| Attenuation of Pseudomonas aeruginosa biofilm by hordenine: a combinatorial study with aminoglycoside antibiotics | |
| Zhou, Jin-Wei1,2; Hou, Bo3,4; Liu, Gen-Yan5; Jiang, Huan1; Sun, Bing1; Wang, Zhen-Nan1; Shi, Ruo-Fu1; Xu, Yuan6; Wang, Rong2; Jia, Ai-Qun2 | |
| 2018-11-01 | |
| Source Publication | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
 |
| ISSN | 0175-7598 |
| Volume | 102Issue:22Pages:9745-9758 |
| Abstract | Pseudomonas aeruginosa is a ubiquitous pathogen that is the leading cause of chronic infections. Bacterial biofilm formation facilitates CF development and restricts the anti-bacterial potential of many current antibiotics. The capacity of P. aeruginosa to form biofilms and resist antibiotics is closely correlated with quorum sensing (QS). Disrupting QS by QS inhibitors is a promising strategy for treating chronic infections. Here, we evaluated the effect of hordenine, a recently characterized QS inhibitor, on the susceptibility of aminoglycoside antibiotics against P. aeruginosa biofilms. Hordenine significantly enhanced the susceptibility of aminoglycoside antibiotics tobramycin, gentamycin, and amikacin against P. aeruginosa PAO1 biofilm formation. Combinations of hordenine and aminoglycoside antibiotics showed potent efficiency in disrupting the preformed biofilms of P. aeruginosa. Microscopic observations showed flat, scattered, and unstructured biofilm architecture after treatment with hordenine. Mechanistic study further revealed that hordenine treatment led to the downregulation of genes involved in QS and biofilm formation. Thus, our results suggest that hordenine has the potential to function as an antibiotic accelerant in treating P. aeruginosa infections. |
| Keyword | Pseudomonas aeruginosa Biofilm Quorum sensing Susceptibility Antibiotics |
| Language | 英语 |
| Document Type | 期刊论文 |
| Identifier | http://ir.kib.ac.cn/handle/151853/63094 |
| Collection | 植物化学与西部植物资源持续利用国家重点实验室 |
| Corresponding Author | Wang, Rong; Jia, Ai-Qun |
| Affiliation | 1.Nanjing Univ Sci & Technol, Sch Environm & Biol Engn, Nanjing 210094, Jiangsu, Peoples R China 2.Hainan Univ, Key Lab Trop Biol Resources, Minist Educ, State Key Lab Marine Resource Utilizat South Chin, Haikou 570228, Hainan, Peoples R China 3.Chinese Acad Sci, State Key Lab Phytochem & Plant Resources West Ch, Kunming Inst Bot, Kunming 650201, Yunnan, Peoples R China 4.Chinese Acad Sci, Yunnan Key Lab Nat Med Chem, Kunming Inst Bot, Kunming 650201, Yunnan, Peoples R China 5.Nanjing Med Univ, Dept Lab Med, Affiliated Hosp 1, Nanjing 210029, Jiangsu, Peoples R China 6.Nanjing Univ Chinese Med, Sch Med & Life Sci, 138 Xianlin Rd, Nanjing 210023, Jiangsu, Peoples R China |
| Recommended Citation GB/T 7714 | Zhou, Jin-Wei,Hou, Bo,Liu, Gen-Yan,et al. Attenuation of Pseudomonas aeruginosa biofilm by hordenine: a combinatorial study with aminoglycoside antibiotics[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2018,102(22):9745-9758. |
| APA | Zhou, Jin-Wei.,Hou, Bo.,Liu, Gen-Yan.,Jiang, Huan.,Sun, Bing.,...&Jia, Ai-Qun.(2018).Attenuation of Pseudomonas aeruginosa biofilm by hordenine: a combinatorial study with aminoglycoside antibiotics.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,102(22),9745-9758. |
| MLA | Zhou, Jin-Wei,et al."Attenuation of Pseudomonas aeruginosa biofilm by hordenine: a combinatorial study with aminoglycoside antibiotics".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 102.22(2018):9745-9758. |
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