Two immunosuppressive compounds from the mushroom Rubinoboletus ballouii using human peripheral blood mononuclear cells by bioactivity-guided fractionation
Li, Long-Fei1,2; Chan, Ben Chung-Lap1,2; Yue, Grace Gar-Lee1,2; Lau, Clara Bik-San1,2; Han, Quan-Bin4; Leung, Ping-Chung1,2; Liu, Ji-Kai5; Fung, Kwok-Pui1,2,3
2013-10-15
发表期刊PHYTOMEDICINE
ISSN0944-7113
卷号20期号:13页码:1196-1202
摘要Rubinoboletus ballouii is an edible mushroom wildly grown in Yunnan province, China. Up till now, little was known about the chemical and biological properties of this mushroom. The aim of this study was to investigate the immunomodulatory effects of the ethanolic extract of Rubinoboletus ballouii and its fractions on human peripheral blood mononuclear cells (PBMCs) using bioactivity-guided fractionation. The crude extract of the fruiting bodies of RB was fractionated by high-speed counter current chromatography (HSCCC). Twelve fractions were obtained and the third fraction (Fraction C) exerted the most potent anti-inflammatory activities in mitogen-activated PBMCs. Further fractionation of fraction C led to the isolation of two single compounds which were elucidated as 1-ribofuranosyl-s-triazin-2(1H)-one and pistillarin, respectively. The results showed that both 1-ribofuranosyl-s-triazin-2(1H)-one and pistillarin exhibited significant immunosuppressive effects on phytohemagglutinin (PHA)-stimulated human PBMCs by inhibiting [methyl-H-3]-thymidine uptake and inflammatory cytokines productions such as tumor necrosis factor (TNF)-alpha, interleukin (IL)-10, interferon (IFN)-gamma and IL-1 beta. Besides, 1-ribofuranosyl-s-triazin-2(1H)-one was-firstly-found in-natural-resources, and pistillarin was also-isolated-from-the-family Boletaceae for the first time. They exhibited great potential in developing as anti-inflammatory reagents. (C) 2013 Elsevier GmbH. All rights reserved.
关键词Pistillarin 1-ribofuranosyl-s-triazin-2(1h)-one Rubinoboletus Ballouii Bioassay-guided Fractionation Peripheral Blood Mononuclear Cells (Pbmcs)
资助信息C.C. Wu Cultural & Education Foundation Fund; The Open Fund of the State Key Laboratory of Phytochemistry and Plant Resources in West China [P2009-KF03]
学科领域Plant Sciences ; Chemistry, Medicinal ; Integrative & Complementary Medicine ; Pharmacology & Pharmacy
收录类别SCI
语种英语
WOS研究方向Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine
WOS类目Plant Sciences ; Chemistry, Medicinal ; Integrative & Complementary Medicine ; Pharmacology & Pharmacy
WOS记录号WOS:000325445400008
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/17277
专题植物化学与西部植物资源持续利用国家重点实验室
作者单位1.Chinese Univ Hong Kong, Inst Chinese Med, Shatin, Hong Kong, Peoples R China
2.Chinese Univ Hong Kong, State Key Lab Phytochem & Plant Resources West Ch, Shatin, Hong Kong, Peoples R China
3.Chinese Univ Hong Kong, Sch Biomed Sci, Shatin, Hong Kong, Peoples R China
4.Hong Kong Baptist Univ, Sch Chinese Med, Kowloon, Hong Kong, Peoples R China
5.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Peoples R China
推荐引用方式
GB/T 7714
Li, Long-Fei,Chan, Ben Chung-Lap,Yue, Grace Gar-Lee,et al. Two immunosuppressive compounds from the mushroom Rubinoboletus ballouii using human peripheral blood mononuclear cells by bioactivity-guided fractionation[J]. PHYTOMEDICINE,2013,20(13):1196-1202.
APA Li, Long-Fei.,Chan, Ben Chung-Lap.,Yue, Grace Gar-Lee.,Lau, Clara Bik-San.,Han, Quan-Bin.,...&Fung, Kwok-Pui.(2013).Two immunosuppressive compounds from the mushroom Rubinoboletus ballouii using human peripheral blood mononuclear cells by bioactivity-guided fractionation.PHYTOMEDICINE,20(13),1196-1202.
MLA Li, Long-Fei,et al."Two immunosuppressive compounds from the mushroom Rubinoboletus ballouii using human peripheral blood mononuclear cells by bioactivity-guided fractionation".PHYTOMEDICINE 20.13(2013):1196-1202.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Li-2013-Two immunosu(1903KB) 开放获取CC BY-NC-SA请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Li, Long-Fei]的文章
[Chan, Ben Chung-Lap]的文章
[Yue, Grace Gar-Lee]的文章
百度学术
百度学术中相似的文章
[Li, Long-Fei]的文章
[Chan, Ben Chung-Lap]的文章
[Yue, Grace Gar-Lee]的文章
必应学术
必应学术中相似的文章
[Li, Long-Fei]的文章
[Chan, Ben Chung-Lap]的文章
[Yue, Grace Gar-Lee]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。