Structural characterization and anticoagulant analysis of the novel branched fucosylated glycosaminoglycan from sea cucumber Holothuria nobilis

doi:10.1016/j.carbpol.2021.118290

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	Structural characterization and anticoagulant analysis of the novel branched fucosylated glycosaminoglycan from sea cucumber Holothuria nobilis
	Li,Shanni ; Zhong,Wei ; Pan,Ying ; Lin,Lisha ; Cai,Ying ; Mao,Hui ; Zhang,Taocui ; Li,Sujuan ; Chen,Ru ; Zhou,Lutan ; Wang,Weili ; Cui,Qinghua ; Yin,Ronghua ; Huang,Shengxiong; Zhao,Jinhua
	2021
发表期刊	CARBOHYDRATE POLYMERS
ISSN	0144-8617
卷号	269 页码:118290
摘要	Glycosaminoglycan HnFG was extracted from sea cucumber Holothuria nobilis. Its chemical structure was characterized by analyzing the physicochemical properties, oligosaccharides from its mild acid hydrolysates and depolymerized products. The disaccharide D-GalNAc4S6S-alpha 1,2-L-Fuc3S-ol found in its mild acid hydrolysates provided a clue for the presence of a unique disaccharide-branch in HnFG. Furthermore, it was confirmed by a series of oligosaccharides from the low-molecular weight HnFG prepared by beta-eliminative depolymerization. Combining with the analysis of its peroxide depolymerized products, the precise structure of HnFG was determined: A chondroitin sulfate E (CS-E)-like backbone branched with sulfated monofucoses (-67%) and disaccharides D-GalNAcS-alpha 1,2-L-Fuc3S (-33%) at O-3 position of each GlcUA. This is the first report on the novel branches in glycosaminoglycan. Biologically, the native and depolymerized HnFG showed potent activities in prolonging the activated partial thrombin time (APTT) and inhibiting intrinsic coagulation Xase (iXase), whereas the oligosaccharides (degree of polymerization <= 6) had no obvious effects.
关键词	Disaccharide branch Fucosylated glycosaminoglycan Holothuria nobilis Oligosaccharide Anticoagulant INTRINSIC TENASE COMPLEX CHONDROITIN SULFATE ACAUDINA-MOLPADIOIDEA FACTOR XASE HEPARIN DEPOLYMERIZATION OLIGOSACCHARIDES POLYSACCHARIDES DERIVATIVES
DOI	10.1016/j.carbpol.2021.118290
WOS记录号	WOS:000678038500006
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/73735
专题	中国科学院昆明植物研究所
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China 2.South Cent Univ Nat, Sch Pharmaceut Sci, Wuhan 430074, Peoples R China 3.Yunnan Univ, Sch Life Sci, Kunming 650500, Yunnan, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Guangxi Inst Tradit Med & Pharmaceut Sci, Nanning 530022, Peoples R China
推荐引用方式 GB/T 7714	Li,Shanni,Zhong,Wei,Pan,Ying,et al. Structural characterization and anticoagulant analysis of the novel branched fucosylated glycosaminoglycan from sea cucumber Holothuria nobilis[J]. CARBOHYDRATE POLYMERS,2021,269:118290.
APA	Li,Shanni.,Zhong,Wei.,Pan,Ying.,Lin,Lisha.,Cai,Ying.,...&Zhao,Jinhua.(2021).Structural characterization and anticoagulant analysis of the novel branched fucosylated glycosaminoglycan from sea cucumber Holothuria nobilis.CARBOHYDRATE POLYMERS,269,118290.
MLA	Li,Shanni,et al."Structural characterization and anticoagulant analysis of the novel branched fucosylated glycosaminoglycan from sea cucumber Holothuria nobilis".CARBOHYDRATE POLYMERS 269(2021):118290.

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