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中国科学院昆明植物研究所知识管理系统
Knowledge Management System of Kunming Institute of Botany,CAS
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0.05) between wild (AR = 4.651), semi-cultivated (AR = 5.091) and cultivated (AR = 5.132) populations of C. taliensis, which suggested that the genetic background of long-lived woody plant was not easy to be changed, and there were moderate high gene flow between populations. However, there was a significant difference (P < 0.05) between wild (AR = 5.9) and cultivated (AR = 7.1) populations distributed in the same place in Yun county, Yunnan province, which may result from the hybridization and introgression of species in the tea garden and anthropogenic damages to the wild population. The hypothesis of hybrid origin of C. grandibracteata was tested by morphological and microsatellites analyses. Compared with other species, the locules in ovary of C. grandibracteata are variable, which showed a morphological intermediate and mosaic. Except one private allele, Ninety-nine percent alleles of C. grandibracteata were shared with these of C. taliensis and C. sinensis var. assamica. And C. grandibracteata was nested in the cluster of C. taliensis in the UPGMA tree. Conclusively, our results supported the hypothesis of hybrid origin of C. grandibracteata partly. The speciation of C. grandibracteata was derived from hybridization and asymmetrical introgression potentially. It is possible that C. taliensis was one of its parents, but it still needs more evidences to prove that C. sinensis var. assamica was another parent.","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&sort_by=2&isNonaffiliated=false&search_type=-1&query1=isolation%2Band%2Bidentification&order=desc&&fq=dc.project.title_filter%3ACamellia%5C+taliensis%5C+%5C%28W.%5C+W.%5C+Smith%5C%29%5C+Melchior%2C%5C+a%5C+member%5C+of%5C+Camellia%5C+sect.%5C+Thea%2C%5C+is%5C+an%5C+indigenous%5C+species%5C+in%5C+local%5C+natural%5C+forest%5C+and%5C+has%5C+a%5C+long%5C+cultivative%5C+history%5C+in%5C+western%5C+Yunnan%5C+and%5C+its%5C+neighborhood%2C%5C+where%5C+the%5C+domestications%5C+of%5C+this%5C+species%5C+in%5C+different%5C+historical%5C+periods%5C+and%5C+in%5C+different%5C+ways%5C+can%5C+be%5C+found.%5C+C.%5C+taliensis%5C+is%5C+an%5C+important%5C+contributor%5C+to%5C+the%5C+formations%5C+of%5C+tea%5C+landraces%5C+by%5C+hybridization%5C+and%5C+introgression.%5C+In%5C+the%5C+present%5C+study%2C%5C+14%5C+microsatellite%5C+loci%5C+screened%5C+from%5C+37%5C+loci%5C+were%5C+used%5C+to%5C+explore%5C+the%5C+genetic%5C+diversity%5C+about%5C+this%5C+species%5C+with%5C+579%5C+samples%5C+from%5C+25%5C+populations%5C+%5C%2816%5C+wild%5C+populations%2C%5C+4%5C+semi%5C-cultivated%5C+populations%5C+and%5C+5%5C+cultivated%5C+populations%5C%29.%5C+At%5C+the%5C+same%5C+time%2C%5C+the%5C+potential%5C+hybrid%5C+speciation%5C+of%5C+C.%5C+grandibracteata%2C%5C+was%5C+investigated%5C+using%5C+39%5C+individuals%5C+from%5C+2%5C+populations%2C%5C+along%5C+with%5C+C.%5C+taliensis%5C+and%5C+C.%5C+sinensis%5C+var.%5C+assamica%5C+%5C%2883%5C+individuals%5C+from%5C+4%5C+populations%5C%29%5C+by%5C+the%5C+same%5C+microsatellite%5C+markers.%5C+C.