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中国科学院昆明植物研究所知识管理系统
Knowledge Management System of Kunming Institute of Botany,CAS
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6 could use lots of photosynthates, but contributed little to the accumulation of biomass. 4. Photosynthetic rate of P. armeniacum decreased a little at the noon, and the highest photosynthetic rate was observed at 10:00h in the greenhouse. The variation of photosynthetic rate was in the same trend as stomatal conductance. Higher relative humidity seemed to be the key for higher photosynthetic rate in P. armeniacum. 5. The photosynthetic capacity of C. flavum was statistically larger than that of P. armeniacum. The lower leaf photosynthetic capacity of P. armeniacum was related to its lower leaf nitrogen concentration,leaf phosphorus concentration and enzyme activities. Meanwhile, the extremely lower stomatal conductance and internal mesophyll conductance might greatly limit the photosynthetic capacity of P. armeniacum. The lower stomatal conductance and photosynthetic rate of Paphiopedilum might partially caused by the lack of chloroplasts in the guard cell of Paphiopedilum. Compared with C. flavum, P. armeniacum was more fond of shade environment.6. The short longevity leaf of Cypripedium had bigger photosynthetic capacity and greater potential for fast growth. But the longer LL of Paphiopedilum enhanced nutrient conservation which could compensate its lower photosynthetic capacity. The short longevity leaf of Cypripedium usually had higher photosynthetic rate per unit leaf mass and dark respiration rate, and photosynthetic capacity decreased fast with leaf age. However, for Paphiopedilum, the situation was the opposite. 7. Compared with Cypripedium, Paphiopedilum had higher water use efficiency and lower photosynthetic nitrogen use efficiency. 8. The leaf of Paphiopedilum had higher leaf construction cost and longer repayment time than that of Cypripedium. The leaf structures and physiological functions of Paphiopedilum and Cypripedium reflected the adaptation to their habitats. The leaf morphological and physiological evolution of Paphiopedilum was related to water and resource-conserving traits in the karst habitat. The leaf traits of Cypripedium were the adaptation to the environment rich in water and nutrients but easy to change with seasons.Our results provided evidence of divergent evolution of congeneric orchids under natural selection.","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&sort_by=2&isNonaffiliated=false&search_type=-1&query1=Oxidative%2BStress&order=desc&&fq=dc.project.title_filter%3APaphiopedilum%5C+and%5C+Cypripedium%5C+are%5C+close%5C+relatives%5C+belonging%5C+to%5C+the%5C+subfamily%5C+Cypripedioideae.%5C+However%2C%5C+they%5C+undergo%5C+considerable%5C+divergence%5C+in%5C+the%5C+aspects%5C+of%5C+life%5C+forms%2C%5C+leaf%5C+traits%5C+and%5C+habitats.%5C+In%5C+present%5C+study%2C%5C+leaf%5C+morphologies%5C+and%5C+anatomical%5C+structures%2C%5C+leaf%5C+lifespans%2C%5C+leaf%5C+mass%5C+per%5C+area%2C%5C+photosynthetic%5C+capacities%2C%5C+nutrient%5C+use%5C+efficiencies%2C%5C+leaf%5C+construction%5C+costs%2C%5C+and%5C+maintenance%5C+costs%5C+were%5C+investigated%5C+to%5C+understand%5C+the%5C+relationship%5C+between%5C+leaf%5C+traits%5C+and%5C+ecophysiological%5C+adaptability%5C+of%5C+the%5C+two%5C+types%5C+of%5C+plants%5C+and%5C+explore%5C+the%5C+related%5C+ecological%5C+and%5C+evolutionary%5C+significances.%5C+The%5C+results%5C+suggest%5C+that%5C%3A1.%5C+Compared%5C+with%5C+Cypripedium%2C%5C+Paphiopedilum%5C+was%5C+characterized%5C+by%5C+drought%5C+tolerance%5C+from%5C+its%5C+leaf%5C+anatomical%5C+structure%5C+including%5C+fleshy%5C+leaf%2C%5C+thicker%5C+surface%5C+cuticle%2C%5C+huge%5C+abaxial%5C+epidermis%5C+cells%2C%5C+differentiation%5C+of%5C+palisade%5C+and%5C+spongy%5C+mesophyll%5C+layers%2C%5C+the%5C+prominent%5C+of%5C+mucilaginous%5C+substances%2C%5C+supportable%5C+leaf%5C+main%5C+vein%2C%5C+lower%5C+total%5C+stoma%5C+area%5C+%5C%28%25%5C%29%2C%5C+sunken%5C+stomata%5C+and%5C+special%5C+stoma%5C+structure.%5C+Leaf%5C+morphologies%5C+and%5C+structures%5C+of%5C+Cypripedium%5C+were%5C+to%5C+the%5C+contrary%5C+of%5C+Paphiopedilum.%5C+Leaf%5C+morphologies%5C+and%5C+structures%5C+embodied%5C+the%5C+adaptation%5C+to%5C+the%5C+environment%5C+in%5C+both%5C+Paphiopedilum%5C+and%5C+Cypripedium.%5C+Our%5C+results%5C+also%5C+confirmed%5C+the%5C+previous%5C+observation%5C+that%5C+Paphiopedilum%5C+was%5C+the%5C+only%5C+genus%5C+that%5C+did%5C+not%5C+possess%5C+guard%5C+cell%5C+chloroplasts.2.%5C+The%5C+photosynthetic%5C+capacities%5C+of%5C+P.%5C+armeniacum%5C+leaves%5C+were%5C+different%5C+with%5C+different%5C+leaf%5C+ages.%5C+The%5C+highest%5C+photosynthetic%5C+capacity%5C+occurred%5C+in%5C+leaf%5C+age%5C+1%5C-2%5C+years%2C%5C+followed%5C+by%5C+1%5C+year%5C+and%5C+2%5C-4%5C+years.%5C+The%5C+highest%5C+photosynthetic%5C+capacity%5C+of%5C+C.%5C+flavum%5C+occurred%5C+in%5C+leaf%5C+age%5C+60%5C+days%2C%5C+followed%5C+by%5C+30%5C+days%2C%5C+90%5C+days%5C+and%5C+120%5C+days.%5C+3.%5C+Photosynthetic%5C+capacities%5C+of%5C+different%5C+leaf%5C+positions%5C+were%5C+mainly%5C+affected%5C+by%5C+leaf%5C+ages%5C+in%5C+P.%5C+armeniacum.%5C+The%5C+four%5C+leaves%5C+lying%5C+on%5C+the%5C+top%5C+did%5C+the%5C+most%5C+accumulation%5C+of%5C+the%5C+assimilation%5C+products%5C+in%5C+the%5C+whole%5C+plant.%5C+The%5C+leaves%5C+of%5C+sequence%5C+number%5C+%3E%5C+6%5C+could%5C+use%5C+lots%5C+of%5C+photosynthates%2C%5C+but%5C+contributed%5C+little%5C+to%5C+the%5C+accumulation%5C+of%5C+biomass.%5C+4.%5C+Photosynthetic%5C+rate%5C+of%5C+P.%5C+armeniacum%5C+decreased%5C+a%5C+little%5C+at%5C+the%5C+noon%2C%5C+and%5C+the%5C+highest%5C+photosynthetic%5C+rate%5C+was%5C+observed%5C+at%5C+10%5C%3A00h%5C+in%5C+the%5C+greenhouse.%5C+The%5C+variation%5C+of%5C+photosynthetic%5C+rate%5C+was%5C+in%5C+the%5C+same%5C+trend%5C+as%5C+stomatal%5C+conductance.%5C+Higher%5C+relative%5C+humidity%5C+seemed%5C+to%5C+be%5C+the%5C+key%5C+for%5C+higher%5C+photosynthetic%5C+rate%5C+in%5C+P.%5C+armeniacum.%5C+5.%5C+The%5C+photosynthetic%5C+capacity%5C+of%5C+C.%5C+flavum%5C+was%5C+statistically%5C+larger%5C+than%5C+that%5C+of%5C+P.%5C+armeniacum.