×
验证码:
换一张
忘记密码？
记住我
×
登录
中文版
|
English
中国科学院昆明植物研究所知识管理系统
Knowledge Management System of Kunming Institute of Botany,CAS
登录
注册
ALL
ORCID
题名
作者
学科领域
关键词
资助项目
文献类型
出处
收录类别
出版者
发表日期
存缴日期
学科门类
学习讨论厅
图片搜索
粘贴图片网址
首页
研究单元&专题
作者
文献类型
学科分类
知识图谱
新闻&公告
在结果中检索
研究单元&专题
昆明植物所硕博研究... [12]
中国科学院东亚植物多... [5]
资源植物与生物技术所... [3]
中国西南野生生物种质... [3]
共享文献 [2]
植物化学与西部植物资... [1]
更多...
作者
李德铢 [3]
胡虹 [2]
张石宝 [2]
孙航 [1]
郭振华 [1]
曾英 [1]
更多...
文献类型
期刊论文 [13]
学位论文 [12]
专著 [2]
发表日期
2010 [27]
语种
中文 [13]
英语 [12]
出处
AMERICAN J... [1]
ANNALS OF ... [1]
BIOTECHNOL... [1]
BOTANICAL ... [1]
GENETICS A... [1]
JOURNAL OF... [1]
更多...
资助项目
GST, P < 0.05) were exhibited by this species. The SAMOVA revealed seven diverging groups of related chlorotypes, six of them had distinct nonoverlapping geographical ranges: one in the northeast comprising 10 populations, a second with a southeast distribution comprising 22 populations, and the remaning four groups comprising 15 populations located in the west part of the species’ range along different river valleys. The genetic clustering of populations into three regions was also supported by analysis of molecular variance, which showed that most genetic variation (82.43%) was found among these three regions. Two clusters were distinguished by both phylogenetic analysis and genealogical analysis of chlorotypes, one consisting of chlorotypes from the western region and the second consisting of those from the eastern region. Significant genetic differences between the two regions might be attributed to vicariance and restricted gene flow, and this vicariance could be explained by the physical environmental heterogeneity on each side of the Tanaka-Kaiyong Line. Following the uplift of the Tibetan Plateau, the reorganization of the major river drainages was primarily caused by river separation and capture events. These historical events could change the distribution of S. davidii from fragmented to continuous (Upper/Lower Jinshajiang and Yalongjiang/Daduhe), and from continuous to fragmented (Nujiang and Jinshajiang/Honghe). However, spatial and temporal patterns of phylogeographic divergence are strongly associated with historical disjunction rather than modern drainage connections. Moreover, the following north-south split in the eastern region and effective isolation with their genetic diversity were essentially modelled by genetic drift. The higher chlorotype richness and genetic divergence for populations in western region compared with other two regions suggests that there were multipe refugia or in situ survival of S. davidii in the Himalayan-Hengduan Mountain region. Fixation of chlorotypes in the northeastern region and near fixation in the southeastern region suggest a recent colonization of these areas. We further found that this species underwent past range expansion around 37-303 thousand years ago (kya). The southeastern populations likely experienced a demographic expansion via unidirectional gene flow along rivers, while northeastern populations underwent a more northward expansion, both from initial populations (s) (21, 22, 23) preserved on eastern refugia (Jinshajiang). This process might have been accompanied with a series of founder effects or bottlenecks making populations genetically impoverished. 3. Phylogeographic analysisbased on nuclear sequence，We sequenced the nuclear (ncpGS) region in all populations sampled, recovering 23 nuclear haplotypes. Compared to cpDNA, both NST (0.470) and GST (0.338) were relatively lower, but NST was also significantly larger than GST. 37.10% of the total variation was distributed among regions which was much lower than that shown by chlorotypes. Thus, more extensive distribution of nuclear haplotypes was exhibited across the geographical range instead of the strong population subdivision observed in chlorotypes. Similarly to the chloroplast data, we found that genetic differentiation of nDNA was positively correlated with the geographical distance, but the increase in the geographical distance between populations did not increase the genetic differentiation of nDNA as rapidly as that of cpDNA. These contrasting levels between the chloroplast and nuclear genomes of S. davidii are likely due to limited gene flow of cpDNA by seeds vs. the extensive gene flow of nDNA by wind-mediated pollen in the population history. We also determined from nuclear markers that haplotype diversity was reduced in the southeastern and northeastern regions due to the loss of rare haplotypes in western region. This reduction of gene