%5C+taliensis%5C+had%5C+a%5C+moderate%5C+high%5C+level%5C+of%5C+genetic%5C+diversity%5C+%5C%28A%5C+%3D%5C+14.3%2C%5C+Ne%3D%5C+5.7%2C%5C+HE%5C+%3D%5C+0.666%2C%5C+I%5C+%3D%5C+1.753%2C%5C+AR%5C+%3D%5C+7.2%2C%5C+PPB%5C+%3D%5C+100%25%5C%29.%5C+This%5C+may%5C+result%5C+from%5C+several%5C+factors%5C+including%5C+K%5C-strategy%2C%5C+genetic%5C+background%2C%5C+gene%5C+flow%5C+between%5C+populations%2C%5C+hybridization%5C+and%5C+introgression%5C+among%5C+species.%5C+Between%5C+wild%5C+populations%5C+of%5C+C.%5C+taliensis%2C%5C+the%5C+gene%5C+flow%5C+was%5C+moderate%5C+high%5C+%5C%28Nm%5C+%3D%5C+1.197%5C%29%2C%5C+and%5C+genetic%5C+variation%5C+was%5C+less%5C+than%5C+20%25%5C+%5C%28GST%5C+%3D%5C+0.147%2C%5C+FST%5C+%3D%5C+0.173%5C%29%2C%5C+which%5C+was%5C+similar%5C+to%5C+other%5C+research%5C+results%5C+of%5C+long%5C-lived%5C+woody%5C+plants%2C%5C+and%5C+reflected%5C+the%5C+genetic%5C+structure%5C+of%5C+its%5C+ancestry%5C+to%5C+same%5C+extent.%5C+There%5C+was%5C+a%5C+high%5C+significant%5C+correlation%5C+between%5C+geographic%5C+distance%5C+and%5C+Nei%E2%80%99s%5C+genetic%5C+distance%5C+%5C%28r%5C+%3D%5C+0.372%2C%5C+P%5C+%3D%5C+0.001%5C%29%5C+of%5C+populations%2C%5C+which%5C+accorded%5C+with%5C+isolation%5C+by%5C+distance%5C+model.%5C+Inferring%5C+from%5C+Bayesian%5C+clustering%5C+of%5C+genotypes%2C%5C+all%5C+individuals%5C+of%5C+C.%5C+taliensis%5C+were%5C+divided%5C+into%5C+two%5C+groups%2C%5C+conflicting%5C+with%5C+the%5C+result%5C+based%5C+on%5C+Nei%E2%80%99s%5C+genetic%5C+distance%5C+and%5C+real%5C+geographic%5C+distribution%2C%5C+which%5C+suggested%5C+there%5C+were%5C+heavy%5C+and%5C+non%5C-random%5C+influences%5C+by%5C+human%5C+practices.%5C+According%5C+to%5C+allelic%5C+richness%2C%5C+there%5C+were%5C+no%5C+significant%5C+differences%5C+%5C%28P%5C+%3E%5C+0.05%5C%29%5C+between%5C+wild%5C+%5C%28AR%5C+%3D%5C+4.651%5C%29%2C%5C+semi%5C-cultivated%5C+%5C%28AR%5C+%3D%5C+5.091%5C%29%5C+and%5C+cultivated%5C+%5C%28AR%5C+%3D%5C+5.132%5C%29%5C+populations%5C+of%5C+C.%5C+taliensis%2C%5C+which%5C+suggested%5C+that%5C+the%5C+genetic%5C+background%5C+of%5C+long%5C-lived%5C+woody%5C+plant%5C+was%5C+not%5C+easy%5C+to%5C+be%5C+changed%2C%5C+and%5C+there%5C+were%5C+moderate%5C+high%5C+gene%5C+flow%5C+between%5C+populations.%5C+However%2C%5C+there%5C+was%5C+a%5C+significant%5C+difference%5C+%5C%28P%5C+%3C%5C+0.05%5C%29%5C+between%5C+wild%5C+%5C%28AR%5C+%3D%5C+5.9%5C%29%5C+and%5C+cultivated%5C+%5C%28AR%5C+%3D%5C+7.1%5C%29%5C+populations%5C+distributed%5C+in%5C+the%5C+same%5C+place%5C+in%5C+Yun%5C+county%2C%5C+Yunnan%5C+province%2C%5C+which%5C+may%5C+result%5C+from%5C+the%5C+hybridization%5C+and%5C+introgression%5C+of%5C+species%5C+in%5C+the%5C+tea%5C+garden%5C+and%5C+anthropogenic%5C+damages%5C+to%5C+the%5C+wild%5C+population.%5C+The%5C+hypothesis%5C+of%5C+hybrid%5C+origin%5C+of%5C+C.%5C+grandibracteata%5C+was%5C+tested%5C+by%5C+morphological%5C+and%5C+microsatellites%5C+analyses.%5C+Compared%5C+with%5C+other%5C+species%2C%5C+the%5C+locules%5C+in%5C+ovary%5C+of%5C+C.%5C+grandibracteata%5C+are%5C+variable%2C%5C+which%5C+showed%5C+a%5C+morphological%5C+intermediate%5C+and%5C+mosaic.%5C+Except%5C+one%5C+private%5C+allele%2C%5C+Ninety%5C-nine%5C+percent%5C+alleles%5C+of%5C+C.%5C+grandibracteata%5C+were%5C+shared%5C+with%5C+these%5C+of%5C+C.