%5C+The%5C+lower%5C+leaf%5C+photosynthetic%5C+capacity%5C+of%5C+P.%5C+armeniacum%5C+was%5C+related%5C+to%5C+its%5C+lower%5C+leaf%5C+nitrogen%5C+concentration%2Cleaf%5C+phosphorus%5C+concentration%5C+and%5C+enzyme%5C+activities.%5C+Meanwhile%2C%5C+the%5C+extremely%5C+lower%5C+stomatal%5C+conductance%5C+and%5C+internal%5C+mesophyll%5C+conductance%5C+might%5C+greatly%5C+limit%5C+the%5C+photosynthetic%5C+capacity%5C+of%5C+P.%5C+armeniacum.%5C+The%5C+lower%5C+stomatal%5C+conductance%5C+and%5C+photosynthetic%5C+rate%5C+of%5C+Paphiopedilum%5C+might%5C+partially%5C+caused%5C+by%5C+the%5C+lack%5C+of%5C+chloroplasts%5C+in%5C+the%5C+guard%5C+cell%5C+of%5C+Paphiopedilum.%5C+Compared%5C+with%5C+C.%5C+flavum%2C%5C+P.%5C+armeniacum%5C+was%5C+more%5C+fond%5C+of%5C+shade%5C+environment.6.%5C+The%5C+short%5C+longevity%5C+leaf%5C+of%5C+Cypripedium%5C+had%5C+bigger%5C+photosynthetic%5C+capacity%5C+and%5C+greater%5C+potential%5C+for%5C+fast%5C+growth.%5C+But%5C+the%5C+longer%5C+LL%5C+of%5C+Paphiopedilum%5C+enhanced%5C+nutrient%5C+conservation%5C+which%5C+could%5C+compensate%5C+its%5C+lower%5C+photosynthetic%5C+capacity.%5C+The%5C+short%5C+longevity%5C+leaf%5C+of%5C+Cypripedium%5C+usually%5C+had%5C+higher%5C+photosynthetic%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secondary metabolites are a class of small molecular compounds which are not necessary for plant growth and development in nature. They have many types and different functions. The current studies about these compounds were often focused on the chemistry, pharmacology and drug development. Research about their biological effects is few. Exploration the biological effects of plant secondary metabolites is important to study their biological function and application. Scutellarin is an active pharmaceutical ingredient extracted from Erigeron breviscapus(vant) Hand Mass. It is an important plant secondary metabolite, belonging to flavonoid. Previous studies found that some flavonoids such as quercetin, naringenin were auxin transport inhibitors in plants and they were related to plant growth and development closely. As an important flavonoid, scutellarin also plays an important role in plant growth and development is unknown. In this paper, scutellarin was selected as research object. Through a series of plant physiological, biochemical and molecular techniques to explore the biological effects of scutellarin on Arabidopsis thaliana, we get the results as follows: Scutellarin had a hormone-like effect on Arabidopsis thaliana. It promoted root elongation at low concentration obviously; however the effect disappeared at high concentration. We made a further study about it and found that scutellarin had antagonism with methyl jasmonate and 2,4-dichlorophenoxy acetic acid (2,4-D) in root elongation. It also had an influence on nitrogen metabolism. Microarray results showed that the biological effects of scutellarin had a complex relationship with plant hormone and nitrogen metabolism. These were consistent with our experimental phenomenas. All these manifested that scutellarin played an important role in plant growth and development as a similar plant hormone. We tried the experiment using some other flavonoids. It was found that not all the flavonoids had the same obvious effects on root elongation like scutellarin; the biological effects of them were closely related to their chemical structures. In addition, the other two aspects of research were also carried out in the paper. One was exploring the role of phospholipase Dδ (PLDδ) under ultraviolet radiation in Arabidopsis. Our evidences suggested knockout PLDδ intensified membrane damage induced by UV radiation. The other one was lipid data collection and calculation of acyl chain lengths of lipid molecules in Arabidopsis under senescence induced by various stresses. We showed that the acyl chains of phosphoserine (PS, a head-group class of membrane glycerolipids with very long chains of fatty acids.) lengthened with the development and senescence in Arabidopsis. In contrast, the acyl chain lengths of other major head-group classes of membrane glycerolipids subtly fluctuated.","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&sort_by=2&isNonaffiliated=false&search_type=-1&query1=Oxidative%2BStress&order=desc&&fq=dc.project.title_filter%3APlant%5C+secondary%5C+metabolites%5C+are%5C+a%5C+class%5C+of%5C+small%5C+molecular%5C+compounds%5C+which%5C+are%5C+not%5C+necessary%5C+for%5C+plant%5C+growth%5C+and%5C+development%5C+in%5C+nature.%5C+They%5C+have%5C+many%5C+types%5C+and%5C+different%5C+functions.%5C+The%5C+current%5C+studies%5C+about%5C+these%5C+compounds%5C+were%5C+often%5C+focused%5C+on%5C+the%5C+chemistry%2C%5C+pharmacology%5C+and%5C+drug%5C+development.%5C+Research%5C+about%5C+their%5C+biological%5C+effects%5C+is%5C+few.%5C+Exploration%5C+the%5C+biological%5C+effects%5C+of%5C+plant%5C+secondary%5C+metabolites%5C+is%5C+important%5C+to%5C+study%5C+their%5C+biological%5C+function%5C+and%5C+application.%5C+Scutellarin%5C+is%5C+an%5C+active%5C+pharmaceutical%5C+ingredient%5C+extracted%5C+from%5C+Erigeron%5C+breviscapus%5C%28vant%5C%29%5C+Hand%5C+Mass.%5C+It%5C+is%5C+an%5C+important%5C+plant%5C+secondary%5C+metabolite%2C%5C+belonging%5C+to%5C+flavonoid.%5C+Previous%5C+studies%5C+found%5C+that%5C+some%5C+flavonoids%5C+such%5C+as%5C+quercetin%2C%5C+naringenin%5C+were%5C+auxin%5C+transport%5C+inhibitors%5C+in%5C+plants%5C+and%5C+they%5C+were%5C+related%5C+to%5C+plant%5C+growth%5C+and%5C+development%5C+closely.%5C+As%5C+an%5C+important%5C+flavonoid%2C%5C+scutellarin%5C+also%5C+plays%5C+an%5C+important%5C+role%5C+in%5C+plant%5C+growth%5C+and%5C+development%5C+is%5C+unknown.%5C+In%5C+this%5C+paper%2C%5C+scutellarin%5C+was%5C+selected%5C+as%5C+research%5C+object.%5C+Through%5C+a%5C+series%5C+of%5C+plant%5C+physiological%2C%5C+biochemical%5C+and%5C+molecular%5C+techniques%5C+to%5C+explore%5C+the%5C+biological%5C+effects%5C+of%5C+scutellarin%5C+on%5C+Arabidopsis%5C+thaliana%2C%5C+we%5C+get%5C+the%5C+results%5C+as%5C+follows%5C%3A%5C+Scutellarin%5C+had%5C+a%5C+hormone%5C-like%5C+effect%5C+on%5C+Arabidopsis%5C+thaliana.%5C+It%5C+promoted%5C+root%5C+elongation%5C+at%5C+low%5C+concentration%5C+obviously%5C%3B%5C+however%5C+the%5C+effect%5C+disappeared%5C+at%5C+high%5C+concentration.