| Quick identification of apoptosis inducer from Isodon eriocalyx by a drug discovery platform composed of analytical high-speed counter-current chromatography and the fluorescence-based caspase-3 biosensor detection | |
Han, Quan-Bin1,2; Yu, Ting3; Lai, Fanny4; Zhou, Yan4; Feng, Chao3; Wang, Wei-Na5; Fu, Xiao-Hui5; Lau, Clara Bik-San1; Luo, Kathy Q.3; Xu, Hong-Xi4; Sun, Han-Dong ; Fung, Kwok-Pui1; Leung, Ping-Chung1
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| 2010-09-15 | |
| 发表期刊 | TALANTA
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| ISSN | 0039-9140 |
| 卷号 | 82期号:4页码:1521-1527 |
| 摘要 | Analytical high-speed counter-current chromatography (HSCCC), a unique liquid-to-liquid separation technology, has an inherent capability to provide perfect fractionation for tracking active ingredients of medicinal herbs, in a quick, efficient, and high-recovery manner. A high throughput screening (HTS) method which utilizes a novel biosensor that selectively detects apoptosis based on the fluorescence resonance energy transfer (FRET) technique, was newly established and proved to be very sensitive in detecting apoptosis induced by various known anticancer drugs. The first combination of both advanced techniques formed an efficient platform for drug discovery and succeeded in quickly identifying the most potent apoptotic constituent of a Chinese herb namely Isodon eriocalyx. The system of n-hexane/ethyl acetate/methanol/water was used as the separation solvent. The solvent ratio was first set at 3:5:3:5 to check the water-soluble part of the crude extract, and then 1:1:1:1 was used to isolate the target compounds. The active fraction was tracked and purified continuously using HSCCC which was guided by the apoptosis detection at gradually decreased drug concentrations. As a result, the most potent apoptosis inducer in this herb was discovered by analytical HSCCC equipped with a 16 ml mini-coil column, using less than 50 ml diphase solvent, from about 50 mg active fraction. It was identified as eriocalyxin B, a well-known antitumor natural product, by NMR analysis of the HSCCC purified fraction. (C) 2010 Elsevier B.V. All rights reserved. |
| 关键词 | Analytical High-speed Counter-current Chromatography Caspase-3 Biosensor Isodon Eriocalyx Apoptosis Inducer Eriocalyxin b |
| 学科领域 | Chemistry, Analytical |
| DOI | 10.1016/j.talanta.2010.07.036 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000282480500066 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.kib.ac.cn/handle/151853/4660 |
| 专题 | 植物化学与西部植物资源持续利用国家重点实验室 |
| 作者单位 | 1.Chinese Univ Hong Kong, Inst Chinese Med, State Key Lab Phytochem & Plant Resources W China, Sha Tin, Hong Kong, Peoples R China 2.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochemistry & Plant Resources W, Kunming 650204, Yunnan, Peoples R China 3.Nanyang Technol Univ, Sch Chem & Biomol Engn, Singapore, Singapore 4.Hong Kong Jockey Club Inst Chinese Med, Shatin, Hong Kong, Peoples R China 5.Shanghai Tauto Biotech Co Ltd, Shanghai 200122, Peoples R China |
| 推荐引用方式 GB/T 7714 | Han, Quan-Bin,Yu, Ting,Lai, Fanny,et al. Quick identification of apoptosis inducer from Isodon eriocalyx by a drug discovery platform composed of analytical high-speed counter-current chromatography and the fluorescence-based caspase-3 biosensor detection[J]. TALANTA,2010,82(4):1521-1527. |
| APA | Han, Quan-Bin.,Yu, Ting.,Lai, Fanny.,Zhou, Yan.,Feng, Chao.,...&Leung, Ping-Chung.(2010).Quick identification of apoptosis inducer from Isodon eriocalyx by a drug discovery platform composed of analytical high-speed counter-current chromatography and the fluorescence-based caspase-3 biosensor detection.TALANTA,82(4),1521-1527. |
| MLA | Han, Quan-Bin,et al."Quick identification of apoptosis inducer from Isodon eriocalyx by a drug discovery platform composed of analytical high-speed counter-current chromatography and the fluorescence-based caspase-3 biosensor detection".TALANTA 82.4(2010):1521-1527. |
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