diversity is also a signature of founder events or recent bottleneck during post-glacial colonization. However, nuclear diversity within populations remains high. This provides evidence that regionally pollen flow might be sufficiently high to blur the genetic identity of founder populations over a reasonably large spatial scale.3. Relationships among three varieties，The phylogenetic analysis identified two phylogroups of chlorotypes, corresponding to S. davidii var. davidii and var. chuansinesis. The former was distinguished by the abscence of predonminant nuclear haplotype H1 of the latter. The monophyletic group of chlorotypes in var. davidii and var. liangshanesis showed their relatively close relationship. And their genetic divergence from the third variety appears to be relative to their slight morphological difference in leaf size and the divergent environmental niche spaces they occupy. Thus, the observed differences in morphological characters between var. chuansinesis and other two varieties can be explained by the seed dispersal limitation illustrated above (as inferred by geographical separation) and by environmental heterogeneity (as inferred by precipitation or elevation) or by a combination of both. After all, the geological changes, drainage reorganization, and floristic differences following the Himalayan uplift have been suggested to affect the genetic structure of S. davidii. These results provide new insights into the phylogeographic pattern of plants in China. In addition, the unique population genetic structure found in S. davidii has provided important insights into the evolutionary history of this species. The genetic profile uncovered in this study is also critical for its conservation management. Our study has uncovered the existence of at least two ‘evolutionary significant units’ independent units within S. davidii, corresponding to var. davidii from eastern region and var. chuansinensis from western region. The conservation efforts should first focus on most western populations and on the southeastern ones exhibiting high levels of genetic diversity, while the genetically homogeneous northeastern populations located in the degraded Loess Plateau should require much greater conservation efforts.","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&fq=dc.date.issued.year%3A2010&sort_by=2&isNonaffiliated=false&search_type=-1&query1=Clones&order=desc&&fq=dc.project.title_filter%3ASophora%5C+davidii%5C+%5C%28Franch.%5C%29%5C+Skeels%5C+is%5C+an%5C+endemic%5C+species%5C+to%5C+China%2C%5C+and%5C+widely%5C+distributed%5C+in%5C+the%5C+dry%5C+valleys%5C+of%5C+the%5C+Himalayan%5C-Hengduan%5C+Mountain%5C+Systems%2C%5C+the%5C+Yungui%5C+Plateau%2C%5C+the%5C+Qinling%5C+Mountain%2C%5C+the%5C+Loess%5C+Plateau%5C+and%5C+other%5C+places%5C+of%5C+China.%5C+Previous%5C+studies%5C+of%5C+plant%5C+phylogeography%5C+have%5C+focused%5C+mainly%5C+on%5C+some%5C+taxa%5C+from%5C+the%5C+mountainous%5C+areas%5C+of%5C+China%2C%5C+relatively%5C+few%5C+studies%5C+have%5C+been%5C+conducted%5C+on%5C+plant%5C+taxa%5C+from%5C+the%5C+river%5C+valleys.%5C+The%5C+population%5C+dynamics%5C+and%5C+evolutionary%5C+history%5C+of%5C+species%5C+in%5C+such%5C+habitat%5C+remain%5C+less%5C+unknown%2C%5C+including%5C+the%5C+factors%5C+affecting%5C+the%5C+population%5C+genetic%5C+structure%5C+and%5C+its%5C+potential%5C+refugia%5C+in%5C+glaciation.%5C+In%5C+this%5C+study%2C%5C+we%5C+first%5C+determine%5C+the%5C+chromosome%5C+number%2C%5C+ploidy%5C+and%5C+karyotype%5C+of%5C+most%5C+populations%5C+we%5C+sampled.%5C+Then%2C%5C+based%5C+on%5C+sequence%5C+data%5C+from%5C+two%5C+maternally%5C+inherited%5C+cpDNA%5C+and%5C+one%5C+biparentally%5C+inherited%5C+nuclear%5C+DNA%5C+fragments%2C%5C+our%5C+study%5C+revealed%5C+the%5C+genetic%5C+diversity%5C+and%5C+population%5C+genetic%5C+structure%5C+of%5C+S.%5C+davidii%5C+and%5C+factors%5C+affecting%5C+them.%5C+The%5C+demographic%5C+history%5C+and%5C+potential%5C+refugia%5C+of%5C+this%5C+speices%5C+were%5C+investigated%5C+and%5C+the%5C+genetic%5C+relationship%5C+among%5C+three%5C+varieties%5C+was%5C+also%5C+clarified.%5C+The%5C+main%5C+results%5C+are%5C+summarized%5C+as%5C+follows%5C%3A1.%5C+Cytogeography%EF%BC%8CThe%5C+chromosome%5C+number%5C+and%5C+karyotypes%5C+of%5C+14%5C+S.%5C+davidii%5C+populations%5C+have%5C+been%5C+studied.%5C+The%5C+results%5C+showed%5C+that%5C+the%5C+choromosome%5C+number%5C+of%5C+all%5C+the%5C+populations%5C+is%5C+2n%5C+%3D%5C+18.