%5C+We%5C+made%5C+a%5C+further%5C+study%5C+about%5C+it%5C+and%5C+found%5C+that%5C+scutellarin%5C+had%5C+antagonism%5C+with%5C+methyl%5C+jasmonate%5C+and%5C+2%2C4%5C-dichlorophenoxy%5C+acetic%5C+acid%5C+%5C%282%2C4%5C-D%5C%29%5C+in%5C+root%5C+elongation.%5C+It%5C+also%5C+had%5C+an%5C+influence%5C+on%5C+nitrogen%5C+metabolism.%5C+Microarray%5C+results%5C+showed%5C+that%5C+the%5C+biological%5C+effects%5C+of%5C+scutellarin%5C+had%5C+a%5C+complex%5C+relationship%5C+with%5C+plant%5C+hormone%5C+and%5C+nitrogen%5C+metabolism.%5C+These%5C+were%5C+consistent%5C+with%5C+our%5C+experimental%5C+phenomenas.%5C+All%5C+these%5C+manifested%5C+that%5C+scutellarin%5C+played%5C+an%5C+important%5C+role%5C+in%5C+plant%5C+growth%5C+and%5C+development%5C+as%5C+a%5C+similar%5C+plant%5C+hormone.%5C+We%5C+tried%5C+the%5C+experiment%5C+using%5C+some%5C+other%5C+flavonoids.%5C+It%5C+was%5C+found%5C+that%5C+not%5C+all%5C+the%5C+flavonoids%5C+had%5C+the%5C+same%5C+obvious%5C+effects%5C+on%5C+root%5C+elongation%5C+like%5C+scutellarin%5C%3B%5C+the%5C+biological%5C+effects%5C+of%5C+them%5C+were%5C+closely%5C+related%5C+to%5C+their%5C+chemical%5C+structures.%5C+In%5C+addition%2C%5C+the%5C+other%5C+two%5C+aspects%5C+of%5C+research%5C+were%5C+also%5C+carried%5C+out%5C+in%5C+the%5C+paper.%5C+One%5C+was%5C+exploring%5C+the%5C+role%5C+of%5C+phospholipase%5C+D%CE%B4%5C+%5C%28PLD%CE%B4%5C%29%5C+under%5C+ultraviolet%5C+radiation%5C+in%5C+Arabidopsis.%5C+Our%5C+evidences%5C+suggested%5C+knockout%5C+PLD%CE%B4%5C+intensified%5C+membrane%5C+damage%5C+induced%5C+by%5C+UV%5C+radiation.%5C+The%5C+other%5C+one%5C+was%5C+lipid%5C+data%5C+collection%5C+and%5C+calculation%5C+of%5C+acyl%5C+chain%5C+lengths%5C+of%5C+lipid%5C+molecules%5C+in%5C+Arabidopsis%5C+under%5C+senescence%5C+induced%5C+by%5C+various%5C+stresses.%5C+We%5C+showed%5C+that%5C+the%5C+acyl%5C+chains%5C+of%5C+phosphoserine%5C+%5C%28PS%2C%5C+a%5C+head%5C-group%5C+class%5C+of%5C+membrane%5C+glycerolipids%5C+with%5C+very%5C+long%5C+chains%5C+of%5C+fatty%5C+acids.%5C%29%5C+lengthened%5C+with%5C+the%5C+development%5C+and%5C+senescence%5C+in%5C+Arabidopsis.%5C+In%5C+contrast%2C%5C+the%5C+acyl%5C+chain%5C+lengths%5C+of%5C+other%5C+major%5C+head%5C-group%5C+classes%5C+of%5C+membrane%5C+glycerolipids%5C+subtly%5C+fluctuated."},{"jsname":"Science Research Foundation of Guizhou University[201309]","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&sort_by=2&isNonaffiliated=false&search_type=-1&query1=Oxidative%2BStress&order=desc&&fq=dc.project.title_filter%3AScience%5C+Research%5C+Foundation%5C+of%5C+Guizhou%5C+University%5C%5B201309%5C%5D"},{"jsname":"Science and Technology Foundation of Guizhou Province[[2017]2511-1]","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&sort_by=2&isNonaffiliated=false&search_type=-1&query1=Oxidative%2BStress&order=desc&&fq=dc.project.title_filter%3AScience%5C+and%5C+Technology%5C+Foundation%5C+of%5C+Guizhou%5C+Province%5C%5B%5C%5B2017%5C%5D2511%5C-1%5C%5D"},{"jsname":"Science and Technology Research Program of Kunming Institute of Botany, the Chinese Academy of Sciences[KIB2017006]","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&sort_by=2&isNonaffiliated=false&search_type=-1&query1=Oxidative%2BStress&order=desc&&fq=dc.project.title_filter%3AScience%5C+and%5C+Technology%5C+Research%5C+Program%5C+of%5C+Kunming%5C+Institute%5C+of%5C+Botany%2C%5C+the%5C+Chinese%5C+Academy%5C+of%5C+Sciences%5C%5BKIB2017006%5C%5D"},{"jsname":"Seeds contain the entire genetic information of plants, which enable to develop the technology of seed preservation for the conservation of plant diversity. The mechanism of cell death in seeds, which are induced by ageing of orthodox seeds and desiccation of recalcitrant and intermediate seeds are two important areas in seed biology but poorly understood.Those two areas were investigated in this dissertation, and Pisum sativum, Camellia sinensis var. assamica, Castanea mollissima were chosen to represent orthodox, intermediate and recalcitrant seeds respectively as studied models. Biophysical, biochemical and molecular methods were employed to investigate the cell death mechanism during seeds ageing and desiccation.First of all, Ageing induced porgrammed cell death in seeds.The morphology change, nucleic acid integrity, and gene expression during ageing of Pisum sativum seeds were investigated.The data above confirmed that PCD is an underlying mechanism of seed mortality. Moreover, glutathione played an important function during seeds ageing.An increase in glutathione redox potential (EGSSG/2GSH) while seed lost viability was discovered during ageing of P. sativum seeds. Seed viability decreased 50% when EGSSG/2GSH attained values of -180 to -160 mV, which proved that EGSSG/2GSH was a marker for seeds viability. Secondely,Reactive oxygen species plays different roles in the seed germination after desiccation. After different desiccation treatments on the tea (C. sinensis var. assamica) seeds, the relationship between ROS dose and the viability of seeds after desication treatment was investigated. The results demonstrated that the presence of ROS could released the damage of desiccation on the tea seeds. Thridly,the composition of membrane lipid species and the change during desiccation in seeds with different storage behavior. Cell membrane is very sensitive to environment, and adjust the composition of lipid molecules is one of the main methord for cell to response the environmental changes. There is a rule that phosphatidic acid (PA), with special structure, is very sensitive to temperature and water stress, and the increase of PA content indicates the damage of the cell membrane. ESI/MS-MS technology was used to analysis and compare the composition and changes membrane lipid species during desiccation treatments of Pisum sativum, Camellia sinensis var. assamica, Castanea mollissima seeds which represented orthodox, intermediate and recalcitrant seeds respectively. The results showed that the responses of PA to dehydration in embryonic axis of orhtordox and recalcitrant seeds were different. The percentage increasement of PA in orthodox seed during desiccation was not incredible and the level