Quick identification of apoptosis inducer from Isodon eriocalyx by a drug discovery platform composed of analytical high-speed counter-current chromatography and the fluorescence-based caspase-3 biosensor detection
Han, Quan-Bin1,2; Yu, Ting3; Lai, Fanny4; Zhou, Yan4; Feng, Chao3; Wang, Wei-Na5; Fu, Xiao-Hui5; Lau, Clara Bik-San1; Luo, Kathy Q.3; Xu, Hong-Xi4; Sun, Han-Dong; Fung, Kwok-Pui1; Leung, Ping-Chung1
2010-09-15
发表期刊TALANTA
ISSN0039-9140
卷号82期号:4页码:1521-1527
摘要Analytical high-speed counter-current chromatography (HSCCC), a unique liquid-to-liquid separation technology, has an inherent capability to provide perfect fractionation for tracking active ingredients of medicinal herbs, in a quick, efficient, and high-recovery manner. A high throughput screening (HTS) method which utilizes a novel biosensor that selectively detects apoptosis based on the fluorescence resonance energy transfer (FRET) technique, was newly established and proved to be very sensitive in detecting apoptosis induced by various known anticancer drugs. The first combination of both advanced techniques formed an efficient platform for drug discovery and succeeded in quickly identifying the most potent apoptotic constituent of a Chinese herb namely Isodon eriocalyx. The system of n-hexane/ethyl acetate/methanol/water was used as the separation solvent. The solvent ratio was first set at 3:5:3:5 to check the water-soluble part of the crude extract, and then 1:1:1:1 was used to isolate the target compounds. The active fraction was tracked and purified continuously using HSCCC which was guided by the apoptosis detection at gradually decreased drug concentrations. As a result, the most potent apoptosis inducer in this herb was discovered by analytical HSCCC equipped with a 16 ml mini-coil column, using less than 50 ml diphase solvent, from about 50 mg active fraction. It was identified as eriocalyxin B, a well-known antitumor natural product, by NMR analysis of the HSCCC purified fraction. (C) 2010 Elsevier B.V. All rights reserved.
关键词Analytical High-speed Counter-current Chromatography Caspase-3 Biosensor Isodon Eriocalyx Apoptosis Inducer Eriocalyxin b
资助信息Hong Kong Jockey Club Charities Trust
学科领域Chemistry, Analytical
DOI10.1016/j.talanta.2010.07.036
收录类别SCI
语种英语
WOS研究方向Chemistry
WOS类目Chemistry, Analytical
WOS记录号WOS:000282480500066
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被引频次:21[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/4660
专题植物化学与西部植物资源持续利用国家重点实验室
作者单位1.Chinese Univ Hong Kong, Inst Chinese Med, State Key Lab Phytochem & Plant Resources W China, Sha Tin, Hong Kong, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochemistry & Plant Resources W, Kunming 650204, Yunnan, Peoples R China
3.Nanyang Technol Univ, Sch Chem & Biomol Engn, Singapore, Singapore
4.Hong Kong Jockey Club Inst Chinese Med, Shatin, Hong Kong, Peoples R China
5.Shanghai Tauto Biotech Co Ltd, Shanghai 200122, Peoples R China
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Han, Quan-Bin,Yu, Ting,Lai, Fanny,et al. Quick identification of apoptosis inducer from Isodon eriocalyx by a drug discovery platform composed of analytical high-speed counter-current chromatography and the fluorescence-based caspase-3 biosensor detection[J]. TALANTA,2010,82(4):1521-1527.
APA Han, Quan-Bin.,Yu, Ting.,Lai, Fanny.,Zhou, Yan.,Feng, Chao.,...&Leung, Ping-Chung.(2010).Quick identification of apoptosis inducer from Isodon eriocalyx by a drug discovery platform composed of analytical high-speed counter-current chromatography and the fluorescence-based caspase-3 biosensor detection.TALANTA,82(4),1521-1527.
MLA Han, Quan-Bin,et al."Quick identification of apoptosis inducer from Isodon eriocalyx by a drug discovery platform composed of analytical high-speed counter-current chromatography and the fluorescence-based caspase-3 biosensor detection".TALANTA 82.4(2010):1521-1527.
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