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中国科学院昆明植物研究所知识管理系统
Knowledge Management System of Kunming Institute of Botany,CAS
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许建初 [56]
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0.05) between wild (AR = 4.651), semi-cultivated (AR = 5.091) and cultivated (AR = 5.132) populations of C. taliensis, which suggested that the genetic background of long-lived woody plant was not easy to be changed, and there were moderate high gene flow between populations. However, there was a significant difference (P < 0.05) between wild (AR = 5.9) and cultivated (AR = 7.1) populations distributed in the same place in Yun county, Yunnan province, which may result from the hybridization and introgression of species in the tea garden and anthropogenic damages to the wild population. The hypothesis of hybrid origin of C. grandibracteata was tested by morphological and microsatellites analyses. Compared with other species, the locules in ovary of C. grandibracteata are variable, which showed a morphological intermediate and mosaic. Except one private allele, Ninety-nine percent alleles of C. grandibracteata were shared with these of C. taliensis and C. sinensis var. assamica. And C. grandibracteata was nested in the cluster of C. taliensis in the UPGMA tree. Conclusively, our results supported the hypothesis of hybrid origin of C. grandibracteata partly. The speciation of C. grandibracteata was derived from hybridization and asymmetrical introgression potentially. It is possible that C. taliensis was one of its parents, but it still needs more evidences to prove that C. sinensis var. assamica was another 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Postdoctoral Science Foundation","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&sort_by=2&isNonaffiliated=false&search_type=-1&query1=SINENSIS&order=desc&&fq=dc.project.title_filter%3AChina%5C+Postdoctoral%5C+Science%5C+Foundation"},{"jsname":"China Postdoctoral Science Foundation[Y71B283261]","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&sort_by=2&isNonaffiliated=false&search_type=-1&query1=SINENSIS&order=desc&&fq=dc.project.title_filter%3AChina%5C+Postdoctoral%5C+Science%5C+Foundation%5C%5BY71B283261%5C%5D"},{"jsname":"Chinese Academy of Sciences[2013T2S0030]","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&sort_by=2&isNonaffiliated=false&search_type=-1&query1=SINENSIS&order=desc&&fq=dc.project.title_filter%3AChinese%5C+Academy%5C+of%5C+Sciences%5C%5B2013T2S0030%5C%5D"},{"jsname":"Chinese Academy of Sciences[2013T2S003]","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&sort_by=2&isNonaffiliated=false&search_type=-1&query1=SINENSIS&order=desc&&fq=dc.project.title_filter%3AChinese%5C+Academy%5C+of%5C+Sciences%5C%5B2013T2S003%5C%5D"},{"jsname":"Chinese Academy of Sciences[XDA19050000]","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&sort_by=2&isNonaffiliated=false&search_type=-1&query1=SINENSIS&order=desc&&fq=dc.project.title_filter%3AChinese%5C+Academy%5C+of%5C+Sciences%5C%5BXDA19050000%5C%5D"},{"jsname":"Chinese Academy of Sciences[XDB31000000]","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&sort_by=2&isNonaffiliated=false&search_type=-1&query1=SINENSIS&order=desc&&fq=dc.project.title_filter%3AChinese%5C+Academy%5C+of%5C+Sciences%5C%5BXDB31000000%5C%5D"},{"jsname":"Conservation and Application of National Strategic Tropical Plant Resources: Theory and Practice[2017XTBG-F05]","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&sort_by=2&isNonaffiliated=false&search_type=-1&query1=SINENSIS&order=desc&&fq=dc.project.title_filter%3AConservation%5C+and%5C+Application%5C+of%5C+National%5C+Strategic%5C+Tropical%5C+Plant%5C+Resources%5C%3A%5C+Theory%5C+and%5C+Practice%5C%5B2017XTBG%5C-F05%5C%5D"},{"jsname":"Craigia yunnanensis W. W. Smith & W. E. Evans (Tiliaceae) is an endangered deciduous tree species which has high scientific and economic value. C. yunnanensis is seriously threatened and has been pushed to the verge of extinction due to vegetation destruction in China and consequent contraction of its distribution. Hence, it was listed as a nationally rare and endangered plant in 1999 and has also been proposed as a second-ranked plant for national protection in China and included in IUCN red list. As a scientifically important and valued tree species with endangered status, the wild populations of C. yunnanensis therefore represent is a genetic resource that must be conserved. To provide basic information for its conservation, the population dynamics and population size structures, pollination biology and breeding