of PA decreased and achieved the same level as controled, while PA level increased dramatically without showing any decrease trend in recalcitrant seed, which indicated that the damage to membrane of recalcitrant seeds was inevitable. Those results indicted that the dehydration might induced reversible memebrane damage in the orthodox seed, which may result in no affect on the seed viability, while it might induced irreversible membrane damage to the recalcitrant seed, which inducned the dramatically reduce of viabiligy and cell death in the end. It was the first time to investigate and analysis systematically the composition and changes of membrane lipids of different storage behavior seeds during desiccation. It was first time to report the relationship between PA and the seeds storeage and the change of PA level in seeds could be used as the marker to diagnose.the property of seeds for desiccation sensitivity or desiccation tolerance","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&sort_by=2&isNonaffiliated=false&search_type=-1&query1=Oxidative%2BStress&order=desc&&fq=dc.project.title_filter%3ASeeds%5C+contain%5C+the%5C+entire%5C+genetic%5C+information%5C+of%5C+plants%2C%5C+which%5C+enable%5C+to%5C+develop%5C+the%5C+technology%5C+of%5C+seed%5C+preservation%5C+for%5C+the%5C+conservation%5C+of%5C+plant%5C+diversity.%5C+The%5C+mechanism%5C+of%5C+cell%5C+death%5C+in%5C+seeds%2C%5C+which%5C+are%5C+induced%5C+by%5C+ageing%5C+of%5C+orthodox%5C+seeds%5C+and%5C+desiccation%5C+of%5C+recalcitrant%5C+and%5C+intermediate%5C+seeds%5C+are%5C+two%5C+important%5C+areas%5C+in%5C+seed%5C+biology%5C+but%5C+poorly%5C+understood.Those%5C+two%5C+areas%5C+were%5C+investigated%5C+in%5C+this%5C+dissertation%2C%5C+and%5C+Pisum%5C+sativum%2C%5C+Camellia%5C+sinensis%5C+var.%5C+assamica%2C%5C+Castanea%5C+mollissima%5C+were%5C+chosen%5C+to%5C+represent%5C+orthodox%2C%5C+intermediate%5C+and%5C+recalcitrant%5C+seeds%5C+respectively%5C+as%5C+studied%5C+models.%5C+Biophysical%2C%5C+biochemical%5C+and%5C+molecular%5C+methods%5C+were%5C+employed%5C+to%5C+investigate%5C+the%5C+cell%5C+death%5C+mechanism%5C+during%5C+seeds%5C+ageing%5C+and%5C+desiccation.First%5C+of%5C+all%2C%5C+Ageing%5C+induced%5C+porgrammed%5C+cell%5C+death%5C+in%5C+seeds.The%5C+morphology%5C+change%2C%5C+nucleic%5C+acid%5C+integrity%2C%5C+and%5C+gene%5C+expression%5C+during%5C+ageing%5C+of%5C+Pisum%5C+sativum%5C+seeds%5C+were%5C+investigated.The%5C+data%5C+above%5C+confirmed%5C+that%5C+PCD%5C+is%5C+an%5C+underlying%5C+mechanism%5C+of%5C+seed%5C+mortality.%5C+Moreover%2C%5C+glutathione%5C+played%5C+an%5C+important%5C+function%5C+during%5C+seeds%5C+ageing.An%5C+increase%5C+in%5C+glutathione%5C+redox%5C+potential%5C+%5C%28EGSSG%5C%2F2GSH%5C%29%5C+while%5C+seed%5C+lost%5C+viability%5C+was%5C+discovered%5C+during%5C+ageing%5C+of%5C+P.%5C+sativum%5C+seeds.%5C+Seed%5C+viability%5C+decreased%5C+50%25%5C+when%5C+EGSSG%5C%2F2GSH%5C+attained%5C+values%5C+of%5C+%5C-180%5C+to%5C+%5C-160%5C+mV%2C%5C+which%5C+proved%5C+that%5C+EGSSG%5C%2F2GSH%5C+was%5C+a%5C+marker%5C+for%5C+seeds%5C+viability.%5C+Secondely%2CReactive%5C+oxygen%5C+species%5C+plays%5C+different%5C+roles%5C+in%5C+the%5C+seed%5C+germination%5C+after%5C+desiccation.%5C+After%5C+different%5C+desiccation%5C+treatments%5C+on%5C+the%5C+tea%5C+%5C%28C.%5C+sinensis%5C+var.%5C+assamica%5C%29%5C+seeds%2C%5C+the%5C+relationship%5C+between%5C+ROS%5C+dose%5C+and%5C+the%5C+viability%5C+of%5C+seeds%5C+after%5C+desication%5C+treatment%5C+was%5C+investigated.%5C+The%5C+results%5C+demonstrated%5C+that%5C+the%5C+presence%5C+of%5C+ROS%5C+could%5C+released%5C+the%5C+damage%5C+of%5C+desiccation%5C+on%5C+the%5C+tea%5C+seeds.%5C+Thridly%2Cthe%5C+composition%5C+of%5C+membrane%5C+lipid%5C+species%5C+and%5C+the%5C+change%5C+during%5C+desiccation%5C+in%5C+seeds%5C+with%5C+different%5C+storage%5C+behavior.%5C+Cell%5C+membrane%5C+is%5C+very%5C+sensitive%5C+to%5C+environment%2C%5C+and%5C+adjust%5C+the%5C+composition%5C+of%5C+lipid%5C+molecules%5C+is%5C+one%5C+of%5C+the%5C+main%5C+methord%5C+for%5C+cell%5C+to%5C+response%5C+the%5C+environmental%5C+changes.%5C+There%5C+is%5C+a%5C+rule%5C+that%5C+phosphatidic%5C+acid%5C+%5C%28PA%5C%29%2C%5C+with%5C+special%5C+structure%2C%5C+is%5C+very%5C+sensitive%5C+to%5C+temperature%5C+and%5C+water%5C+stress%2C%5C+and%5C+the%5C+increase%5C+of%5C+PA%5C+content%5C+indicates%5C+the%5C+damage%5C+of%5C+the%5C+cell%5C+membrane.%5C+ESI%5C%2FMS%5C-MS%5C+technology%5C+was%5C+used%5C+to%5C+analysis%5C+and%5C+compare%5C+the%5C+composition%5C+and%5C+changes%C2%A0membrane%5C+lipid%5C+species%5C+during%5C+desiccation%5C+treatments%5C+of%5C+Pisum%5C+sativum%2C%5C+Camellia%5C+sinensis%5C+var.%5C+assamica%2C%5C+Castanea%5C+mollissima%5C+seeds%5C+which%5C+represented%5C+orthodox%2C%5C+intermediate%5C+and%5C+recalcitrant%5C+seeds%5C+respectively.%5C+The%5C+results%5C+showed%5C+that%5C+the%5C+responses%5C+of%5C+PA%5C+to%5C+dehydration%5C+in%5C+embryonic%5C+axis%5C+of%5C+orhtordox%5C+and%5C+recalcitrant%5C+seeds%5C+were%5C+different.%5C+The%5C+percentage%5C+increasement%5C+of%5C+PA%5C+in%5C+orthodox%5C+seed%5C+during%5C+desiccation%5C+was%5C+not%5C+incredible%5C+and%5C+the%5C+level%5C+of%5C+PA%5C+decreased%5C+and%5C+achieved%5C+the%5C+same%5C+level%5C+as%5C+controled%2C%5C+while%5C+PA%5C+level%5C+increased%5C+dramatically%5C+without%5C+showing%5C+any%5C+decrease%5C+trend%5C+in%5C+recalcitrant%5C+seed%2C%5C+which%5C+indicated%5C+that%5C+the%5C+damage%5C+to%5C+membrane%5C+of%5C+recalcitrant%5C+seeds%5C+was%5C+inevitable.%C2%A0Those%5C+results%5C+indicted%5C+that%5C+the%5C+dehydration%5C+might%5C+induced%5C+reversible%5C+memebrane%5C+damage%5C+in%5C+the%5C+orthodox%5C+seed%2C%5C+which%5C+may%5C+result%5C+in%5C+no%5C+affect%5C+on%5C+the%5C+seed%5C+viability%2C%5C+while%5C+it%5C+might%5C+induced%5C+irreversible%5C+membrane%5C+damage%5C+to%5C+the%5C+recalcitrant%5C+seed%2C%5C+which%5C+inducned%5C+the%5C+dramatically%5C+reduce%5C+of%5C+viabiligy%5C+and%5C+cell%5C+death%5C+in%5C+the%5C+end.%5C+It%5C+was%5C+the%5C+first%5C+time%5C+to%5C+investigate%5C+and%5C+analysis%5C+systematically%5C+the%5C+composition%5C+and%5C+changes%5C+of%5C+membrane%5C+lipids%5C+of%5C+different%5C+storage%5C+behavior%5C+seeds%5C+during%5C+desiccation.