%5C+The%5C+interphase%5C+nuclei%5C+and%5C+prophase%5C+chromosomes%5C+of%5C+the%5C+species%5C+were%5C+found%5C+to%5C+be%5C+of%5C+the%5C+complex%5C+chromosome%5C+type%5C+and%5C+interstitial%5C+type.%5C+The%5C+results%5C+of%5C+karyotype%5C+analysis%5C+showed%5C+that%5C+7%5C+of%5C+14%5C+materials%5C+has%5C+satellites%2C%5C+and%5C+the%5C+number%5C+and%5C+position%5C+of%5C+satellites%5C+differ%5C+among%5C+populations%2C%5C+and%5C+thus%5C+revealed%5C+a%5C+series%5C+of%5C+diversified%5C+karyotypes.%5C+With%5C+most%5C+populations%5C+being%5C+of%5C+ploidy%2C%5C+cytogenetical%5C+divergence%5C+within%5C+the%5C+species%5C+lied%5C+mainly%5C+in%5C+chromosome%5C+size%5C+and%5C+structure.%5C+The%5C+fact%5C+that%5C+polyploidization%5C+did%5C+not%5C+occur%5C+very%5C+often%5C+for%5C+variations%5C+in%5C+Southwest%5C+China%5C+was%5C+against%5C+viewpoint%5C+that%5C+polyploidization%5C+level%5C+in%5C+this%5C+area%5C+is%5C+higher%5C+than%5C+that%5C+of%5C+other%5C+distribution%5C+areas%5C+due%5C+to%5C+the%5C+elevation%5C+of%5C+mountains%5C+and%5C+plateau.%5C+2.%5C+Phylogeographic%5C+analysisbased%5C+on%5C+chloroplast%5C+sequence%EF%BC%8CWe%5C+sequenced%5C+two%5C+cpDNA%5C+fragments%5C+rpl32%5C-trnL%5C%28UAG%5C%29intergenic%5C+spacer%5C+and%5C+trnH%5C-psbA%5C+spacer%5C+in%5C+40%5C+populations%5C+sampled%2C%5C+recovering%5C+22%5C+chlorotypes.%5C+The%5C+average%5C+with%5C-in%5C+population%5C+diversity%5C+%5C%28hS%5C+%3D%5C+0.171%5C%29%5C+was%5C+much%5C+lower%5C+than%5C+total%5C+genetic%5C+diversity%5C+%5C%28hT%5C+%3D%5C+0.857%5C%29.%5C+Population%5C+differentiation%5C+was%5C+high%5C+%5C%28NST%5C+%3D%5C+0.924%2C%5C+GST%5C+%3D%5C+0.801%5C%29%5C+indicating%5C+low%5C+levels%5C+of%5C+seed%5C-based%5C+gene%5C+flow%5C+and%5C+significant%5C+phylogeographical%5C+stucture%5C+%5C%28NST%5C+%3E%5C+GST%2C%5C+P%5C+%3C%5C+0.05%5C%29%5C+were%5C+exhibited%5C+by%5C+this%5C+species.%5C+The%5C+SAMOVA%5C+revealed%5C+seven%5C+diverging%5C+groups%5C+of%5C+related%5C+chlorotypes%2C%5C+six%5C+of%5C+them%5C+had%5C+distinct%5C+nonoverlapping%5C+geographical%5C+ranges%5C%3A%5C+one%5C+in%5C+the%5C+northeast%5C+comprising%5C+10%5C+populations%2C%5C+a%5C+second%5C+with%5C+a%5C+southeast%5C+distribution%5C+comprising%5C+22%5C+populations%2C%5C+and%5C+the%5C+remaning%5C+four%5C+groups%5C+comprising%5C+15%5C+populations%5C+located%5C+in%5C+the%5C+west%5C+part%5C+of%5C+the%5C+species%E2%80%99%5C+range%5C+along%5C+different%5C+river%5C+valleys.%5C+The%5C+genetic%5C+clustering%5C+of%5C+populations%5C+into%5C+three%5C+regions%5C+was%5C+also%5C+supported%5C+by%5C+analysis%5C+of%5C+molecular%5C+variance%2C%5C+which%5C+showed%5C+that%5C+most%5C+genetic%5C+variation%5C+%5C%2882.43%25%5C%29%5C+was%5C+found%5C+among%5C+these%5C+three%5C+regions.%5C+Two%5C+clusters%5C+were%5C+distinguished%5C+by%5C+both%5C+phylogenetic%5C+analysis%5C+and%5C+genealogical%5C+analysis%5C+of%5C+chlorotypes%2C%5C+one%5C+consisting%5C+of%5C+chlorotypes%5C+from%5C+the%5C+western%5C+region%5C+and%5C+the%5C+second%5C+consisting%5C+of%5C+those%5C+from%5C+the%5C+eastern%5C+region.%5C+Significant%5C+genetic%5C+differences%5C+between%5C+the%5C+two%5C+regions%5C+might%5C+be%5C+attributed%5C+to%5C+vicariance%5C+and%5C+restricted%5C+gene%5C+flow%2C%5C+and%5C+this%5C+vicariance%5C+could%5C+be%5C+explained%5C+by%5C+the%5C+physical%5C+environmental%5C+heterogeneity%5C+on%5C+each%5C+side%5C+of%5C+the%5C+Tanaka%5C-Kaiyong%5C+Line.%5C+Following%5C+the%5C+uplift%5C+of%5C+the%5C+Tibetan%5C+Plateau%2C%5C+the%5C+reorganization%5C+of%5C+the%5C+major%5C+river%5C+drainages%5C+was%5C+primarily%5C+caused%5C+by%5C+river%5C+separation%5C+and%5C+capture%5C+events.%5C+These%5C+historical%5C+events%5C+could%5C+change%5C+the%5C+distribution%5C+of%5C+S.%5C+davidii%5C+from%5C+fragmented%5C+to%5C+continuous%5C+%5C%28Upper%5C%2FLower%5C+Jinshajiang%5C+and%5C+Yalongjiang%5C%2FDaduhe%5C%29%2C%5C+and%5C+from%5C+continuous%5C+to%5C+fragmented%5C+%5C%28Nujiang%5C+and%5C+Jinshajiang%5C%2FHonghe%5C%29.%5C+However%2C%5C+spatial%5C+and%5C+temporal%5C+patterns%5C+of%5C+phylogeographic%5C+divergence%5C+are%5C+strongly%5C+associated%5C+with%5C+historical%5C+disjunction%5C+rather%5C+than%5C+modern%5C+drainage%5C+connections.%5C+Moreover%2C%5C+the%5C+following%5C+north%5C-south%5C+split%5C+in%5C+the%5C+eastern%5C+region%5C+and%5C+effective%5C+isolation%5C+with%5C+their%5C+genetic%5C+diversity%5C+were%5C+essentially%5C+modelled%5C+by%5C+genetic%5C+drift.%5C+The%5C+higher%5C+chlorotype%5C+richness%5C+and%5C+genetic%5C+divergence%5C+for%5C+populations%5C+in%5C+western%5C+region%5C+compared%5C+with%5C+other%5C+two%5C+regions%5C+suggests%5C+that%5C+there%5C+were%5C+multipe%5C+refugia%5C+or%5C+in%5C+situ%5C+survival%5C+of%5C+S.