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中国科学院昆明植物研究所知识管理系统
Knowledge Management System of Kunming Institute of Botany,CAS
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张雁云 [2]
葛佳 [2]
张宪智 [1]
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GST, P < 0.05) were exhibited by this species. The SAMOVA revealed seven diverging groups of related chlorotypes, six of them had distinct nonoverlapping geographical ranges: one in the northeast comprising 10 populations, a second with a southeast distribution comprising 22 populations, and the remaning four groups comprising 15 populations located in the west part of the species’ range along different river valleys. The genetic clustering of populations into three regions was also supported by analysis of molecular variance, which showed that most genetic variation (82.43%) was found among these three regions. Two clusters were distinguished by both phylogenetic analysis and genealogical analysis of chlorotypes, one consisting of chlorotypes from the western region and the second consisting of those from the eastern region. Significant genetic differences between the two regions might be attributed to vicariance and restricted gene flow, and this vicariance could be explained by the physical environmental heterogeneity on each side of the Tanaka-Kaiyong Line. Following the uplift of the Tibetan Plateau, the reorganization of the major river drainages was primarily caused by river separation and capture events. These historical events could change the distribution of S. davidii from fragmented to continuous (Upper/Lower Jinshajiang and Yalongjiang/Daduhe), and from continuous to fragmented (Nujiang and Jinshajiang/Honghe). However, spatial and temporal patterns of phylogeographic divergence are strongly associated with historical disjunction rather than modern drainage connections. Moreover, the following north-south split in the eastern region and effective isolation with their genetic diversity were essentially modelled by genetic drift. The higher chlorotype richness and genetic divergence for populations in western region compared with other two regions suggests that there were multipe refugia or in situ survival of S. davidii in the Himalayan-Hengduan Mountain region. Fixation of chlorotypes in the northeastern region and near fixation in the southeastern region suggest a recent colonization of these areas. We further found that this species underwent past range expansion around 37-303 thousand years ago (kya). The southeastern populations likely experienced a demographic expansion via unidirectional gene flow along rivers, while northeastern populations underwent a more northward expansion, both from initial populations (s) (21, 22, 23) preserved on eastern refugia (Jinshajiang). This process might have been accompanied with a series of founder effects or bottlenecks making populations genetically impoverished. 3. Phylogeographic analysisbased on nuclear sequence，We sequenced the nuclear (ncpGS) region in all populations sampled, recovering 23 nuclear haplotypes. Compared to cpDNA, both NST (0.470) and GST (0.338) were relatively lower, but NST was also significantly larger than GST. 37.10% of the total variation was distributed among regions which was much lower than that shown by chlorotypes. Thus, more extensive distribution of nuclear haplotypes was exhibited across the geographical range instead of the strong population subdivision observed in chlorotypes. Similarly to the chloroplast data, we found that genetic differentiation of nDNA was positively correlated with the geographical distance, but the increase in the geographical distance between populations did not increase the genetic differentiation of nDNA as rapidly as that of cpDNA. These contrasting levels between the chloroplast and nuclear genomes of S. davidii are likely due to limited gene flow of cpDNA by seeds vs. the extensive gene flow of nDNA by wind-mediated pollen in the population history. We also determined from nuclear markers that haplotype diversity was reduced in the southeastern and northeastern regions due to the loss of rare haplotypes in western region. This reduction of gene diversity is also a signature of founder events or recent bottleneck during post-glacial colonization. However, nuclear diversity within populations remains high. This provides evidence that regionally pollen flow might be sufficiently high