%5C+It%5C+was%5C+first%5C+time%5C+to%5C+report%5C+the%5C+relationship%5C+between%5C+PA%5C+and%5C+the%5C+seeds%5C+storeage%5C+and%5C+the%5C+change%5C+of%5C+PA%5C+level%5C+in%5C+seeds%5C+could%5C+be%5C+used%5C+as%5C+the%5C+marker%5C+to%5C+diagnose.the%5C+property%5C+of%5C+seeds%5C+for%5C+desiccation%5C+sensitivity%5C+or%5C+desiccation%5C+tolerance"},{"jsname":"Southeast Asia Biodiversity Research Institute, Chinese Academy of Sciences[2015CASEABRIRG001]","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&sort_by=2&isNonaffiliated=false&search_type=-1&query1=Oxidative%2BStress&order=desc&&fq=dc.project.title_filter%3ASoutheast%5C+Asia%5C+Biodiversity%5C+Research%5C+Institute%2C%5C+Chinese%5C+Academy%5C+of%5C+Sciences%5C%5B2015CASEABRIRG001%5C%5D"},{"jsname":"The membrane system of cell performs many important functions, such as separates cells from the environment, keeps the biochemical reactions in order ect.. The integrity of membrane is very important for plants to survive, especially under the environmental stress. Among all environmental factors, temperature has the closest relationship with membrane and intensively study on this area has been reported. Most researches are mainly focused on the relationship between the composition of fatty acid about membrane and low temperature, while that with high temperature are rare. Nowadays, the increasing concentration of CO2 resulted in increasing temperature and high temperature has become an important inhibition to crop productivity. Thus, it’s necessary and emergent to study the relationship between membrane lipids and high temperature.In the present dissertation, Arabidopsis and its high temperature sensitive mutant were chosen to study the relationship between membrane lipids and high temperature. The ESI-MS/MS was used to examine the composition of membrane lipids. High temperature includes two categories, one is heat stress and the other is moderate heat stress. Heat stress can be divided into two processes: with and without heat acclimation. Five results have been obtained grounding on these works. Firstly, different change models of membrane lipids during heat stress and moderate stress had been found. The degradation of membrane lipids during moderate heat stress was controlled, while that of heat stress was out of control. During moderate heat stress, the degradation mainly happened on chloroplast, such as DGDG and PG, especially those lipids which has polyunsaturated fatty acids. Under heat stress, the degradation about plasma membrane and chloroplast membrane shared same rates. Secondly, the degradation of membrane lipids was reduced when plants had experienced heat acclimation before heat stress, and this change had nothing to do with accumulation of HSP101. The results suggested the acquired thermo-tolerance not only had related with HSP101, but also with membrane lipids. Thirdly, the amount of phosphatidic acid (PA) played an important role during heat stress. If the amount of PA rose to proper extent, it benefited the plants, while if it rose to high level, it destroyed the membrane structure. At last, the HSP101 mutant had higher ratio of polyunsaturated fatty acids/ saturated fatty acids than that of wild Arabidopsis under long term moderate heat stress. The dissertation also included other two parts: the drought-tolerance of Thellungiella halophila and the chemical structure and bioactivity of the second metabolites from endophytes, which were isolated from Trewia nudiflor. Thellungiella halophila shared the same characteristic with Arabidopsis in many aspects, such as dwarf phenotype, short life cycle, fertility and small genome. The research indicated that at cDNA level, they were also very similar. Besides these Thellungiella halophila was more tolerant to stress condition. The previous research about Thellungiella halophila mainly focused on the high-salinity stress, and the researches of drought stress were rare. In this dissertation we focused on the drought-resistance of Thellungiella halophila. Compare to Arabidopsis, Thellungiella halophila could keep water content in high level, more resist to ROS, good photosynthesis activity and keep the membrane system integrity under drought stress. It was interesting that the substances, which rose when Arabidopsis under stress, were at high level in normal Thellungiella halophila, such as: proline, ABA. The degradation of membrane lipids mainly happened on chloroplast membrane of Arabidopsis. In contrast, the membrane of Thellungiella halophila didn’t change. All these evidence indicated that Thellungiella halophila was more drought-tolerant than Arabidopsis. During the research about the chemical structure and bioactivity of the second metabolites from endophytes, which were isolated from Trewia nudiflor, we isolated 46 endophytes from different parts of plants . 34 species of them were selected for bioactivity test, and the bioactivity test show that 50% of them have some bioactivity. We also isolated 24 compounds from 6 endophytes, and 22 of them have been identified by spectra data, including: macrolides, azaphilones, anthraquinones, and steroids. 8 of them are novel compounds. Judging from results, we know the Trewia nudiflor is good resources to isolate endophytes and the endophytes are good resources to search for novel and bioactivity 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reaction of transphosphatidylation is which phospholipase D catalyzes hydrolytic cleavage of the terminal phosphate diester bond of glycerophosphatides, transfer the phosphatidyl moiety of a phospholipids to a primary alcohol or water, producing phosphatidyl alcohol or phosphatidic acid (PtdOH). Although this reaction plays important role in regulating physical process in plants and anminals, the pathway involved in and detailed regulation mechanism are still unknown or not clear. There are three factors which can affect transphosphatidylation, substrates, enzymes and products. This paper intended to uncover the physical effect of the reaction in detail by changing two indispensable factors, the type of substrate and the PLDs. This paper consist of three researches, the first one, changing the accept substrate of the reaction aims to study the relationship between the structure of alcohols and the physical effect, to reveal the significance of the reaction; secondly, using genetic methods to regulate the expression of PLDs mediating transphosphatidylation intends to study its roles in regulating senescence; thirdly, reducing mostly PA derived from PLDs by addition 1-Butanol try to study the effect of PLD-derived PA on phosphate starvation. 