Eriocalyxin B-Induced Apoptosis in Pancreatic Adenocarcinoma Cells Through Thiol-Containing Antioxidant Systems and Downstream Signalling Pathways
Li, L.1,2,3; Yue, G. G. L.2,3; Pu, J. X.4; Sun, H. D.4; Fung, K. P.1,2,3; Leung, P. C.2,3; Han, Q. B.5; Lau, C. B. S.2,3; Leung, P. S.1; Xu, JC (reprint author), Chinese Acad Sci, Kunming Inst Bot, Key Lab Plant Biodivers & Biogeog East Asia, Kunming 650201, Yunnan, Peoples R China.; j.c.xu@cgiar.org; kdhyde3@gmail.com
2014
发表期刊CURRENT MOLECULAR MEDICINE
ISSN1566-5240
卷号14期号:5页码:673-689
产权排序第一
摘要Thiol-containing antioxidant systems play an important role in regulating cellular redox homeostasis. Several anti-cancer agents act by targeting these systems by inducing the production of reactive oxygen species (ROS). Our earlier studies have shown that Eriocalyxin B (EriB), a diterpenoid isolated from Isodon eriocalyx, possesses anti-pancreatic tumour activities in vitro and in vivo. The present study further demonstrated that only thiol-containing antioxidants, N-acetylcysteine (NAC) or dithiothreitol (DTT), inhibited EriB-induced cytotoxicity and apoptosis. EriB suppressed the glutathione and thioredoxin antioxidant systems, thus increasing the intracellular ROS levels and regulating the MAPK, NF kappa B pathways. Treatment with EriB depleted the intracellular thiol-containing proteins in CAPAN-2 cells. In vivo studies also showed that EriB treatment (2.5 mg/kg) reduced the pancreatic tumour weights significantly in nude mice with increased superoxide levels. Taken together, our results shed important new light on the molecular mechanisms of EriB acting as an apoptogenic agent and its therapeutic potential for pancreatic cancer.
关键词Apoptosis Eriocalyxin b Pancreatic Cancer Reactive Oxygen Species Thiols
资助信息Health and Medical Research Fund of Food and Health Bureau, Hong Kong Special Administrative Region [10110021]
学科领域Medicine, Research & Experimental
收录类别SCI
语种英语
WOS研究方向Research & Experimental Medicine
WOS类目Medicine, Research & Experimental
WOS记录号WOS:000338528100009
引用统计
被引频次:16[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/18277
专题植物化学与西部植物资源持续利用国家重点实验室
通讯作者Xu, JC (reprint author), Chinese Acad Sci, Kunming Inst Bot, Key Lab Plant Biodivers & Biogeog East Asia, Kunming 650201, Yunnan, Peoples R China.; j.c.xu@cgiar.org; kdhyde3@gmail.com
作者单位1.Chinese Univ Hong Kong, Sch Biomed Sci, Hong Kong, Hong Kong, Peoples R China
2.Chinese Univ Hong Kong, Inst Chinese Med, Hong Kong, Peoples R China
3.Chinese Univ Hong Kong, State Key Lab Phytochem & Plant Resources West Ch, Hong Kong, Peoples R China
4.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Mengla, Yunnan, Peoples R China
5.Hong Kong Baptist Univ, Sch Chinese Med, Hong Kong, Hong Kong, Peoples R China
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GB/T 7714
Li, L.,Yue, G. G. L.,Pu, J. X.,et al. Eriocalyxin B-Induced Apoptosis in Pancreatic Adenocarcinoma Cells Through Thiol-Containing Antioxidant Systems and Downstream Signalling Pathways[J]. CURRENT MOLECULAR MEDICINE,2014,14(5):673-689.
APA Li, L..,Yue, G. G. L..,Pu, J. X..,Sun, H. D..,Fung, K. P..,...&kdhyde3@gmail.com.(2014).Eriocalyxin B-Induced Apoptosis in Pancreatic Adenocarcinoma Cells Through Thiol-Containing Antioxidant Systems and Downstream Signalling Pathways.CURRENT MOLECULAR MEDICINE,14(5),673-689.
MLA Li, L.,et al."Eriocalyxin B-Induced Apoptosis in Pancreatic Adenocarcinoma Cells Through Thiol-Containing Antioxidant Systems and Downstream Signalling Pathways".CURRENT MOLECULAR MEDICINE 14.5(2014):673-689.
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