A new anticancer compound, Oblongifolin C, inhibits tumor growth and promotes apoptosis in HeLa cells through bax activation
Feng, Chao2; Zhou, Li-Ying2; Yu, Ting1; Xu, Gang3; Tian, Hong-Lei4; Xu, Jin-Jie2; Xu, Hong-Xi5; Luo, Kathy Qian1
Corresponding AuthorLuo, KQ (reprint author), Nanyang Technol Univ, Div Bioengn, Sch Chem & Biomed Engn, 70 Nanyang Dr, Singapore 637457, Singapore.,xuhongxi88@gmail.com ; kluo@ntu.edu.sg
2012-09-15
Source PublicationINTERNATIONAL JOURNAL OF CANCER
ISSN0020-7136
Volume131Issue:6Pages:1445-1454
AbstractOblongifolin C (OC) was identified as a potent apoptosis inducer from an herbal plant, Garcinia yunnanensis, during our previous bioassay-guided drug screening. In this study, we investigated the signaling pathways through which OC activated apoptosis in HeLa cells. We also compared the IC50 values of OC with that of etoposide, paclitaxel and vinblastine in multiple cancer cell lines including HER2 and P-glycoprotein overexpressing cells. In addition, the in vivo antitumor effect of OC was studied in nude mice model. Our results showed that OC induced a caspase-dependent apoptosis by triggering a series of events in HeLa cells including Bax translocation, cytochrome c release, caspase-3 activation, chromosome fragmentation followed by caspase-8 activation, Bid cleavage and eventually cell death. Addition of a pan-caspase inhibitor or overexpression of an anti-apoptotic protein, Bcl-xL, prevented OC-induced cell death. Moreover, OC exhibited a wide anticancer spectrum in multiple cancer cell lines with comparable IC50 values, regardless of the expression levels of HER2 and P-glycoprotein. In contrast, the IC50 values of three clinical anticancer drugs, etoposide, paclitaxel and vinblastine were significantly elevated in HER2 and/or P-glycoprotein overexpressing cells. Furthermore, OC showed a similar antitumor effect but lower general toxicity than etoposide against xenografted human tumors in nude mice model. All these data suggested that OC is a promising apoptosis inducer with the potential to be developed into a clinical anticancer drug.
KeywordAnticancer Drug Discovery Herbal Medicine Oblongifolin c Apoptosis Bax
DOI10.1002/ijc.27365
Indexed BySCI
Language英语
WOS Research AreaOncology
WOS SubjectOncology
WOS IDWOS:000306735700045
Citation statistics
Cited Times:31[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/15692
Collection植物化学与西部植物资源持续利用国家重点实验室
Affiliation1.Nanyang Technol Univ, Div Bioengn, Sch Chem & Biomed Engn, Singapore 637457, Singapore
2.Hong Kong Univ Sci & Technol, Dept Chem & Biomol Engn, Hong Kong, Hong Kong, Peoples R China
3.Chinese Acad Sci, Kunming Inst Bot, Kunming, Yunnan, Peoples R China
4.Hong Kong Univ Sci & Technol, Dept Biochem, Hong Kong, Hong Kong, Peoples R China
5.Shanghai Univ Tradit Chinese Med, Shanghai, Peoples R China
Recommended Citation
GB/T 7714
Feng, Chao,Zhou, Li-Ying,Yu, Ting,et al. A new anticancer compound, Oblongifolin C, inhibits tumor growth and promotes apoptosis in HeLa cells through bax activation[J]. INTERNATIONAL JOURNAL OF CANCER,2012,131(6):1445-1454.
APA Feng, Chao.,Zhou, Li-Ying.,Yu, Ting.,Xu, Gang.,Tian, Hong-Lei.,...&Luo, Kathy Qian.(2012).A new anticancer compound, Oblongifolin C, inhibits tumor growth and promotes apoptosis in HeLa cells through bax activation.INTERNATIONAL JOURNAL OF CANCER,131(6),1445-1454.
MLA Feng, Chao,et al."A new anticancer compound, Oblongifolin C, inhibits tumor growth and promotes apoptosis in HeLa cells through bax activation".INTERNATIONAL JOURNAL OF CANCER 131.6(2012):1445-1454.
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