system, eleven fitness-related characters and the genetic variability based on AFLP were comprehensively studied. The main results are summarized as follows: A total of six wild populations of C. yunnanensis were found in two disjunct regions of Yunnan, i.e. WenShan (SE Yunnan) and DeHong (SW Yunnan), from 2005 to 2007. Additionally, in all but one of the populations we detected, mature trees were felled between 2005 and 2007, so destruction of most of these populations is ongoing. Across the six populations of extant C. yunnanensis found during our study, the total number of mature (reproductive) individuals detected was 584 in 2007，plus larger numbers of seedling and resprouts from cut trunks. The result of surveying Population structure showed that there are two regeneration types which are seedlings and sprouts. Seedlings occurred abundantly in gaps or open areas and the size class frequency distributions were often discontinuous, and the same general pattern occurred in all the investigated populations for juveniles and adults. The numbers of seed-origin individuals did however decline sharply with increasing size, indicating a high mortality rate going from seedling to sapling stage may be a problem for this species. Additionally, the cash crop cultivation and logging seriously threaten the survival of the species. We conducted field observations and artificial pollination experiments on the floral biology, pollination process and breeding system of Craigia yunnanensis in Fadou, Xichou county of Yunnan province. The lifespan of a single hermaphrodite flower is approximately 3-4 days. A cyme has 2-9 flowered. The flowering period of an inflorescence is usually 5-14 days. The flowers of C. yunnanensis were protandrous. The stamens were within petal-like staminodes in the opening flowers until the flower withered. Without touchment, the bractlike staminodes can’t open. Self-pollination was partially avoided by temporal and spatial isolation of male and female organs within the same flower. However, autogamous and geitonogamous pollination is unavoidable because of the large number of flowers on a single tree and the action of pollinators. The values of both OCI (≥4) and P/O (1381) and the results of bagging tests indicated there was no apomixes in C. yunnanensis and the breeding system of the species was outcrossing with partial self-compatibility and the pollinators were required during the pollination process. The most frequent effective floral visitor was only beautiful fly (Chrysomyia megacephala). Fruit set and seed set in natural condition were 56.67±3.85％ and 6.26±0.75％, respectively. Therefore, lack of pollinators, low pollination efficiency, unavoidable geitonogamous pollination and partial self-compatibility and inbreeding in small populations may account for the low fruit set, especially seed set.Variations in seed traits, seed germination, and seedling growth characters among six Craigia yunnanensis populations were evaluated. All seed and seedling traits exhibited significant differences among populations (P < 0.05). The fitness of seed as assessed by seed size, seed germination and seedling trait was independent of population size, except for the number of seeds per capsule (r = 0.93，P < 0.01). Correlations between geo-climatic variables of seed origin and seed and seedling related characters were insignificant (P > 0.05). For some populations, germination capacity in 12-h photoperiod was significantly higher than that in completed darkness（W-FD: P < 0.01, W-JD: P < 0.05）.Genetic variation within and among six populations was assessed using AFLP markers. Genetic diversity was higher at species level (PPL = 69.19%, HE = 0.221) than at population level (PPL = 26.22%, HE = 0.095, Is =0.140), and populations in southeast Yunnan were strongly differentiated from those in southwest Yunnan (Nei’s GST = 0.575; FST = 0.655). UPGMA analysis demonstrated a clear genetic division between the two populations from DeHong (SW Yunnan; D-JD and D-HG) and the four from WenShan (SE Yunnan; W-FD, W-LH, W-ML, and W-MG). Within-population genetic variation was significantly correlated with population isolation (r(PPL) = -0.94, P = 0.006; r(HE) = -0.85, P = 0.032; r(Is) = -0.87, P = 0.025), but not with population size (r(PPL) = 0.63, P = 0.178; r(HE) = 0.54, P = 0.268; r(Is) = 0.56, P = 0.249).","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&sort_by=2&isNonaffiliated=false&search_type=-1&query1=SINENSIS&order=desc&&fq=dc.project.title_filter%3ACraigia%5C+yunnanensis%5C+W.%5C+W.%5C+Smith%5C+%5C%26%5C+W.%5C+E.