%5C+taliensis%5C+and%5C+C.%5C+sinensis%5C+var.%5C+assamica.%5C+And%5C+C.%5C+grandibracteata%5C+was%5C+nested%5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investigation of twelve macrofungi and one lichen including Coriolopsis gallica, Conocybe siliginea, Albatrellus confluens, Scutellinia ascoboloides, Lactarius deliciosus, Thelephora terrestris, Collybia acervata, Shiraia bambusicola, Cortinarius alboviolaceus, Mycena galericulata, Polyporus elegans, Trogia sp., and Sulcaria virens were comprehensively reported in this dissertation.113 different compounds have been isolated and elucidated by varied chromatographic methods and extensive spectroscopic analysis, among which 26 compounds were new ones. The classes of these new compounds include acetylenic acids, tremulane-type sesquiterpenes, cleistanthane-type diterpenes, isocoumarin, norleucine-type non-protein amino acid, etc. The highlight of this dissertation is the phytochemical investigation of Trogia sp. leading to two norleucine-type non-protein amino acids, 2R-amino-4S-hydroxy-5-hexynoic acid (104) and 2R-amino-5-hexynoic acid (105), which are responsible for the toxicity of this fungus. Cellulose microcrystalline column chromatography with n-BuOH-EtOH-AcOH-H2O (4:1:1:2, v/v/v) eluting system was extensively used for isolation of amino acids in this study. Comparing to traditional n-BuOH-AcOH-H2O (BAW) solvent system, a time-saving and efficient TLC solvent system OWF (organin layer-water-formic acid) for amino acids detection was developed. TLC solvent system OWF was prepared as below: solvent OW was composed of CHCl3-CH3COCH3-MeOH (10:4:4, v/v/v), which was saturated with water; 1 to 4 drops of formic acid was added to 1 ml solvent OW when used.In the last chapter, the chemical, biological and mycological literature dealing with the isolation, structure elucidation, biological activities, and synthesis of pigments produced by those fungi that produce conspicuous fruiting bodies (macromycetes) was reviewed. 198 references between June 2003 to December 2009 are cited.","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&sort_by=2&isNonaffiliated=false&search_type=-1&query1=isolation%2Band%2Bidentification&order=desc&&fq=dc.project.title_filter%3AChemical%5C+investigation%5C+of%5C+twelve%5C+macrofungi%5C+and%5C+one%5C+lichen%5C+including%5C+Coriolopsis%5C+gallica%2C%5C+Conocybe%5C+siliginea%2C%5C+Albatrellus%5C+confluens%2C%5C+Scutellinia%5C+ascoboloides%2C%5C+Lactarius%5C+deliciosus%2C%5C+Thelephora%5C+terrestris%2C%5C+Collybia%5C+acervata%2C%5C+Shiraia%5C+bambusicola%2C%5C+Cortinarius%5C+alboviolaceus%2C%5C+Mycena%5C+galericulata%2C%5C+Polyporus%5C+elegans%2C%5C+Trogia%5C+sp.%2C%5C+and%5C+Sulcaria%5C+virens%5C+were%5C+comprehensively%5C+reported%5C+in%5C+this%5C+dissertation.113%5C+different%5C+compounds%5C+have%5C+been%5C+isolated%5C+and%5C+elucidated%5C+by%5C+varied%5C+chromatographic%5C+methods%5C+and%5C+extensive%5C+spectroscopic%5C+analysis%2C%5C+among%5C+which%5C+26%5C+compounds%5C+were%5C+new%5C+ones.%5C+The%5C+classes%5C+of%5C+these%5C+new%5C+compounds%5C+include%5C+acetylenic%5C+acids%2C%5C+tremulane%5C-type%5C+sesquiterpenes%2C%5C+cleistanthane%5C-type%5C+diterpenes%2C%5C+isocoumarin%2C%5C+norleucine%5C-type%5C+non%5C-protein%5C+amino%5C+acid%2C%5C+etc.