%5C+davidii%5C+in%5C+the%5C+Himalayan%5C-Hengduan%5C+Mountain%5C+region.%5C+Fixation%5C+of%5C+chlorotypes%5C+in%5C+the%5C+northeastern%5C+region%5C+and%5C+near%5C+fixation%5C+in%5C+the%5C+southeastern%5C+region%5C+suggest%5C+a%5C+recent%5C+colonization%5C+of%5C+these%5C+areas.%5C+We%5C+further%5C+found%5C+that%5C+this%5C+species%5C+underwent%5C+past%5C+range%5C+expansion%5C+around%5C+37%5C-303%5C+thousand%5C+years%5C+ago%5C+%5C%28kya%5C%29.%5C+The%5C+southeastern%5C+populations%5C+likely%5C+experienced%5C+a%5C+demographic%5C+expansion%5C+via%5C+unidirectional%5C+gene%5C+flow%5C+along%5C+rivers%2C%5C+while%5C+northeastern%5C+populations%5C+underwent%5C+a%5C+more%5C+northward%5C+expansion%2C%5C+both%5C+from%5C+initial%5C+populations%5C+%5C%28s%5C%29%5C+%5C%2821%2C%5C+22%2C%5C+23%5C%29%5C+preserved%5C+on%5C+eastern%5C+refugia%5C+%5C%28Jinshajiang%5C%29.%5C+This%5C+process%5C+might%5C+have%5C+been%5C+accompanied%5C+with%5C+a%5C+series%5C+of%5C+founder%5C+effects%5C+or%5C+bottlenecks%5C+making%5C+populations%5C+genetically%5C+impoverished.%5C+3.%5C+Phylogeographic%5C+analysisbased%5C+on%5C+nuclear%5C+sequence%EF%BC%8CWe%5C+sequenced%5C+the%5C+nuclear%5C+%5C%28ncpGS%5C%29%5C+region%5C+in%5C+all%5C+populations%5C+sampled%2C%5C+recovering%5C+23%5C+nuclear%5C+haplotypes.%5C+Compared%5C+to%5C+cpDNA%2C%5C+both%5C+NST%5C+%5C%280.470%5C%29%5C+and%5C+GST%5C+%5C%280.338%5C%29%5C+were%5C+relatively%5C+lower%2C%5C+but%5C+NST%5C+was%5C+also%5C+significantly%5C+larger%5C+than%5C+GST.%5C+37.10%25%5C+of%5C+the%5C+total%5C+variation%5C+was%5C+distributed%5C+among%5C+regions%5C+which%5C+was%5C+much%5C+lower%5C+than%5C+that%5C+shown%5C+by%5C+chlorotypes.%5C+Thus%2C%5C+more%5C+extensive%5C+distribution%5C+of%5C+nuclear%5C+haplotypes%5C+was%5C+exhibited%5C+across%5C+the%5C+geographical%5C+range%5C+instead%5C+of%5C+the%5C+strong%5C+population%5C+subdivision%5C+observed%5C+in%5C+chlorotypes.%5C+Similarly%5C+to%5C+the%5C+chloroplast%5C+data%2C%5C+we%5C+found%5C+that%5C+genetic%5C+differentiation%5C+of%5C+nDNA%5C+was%5C+positively%5C+correlated%5C+with%5C+the%5C+geographical%5C+distance%2C%5C+but%5C+the%5C+increase%5C+in%5C+the%5C+geographical%5C+distance%5C+between%5C+populations%5C+did%5C+not%5C+increase%5C+the%5C+genetic%5C+differentiation%5C+of%5C+nDNA%5C+as%5C+rapidly%5C+as%5C+that%5C+of%5C+cpDNA.%5C+These%5C+contrasting%5C+levels%5C+between%5C+the%5C+chloroplast%5C+and%5C+nuclear%5C+genomes%5C+of%5C+S.%5C+davidii%5C+are%5C+likely%5C+due%5C+to%5C+limited%5C+gene%5C+flow%5C+of%5C+cpDNA%5C+by%5C+seeds%5C+vs.%5C+the%5C+extensive%5C+gene%5C+flow%5C+of%5C+nDNA%5C+by%5C+wind%5C-mediated%5C+pollen%5C+in%5C+the%5C+population%5C+history.%5C+We%5C+also%5C+determined%5C+from%5C+nuclear%5C+markers%5C+that%5C+haplotype%5C+diversity%5C+was%5C+reduced%5C+in%5C+the%5C+southeastern%5C+and%5C+northeastern%5C+regions%5C+due%5C+to%5C+the%5C+loss%5C+of%5C+rare%5C+haplotypes%5C+in%5C+western%5C+region.%5C+This%5C+reduction%5C+of%5C+gene%5C+diversity%5C+is%5C+also%5C+a%5C+signature%5C+of%5C+founder%5C+events%5C+or%5C+recent%5C+bottleneck%5C+during%5C+post%5C-glacial%5C+colonization.%5C+However%2C%5C+nuclear%5C+diversity%5C+within%5C+populations%5C+remains%5C+high.%5C+This%5C+provides%5C+evidence%5C+that%5C+regionally%5C+pollen%5C+flow%5C+might%5C+be%5C+sufficiently%5C+high%5C+to%5C+blur%5C+the%5C+genetic%5C+identity%5C+of%5C+founder%5C+populations%5C+over%5C+a%5C+reasonably%5C+large%5C+spatial%5C+scale.3.%5C+Relationships%5C+among%5C+three%5C+varieties%EF%BC%8CThe%5C+phylogenetic%5C+analysis%5C+identified%5C+two%5C+phylogroups%5C+of%5C+chlorotypes%2C%5C+corresponding%5C+to%5C+S.%5C+davidii%5C+var.%5C+davidii%5C+and%5C+var.%5C+chuansinesis.%5C+The%5C+former%5C+was%5C+distinguished%5C+by%5C+the%5C+abscence%5C+of%5C+predonminant%5C+nuclear%5C+haplotype%5C+H1%5C+of%5C+the%5C+latter.%5C+The%5C+monophyletic%5C+group%5C+of%5C+chlorotypes%5C+in%5C+var.%5C+davidii%5C+and%5C+var.%5C+liangshanesis%5C+showed%5C+their%5C+relatively%5C+close%5C+relationship.%5C+And%5C+their%5C+genetic%5C+divergence%5C+from%5C+the%5C+third%5C+variety%5C+appears%5C+to%5C+be%5C+relative%5C+to%5C+their%5C+slight%5C+morphological%5C+difference%5C+in%5C+leaf%5C+size%5C+and%5C+the%5C+divergent%5C+environmental%5C+niche%5C+spaces%5C+they%5C+occupy.%5C+Thus%2C%5C+the%5C+observed%5C+differences%5C+in%5C+morphological%5C+characters%5C+between%5C+var.