%5C+Evans%5C+%5C%28Tiliaceae%5C%29%5C+is%5C+an%5C+endangered%5C+deciduous%5C+tree%5C+species%5C+which%5C+has%5C+high%5C+scientific%5C+and%5C+economic%5C+value.%5C+C.%5C+yunnanensis%5C+is%5C+seriously%5C+threatened%5C+and%5C+has%5C+been%5C+pushed%5C+to%5C+the%5C+verge%5C+of%5C+extinction%5C+due%5C+to%5C+vegetation%5C+destruction%5C+in%5C+China%5C+and%5C+consequent%5C+contraction%5C+of%5C+its%5C+distribution.%5C+Hence%2C%5C+it%5C+was%5C+listed%5C+as%5C+a%5C+nationally%5C+rare%5C+and%5C+endangered%5C+plant%5C+in%5C+1999%5C+and%5C+has%5C+also%5C+been%5C+proposed%5C+as%5C+a%5C+second%5C-ranked%5C+plant%5C+for%5C+national%5C+protection%5C+in%5C+China%5C+and%5C+included%5C+in%5C+IUCN%5C+red%5C+list.%5C+As%5C+a%5C+scientifically%5C+important%5C+and%5C+valued%5C+tree%5C+species%5C+with%5C+endangered%5C+status%2C%5C+the%5C+wild%5C+populations%5C+of%5C+C.%5C+yunnanensis%5C+therefore%5C+represent%5C+is%5C+a%5C+genetic%5C+resource%5C+that%5C+must%5C+be%5C+conserved.%5C+To%5C+provide%5C+basic%5C+information%5C+for%5C+its%5C+conservation%2C%5C+the%5C+population%5C+dynamics%5C+and%5C+population%5C+size%5C+structures%2C%5C+pollination%5C+biology%5C+and%5C+breeding%5C+system%2C%5C+eleven%5C+fitness%5C-related%5C+characters%5C+and%5C+the%5C+genetic%5C+variability%5C+based%5C+on%5C+AFLP%5C+were%5C+comprehensively%5C+studied.%5C+The%5C+main%5C+results%5C+are%5C+summarized%5C+as%5C+follows%5C%3A%5C+A%5C+total%5C+of%5C+six%5C+wild%5C+populations%5C+of%5C+C.%5C+yunnanensis%5C+were%5C+found%5C+in%5C+two%5C+disjunct%5C+regions%5C+of%5C+Yunnan%2C%5C+i.e.%5C+WenShan%5C+%5C%28SE%5C+Yunnan%5C%29%5C+and%5C+DeHong%5C+%5C%28SW%5C+Yunnan%5C%29%2C%5C+from%5C+2005%5C+to%5C+2007.%5C+Additionally%2C%5C+in%5C+all%5C+but%5C+one%5C+of%5C+the%5C+populations%5C+we%5C+detected%2C%5C+mature%5C+trees%5C+were%5C+felled%5C+between%5C+2005%5C+and%5C+2007%2C%5C+so%5C+destruction%5C+of%5C+most%5C+of%5C+these%5C+populations%5C+is%5C+ongoing.%5C+Across%5C+the%5C+six%5C+populations%5C+of%5C+extant%5C+C.%5C+yunnanensis%5C+found%5C+during%5C+our%5C+study%2C%5C+the%5C+total%5C+number%5C+of%5C+mature%5C+%5C%28reproductive%5C%29%5C+individuals%5C+detected%5C+was%5C+584%5C+in%5C+2007%EF%BC%8Cplus%5C+larger%5C+numbers%5C+of%5C+seedling%5C+and%5C+resprouts%5C+from%5C+cut%5C+trunks.%5C+The%5C+result%5C+of%5C+surveying%5C+Population%5C+structure%5C+showed%5C+that%5C+there%5C+are%5C+two%5C+regeneration%5C+types%5C+which%5C+are%5C+seedlings%5C+and%5C+sprouts.%5C+Seedlings%5C+occurred%5C+abundantly%5C+in%5C+gaps%5C+or%5C+open%5C+areas%5C+and%5C+the%5C+size%5C+class%5C+frequency%5C+distributions%5C+were%5C+often%5C+discontinuous%2C%5C+and%5C+the%5C+same%5C+general%5C+pattern%5C+occurred%5C+in%5C+all%5C+the%5C+investigated%5C+populations%5C+for%5C+juveniles%5C+and%5C+adults.%5C+The%5C+numbers%5C+of%5C+seed%5C-origin%5C+individuals%5C+did%5C+however%5C+decline%5C+sharply%5C+with%5C+increasing%5C+size%2C%5C+indicating%5C+a%5C+high%5C+mortality%5C+rate%5C+going%5C+from%5C+seedling%5C+to%5C+sapling%5C+stage%5C+may%5C+be%5C+a%5C+problem%5C+for%5C+this%5C+species.%5C+Additionally%2C%5C+the%5C+cash%5C+crop%5C+cultivation%5C+and%5C+logging%5C+seriously%5C+threaten%5C+the%5C+survival%5C+of%5C+the%5C+species.%5C+We%5C+conducted%5C+field%5C+observations%5C+and%5C+artificial%5C+pollination%5C+experiments%5C+on%5C+the%5C+floral%5C+biology%2C%5C+pollination%5C+process%5C+and%5C+breeding%5C+system%5C+of%5C+Craigia%5C+yunnanensis%5C+in%5C+Fadou%2C%5C+Xichou%5C+county%5C+of%5C+Yunnan%5C+province.%5C+The%5C+lifespan%5C+of%5C+a%5C+single%5C+hermaphrodite%5C+flower%5C+is%5C+approximately%5C+3%5C-4%5C+days.%5C+A%5C+cyme%5C+has%5C+2%5C-9%5C+flowered.%5C+The%5C+flowering%5C+period%5C+of%5C+an%5C+inflorescence%5C+is%5C+usually%5C+5%5C-14%5C+days.%5C+The%5C+flowers%5C+of%5C+C.%5C+yunnanensis%5C+were%5C+protandrous.%5C+The%5C+stamens%5C+were%5C+within%5C+petal%5C-like%5C+staminodes%5C+in%5C+the%5C+opening%5C+flowers%5C+until%5C+the%5C+flower%5C+withered.%5C+Without%5C+touchment%2C%5C+the%5C+bractlike%5C+staminodes%5C+can%E2%80%99t%5C+open.%5C+Self%5C-pollination%5C+was%5C+partially%5C+avoided%5C+by%5C+temporal%5C+and%5C+spatial%5C+isolation%5C+of%5C+male%5C+and%5C+female%5C+organs%5C+within%5C+the%5C+same%5C+flower.%5C+However%2C%5C+autogamous%5C+and%5C+geitonogamous%5C+pollination%5C+is%5C+unavoidable%5C+because%5C+of%5C+the%5C+large%5C+number%5C+of%5C+flowers%5C+on%5C+a%5C+single%5C+tree%5C+and%5C+the%5C+action%5C+of%5C+pollinators.%5C+The%5C+values%5C+of%5C+both%5C+OCI%5C+%5C%28%E2%89%A54%5C%29%5C+and%5C+P%5C%2FO%5C+%5C%281381%5C%29%5C+and%5C+the%5C+results%5C+of%5C+bagging%5C+tests%5C+indicated%5C+there%5C+was%5C+no%5C+apomixes%5C+in%5C+C.