中国科学院昆明植物研究所机构知识库
Advanced  
KIB OpenIR  > 植物化学与西部植物资源持续利用国家重点实验室  > 期刊论文
题名: Quick identification of apoptosis inducer from Isodon eriocalyx by a drug discovery platform composed of analytical high-speed counter-current chromatography and the fluorescence-based caspase-3 biosensor detection
作者: Han, Quan-Bin1, 2; Yu, Ting3; Lai, Fanny4; Zhou, Yan4; Feng, Chao3; Wang, Wei-Na5; Fu, Xiao-Hui5; Lau, Clara Bik-San1; Luo, Kathy Q.3; Xu, Hong-Xi4; null(孙汉董); Fung, Kwok-Pui1; Leung, Ping-Chung1
刊名: TALANTA
关键词: Analytical high-speed counter-current chromatography ; Caspase-3 biosensor ; Isodon eriocalyx ; Apoptosis inducer ; Eriocalyxin B
英文摘要: Analytical high-speed counter-current chromatography (HSCCC), a unique liquid-to-liquid separation technology, has an inherent capability to provide perfect fractionation for tracking active ingredients of medicinal herbs, in a quick, efficient, and high-recovery manner. A high throughput screening (HTS) method which utilizes a novel biosensor that selectively detects apoptosis based on the fluorescence resonance energy transfer (FRET) technique, was newly established and proved to be very sensitive in detecting apoptosis induced by various known anticancer drugs. The first combination of both advanced techniques formed an efficient platform for drug discovery and succeeded in quickly identifying the most potent apoptotic constituent of a Chinese herb namely Isodon eriocalyx. The system of n-hexane/ethyl acetate/methanol/water was used as the separation solvent. The solvent ratio was first set at 3:5:3:5 to check the water-soluble part of the crude extract, and then 1:1:1:1 was used to isolate the target compounds. The active fraction was tracked and purified continuously using HSCCC which was guided by the apoptosis detection at gradually decreased drug concentrations. As a result, the most potent apoptosis inducer in this herb was discovered by analytical HSCCC equipped with a 16 ml mini-coil column, using less than 50 ml diphase solvent, from about 50 mg active fraction. It was identified as eriocalyxin B, a well-known antitumor natural product, by NMR analysis of the HSCCC purified fraction. (C) 2010 Elsevier B.V. All rights reserved.
出版日期: 2010-09-15
卷号: 82, 期号:4, 页码:1521-1527
DOI标识: 10.1016/j.talanta.2010.07.036
语种: 英语
收录类别: SCI
学科分类: Chemistry, Analytical
ISSN号: 0039-9140
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.kib.ac.cn/handle/151853/4660
Appears in Collections:植物化学与西部植物资源持续利用国家重点实验室_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
201202240027.pdf(487KB)----开放获取--View Download

作者单位: 1.Chinese Univ Hong Kong, Inst Chinese Med, State Key Lab Phytochem & Plant Resources W China, Sha Tin, Hong Kong, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochemistry & Plant Resources W, Kunming 650204, Yunnan, Peoples R China
3.Nanyang Technol Univ, Sch Chem & Biomol Engn, Singapore, Singapore
4.Hong Kong Jockey Club Inst Chinese Med, Shatin, Hong Kong, Peoples R China
5.Shanghai Tauto Biotech Co Ltd, Shanghai 200122, Peoples R China

Recommended Citation:
Han, QB; Yu, T; Lai, F; Zhou, Y; Feng, C; Wang, WN; Fu, XH; Lau, CBS; Luo, KQ; Xu, HX; Sun, HD; Fung, KP; Leung, PC.Quick identification of apoptosis inducer from Isodon eriocalyx by a drug discovery platform composed of analytical high-speed counter-current chromatography and the fluorescence-based caspase-3 biosensor detection,TALANTA,2010,82(4):1521-1527
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Han, Quan-Bin]'s Articles
[Yu, Ting]'s Articles
[Lai, Fanny]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Han, Quan-Bin]‘s Articles
[Yu, Ting]‘s Articles
[Lai, Fanny]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: 201202240027.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  中国科学院昆明植物研究所 - Feedback
Powered by CSpace