%5C+chuansinesis%5C+and%5C+other%5C+two%5C+varieties%5C+can%5C+be%5C+explained%5C+by%5C+the%5C+seed%5C+dispersal%5C+limitation%5C+illustrated%5C+above%5C+%5C%28as%5C+inferred%5C+by%5C+geographical%5C+separation%5C%29%5C+and%5C+by%5C+environmental%5C+heterogeneity%5C+%5C%28as%5C+inferred%5C+by%5C+precipitation%5C+or%5C+elevation%5C%29%5C+or%5C+by%5C+a%5C+combination%5C+of%5C+both.%5C+After%5C+all%2C%5C+the%5C+geological%5C+changes%2C%5C+drainage%5C+reorganization%2C%5C+and%5C+floristic%5C+differences%5C+following%5C+the%5C+Himalayan%5C+uplift%5C+have%5C+been%5C+suggested%5C+to%5C+affect%5C+the%5C+genetic%5C+structure%5C+of%5C+S.%5C+davidii.%5C+These%5C+results%5C+provide%5C+new%5C+insights%5C+into%5C+the%5C+phylogeographic%5C+pattern%5C+of%5C+plants%5C+in%5C+China.%5C+In%5C+addition%2C%5C+the%5C+unique%5C+population%5C+genetic%5C+structure%5C+found%5C+in%5C+S.%5C+davidii%5C+has%5C+provided%5C+important%5C+insights%5C+into%5C+the%5C+evolutionary%5C+history%5C+of%5C+this%5C+species.%5C+The%5C+genetic%5C+profile%5C+uncovered%5C+in%5C+this%5C+study%5C+is%5C+also%5C+critical%5C+for%5C+its%5C+conservation%5C+management.%5C+Our%5C+study%5C+has%5C+uncovered%5C+the%5C+existence%5C+of%5C+at%5C+least%5C+two%5C+%E2%80%98evolutionary%5C+significant%5C+units%E2%80%99%5C+independent%5C+units%5C+within%5C+S.%5C+davidii%2C%5C+corresponding%5C+to%5C+var.%5C+davidii%5C+from%5C+eastern%5C+region%5C+and%5C+var.%5C+chuansinensis%5C+from%5C+western%5C+region.%5C+The%5C+conservation%5C+efforts%5C+should%5C+first%5C+focus%5C+on%5C+most%5C+western%5C+populations%5C+and%5C+on%5C+the%5C+southeastern%5C+ones%5C+exhibiting%5C+high%5C+levels%5C+of%5C+genetic%5C+diversity%2C%5C+while%5C+the%5C+genetically%5C+homogeneous%5C+northeastern%5C+populations%5C+located%5C+in%5C+the%5C+degraded%5C+Loess%5C+Plateau%5C+should%5C+require%5C+much%5C+greater%5C+conservation%5C+efforts."},{"jsname":"The Taxus wallichiana complex represents an old relict conifer lineage that survived through the Tertiary. It is currently distributed in the mountain forests in South and Southwest China south of the Qinling Mountains. In the present study, we explored phylogeography of the complex by using two chloroplast DNA regions, one nuclear ribosomal DNA spacer region and eight microsatellite (SSR) loci. The main conclusions can be summarized as follows:1. Phylogeographic pattern based on chloroplast haplotypes，There were 11 cpDNA haplotypes identified in the T. wallichiana complex The complex showed a high level of genetic diversity and obvious genetic differentiation. The 44 sampled populations showed obvious genetic structure, which could be divided into five groups, namely the Huanan group, the Daba group, the Emei group, the Yunnan group and the Qinling group. There was extremely high genetic differentiation among groups, but not significant within group. The divergence times of the five lineages, estimated using average mutation rates of trnL-trnF, fell in the Pliocene. 2. Phylogeographic patterns based on ITS sequences，These included 38 unique ‘haplotypes’ based on ITS data. Their analysis showed that the T. wallichiana complex possessed a high genetic diversity. These populations could be divided into four groups, namely the Huanan group, the Daba/Emei group, the Yunnan group and the Qinling group. Based on all results, it appears that the major lineages constituting the T. wallichiana complex have arisen before Quaternary glaciation cycles, and may have survived isolated in different refugia. During interglacial periods some lineages appear to have come in contact and hybridizedbut other lineages merged forming populations with mixed haplotypes without signs of hybridization. The present-day phylogeographical distribution pattern of the T. wallichiana complex might thus be the result of repeated expansion / contractions of populations during interglacial / glacial cycles.3. Population genetic analysis using microsatellite (SSR) markers，Eight SSR loci were used for population genetic analysis on the T. wallichiana complex. A lower level of genetic diversity at the population level and high genetic differentiation among population was detected. The results of structure analysis were similar to those on the ITS data, dividing the populations into four groups (lineages). According to the results here, it was deduced that each of the 4 lineages of the T. wallichiana complex may possessed respective glacial refugia, and some lineages (such as the Qinling and Huanan lineage) might have survived in multiple refugia in the Quaternay glaciations. The present distribution pattern of this complex was likely influenced by the uplift of the QTP and Quaternary glaciation.","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&fq=dc.date.issued.year%3A2010&sort_by=2&isNonaffiliated=false&search_type=-1&query1=Clones&order=desc&&fq=dc.project.title_filter%3AThe%5C+Taxus%5C+wallichiana%5C+complex%5C+represents%5C+an%5C+old%5C+relict%5C+conifer%5C+lineage%5C+that%5C+survived%5C+through%5C+the%5C+Tertiary.