%5C+yunnanensis%5C+and%5C+the%5C+breeding%5C+system%5C+of%5C+the%5C+species%5C+was%5C+outcrossing%5C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micholitzii complex is composed of 5 species: C. micholitzii Dyer, C. bifida (Dyer) K. D. Hill,C. longipetiolula D. Y. Wang, C. debaoensis Y. C. Zhong et C J. Chen, C. multipinnata C J. Chen et S. Y. Yang，and distributed from southwest China to central Vietnam and eastern Laos. Based on sequence data from two maternally inherited cpDNA and one biparentally nuclear DNA fragments, our study revealed the population genetic structure of C. micholitzii complex and explored the potential causes. The evolutionary and demographic histories were investigated. The genetic relationship among species in the complex was also clarified.The results were summarized as follows: 1. Phylogeographic analysis based on chloroplast sequences，We examined chloroplast sequence variation of the atpB-rbcLand psbA-trnHintergenic spacers in 27 populations of C. micholitzii complex, recovering 26 haplotypes. The average within-population diversity (HS = 0.140) was low while total diversity (HT = 0.911) was high. Population differentiation was also high(GST = 0.846, NST = 0.919), indicating significant phylogeographical structure (NST > GST,p < 0.001) and low levels of seed-based gene flow. C. debaoensis (Cycadaceae) is an endangered species restricted to the border of Guangxi and Yunnan province in southwest China. This species has been classified into two types: sand and karst, according to the soil matrix they grow on. We examined chloroplast sequence variation of the cpDNA sequences from 11 populations of this species. Significant population genetic differentiation was detected (GST= 0.684 and FST = 0.74160). There was marked genetic differentiation between populations in the sand and karst regions and no expansion was detected. Climate changes during glacial periods have had significant effects on the current distribution of cycads. The molecular phylogenetic data, together with the geographic distribution of the haplotypes, suggest that C. debaoensis experienced range contraction during glacial periods, and that the current populations are still confined to the original refugia in southwest China which have favorable habitats in glacial period. These results imply that small refugia were maintained in both sand and karst regions during the LGM (last glacial maximum). This species had no postglacial recolonization and only stayed in these refugia up to now. The low within-population diversity of C. debaoensis suggests that there were strong bottleneck events or founder effects within each separate region during the Quaternary climatic oscillations. Relatively high genetic and haplotype diversities were detected in the newly discovered populations, which located at intermediate locality of sand regions and had morphological variation; this is probably the consequence of the admixture of different haplotypes colonizing the area from separate sources. C. micholitzii occurs in the Annan Highlands in central Vietnam near the Laos border. C. bifida occurs in North Vietnam; its distribution extends across the border into adjacent localities in Guangxi and Yunnan in China. For the comparability between them,theywere considered as the same species C. micholitzii by many academicians. The cpDNA sequences from 11 populations showed that these very controversial species, C. micholitzii and C. bifida, is paraphyletic and should belong to the same species C. micholitzii. AMOVA analysis showed that the component of among-population within region/species (76.46%) was unexpectedly larger than the among-species/region component (14.97%), which also indicates that there is no justification for recognizing two species as C. micholitzii and C. bifida. This hypothesis was also supported by the geological data, especially the neotectonic history of the indo-china block, which started to move south since Oligocene and cause the geographic isolation of these two groups. Therefore, the most likely explanation to the phenotypic similarities between these two groups may be the retention of ancestral polymorphisms in the paraphyletic group due to incomplete lineage sorting. Furthermore, the similarities may also be ascribed to pollen-mediated gene flow among geographically proximate populations and/or phenotypic convergence under similar selection schemes in the same region. C.micholitzi had the higest genetic diversity (HT = 0.980,) and genetic differentiation (GST = 0.830, NST = 0.915) among the C. micholitzii complex. The high genetic diversity might be attributed to its long evolutionary history, highly diverse habitats. The ineffective mode of seed dispersal and dramatic neotectonic movement in the distribution range of this species could result in the high genetic differentiation. 