1. Initial research of the effect of alcohols on the palnt growth. In this study, we study on the effect of alcohols on Arabidopsis seed germination, seeding growth and membrane lipids molecules, the metabolic procees and signaling pathway invoved in plant responding to 1-Butanol. According to the results we observed, the toxicity of alcohols and its polarity is positively correlated; the toxicity of alcohols to plants may be related with the butanoate and propanoate metabolic pathway rather than transphosphatidylation, the increase of free IAA is contributed to the resistance to 1-Butanol for WS ecotype Arabidopsis thaliana. Futhermore, five 1-Butanol insensitive mutants have obtained and the mutant sites has identified. Under the same concentration 1-Butanol stress, the five mutants are in better condition no matter physical and chemical level or cellular levels compared to wild-type Arabidopsis thaliana. It is believed that the five mutants definitely provide direct evidence for us to uncover the mechanism of the toxicity of alcohols to plants. 2. The change pattern of the membrane lipid molecules responding to the phytohormones-promoted detached Arabidopsis leaves and the role of PLDα1 and PLDδ in regulating this process. The reason for PLDα1-antisense mutant and PLDδ-knockout mutant delaying phytohormone-promoted senescence may be PLDα1 and PLDδ are response to the ABA and ethylene sinaling; PLDα1-or PLDδ-derived PA contributes to reactive oxygen species (ROS) accumulation; PLDα1-antisense mutant and PLDδ-knockout mutant have higher level of indole-3-acrtic acid (IAA) and zeatin riboside (ZR) level but lower level of abscisic acid (ABA) and jasmonic acid (JA) compared to wild-type Col and wild-type WS, respectively, which is favorable to retard the phytohormone-promoted detached leaves senescence. 3. Application tert-butanol increase the efficiency of phosphate utilization and absord when the phosphate-limited. The seedinges phosphate-starved or not exposed to 1-Butanol was subjected to lethal stress. However, in low phosphate condition, application of tert-butanol lower anthocyanin accumulation, increase the phosphate levels and the siliques number, in a word, tert-butanol attenuates the symptom of phosphate-starved induced. It is suggested that tert-Butanol can increase the utilization and absord efficiency of the limited phosphate, however, more evidences are needed to prove the mechanism.","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&sort_by=2&isNonaffiliated=false&search_type=-1&query1=Oxidative%2BStress&order=desc&&fq=dc.project.title_filter%3AThe%5C+reaction%5C+of%5C+transphosphatidylation%5C+is%5C+which%5C+phospholipase%5C+D%5C+catalyzes%5C+hydrolytic%5C+cleavage%5C+of%5C+the%5C+terminal%5C+phosphate%5C+diester%5C+bond%5C+of%5C+glycerophosphatides%2C%5C+transfer%5C+the%5C+phosphatidyl%5C+moiety%5C+of%5C+a%5C+phospholipids%5C+to%5C+a%5C+primary%5C+alcohol%5C+or%5C+water%2C%5C+producing%5C+phosphatidyl%5C+alcohol%5C+or%5C+phosphatidic%5C+acid%5C+%5C%28PtdOH%5C%29.%5C+Although%5C+this%5C+reaction%5C+plays%5C+important%5C+role%5C+in%5C+regulating%5C+physical%5C+process%5C+in%5C+plants%5C+and%5C+anminals%2C%5C+the%5C+pathway%5C+involved%5C+in%5C+and%5C+detailed%5C+regulation%5C+mechanism%5C+are%5C+still%5C+unknown%5C+or%5C+not%5C+clear.%5C+There%5C+are%5C+three%5C+factors%5C+which%5C+can%5C+affect%5C+transphosphatidylation%2C%5C+substrates%2C%5C+enzymes%5C+and%5C+products.%5C+This%5C+paper%5C+intended%5C+to%5C+uncover%5C+the%5C+physical%5C+effect%5C+of%5C+the%5C+reaction%5C+in%5C+detail%5C+by%5C+changing%5C+two%5C+indispensable%5C+factors%2C%5C+the%5C+type%5C+of%5C+substrate%5C+and%5C+the%5C+PLDs.%5C+This%5C+paper%5C+consist%5C+of%C2%A0three%5C+researches%2C%5C+the%5C+first%5C+one%2C%5C+changing%5C+the%5C+accept%5C+substrate%5C+of%5C+the%5C+reaction%5C+aims%5C+to%5C+study%5C+the%5C+relationship%5C+between%5C+the%5C+structure%5C+of%5C+alcohols%5C+and%5C+the%5C+physical%5C+effect%2C%5C+to%5C+reveal%5C+the%5C+significance%5C+of%5C+the%5C+reaction%5C%3B%5C+secondly%2C%5C+using%5C+genetic%5C+methods%5C+to%5C+regulate%5C+the%5C+expression%5C+of%5C+PLDs%5C+mediating%5C+transphosphatidylation%5C+intends%5C+to%5C+study%5C+its%5C+roles%5C+in%5C+regulating%5C+senescence%5C%3B%5C+thirdly%2C%5C+reducing%5C+mostly%5C+PA%5C+derived%5C+from%5C+PLDs%5C+by%5C+addition%5C+1%5C-Butanol%5C+try%5C+to%5C+study%5C+the%5C+effect%5C+of%5C+PLD%5C-derived%5C+PA%5C+on%5C+phosphate%5C+starvation.%5C+1.%5C+Initial%5C+research%5C+of%5C+the%5C+effect%5C+of%5C+alcohols%5C+on%5C+the%5C+palnt%5C+growth.%5C+In%5C+this%5C+study%2C%5C+we%5C+study%5C+on%5C+the%5C+effect%5C+of%5C+alcohols%5C+on%5C+Arabidopsis%5C+seed%5C+germination%2C%5C+seeding%5C+growth%5C+and%5C+membrane%5C+lipids%5C+molecules%2C%5C+the%5C+metabolic%5C+procees%5C+and%5C+signaling%5C+pathway%5C+invoved%5C+in%5C+plant%5C+responding%5C+to%5C+1%5C-Butanol.%5C+According%5C+to%5C+the%5C+results%5C+we%5C+observed%2C%5C+the%5C+toxicity%5C+of%5C+alcohols%5C+and%5C+its%5C+polarity%5C+is%5C+positively%5C+correlated%5C%3B%5C+the%5C+toxicity%5C+of%5C+alcohols%5C+to%5C+plants%5C+may%5C+be%5C+related%5C+with%5C+the%5C+butanoate%5C+and%5C+propanoate%5C+metabolic%5C+pathway%5C+rather%5C+than%5C+transphosphatidylation%2C%5C+the%5C+increase%5C+of%5C+free%5C+IAA%5C+is%5C+contributed%5C+to%5C+the%5C+resistance%5C+to%5C+1%5C-Butanol%5C+for%5C+WS%5C+ecotype%5C+Arabidopsis%5C+thaliana.%5C+Futhermore%2C%5C+five%5C+1%5C-Butanol%5C+insensitive%5C+mutants%5C+have%5C+obtained%5C+and%5C+the%5C+mutant%5C+sites%5C+has%5C+identified.%5C+Under%5C+the%5C+same%5C+concentration%5C+1%5C-Butanol%5C+stress%2C%5C+the%5C+five%5C+mutants%5C+are%5C+in%5C+better%5C+condition%5C+no%5C+matter%5C+physical%5C+and%5C+chemical%5C+level%5C+or%5C+cellular%5C+levels%5C+compared%5C+to%5C+wild%5C-type%5C+Arabidopsis%5C+thaliana.