%5C+It%5C+is%5C+currently%5C+distributed%5C+in%5C+the%5C+mountain%5C+forests%5C+in%5C+South%5C+and%5C+Southwest%5C+China%5C+south%5C+of%5C+the%5C+Qinling%5C+Mountains.%C2%A0In%5C+the%5C+present%5C+study%2C%5C+we%5C+explored%5C+phylogeography%5C+of%5C+the%5C+complex%5C+by%5C+using%5C+two%5C+chloroplast%5C+DNA%5C+regions%2C%5C+one%5C+nuclear%5C+ribosomal%5C+DNA%5C+spacer%5C+region%5C+and%5C+eight%5C+microsatellite%5C+%5C%28SSR%5C%29%5C+loci.%5C+The%5C+main%5C+conclusions%5C+can%5C+be%5C+summarized%5C+as%5C+follows%5C%3A1.%5C+Phylogeographic%5C+pattern%5C+based%5C+on%5C+chloroplast%5C+haplotypes%EF%BC%8CThere%5C+were%5C+11%5C+cpDNA%5C+haplotypes%5C+identified%5C+in%5C+the%5C+T.%5C+wallichiana%5C+complex%5C+The%5C+complex%5C+showed%5C+a%5C+high%5C+level%5C+of%5C+genetic%5C+diversity%5C+and%5C+obvious%5C+genetic%5C+differentiation.%5C+The%5C+44%5C+sampled%5C+populations%5C+showed%5C+obvious%5C+genetic%5C+structure%2C%5C+which%5C+could%5C+be%5C+divided%5C+into%5C+five%5C+groups%2C%5C+namely%5C+the%5C+Huanan%5C+group%2C%5C+the%5C+Daba%5C+group%2C%5C+the%5C+Emei%5C+group%2C%5C+the%5C+Yunnan%5C+group%5C+and%5C+the%5C+Qinling%5C+group.%5C+There%5C+was%5C+extremely%5C+high%5C+genetic%5C+differentiation%5C+among%5C+groups%2C%5C+but%5C+not%5C+significant%5C+within%5C+group.%5C+The%5C+divergence%5C+times%5C+of%5C+the%5C+five%5C+lineages%2C%5C+estimated%5C+using%5C+average%5C+mutation%5C+rates%5C+of%5C+trnL%5C-trnF%2C%5C+fell%5C+in%5C+the%5C+Pliocene.%C2%A02.%5C+Phylogeographic%5C+patterns%5C+based%5C+on%5C+ITS%5C+sequences%EF%BC%8CThese%5C+included%5C+38%5C+unique%5C+%E2%80%98haplotypes%E2%80%99%5C+based%5C+on%5C+ITS%5C+data.%5C+Their%5C+analysis%5C+showed%5C+that%5C+the%5C+T.%5C+wallichiana%5C+complex%5C+possessed%5C+a%5C+high%5C+genetic%5C+diversity.%C2%A0These%5C+populations%5C+could%5C+be%5C+divided%5C+into%5C+four%5C+groups%2C%5C+namely%5C+the%5C+Huanan%5C+group%2C%5C+the%5C+Daba%5C%2FEmei%5C+group%2C%5C+the%5C+Yunnan%5C+group%5C+and%5C+the%5C+Qinling%5C+group.%5C+Based%5C+on%5C+all%5C+results%2C%5C+it%5C+appears%5C+that%5C+the%5C+major%5C+lineages%5C+constituting%5C+the%5C+T.%5C+wallichiana%5C+complex%5C+have%5C+arisen%5C+before%5C+Quaternary%5C+glaciation%5C+cycles%2C%5C+and%5C+may%5C+have%5C+survived%5C+isolated%5C+in%5C+different%5C+refugia.%5C+During%5C+interglacial%5C+periods%5C+some%5C+lineages%5C+appear%5C+to%5C+have%5C+come%5C+in%5C+contact%5C+and%5C+hybridizedbut%5C+other%5C+lineages%5C+merged%5C+forming%5C+populations%5C+with%5C+mixed%5C+haplotypes%5C+without%5C+signs%5C+of%5C+hybridization.%5C+The%5C+present%5C-day%5C+phylogeographical%5C+distribution%5C+pattern%5C+of%5C+the%5C+T.%5C+wallichiana%5C+complex%5C+might%5C+thus%5C+be%5C+the%5C+result%5C+of%5C+repeated%5C+expansion%5C+%5C%2F%5C+contractions%5C+of%5C+populations%5C+during%5C+interglacial%5C+%5C%2F%5C+glacial%5C+cycles.3.%5C+Population%5C+genetic%5C+analysis%5C+using%5C+microsatellite%5C+%5C%28SSR%5C%29%5C+markers%EF%BC%8CEight%5C+SSR%5C+loci%5C+were%5C+used%5C+for%5C+population%5C+genetic%5C+analysis%5C+on%5C+the%5C+T.%5C+wallichiana%5C+complex.%5C+A%5C+lower%5C+level%5C+of%5C+genetic%5C+diversity%5C+at%5C+the%5C+population%5C+level%5C+and%5C+high%5C+genetic%5C+differentiation%5C+among%5C+population%5C+was%5C+detected.%5C+The%5C+results%5C+of%5C+structure%5C+analysis%5C+were%5C+similar%5C+to%5C+those%5C+on%5C+the%5C+ITS%5C+data%2C%5C+dividing%5C+the%5C+populations%5C+into%5C+four%5C+groups%5C+%5C%28lineages%5C%29.%C2%A0According%5C+to%5C+the%5C+results%5C+here%2C%5C+it%5C+was%5C+deduced%5C+that%5C+each%5C+of%5C+the%5C+4%5C+lineages%5C+of%5C+the%5C+T.%5C+wallichiana%5C+complex%5C+may%5C+possessed%5C+respective%5C+glacial%5C+refugia%2C%5C+and%5C+some%5C+lineages%5C+%5C%28such%5C+as%5C+the%5C+Qinling%5C+and%5C+Huanan%5C+lineage%5C%29%5C+might%5C+have%5C+survived%5C+in%5C+multiple%5C+refugia%5C+in%5C+the%5C+Quaternay%5C+glaciations.%5C+The%5C+present%5C+distribution%5C+pattern%5C+of%5C+this%5C+complex%5C+was%5C+likely%5C+influenced%5C+by%5C+the%5C+uplift%5C+of%5C+the%5C+QTP%5C+and%5C+Quaternary%5C+glaciation."