2. Phylogeographic analysis based on nuclear ribosomal sequences, We sequenced the nrDNA ITS in all 27 populations sampled, 7 haplotypes were identified, among which C. micholitzii had 6, while C. multipinnata, C. longipetiolula and C. debaoensis shared the remaining one. Compared to chloroplast genes, nuclear genes had higher correlation between genetic and geographical distance, but lower interspecies differentiation (54.42% vs 25.24%). Phylogeographical structure of C. micholitzii and C.bifida based on ITS Variation was consistent with the morphology differentiation. This similar in nuclear gene should be ascribed to pollen-mediated gene flow among geographically proximate populations.Long-distance gene flow over the two groups was clearly interrupted, which brought on the nrDNA genetic differenciation between the geographically isolated groups, to a certain extent affected the morphological variation. 3. Interspecies relationships among Cycas micholitzii complex, We analysed chloroplast sequence variation of the atpB-rbcL and psbA-trnH intergenic spacers in 27 populations sampled of C. micholitzii complex, AMOVA analysis showed that the component of among-species/region component (59.21%). However, phylogenic analysis showed that the haplotypes of C. micholitzii complex couldn`t grouped into four clusters closely corresponding to the narrowly defined C. micholitzi, C. multipinnata, C. debaoensis and C. longipetiolula. We concluded that the conflict may result from several factors: firstly incomplete lineage sorting of C. micholitzii; secondly hybridization/introgression of sympatrically cycads, which would be supported by evidence base on nrDNA ITS sequences; thirdly intramolecular recombination in cpDNA of cycads; eventually the neotectonic movement in the distribution range of this 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study can reveal important biological features of plants and answers to a certain degree in phylogeny and distribution of genetic materials and so forth. By hard working of cytologists, chromosome data of plants have been increased to a great abundance, but yet disorderly distributed in different magazines, which made researches based on the whole chromosome data of one taxon rarely launched. Scientific databases have become increasingly indispensable as researching data growing daily. As Cytological studies are booming in China, in order to fill the absence of digital and statistical data of plant chromosome researches and chromosome atlas, we started to develop a Chinese Seed Plants Chromosome Database, aiming to construct a database and start to record published chromosome data of Chinese seed plants. Based on this database, we chose the part of gymnosperms and gave a discussion to the features of its chromosomes’ evolution and variation. Cytological experiments have been applied to some important phyto-groups for phylogeny research and germplasm identification.Part I: The Chinese Seed Plants Chromosome Database and Discussion on the features of Gymnosperms chromosomes，1 The Chinese Seed Plants Chromosome Database，The frame of database was constructed by Microsoft Access 2003. 19 items of data were included in, they are: Chinese and Latin names of family, genus and species; plant pictures, mitosis metaphase and karyotype figures; morphological characteristics and distributions of the plant; chromosome numbers and basic numbers; karyotype formula; karyotype description; origin of the plant material; literature and the source of photos. In this database, data can be checked and shared easily by extracted out in species sorted interface or family sorted interface. 120 species in 29 genera and 10 families of Gymnospers have been collected and input to the database. In Angiosperms, 61 species in 10 genera of family Magnoliaceae and 80 species in 3 genera of family Theaceae have been collected and input to the database.2 Discussion on the features of evolution and variation of Gymnosperms chromosomes，By data collection of the database, we analyzed chromosome features of the group Gymnosperm. Plants of Gymnosperm had been through a long historical evolution on earth, fossil records of which originated from the late Devonian period. Once an authoritative and major classification level in the plant kingdom, most Gymnosperms have been extinct unless conifers, cycads, Ginkgo and Getales. Three main features of Gymnosperm chromosomes are: relatively large chromosome, which can be recognized from figures in the database; constant chromosome numbers, in most families of Gymnosperm the basic chromosome number keeps a certain value; comparatively low variation, karyotype under family level differs a little. The variation of chromosomes in Gymnosperm is dominated by Robertsonian changes. Contrary to common variation type in Angiosperms, the variation from high unsymmetric karyotype to low unsymmetric karyotype was found in existence in Gymnosperm.Part II: cytology research on some important phyto-groups，3 Karyomorphology of three species in the order Huerteales and their phylogenetic implications，The karyomorphology of three species in Dipentodon (Dipentodontaceae), Perrottetia (Celastraceae), and Tapiscia (Tapisciaceae), namely Dipentodon sinicus, Perrottetia racemosa, and Tapiscia sinensis, was investigated in the study. Recent molecular research has discovered close relationships among these three genera, which has led to the establishment of the order Huerteales with Perrottetia being placed in Dipentodontaceae. Herein we report the chromosome numbers of D. sinicus and P. racemosa for the first time, and present their karyotype formulas as 2n = 34 = 22sm + 12st (D. sinicus), 2n = 20 = 11m + 9sm (P. racemosa), and 2n = 30 = 22m(2SAT) + 8sm (T. sinensis). Asymmetry of their karyotypes is categorized to be Type 3B in D. sinicus, Type 2A in P. racemosa, and Type 2A in T. sinensis. Each of the species shows special cytological features. Compared with Perrottetia, Dipentodon has a different basic chromosome number, a higher karyotype asymmetry, and different karyomorphology of its interphase nuclei, mitotic prophase, and metaphase. Thus, on the basis of these results, we have reservations regarding the suggestion of placing Dipentodon and Perrottetia together in the family Dipentodontaceae.4 Genomic analyses of intergeneric hybrids between Michelia crassipes and M. calcicola by GISH，Genomic in situ hybridization (GISH) is becoming the method of choice for identifying parental chromosomes in interspecific hybrids. Interspecific F1 hybrid between Michelia crassipes and M. calcicola, tow highly ornamental species in Michelia of Magnolicaceae, has been analized by double-colored GISH with its parents’ genome as the probe. Research gave the results that the chromosome number of the F1 hybrid is 2n=38 as the same of species in Michelia and other genera in Magnoliaceae, the basic chromosome is x=19, the karyotype formula is 2n=38=32m+6sm, and the asymmetry of karyotype is 1B type. Based on chromosome data of Michelia in our database, the karyotype of this genus is featured mostly by metacentric chromosomes and submetacentric chromosomes. In Mechelia, the variation range of submetacentric chromosomes is 4 to 18 and of the karyotype asymmetry is 1A to 2B type. Both the karyotype and karyotype asymmetry type of F1 hybrid is among the variation range of Michelia. The figure of GISH showed that all the 38 chromosomes of F1 hybrid have crossing parental signals, and signal on the no.1 and no.7 chromosome showed differences, which proved that both the parental genome have been transmitted to and recombinated in F1 hybrid.","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&sort_by=2&isNonaffiliated=false&search_type=-1&query1=SINENSIS&order=desc&&fq=dc.project.title_filter%3ACytology%5C+study%5C+can%5C+reveal%5C+important%5C+biological%5C+features%5C+of%5C+plants%5C+and%5C+answers%5C+to%5C+a%5C+certain%5C+degree%5C+in%5C+phylogeny%5C+and%5C+distribution%5C+of%5C+genetic%5C+materials%5C+and%5C+so%5C+forth.%5C+By%5C+hard%5C+working%5C+of%5C+cytologists%2C%5C+chromosome%5C+data%5C+of%5C+plants%5C+have%5C+been%5C+increased%5C+to%5C+a%5C+great%5C+abundance%2C%5C+but%5C+yet%5C+disorderly%5C+distributed%5C+in%5C+different%5C+magazines%2C%5C+which%5C+made%5C+researches%5C+based%5C+on%5C+the%5C+whole%5C+chromosome%5C+data%5C+of%5C+one%5C+taxon%5C+rarely%5C+launched.%5C+Scientific%5C+databases%5C+have%5C+become%5C+increasingly%5C+indispensable%5C+as%5C+researching%5C+data%5C+growing%5C+daily.%5C+As%5C+Cytological%5C+studies%5C+are%5C+booming%5C+in%5C+China%2C%5C+in%5C+order%5C+to%5C+fill%5C+the%5C+absence%5C+of%5C+digital%5C+and%5C+statistical%5C+data%5C+of%5C+plant%5C+chromosome%5C+researches%5C+and%5C+chromosome%5C+atlas%2C%5C+we%5C+started%5C+to%5C+develop%5C+a%5C+Chinese%5C+Seed%5C+Plants%5C+Chromosome%5C+Database%2C%5C+aiming%5C+to%5C+construct%5C+a%5C+database%5C+and%5C+start%5C+to%5C+record%5C+published%5C+chromosome%5C+data%5C+of%5C+Chinese%5C+seed%5C+plants.