to blur the genetic identity of founder populations over a reasonably large spatial scale.3. Relationships among three varieties，The phylogenetic analysis identified two phylogroups of chlorotypes, corresponding to S. davidii var. davidii and var. chuansinesis. The former was distinguished by the abscence of predonminant nuclear haplotype H1 of the latter. The monophyletic group of chlorotypes in var. davidii and var. liangshanesis showed their relatively close relationship. And their genetic divergence from the third variety appears to be relative to their slight morphological difference in leaf size and the divergent environmental niche spaces they occupy. Thus, the observed differences in morphological characters between var. chuansinesis and other two varieties can be explained by the seed dispersal limitation illustrated above (as inferred by geographical separation) and by environmental heterogeneity (as inferred by precipitation or elevation) or by a combination of both. After all, the geological changes, drainage reorganization, and floristic differences following the Himalayan uplift have been suggested to affect the genetic structure of S. davidii. These results provide new insights into the phylogeographic pattern of plants in China. In addition, the unique population genetic structure found in S. davidii has provided important insights into the evolutionary history of this species. The genetic profile uncovered in this study is also critical for its conservation management. Our study has uncovered the existence of at least two ‘evolutionary significant units’ independent units within S. davidii, corresponding to var. davidii from eastern region and var. chuansinensis from western region. The conservation efforts should first focus on most western populations and on the southeastern ones exhibiting high levels of genetic diversity, while the genetically homogeneous northeastern populations located in the degraded Loess Plateau should require much greater conservation 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专题：昆明植物所硕博研究生毕业学位论文
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箭竹属的 DNA 条形码研究
学位论文
: 中国科学院大学, 2022
作者:
吕时雨
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提交时间：2024/05/14
箭竹属， 浅层测序， DNA条形码， 叶绿体基因组， 核糖体DNA
Fargesia, genome-skimming, DNA barcoding, plastome, ribosomal DNA
极小种群野生植物显脉木兰的保护遗传学研究
学位论文
: 中国科学院大学, 2022
作者:
杨丰懋
Adobe PDF(2114Kb)
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提交时间：2024/05/14
保护遗传学，简化基因组，种群历史动态，极小种群野生植物，显脉木兰
Conservation genetics, RAD-seq, Demographic history, Plant Species with Extremely Small Populations, Magnolia fistulosa
山地香茶菜的化学成分及其生物活性研究
学位论文
: 中国科学院大学, 2022
作者:
邱彩玲
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浏览/下载：5/0
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提交时间：2024/05/14
山地香茶菜，二萜，细胞毒，促进溶酶体功能
Isodon oresbius, Diterpenoids, Cytotoxicity, Promotion of lysosomal function
高山流石滩两种伪装紫堇的表型变异与群体遗传结构
学位论文
: 中国科学院大学, 2022
作者:
郭泽敏
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提交时间：2024/05/14
伪装植物
Plant camouflage
表型多样性
Phenotypic diversity
多态性
Polymorphism
自然选择
Natural selection
高山冰缘带
Alpine subnival zone
中国-喜马拉雅地区鳞毛蕨属物种分化格局和成因的研究
学位论文
: 中国科学院大学, 2022
作者:
左政裕
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提交时间：2024/05/14
系统发育基因组学，无融合生殖，杂交，晚中新世，南亚季风
Phylogenomics, Apomixis, Hybridization, Late Miocene, South Asia monsoon
小凉山彝族传统利用植物民族植物学研究
学位论文
: 中国科学院大学, 2022
作者:
阿迪燚旺
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提交时间：2024/05/14
民族植物学， 有用植物， 小凉山彝族，传统知识，民间植物命名
Ethnobotany, Useful Plants, Xiaoliangshan Yi People, Traditional knowledge, Folk Botanical Nomenclature
中国西南山地种子植物多样性演化历史的初步研究
学位论文
: 中国科学院大学, 2022
作者:
杨丹
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提交时间：2024/05/14
生命之树，中国西南山地，演化历史，分化时间估计，多样化速率
Tree of Life, Mountains of Southwest China, Evolutionary History, Divergence Time Estimation, Diversification Rate
滇西北三种垫状植物土壤微生物空间异质性分布的研究
学位论文
, 2021
作者:
肖筑伟
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浏览/下载：21/0
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提交时间：2024/03/20
墨兰（Cymbidium sinense）对氮供应量的生理及生长响应
学位论文
, 2021
作者:
李志雄
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浏览/下载：37/0
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提交时间：2024/03/20
青藏高原 葱属（ 石蒜科）基因组大小进化研究
学位论文
, 2021
作者:
周宁
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浏览/下载：13/0
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提交时间：2024/03/20