},{"jsname":"The temperate woody bamboos are a morphologically diverse group with a complicated taxonomy. The Arundinaria group has an East Asia-North America disjunct distribution, which is one of those with complex taxonomy in the temperate woody bamboos. In this study, the phylogeny of the temperate woody bamboos was reconstructed based on eight non-coding regions of the chloroplast genome and nuclear gene GBSSI using large sample set (124 species in 24 genera) with an emphasis on the Arundinaria group. The monophyly of the temperate woody bamboos was resolved in all phylogenies. Ten major lineages were obtained in the chloroplast phylogeny with unresolved relationships among them; the recovered phylogeny is strongly incongruent with the classifications based on morphology at both subtribal and generic ranks; some subclades that are related to the geographic distribution were obtained in those lineages. Five lineages in the GBSSI gene phylogeny were recovered as the same in the chloroplast phylogeny, and the other lineages were incongruent with chloroplast phylogeny in some ways. The reticulate evolution caused by hybridization, introgression and lineage sorting may be an explanation for the molecular phylogenetic incongruence. Based on the facts of diverse morphology, broad distribution and molecular phylogeny, we inferred that the major clades and species within most of the clades of the temperate woody bamboos were originated during several rapid adaptive radiations. Ten putative hybrids were discussed based on molecular phylogenies, morphology and distribution. The micromorphology of the leaf epidermis under SEM (scanning electron microscope) was observed and divided into nine types; the micromorphology can provide some evidence for the bamboo taxonomy and inference of putative hybrids. Additionally, taxonomic revisions were presented for some species based on field observation and herbarium work.","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&fq=dc.date.issued.year%3A2010&sort_by=2&isNonaffiliated=false&search_type=-1&query1=Clones&order=desc&&fq=dc.project.title_filter%3AThe%5C+temperate%5C+woody%5C+bamboos%5C+are%5C+a%5C+morphologically%5C+diverse%5C+group%5C+with%5C+a%5C+complicated%5C+taxonomy.%5C+The%5C+Arundinaria%5C+group%5C+has%5C+an%5C+East%5C+Asia%5C-North%5C+America%5C+disjunct%5C+distribution%2C%5C+which%5C+is%5C+one%5C+of%5C+those%5C+with%5C+complex%5C+taxonomy%5C+in%5C+the%5C+temperate%5C+woody%5C+bamboos.%5C+In%5C+this%5C+study%2C%5C+the%5C+phylogeny%5C+of%5C+the%5C+temperate%5C+woody%5C+bamboos%5C+was%5C+reconstructed%5C+based%5C+on%5C+eight%5C+non%5C-coding%5C+regions%5C+of%5C+the%5C+chloroplast%5C+genome%5C+and%5C+nuclear%5C+gene%5C+GBSSI%5C+using%5C+large%5C+sample%5C+set%5C+%5C%28124%5C+species%5C+in%5C+24%5C+genera%5C%29%5C+with%5C+an%5C+emphasis%5C+on%5C+the%5C+Arundinaria%5C+group.%5C+The%5C+monophyly%5C+of%5C+the%5C+temperate%5C+woody%5C+bamboos%5C+was%5C+resolved%5C+in%5C+all%5C+phylogenies.%5C+Ten%5C+major%5C+lineages%5C+were%5C+obtained%5C+in%5C+the%5C+chloroplast%5C+phylogeny%5C+with%5C+unresolved%5C+relationships%5C+among%5C+them%5C%3B%5C+the%5C+recovered%5C+phylogeny%5C+is%5C+strongly%5C+incongruent%5C+with%5C+the%5C+classifications%5C+based%5C+on%5C+morphology%5C+at%5C+both%5C+subtribal%5C+and%5C+generic%5C+ranks%5C%3B%5C+some%5C+subclades%5C+that%5C+are%5C+related%5C+to%5C+the%5C+geographic%5C+distribution%5C+were%5C+obtained%5C+in%5C+those%5C+lineages.%5C+Five%5C+lineages%5C+in%5C+the%5C+GBSSI%5C+gene%5C+phylogeny%5C+were%5C+recovered%5C+as%5C+the%5C+same%5C+in%5C+the%5C+chloroplast%5C+phylogeny%2C%5C+and%5C+the%5C+other%5C+lineages%5C+were%5C+incongruent%5C+with%5C+chloroplast%5C+phylogeny%5C+in%5C+some%5C+ways.%5C+The%5C+reticulate%5C+evolution%5C+caused%5C+by%5C+hybridization%2C%5C+introgression%5C+and%5C+lineage%5C+sorting%5C+may%5C+be%5C+an%5C+explanation%5C+for%5C+the%5C+molecular%5C+phylogenetic%5C+incongruence.%5C+Based%5C+on%5C+the%5C+facts%5C+of%5C+diverse%5C+morphology%2C%5C+broad%5C+distribution%5C+and%5C+molecular%5C+phylogeny%2C%5C+we%5C+inferred%5C+that%5C+the%5C+major%5C+clades%5C+and%5C+species%5C+within%5C+most%5C+of%5C+the%5C+clades%5C+of%5C+the%5C+temperate%5C+woody%5C+bamboos%5C+were%5C+originated%5C+during%5C+several%5C+rapid%5C+adaptive%5C+radiations.%5C+Ten%5C+putative%5C+hybrids%5C+were%5C+discussed%5C+based%5C+on%5C+molecular%5C+phylogenies%2C%5C+morphology%5C+and%5C+distribution.%5C+The%5C+micromorphology%5C+of%5C+the%5C+leaf%5C+epidermis%5C+under%5C+SEM%5C+%5C%28scanning%5C+electron%5C+microscope%5C%29%5C+was%5C+observed%5C+and%5C+divided%5C+into%5C+nine%5C+types%5C%3B%5C+the%5C+micromorphology%5C+can%5C+provide%5C+some%5C+evidence%5C+for%5C+the%5C+bamboo%5C+taxonomy%5C+and%5C+inference%5C+of%5C+putative%5C+hybrids.