%5C+It%5C+is%5C+believed%5C+that%5C+the%5C+five%5C+mutants%5C+definitely%5C+provide%5C+direct%5C+evidence%5C+for%5C+us%5C+to%5C+uncover%5C+the%5C+mechanism%5C+of%5C+the%5C+toxicity%5C+of%5C+alcohols%5C+to%5C+plants.%5C+2.%5C+The%5C+change%5C+pattern%5C+of%5C+the%5C+membrane%5C+lipid%5C+molecules%5C+responding%5C+to%5C+the%5C+phytohormones%5C-promoted%5C+detached%5C+Arabidopsis%5C+leaves%5C+and%5C+the%5C+role%5C+of%5C+PLD%CE%B11%5C+and%5C+PLD%CE%B4%5C+in%5C+regulating%5C+this%5C+process.%5C+The%5C+reason%5C+for%5C+PLD%CE%B11%5C-antisense%5C+mutant%5C+and%5C+PLD%CE%B4%5C-knockout%5C+mutant%5C+delaying%5C+phytohormone%5C-promoted%5C+senescence%5C+may%5C+be%5C+PLD%CE%B11%5C+and%5C+PLD%CE%B4%5C+are%5C+response%5C+to%5C+the%5C+ABA%5C+and%5C+ethylene%5C+sinaling%5C%3B%5C+PLD%CE%B11%5C-or%5C+PLD%CE%B4%5C-derived%5C+PA%5C+contributes%5C+to%5C+reactive%5C+oxygen%5C+species%5C+%5C%28ROS%5C%29%5C+accumulation%5C%3B%5C+PLD%CE%B11%5C-antisense%5C+mutant%5C+and%5C+PLD%CE%B4%5C-knockout%5C+mutant%5C+have%5C+higher%5C+level%5C+of%5C+indole%5C-3%5C-acrtic%5C+acid%5C+%5C%28IAA%5C%29%5C+and%5C+zeatin%5C+riboside%5C+%5C%28ZR%5C%29%5C+level%5C+but%5C+lower%5C+level%5C+of%5C+abscisic%5C+acid%5C+%5C%28ABA%5C%29%5C+and%5C+jasmonic%5C+acid%5C+%5C%28JA%5C%29%5C+compared%5C+to%5C+wild%5C-type%5C+Col%5C+and%5C+wild%5C-type%5C+WS%2C%5C+respectively%2C%5C+which%5C+is%5C+favorable%5C+to%5C+retard%5C+the%5C+phytohormone%5C-promoted%5C+detached%5C+leaves%5C+senescence.%5C+3.%5C+Application%5C+tert%5C-butanol%5C+increase%5C+the%5C+efficiency%5C+of%5C+phosphate%5C+utilization%5C+and%5C+absord%5C+when%5C+the%5C+phosphate%5C-limited.%5C+The%5C+seedinges%5C+phosphate%5C-starved%5C+or%5C+not%5C+exposed%5C+to%5C+1%5C-Butanol%5C+was%5C+subjected%5C+to%5C+lethal%5C+stress.%5C+However%2C%5C+in%5C+low%5C+phosphate%5C+condition%2C%5C+application%5C+of%5C+tert%5C-butanol%5C+lower%5C+anthocyanin%5C+accumulation%2C%5C+increase%5C+the%5C+phosphate%5C+levels%5C+and%5C+the%5C+siliques%5C+number%2C%5C+in%5C+a%5C+word%2C%5C+tert%5C-butanol%5C+attenuates%5C+the%5C+symptom%5C+of%5C+phosphate%5C-starved%5C+induced.%5C+It%5C+is%5C+suggested%5C+that%5C+tert%5C-Butanol%5C+can%5C+increase%5C+the%5C+utilization%5C+and%5C+absord%5C+efficiency%5C+of%5C+the%5C+limited%5C+phosphate%2C%5C+however%2C%5C+more%5C+evidences%5C+are%5C+needed%5C+to%5C+prove%5C+the%5C+mechanism."},{"jsname":"The thesis includes four chapters: Chapters 1 and 2 cover bioactive constituents of two traditional Chininese medicines of Ding-Zhi-Yuan prescription: Acorus tatarinowii Schott. and Poria cum Radix Pini. Chapter 3 introduces chemical components of flavonoids from the gum of Cerasus conradinae. In the last chapter, the research progress of Acorus was reviewed. As a result, 60 compounds were isolated from these plants, and eight of them were identified as new ones. Their structures were established on the basis of spectroscopic data. The types of these compounds include phenylpropanoids, lignans, flavonoids, sesquiterpenoids, alkaloids, and steroids.Chapter 1 introduced the chemical constitutents of Acorus tatarinowii Schott. Acorus tatarinowii Schott is a famous traditional Chinese medicine possessing anticonvulsive,spasmolytic, and neuroprotective effects. To study its pharmacological mechanism, 47 compounds, including 7 new ones, were isolated from this herb. AlphaScreen cAMP assay showed that six phenylpropanoids significantly increased cAMP level at the concentration of 50 µM. Two novel spiro-alkaloids inhibited high glucose-induced ROS production in mesangial cells. Besides, their anti-oxidant activities were dose-dependent and reached to the maximum at 10 μM and 50 μM, respectively.Chapter 2 introduced the chemical constitutents of Poria cum Radix Pin. Poria cum Radix Pini is the xylem of Poria cocos (Schw.) Wolf. As a traditional Chinese medicine, it has the effects for treatment of palpitation, forgettery, insomnia and other diseases. Chemical investigation about it resulted in 9 compounds and 1 compound was elucidated to be new. Chater 3 introduced the chemical constituents of flavonoids from the gum of Cerasus conradina.ere isolated from the gum ofCerasus conradinae. The fruits of Cerasus conradina were used to deal with heart failure, beriberi and edema. Because of frostibe, pest, and mechanical damage, Cerasus conradin excrete lots of gum in winter. To investigate the chemical constituents and the chemical defence function of the gum, 4 flavonoids with different structure types w. Chapter 4 reviewed the progress of the studies on chemistry and bioactivity of chemical constituents from Acorus","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&sort_by=2&isNonaffiliated=false&search_type=-1&query1=Oxidative%2BStress&order=desc&&fq=dc.project.title_filter%3AThe%5C+thesis%5C+includes%5C+four%5C+chapters%5C%3A%5C+Chapters%5C+1%5C+and%5C+2%5C+cover%5C+bioactive%5C+constituents%5C+of%5C+two%5C+traditional%5C+Chininese%5C+medicines%5C+of%5C+Ding%5C-Zhi%5C-Yuan%5C+prescription%5C%3A%5C+Acorus%5C+tatarinowii%5C+Schott.%5C+and%5C+Poria%5C+cum%5C+Radix%5C+Pini.%5C+Chapter%5C+3%5C+introduces%5C+chemical%5C+components%5C+of%5C+flavonoids%5C+from%5C+the%5C+gum%5C+of%5C+Cerasus%5C+conradinae.%5C+In%5C+the%5C+last%5C+chapter%2C%5C+the%5C+research%5C+progress%5C+of%5C+Acorus%5C+was%5C+reviewed.%5C+As%5C+a%5C+result%2C%5C+60%5C+compounds%5C+were%5C+isolated%5C+from%5C+these%5C+plants%2C%5C+and%5C+eight%5C+of%5C+them%5C+were%5C+identified%5C+as%5C+new%5C+ones.%5C+Their%5C+structures%5C+were%5C+established%5C+on%5C+the%5C+basis%5C+of%5C+spectroscopic%5C+data.%5C+The%5C+types%5C+of%5C+these%5C+compounds%5C+include%5C+phenylpropanoids%2C%5C+lignans%2C%5C+flavonoids%2C%5C+sesquiterpenoids%2C%5C+alkaloids%2C%5C+and%5C+steroids.Chapter%5C+1%5C+introduced%5C+the%5C+chemical%5C+constitutents%5C+of%5C+Acorus%5C+tatarinowii%5C+Schott.%5C+Acorus%5C+tatarinowii%5C+Schott%5C+is%5C+a%5C+famous%5C+traditional%5C+Chinese%5C+medicine%5C+possessing%5C+anticonvulsive%2Cspasmolytic%2C%5C+and%5C+neuroprotective%5C+effects.%5C+To%5C+study%5C+its%5C+pharmacological%5C+mechanism%2C%5C+47%5C+compounds%2C%5C+including%5C+7%5C+new%5C+ones%2C%5C+were%5C+isolated%5C+from%5C+this%5C+herb.