%5C+The%5C+highlight%5C+of%5C+this%5C+dissertation%5C+is%5C+the%5C+phytochemical%5C+investigation%5C+of%5C+Trogia%5C+sp.%5C+leading%5C+to%5C+two%5C+norleucine%5C-type%5C+non%5C-protein%5C+amino%5C+acids%2C%5C+2R%5C-amino%5C-4S%5C-hydroxy%5C-5%5C-hexynoic%5C+acid%5C+%5C%28104%5C%29%5C+and%5C+2R%5C-amino%5C-5%5C-hexynoic%5C+acid%5C+%5C%28105%5C%29%2C%5C+which%5C+are%5C+responsible%5C+for%5C+the%5C+toxicity%5C+of%5C+this%5C+fungus.%5C+Cellulose%5C+microcrystalline%5C+column%5C+chromatography%5C+with%5C+n%5C-BuOH%5C-EtOH%5C-AcOH%5C-H2O%5C+%5C%284%5C%3A1%5C%3A1%5C%3A2%2C%5C+v%5C%2Fv%5C%2Fv%5C%29%5C+eluting%5C+system%5C+was%5C+extensively%5C+used%5C+for%5C+isolation%5C+of%5C+amino%5C+acids%5C+in%5C+this%5C+study.%5C+Comparing%5C+to%5C+traditional%5C+n%5C-BuOH%5C-AcOH%5C-H2O%5C+%5C%28BAW%5C%29%5C+solvent%5C+system%2C%5C+a%5C+time%5C-saving%5C+and%5C+efficient%5C+TLC%5C+solvent%5C+system%5C+OWF%5C+%5C%28organin%5C+layer%5C-water%5C-formic%5C+acid%5C%29%5C+for%5C+amino%5C+acids%5C+detection%5C+was%5C+developed.%5C+TLC%5C+solvent%5C+system%5C+OWF%5C+was%5C+prepared%5C+as%5C+below%5C%3A%5C+solvent%5C+OW%5C+was%5C+composed%5C+of%5C+CHCl3%5C-CH3COCH3%5C-MeOH%5C+%5C%2810%5C%3A4%5C%3A4%2C%5C+v%5C%2Fv%5C%2Fv%5C%29%2C%5C+which%5C+was%5C+saturated%5C+with%5C+water%5C%3B%5C+1%5C+to%5C+4%5C+drops%5C+of%5C+formic%5C+acid%5C+was%5C+added%5C+to%5C+1%5C+ml%5C+solvent%5C+OW%5C+when%5C+used.In%5C+the%5C+last%5C+chapter%2C%5C+the%5C+chemical%2C%5C+biological%5C+and%5C+mycological%5C+literature%5C+dealing%5C+with%5C+the%5C+isolation%2C%5C+structure%5C+elucidation%2C%5C+biological%5C+activities%2C%5C+and%5C+synthesis%5C+of%5C+pigments%5C+produced%5C+by%5C+those%5C+fungi%5C+that%5C+produce%5C+conspicuous%5C+fruiting%5C+bodies%5C+%5C%28macromycetes%5C%29%5C+was%5C+reviewed.%5C+198%5C+references%5C+between%5C+June%5C+2003%5C+to%5C+December%5C+2009%5C+are%5C+cited."},{"jsname":"lastIndexed","jscount":"2025-06-04"}],"资助项目","dc.project.title_filter")'>
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Oxytocin inhibits the activity of acid-sensing ion channels through the vasopressin-1a receptor in primary sensory neurons1
期刊论文
出版物, 3111, 期号: 0, 页码: 1-34
作者:
Fang Qiu
;
Chun-Yu Qiu
;
Huilan Cai
;
Ting-Ting Liu
;
Zu-Wei Qu
;
Zhifan Yang
;
Jia-Da Li
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Qun-Yong Zhou
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Wang-Ping Hu
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提交时间：2017/07/24
Oxytocin
Acid-sensing Ion Channel
Proton-gated Current
Vasopressin-1a Receptor
Dorsal Root Ganglion Neuron
Electrophysiology
Pain
紫花碎米荠的化学成分研究
期刊论文
出版物, 3111, 期号: 0, 页码: 1—7
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紫花碎米荠
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化学成分
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黄酮
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The novel benzimidazole derivative BRP-7 inhibits leukotriene biosynthesis in vitro and in vivo by targeting 5-lipoxygenase-activating protein (FLAP)1
期刊论文
出版物, 3111, 期号: 0, 页码: 1-42
作者:
C Pergola
;
J Gerstmeier
;
B Monch
;
B Çaliskan
;
S Luderer
;
C Weinige
;
D Barz
;