%5C+Based%5C+on%5C+this%5C+database%2C%5C+we%5C+chose%5C+the%5C+part%5C+of%5C+gymnosperms%5C+and%5C+gave%5C+a%5C+discussion%5C+to%5C+the%5C+features%5C+of%5C+its%5C+chromosomes%E2%80%99%5C+evolution%5C+and%5C+variation.%5C+Cytological%5C+experiments%5C+have%5C+been%5C+applied%5C+to%5C+some%5C+important%5C+phyto%5C-groups%5C+for%5C+phylogeny%5C+research%5C+and%5C+germplasm%5C+identification.Part%5C+I%5C%3A%5C+The%5C+Chinese%5C+Seed%5C+Plants%5C+Chromosome%5C+Database%5C+and%5C+Discussion%5C+on%5C+the%5C+features%5C+of%5C+Gymnosperms%5C+chromosomes%EF%BC%8C1%5C+%C2%A0The%5C+Chinese%5C+Seed%5C+Plants%5C+Chromosome%5C+Database%EF%BC%8CThe%5C+frame%5C+of%5C+database%5C+was%5C+constructed%5C+by%5C+Microsoft%5C+Access%5C+2003.%5C+19%5C+items%5C+of%5C+data%5C+were%5C+included%5C+in%2C%5C+they%5C+are%5C%3A%5C+Chinese%5C+and%5C+Latin%5C+names%5C+of%5C+family%2C%5C+genus%5C+and%5C+species%5C%3B%5C+plant%5C+pictures%2C%5C+mitosis%5C+metaphase%5C+and%5C+karyotype%5C+figures%5C%3B%5C+morphological%5C+characteristics%5C+and%5C+distributions%5C+of%5C+the%5C+plant%5C%3B%5C+chromosome%5C+numbers%5C+and%5C+basic%5C+numbers%5C%3B%5C+karyotype%5C+formula%5C%3B%5C+karyotype%5C+description%5C%3B%5C+origin%5C+of%5C+the%5C+plant%5C+material%5C%3B%5C+literature%5C+and%5C+the%5C+source%5C+of%5C+photos.%5C+In%5C+this%5C+database%2C%5C+data%5C+can%5C+be%5C+checked%5C+and%5C+shared%5C+easily%5C+by%5C+extracted%5C+out%5C+in%5C+species%5C+sorted%5C+interface%5C+or%5C+family%5C+sorted%5C+interface.%5C+120%5C+species%5C+in%5C+29%5C+genera%5C+and%5C+10%5C+families%5C+of%5C+Gymnospers%5C+have%5C+been%5C+collected%5C+and%5C+input%5C+to%5C+the%5C+database.%5C+In%5C+Angiosperms%2C%5C+61%5C+species%5C+in%5C+10%5C+genera%5C+of%5C+family%5C+Magnoliaceae%5C+and%5C+80%5C+species%5C+in%5C+3%5C+genera%5C+of%5C+family%5C+Theaceae%5C+have%5C+been%5C+collected%5C+and%5C+input%5C+to%5C+the%5C+database.2%5C+Discussion%5C+on%5C+the%5C+features%5C+of%5C+evolution%5C+and%5C+variation%5C+of%5C+Gymnosperms%5C+chromosomes%EF%BC%8CBy%5C+data%5C+collection%5C+of%5C+the%5C+database%2C%5C+we%5C+analyzed%5C+chromosome%5C+features%5C+of%5C+the%5C+group%5C+Gymnosperm.%5C+Plants%5C+of%5C+Gymnosperm%5C+had%5C+been%5C+through%5C+a%5C+long%5C+historical%5C+evolution%5C+on%5C+earth%2C%5C+fossil%5C+records%5C+of%5C+which%5C+originated%5C+from%5C+the%5C+late%5C+Devonian%5C+period.%5C+Once%5C+an%5C+authoritative%5C+and%5C+major%5C+classification%5C+level%5C+in%5C+the%5C+plant%5C+kingdom%2C%5C+most%5C+Gymnosperms%5C+have%5C+been%5C+extinct%5C+unless%5C+conifers%2C%5C+cycads%2C%5C+Ginkgo%5C+and%5C+Getales.%5C+Three%5C+main%5C+features%5C+of%5C+Gymnosperm%5C+chromosomes%5C+are%5C%3A%5C+relatively%5C+large%5C+chromosome%2C%5C+which%5C+can%5C+be%5C+recognized%5C+from%5C+figures%5C+in%5C+the%5C+database%5C%3B%5C+constant%5C+chromosome%5C+numbers%2C%5C+in%5C+most%5C+families%5C+of%5C+Gymnosperm%5C+the%5C+basic%5C+chromosome%5C+number%5C+keeps%5C+a%5C+certain%5C+value%5C%3B%5C+comparatively%5C+low%5C+variation%2C%5C+karyotype%5C+under%5C+family%5C+level%5C+differs%5C+a%5C+little.%5C+The%5C+variation%5C+of%5C+chromosomes%5C+in%5C+Gymnosperm%5C+is%5C+dominated%5C+by%5C+Robertsonian%5C+changes.%5C+Contrary%5C+to%5C+common%5C+variation%5C+type%5C+in%5C+Angiosperms%2C%5C+the%5C+variation%5C+from%5C+high%5C+unsymmetric%5C+karyotype%5C+to%5C+low%5C+unsymmetric%5C+karyotype%5C+was%5C+found%5C+in%5C+existence%5C+in%5C+Gymnosperm.Part%5C+II%5C%3A%5C+cytology%5C+research%5C+on%5C+some%5C+important%5C+phyto%5C-groups%EF%BC%8C3%5C+Karyomorphology%5C+of%5C+three%5C+species%5C+in%5C+the%5C+order%5C+Huerteales%5C+and%5C+thei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Glabridin inhibits migration and invasion by transcriptional inhibition of matrix metalloproteinase-9 through modulation of NF-B and AP-1 activity in human liver cancer cells lines
期刊论文
出版物, 3111, 期号: 0, 页码: 1—28
Authors:
Ming-Ju Hsieh
;
Chiao-Wen Lin
;
Shun-Fa Yang
;
Mu-Kuan Chen
;
Hui-Ling Chiou
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Glabridin
Migration
Invasion
Matrix Metalloproteinase-9
Hepatoma
云南各民族食用花卉中的人文因素
期刊论文
Ziran Zazhi, 3111, 卷号: 23, 页码: 292-297
Authors:
刘怡涛
;
龙春林
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Submit date：2017/07/19
顽拗性种子种质保存现象
期刊论文
出版物, 3111, 页码: 37-40
Authors:
陆旺金
;
傅家瑞
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Submit date：2017/07/19
Exploring ascomycete diversity in Yunnan, China I: resolving ambiguous taxa in Phaeothecoidiellaceae and investigating conservation implications of fungi
期刊论文
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, 2023, 卷号: 13, 页码: 1252387
Authors:
Hongsanan,Sinang
;
Phookamsak,Rungtiwa
;
Bhat,Darbhe Jayarama
;
Wanasinghe,Dhanushka N.
