Two immunosuppressive compounds from the mushroom Rubinoboletus ballouii using human peripheral blood mononuclear cells by bioactivity-guided fractionation

	Two immunosuppressive compounds from the mushroom Rubinoboletus ballouii using human peripheral blood mononuclear cells by bioactivity-guided fractionation
	Li, Long-Fei 1,2; Chan, Ben Chung-Lap 1,2; Yue, Grace Gar-Lee 1,2; Lau, Clara Bik-San 1,2; Han, Quan-Bin 4; Leung, Ping-Chung 1,2; Liu, Ji-Kai 5; Fung, Kwok-Pui 1,2,3
通讯作者	Fung, KP (reprint author), Chinese Univ Hong Kong, Inst Chinese Med, E205,Sci Ctr East Block, Shatin, Hong Kong, Peoples R China.,jkliu@mail.kib.ac.cn ; kpfung@cuhk.edu.hk
	2013-10-15
发表期刊	PHYTOMEDICINE
ISSN	0944-7113
卷号	20 期号:13 页码:1196-1202
摘要	Rubinoboletus ballouii is an edible mushroom wildly grown in Yunnan province, China. Up till now, little was known about the chemical and biological properties of this mushroom. The aim of this study was to investigate the immunomodulatory effects of the ethanolic extract of Rubinoboletus ballouii and its fractions on human peripheral blood mononuclear cells (PBMCs) using bioactivity-guided fractionation. The crude extract of the fruiting bodies of RB was fractionated by high-speed counter current chromatography (HSCCC). Twelve fractions were obtained and the third fraction (Fraction C) exerted the most potent anti-inflammatory activities in mitogen-activated PBMCs. Further fractionation of fraction C led to the isolation of two single compounds which were elucidated as 1-ribofuranosyl-s-triazin-2(1H)-one and pistillarin, respectively. The results showed that both 1-ribofuranosyl-s-triazin-2(1H)-one and pistillarin exhibited significant immunosuppressive effects on phytohemagglutinin (PHA)-stimulated human PBMCs by inhibiting [methyl-H-3]-thymidine uptake and inflammatory cytokines productions such as tumor necrosis factor (TNF)-alpha, interleukin (IL)-10, interferon (IFN)-gamma and IL-1 beta. Besides, 1-ribofuranosyl-s-triazin-2(1H)-one was-firstly-found in-natural-resources, and pistillarin was also-isolated-from-the-family Boletaceae for the first time. They exhibited great potential in developing as anti-inflammatory reagents. (C) 2013 Elsevier GmbH. All rights reserved.
关键词	Pistillarin 1-ribofuranosyl-s-triazin-2(1h)-one Rubinoboletus Ballouii Bioassay-guided Fractionation Peripheral Blood Mononuclear Cells (Pbmcs)
学科领域	Plant Sciences ; Chemistry, Medicinal ; Integrative & Complementary Medicine ; Pharmacology & Pharmacy
收录类别	SCI
语种	英语
WOS记录号	WOS:000325445400008
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/17277
专题	植物化学与西部植物资源持续利用国家重点实验室
作者单位	1.Chinese Univ Hong Kong, Inst Chinese Med, Shatin, Hong Kong, Peoples R China 2.Chinese Univ Hong Kong, State Key Lab Phytochem & Plant Resources West Ch, Shatin, Hong Kong, Peoples R China 3.Chinese Univ Hong Kong, Sch Biomed Sci, Shatin, Hong Kong, Peoples R China 4.Hong Kong Baptist Univ, Sch Chinese Med, Kowloon, Hong Kong, Peoples R China 5.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Peoples R China
推荐引用方式 GB/T 7714	Li, Long-Fei,Chan, Ben Chung-Lap,Yue, Grace Gar-Lee,et al. Two immunosuppressive compounds from the mushroom Rubinoboletus ballouii using human peripheral blood mononuclear cells by bioactivity-guided fractionation[J]. PHYTOMEDICINE,2013,20(13):1196-1202.
APA	Li, Long-Fei.,Chan, Ben Chung-Lap.,Yue, Grace Gar-Lee.,Lau, Clara Bik-San.,Han, Quan-Bin.,...&Fung, Kwok-Pui.(2013).Two immunosuppressive compounds from the mushroom Rubinoboletus ballouii using human peripheral blood mononuclear cells by bioactivity-guided fractionation.PHYTOMEDICINE,20(13),1196-1202.
MLA	Li, Long-Fei,et al."Two immunosuppressive compounds from the mushroom Rubinoboletus ballouii using human peripheral blood mononuclear cells by bioactivity-guided fractionation".PHYTOMEDICINE 20.13(2013):1196-1202.

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