TIBETAN PLATEAU
MOLECULAR PHYLOGEOGRAPHY
QUATERNARY CLIMATE
ASIA
DNA
CHLOROPLAST
ENDEMISM
HISTORY
PLANTS
DIVERSIFICATION
Grade of Membership models reveal geographical and environmental correlates of floristic structure in a temperate biodiversity hotspot
期刊论文
NEW PHYTOLOGIST, 2021, 卷号: 232, 期号: 3, 页码: 1424-1435
Authors:
Li,Qin
;
Sun,Hang
;
Boufford,David E.
;
Bartholomew,Bruce
;
Fritsch,Peter W.
;
Chen,Jiahui
;
Deng,Tao
;
Ree,Richard H.
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biogeographical regionalization
biotic turnover
floristic structure
Hengduan Mountains
seed plants
species motif
tropical-temperate divide
HENGDUAN MOUNTAINS
BIOGEOGRAPHICAL REGIONS
TIBETAN PLATEAU
PLANT DIVERSITY
PATTERNS
WORLDS
DIVERSIFICATION
EVOLUTION
GRADIENT
CLIMATE
Rise of herbaceous diversity at the southeastern margin of the Tibetan Plateau: First insight from fossils
期刊论文
JOURNAL OF SYSTEMATICS AND EVOLUTION, 2021
Authors:
Huang,Yong-Jiang
;
Zhu,Hai
;
Su,Tao
;
Spicer,Robert A.
;
Hu,Jin-Jin
;
Jia,Lin-Bo
;
Zhou,Zhe-Kun
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Submit date：2022/04/02
fossil carpology
Hengduan Mountains
late Pliocene
meadow
monsoon
Tibetan Plateau
LATE PLIOCENE
HENGDUAN MOUNTAINS
MOLECULAR PHYLOGENY
EVOLUTIONARY HISTORY
PLANT DIVERSITY
YUNNAN
CHINA
BIODIVERSITY
MONSOONS
CLIMATE
The complete chloroplast genome of Phlomoides younghushandii (Lamiaceae), a traditional Tibetan medicinal herb
期刊论文
MITOCHONDRIAL DNA PART B-RESOURCES, 2021, 卷号: 6, 期号: 3, 页码: 1186-1188
Authors:
Min,Dao-Zhang
;
Zhao,Fei
;
Zhang,Qiong
;
Li,Bo
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Submit date：2022/04/02
Chinese herbal medicine
Lamiodide
Phlomideae
Phlomoides
plastome
CLASSIFICATION
PHYLOGENY
New early oligocene zircon U-Pb dates for the 'Miocene' Wenshan Basin, Yunnan, China: Biodiversity and paleoenvironment
期刊论文
EARTH AND PLANETARY SCIENCE LETTERS, 2021, 卷号: 565, 页码: 116929
Authors:
Tian,Yimin
;
Spicer,Robert A.
;
Huang,Jian
;
Zhou,Zhekun
;
Su,Tao
;
Widdowson,Mike
;
Jia,Linbo
;
Li,Shihu
;
Wu,Wenjian
;
Xue,Li
;
Luo,Penghui
;
Zhang,Shitao
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Submit date：2022/04/02
biodiversity hot spot
paleoenvironment
sedimentary basin
SW China
U-Pb dating
TIBETAN PLATEAU
ASIA
PALEOCLIMATE
CLIMATE
EOCENE
TECTONICS
MONSOONS
FOSSIL
LAKE
Phaeorrhiza (Physciaceae), a new lichen genus record to China
期刊论文
PHYTOTAXA, 2021, 卷号: 510, 期号: 3, 页码: 228-238
Authors:
ZHONG,Q. I. U. Y. I.
;
ZHANG,Y. A. N. Y. U. N.
;
WANG,X. I. N. Y. U.
;
TIMDAL,E. I. N. A. R.
;
GONG,H. E. D. E.
;
WANG,Z. I. Z. H. I.
;
WANG,L. I. S. O. N. G.
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Submit date：2022/04/02
lichenized fungi
Tibetan Plateau
taxonomy
phylogeny
alpine biota
SUBUNIT RIBOSOMAL DNA
PHYLOGENY
SEQUENCE
ASCOMYCOTINA
LECANORALES
PRIMERS
NUCLEAR
FUNGI