;
Promputtha,Itthayakorn
;
Suwannarach,Nakarin
;
Sandamali,Diana
;
Lumyong,Saisamorn
;
Xu,Jianchu
;
Xie,Ning
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Submit date：2024/07/10
Dothideomycetes
fungal taxonomy
Mycosphaerellales
novel taxa
sootyblotch/flyspeck fungi
REFINED FAMILIES
SOOTY BLOTCH
SP-NOV.
REPETOPHRAGMA
GENERA
FLYSPECK
CLASSIFICATION
XISHUANGBANNA
BIODIVERSITY
SPORIDESMIUM
Modular and Stereoselective One-Pot Total Synthesis of Icosasaccharide Motif from Cordyceps sinensis EPS-1A Glycan
期刊论文
ORGANIC LETTERS, 2023, 卷号: 25, 期号: 40, 页码: 7364-7368
Authors:
Sun,Xingchun
;
Chen,Zhiyuan
;
Yang,Rui
;
Wang,Mei
;
Wang,Xiufang
;
Zhang,Qi
;
Xiao,Guozhi
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Submit date：2024/07/10
MYCELIAL FERMENTATION
GLYCOSYLATION
POLYSACCHARIDES
The complete chloroplast genome of Pseudotsuga sinensis, a China endemic species
期刊论文
MITOCHONDRIAL DNA PART B-RESOURCES, 2023, 卷号: 8, 期号: 1, 页码: 23-25
Authors:
Li,Wang Jun
;
Feng,Tu
;
Li,Jun
;
He,Bin
;
Zou,Shun
;
Liu,Peng Ju
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Submit date：2024/05/09
Pseudotsuga sinensis
chloroplast genome
phylogeny
Taxonomy, phylogeny, and successful cultivation of Marasmiellus scandens (Basidiomycota) associated with Aquilaria sinensis (agarwood tree) in China
期刊论文
PHYTOTAXA, 2023, 卷号: 616, 期号: 1, 页码: 60-68
Authors:
Zhang,Shiyu
;
Ye,Shuang
;
Tibpromma,Saowaluck
;
Karunarathna,Samantha C.
;
Zhao,Gaojuan
;
Mapook,Ausana
;
Xu,Jianchu
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agarwood-associated fungi
Basidiomycota
endophytic fungi
Guangxi
ITS
mushroom cultivation
new record
ENDOPHYTIC FUNGI
OMPHALOTACEAE
AGARICALES
EUAGARICS
Unravelling the genetic diversity and population structure of common walnut in the Iranian Plateau
期刊论文
BMC PLANT BIOLOGY, 2023, 卷号: 23, 期号: 1, 页码: 201
Authors:
Shavvon,Robabeh Shahi
;
Qi,Hai-Ling
;
Mafakheri,Mohammad
;
Fan,Pen-Zheng
;
Wu,Hong-Yu
;
Vahdati,Fatemeh Bazdid
;
Al-Shmgani,Hanady S.
;
Wang,Yue-Hua
;
Liu,Jie
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Common walnut
Germplasm conservation
Genetic variation
Iranian Plateau
Persian walnut
Population structure
Refugia
JUGLANS-REGIA L.
PERSIAN WALNUT
GENOTYPES
MARKERS
JUGLANDACEAE
VARIABILITY
PHYLOGENY
SINENSIS
PROGRAM
Intra-Specific Variation in Desiccation Tolerance of Citrus sinensis 'bingtangcheng' (L.) Seeds under Different Environmental Conditions in China
期刊论文
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2023, 卷号: 24, 期号: 8, 页码: 7393
Authors:
Chen,Hongying
;
Visscher,Anne M.
;
Ai,Qin
;
Yang,Lan
;
Pritchard,Hugh W.
;
Li,Weiqi
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Submit date：2024/05/09
fruit crop
maternal environment
plant genetic resources
seed storage
sweet orange
HEAT-SHOCK PROTEINS
FRUIT-DEVELOPMENT
EXPRESSION
MATURATION
GERMINATION
GENE
STORABILITY
RESISTANCE
LONGEVITY
NETWORK
Multi-Gene Phylogeny and Taxonomy of the Wood-Rotting Fungal Genus Phlebia sensu lato (Polyporales, Basidiomycota)
期刊论文
JOURNAL OF FUNGI, 2023, 卷号: 9, 期号: 3, 页码: 320
Authors:
Zhao,Changlin
;
Qu,Menghan
;
Huang,Ruoxia
;
Karunarathna,Samantha C.
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Submit date：2024/05/09
biodiversity
degradation
Meruliaceae
molecular systematics
wood-inhabiting fungi
SP. NOV. POLYPORALES
MOLECULAR-IDENTIFICATION
MORPHOLOGICAL CHARACTERS
1ST RECORD
MERULIACEAE
CLASSIFICATION
MUSHROOMS
INFERENCE
CHINA
PHANEROCHAETE