%5C+AlphaScreen%5C+cAMP%5C+assay%5C+showed%5C+that%5C+six%5C+phenylpropanoids%5C+significantly%5C+increased%5C+cAMP%5C+level%5C+at%5C+the%5C+concentration%5C+of%5C+50%5C+%C2%B5M.%5C+Two%5C+novel%5C+spiro%5C-alkaloids%5C+inhibited%5C+high%5C+glucose%5C-induced%5C+ROS%5C+production%5C+in%5C+mesangial%5C+cells.%5C+Besides%2C%5C+their%5C+anti%5C-oxidant%5C+activities%5C+were%5C+dose%5C-dependent%5C+and%5C+reached%5C+to%5C+the%5C+maximum%5C+at%5C+10%5C+%CE%BCM%5C+and%5C+50%5C+%CE%BCM%2C%5C+respectively.Chapter%5C+2%5C+introduced%5C+the%5C+chemical%5C+constitutents%5C+of%5C+Poria%5C+cum%5C+Radix%5C+Pin.%5C+Poria%5C+cum%5C+Radix%5C+Pini%5C+is%5C+the%5C+xylem%5C+of%5C+Poria%5C+cocos%5C+%5C%28Schw.%5C%29%5C+Wolf.%5C+As%5C+a%5C+traditional%5C+Chinese%5C+medicine%2C%5C+it%5C+has%5C+the%5C+effects%5C+for%5C+treatment%5C+of%5C+palpitation%2C%5C+forgettery%2C%5C+insomnia%5C+and%5C+other%5C+diseases.%5C+Chemical%5C+investigation%5C+about%5C+it%5C+resulted%5C+in%5C+9%5C+compounds%5C+and%5C+1%5C+compound%5C+was%5C+elucidated%5C+to%5C+be%5C+new.%5C+Chater%5C+3%5C+introduced%5C+the%5C+chemical%5C+constituents%5C+of%5C+flavonoids%5C+from%5C+the%5C+gum%5C+of%5C+Cerasus%5C+conradina.ere%5C+isolated%5C+from%5C+the%5C+gum%5C+ofCerasus%5C+conradinae.%5C+The%5C+fruits%5C+of%5C+Cerasus%5C+conradina%5C+were%5C+used%5C+to%5C+deal%5C+with%5C+heart%5C+failure%2C%5C+beriberi%5C+and%5C+edema.%5C+Because%5C+of%5C+frostibe%2C%5C+pest%2C%5C+and%5C+mechanical%5C+damage%2C%5C+Cerasus%5C+conradin%5C+excrete%5C+lots%5C+of%5C+gum%5C+in%5C+winter.%5C+To%5C+investigate%5C+the%5C+chemical%5C+constituents%5C+and%5C+the%5C+chemical%5C+defence%5C+function%5C+of%5C+the%5C+gum%2C%5C+4%5C+flavonoids%5C+with%5C+different%5C+structure%5C+types%5C+w.%5C+Chapter%5C+4%5C+reviewed%5C+the%5C+progress%5C+of%5C+the%5C+studies%5C+on%5C+chemistry%5C+and%5C+bioactivity%5C+of%5C+chemical%5C+constituents%5C+from%5C+Acorus"},{"jsname":"lastIndexed","jscount":"2020-01-25"}],"Funding Project","dc.project.title_filter")'>
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Anti-arthritis effects of (E)-2,4-bis(p-hydroxyphenyl)-2-butenal through inhibition of STAT3 pathway
期刊论文
出版物, 3111, 期号: 0, 页码: 1-50
Authors:
Jung Ok Ban
;
Dae Hwan Kim
;
Hee Pom Lee
;
Chul Ju Hwang
;
Jung-Hyun Shim
;
Dae Joong Kim
;
Tae Myoung Kim
;
Heon Sang Jeong
;
Seong Su Nah
;
Hanyong Chen
;
Zigang Dong
;
Young Wan Ham
;
Youngsoo Kim
;
Sang-Bae Han
;
Jin Tae Hong
Adobe PDF(1327Kb)
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Submit date：2017/07/24
ABT-263 enhances sorafenib-induced apoptosis associated with Akt activity and the expression of Bax and p21 (CIP1/WAF1) in human cancer cells1
期刊论文
出版物, 3111, 期号: 0, 页码: 1-35
Authors:
Jingru Li
;
Yicheng Chen
;
Jiali Wan
;
Xin Liu
;
Chunrong Yu
;
Wenhua Li
Adobe PDF(1198Kb)
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Submit date：2017/07/24
Abt-263
Sorafenib
Combination Therapy
Cancer
Effects of a new advanced glycation inhibitor, LR-90, on mitigating arterial stiffening and improving arterial elasticity and compliance in a diabetic rat model: aortic impedance analysis
期刊论文
出版物, 3111, 期号: 0, 页码: 1-33
Authors:
S, Satheesan
;
J L, Figarola
;
T, Dabbs
;
S, Rahbar
;
R, Ermel
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Submit date：2017/07/24
Increased Catalytic Efficiency following Gene Fusion of Bifunctional MethionineSulfoxide Reductase Enzymes from Shewanella oneidensis
期刊论文
Biochemistry, 3111, 页码: 1—9
Authors:
C.B. Li
;
D.M. Zhang
;
S. Ge
;
D.Y. Hong
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Submit date：2017/07/19
Boron in plants: deficiency and toxicity
期刊论文
出版物, 3111, 期号: 0, 页码: 1—24
Authors:
Juan J. Camacho-Cristóbal
;
Jesús Rexach
;
Agustín González-Fontes
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Submit date：2017/07/21
Effects of CO-RM2 on LPS-induced vascular cell adhesion molecule-1 expression and leukocyte adhesion in human rheumatoid synovial fibroblasts
期刊论文
出版物, 3111, 期号: 0, 页码: 1-44
Authors:
Pei-Ling Chi
;
Yu-Chen Chuang
;
Yu-Wen Chen
;
Chih-Chung Lin
;
Li-Der Hsiao
;
Chuen-Mao Yang
Adobe PDF(1319Kb)
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Submit date：2017/07/24
Carbon Monoxide
Lipopolysaccharide
Vcam-1
Leukocyte Adhesion
Resveratrol Decrease Fructose-Induced Oxidative Stress Mediated by NADPH Oxidase via an AMPK-Dependent Mechanism1
期刊论文
出版物, 3111, 期号: 0, 页码: 1-44
Authors:
Pei-Wen Cheng, PhD
;
Wen-Yu Ho, MD, PhD
;
Yu-Ting Su, MS
;
Pei-Jung Lu,PhD
;
Bo-Zone Chen MS
;
Wen-Han Cheng, MS
;
Wen-Hsien Lu, MD, MS
;
Gwo-Ching Sun, MD
;
Tung-Chen Yeh, MD
;
Michael Hsiao DVM, PhD
;
Ching-Jiunn Tseng, MD, PhD
Adobe PDF(1555Kb)
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Submit date：2017/07/24
Ampk
Hypertension
Nucleus Tractus Solitarii
Nitrogen Oxides
Resveratrol
Lead toxicity induced phytotoxic effects on mung bean can be relegated by lead tolerant Bacillus subtilis (PbRB3)
期刊论文
CHEMOSPHERE, 2019, 卷号: 234, 页码: 70-80
Authors:
Arif, Muhammad Saleem
;
Yasmeen, Tahira
;
Shahzad, Sher Muhammad
;
Riaz, Muhammad
;
Rizwan, Muhammad
;
Iqbal, Shahid
;
Asif, Muntaha
;
Soliman, Mona H.
;
Ali, Shafaqat
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Submit date：2019/12/20
Metal contamination
Legume
Oxidative stress
Biosorption
Microbial inoculant
A metabolomic perspective of pazopanib-induced acute hepatotoxicity in mice
期刊论文
XENOBIOTICA, 2019, 卷号: 49, 期号: 6, 页码: 655-670
Authors:
Wang, Yi-Kun
;
Yang, Xiao-Nan
;
Liang, Wei-Qing
;
Xiao, Yao
;
Zhao, Qi
;
Xiao, Xue-Rong
;
Gonzalez, Frank J.
;
Li, Fei
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Submit date：2019/07/29
Pazopanib
hepatotoxicity
metabolomics
oxidative stress
ultra-performance liquid chromatography-electrospray ion source-quadrupole
time-of-flight mass spectrometer (UPLC-ESI-QTOFMS)
Lanostane-type triterpenoids from the fruiting bodies of Ganoderma applanatum
期刊论文
PHYTOCHEMISTRY, 2019, 卷号: 157, 页码: 103-110
Authors:
Peng, XingRong
;
Li, Lei
;
Dong, JinRun
;
Lu, ShuangYang
;
Lu, Jing
;
Li, XiaoNian
;
Zhou, Lin
;
Qiu, MingHua
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Submit date：2019/01/22
Ganoderma applanatum (Pers.) Pat.
Ganodermataceae
Lanostane-type triterpenoid
X-ray crystallographic analyses
Anti-liver fibrosis activity