J Maczewsky
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S Pace
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A Rossi
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L Sautebin
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E Banoglu
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O Werz
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Structural and mechanistic basis of penicillin-bindingprotein inhibition by lactivicins
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NATURE CHEMICAL BIOLOGY, 3111, 期号: 0, 页码: 1—5
作者:
Pauline Macheboeuf
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Delphine S Fischer
;
Tom Brown Jr
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Astrid Zervosen
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Andre´Luxen
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Bernard Joris
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Andrea Dessen
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Christopher J Schofield
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提交时间：2017/07/21
The formation and function of plant volatiles: perfumes for pollinator attraction and defense
期刊论文
Physiology and metabolism, 3111, 期号: 0, 页码: 237-243
作者:
Eran Pichersky
;
Jonathan Gershenzon
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提交时间：2017/07/26
Boron in plants: deficiency and toxicity
期刊论文
出版物, 3111, 期号: 0, 页码: 1—24
作者:
Juan J. Camacho-Cristóbal
;
Jesús Rexach
;
Agustín González-Fontes
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提交时间：2017/07/21
Effects of CO-RM2 on LPS-induced vascular cell adhesion molecule-1 expression and leukocyte adhesion in human rheumatoid synovial fibroblasts
期刊论文
出版物, 3111, 期号: 0, 页码: 1-44
作者:
Pei-Ling Chi
;
Yu-Chen Chuang
;
Yu-Wen Chen
;
Chih-Chung Lin
;
Li-Der Hsiao
;
Chuen-Mao Yang
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提交时间：2017/07/24
Carbon Monoxide
Lipopolysaccharide
Vcam-1
Leukocyte Adhesion
New Phenolic Dimers from Plant Paeonia suffruticosa and Their Cytotoxicity and NO Production Inhibition
期刊论文
Molecules, 2023
作者:
Qianqian Meng
;
Shunyao Tong
;
Yuqing Zhao
;
Xingrong Peng
;
Zhenghui Li
;
Tao Feng
;
Jikai Liu
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浏览/下载：731/151
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提交时间：2023/10/07
UPLC-Q-Orbitrap-MS/MS-Guided Isolation of Bioactive Withanolides from the Fruits of Physalis angulata
期刊论文
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2023, 卷号: 71, 期号: 44, 页码: 16581-16592
作者:
Wang,Peng
;
Yang,Xiao-Meng
;
Hu,Zhan-Xing
;
Li,Ya-Nan
;
Yang,Jue
;
Hao,Xiao-Jiang
;
Yuan,Chun-Mao
;
Yi,Ping
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提交时间：2024/07/10
Physalis angulata
rare withanolidederivatives
isolation and identification
anticancer
anti-inflammatory activity
CYTOTOXIC WITHANOLIDES
CONSTITUENTS
PLANTS
L.
Leucosceptrane Sesterterpenoids as a New Type of Natural Immunosuppressive Agents in Treating Sepsis
期刊论文
JOURNAL OF MEDICINAL CHEMISTRY, 2023, 卷号: 67, 期号: 1, 页码: 513-528
作者:
Guo,Kai
;
Zhou,Ting-Ting
;
Luo,Shi-Hong
;
Liu,Yan-Chun
;
Liu,Yan
;
Li,Sheng-Hong
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提交时间：2024/07/10
GLANDULAR TRICHOMES
PRODUCTS
HARBOR
CANUM
