| AS160 controls eukaryotic cell cycle and proliferation by regulating the CDK inhibitor p21 | |
Gongpan, Pianchou1,2; Lu, Yanting1,2; Wang, Fang1,2; Xu, Yuhui1,2; Xiong, Wenyong1
| |
| 2016 | |
| 发表期刊 | CELL CYCLE
 |
| 卷号 | 15期号:13页码:1733-1741 |
| 摘要 | AS160 (TBC1D4) has been implicated in multiple biological processes. However, the role and the mechanism of action of AS160 in the regulation of cell proliferation remain unclear. In this study, we demonstrated that AS160 knockdown led to blunted cell proliferation in multiple cell types, including fibroblasts and cancer cells. The results of cell cycle analysis showed that these cells were arrested in the G1 phase. Intriguingly, this inhibition of cell proliferation and the cell cycle arrest caused by AS160 depletion were glucose independent. Moreover, AS160 silencing led to a marked upregulation of the expression of the cyclin-dependent kinase inhibitor p21. Furthermore, whereas AS160 overexpression resulted in p21 downregulation and rescued the arrested cell cycle in AS160-depeleted cells, p21 silencing rescued the inhibited cell cycle and proliferation in the cells. Thus, our results demonstrated that AS160 regulates glucose-independent eukaryotic cell proliferation through p21-dependent control of the cell cycle, and thereby revealed a molecular mechanism of AS160 modulation of cell cycle and proliferation that is of general physiological significance. |
| 关键词 | As160/tbc1d4 Cell Cycle Cell Proliferation G1/s P21 |
| DOI | 10.1080/15384101.2016.1183853 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000380132600015 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.kib.ac.cn/handle/151853/28516 |
| 专题 | 植物化学与西部植物资源持续利用国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming, Yunnan, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gongpan, Pianchou,Lu, Yanting,Wang, Fang,et al. AS160 controls eukaryotic cell cycle and proliferation by regulating the CDK inhibitor p21[J]. CELL CYCLE,2016,15(13):1733-1741. |
| APA | Gongpan, Pianchou,Lu, Yanting,Wang, Fang,Xu, Yuhui,&Xiong, Wenyong.(2016).AS160 controls eukaryotic cell cycle and proliferation by regulating the CDK inhibitor p21.CELL CYCLE,15(13),1733-1741. |
| MLA | Gongpan, Pianchou,et al."AS160 controls eukaryotic cell cycle and proliferation by regulating the CDK inhibitor p21".CELL CYCLE 15.13(2016):1733-1741. |
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| 16.1183853.pdf（1876KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 下载 | |
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