%5C+Additionally%2C%5C+taxonomic%5C+revisions%5C+were%5C+presented%5C+for%5C+some%5C+species%5C+based%5C+on%5C+field%5C+observation%5C+and%5C+herbarium%5C+work."},{"jsname":"Transposable elements (TEs) have been found to be a significant fraction of eukaryotic genomes. Moreover, they make great contributions to the structure, function and evolution of genomes as well as genes. However, some questions such as the mechanisms of retainment of TEs in the genome and their adaptive evolution have not been fully elucidated so far. In this study, the distributions of 17 TE-gene associations among Oryza species were investigated. In addition, the nucleotide diversity was analysed and neutral tests for the region flanking the TE insertions were performed. Based on the above-observed patterns, evolutionary relationships between species in the AA genome group were discussed. The main results are as follows: For each TE-gene association, PCR and electrophoresis were conducted for a total of 107 strains, belonging to different Oryza species. The patterns of each TE-gene association in different species were obtained. It is our finding that 2 associations distribute through all Oryza species. By contrast, other 15 associations were only observed in some Oryza species. On basis of the above-mentioned results, it is likely that insertion events under study occurred in their common ancestor, and then they dispersed with subsequent divergence of different AA genome species. Our datas strongly support that O. meridionalis is the most basal lineage of AA genome group, instead of O. longistaminata.For several TE-gene associations fixed in populations of ancestor, the nucleotide diversity was estimated and neutral tests for the region flanking the TE insertions between populations with and without TE insertions were performed. No significant result was obtained. It is possible that the fixation of mutations with TE insetion is a random process; alternatively, this process is attributable to nature selection. Since the fixation has finished, it is difficult to detect the signature at the sequence level.","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&fq=dc.date.issued.year%3A2010&sort_by=2&isNonaffiliated=false&search_type=-1&query1=Clones&order=desc&&fq=dc.project.title_filter%3ATransposable%5C+elements%5C+%5C%28TEs%5C%29%5C+have%5C+been%5C+found%5C+to%5C+be%5C+a%5C+significant%5C+fraction%5C+of%5C+eukaryotic%5C+genomes.%5C+Moreover%2C%5C+they%5C+make%5C+great%5C+contributions%5C+to%5C+the%5C+structure%2C%5C+function%5C+and%5C+evolution%5C+of%5C+genomes%5C+as%5C+well%5C+as%5C+genes.%5C+However%2C%5C+some%5C+questions%5C+such%5C+as%5C+the%5C+mechanisms%5C+of%5C+retainment%5C+of%5C+TEs%5C+in%5C+the%5C+genome%5C+and%5C+their%5C+adaptive%5C+evolution%5C+have%5C+not%5C+been%5C+fully%5C+elucidated%5C+so%5C+far.%5C+In%5C+this%5C+study%2C%5C+the%5C+distributions%5C+of%5C+17%5C+TE%5C-gene%5C+associations%5C+among%5C+Oryza%5C+species%5C+were%5C+investigated.%5C+In%5C+addition%2C%5C+the%5C+nucleotide%5C+diversity%5C+was%5C+analysed%5C+and%5C+neutral%5C+tests%5C+for%5C+the%5C+region%5C+flanking%5C+the%5C+TE%5C+insertions%5C+were%5C+performed.%5C+Based%5C+on%5C+the%5C+above%5C-observed%5C+patterns%2C%5C+evolutionary%5C+relationships%5C+between%5C+species%5C+in%5C+the%5C+AA%5C+genome%5C+group%5C+were%5C+discussed.%5C+The%5C+main%5C+results%5C+are%5C+as%5C+follows%5C%3A%5C+For%5C+each%5C+TE%5C-gene%5C+association%2C%5C+PCR%5C+and%5C+electrophoresis%5C+were%5C+conducted%5C+for%5C+a%5C+total%5C+of%5C+107%5C+strains%2C%5C+belonging%5C+to%5C+different%5C+Oryza%5C+species.%5C+The%5C+patterns%5C+of%5C+each%5C+TE%5C-gene%5C+association%5C+in%5C+different%5C+species%5C+were%5C+obtained.%5C+It%5C+is%5C+our%5C+finding%5C+that%5C+2%5C+associations%5C+distribute%5C+through%5C+all%5C+Oryza%5C+species.%5C+By%5C+contrast%2C%5C+other%5C+15%5C+associations%5C+were%5C+only%5C+observed%5C+in%5C+some%5C+Oryza%5C+species.%5C+On%5C+basis%5C+of%5C+the%5C+above%5C-mentioned%5C+results%2C%5C+it%5C+is%5C+likely%5C+that%5C+insertion%5C+events%5C+under%5C+study%5C+occurred%5C+in%5C+their%5C+common%5C+ancestor%2C%5C+and%5C+then%5C+they%5C+dispersed%5C+with%5C+subsequent%5C+divergence%5C+of%5C+different%5C+AA%5C+genome%5C+species.%5C+Our%5C+datas%5C+strongly%5C+support%5C+that%5C+O.%5C+meridionalis%5C+is%5C+the%5C+most%5C+basal%5C+lineage%5C+of%5C+AA%5C+genome%5C+group%2C%5C+instead%5C+of%5C+O.%5C+longistaminata.For%5C+several%5C+TE%5C-gene%5C+associations%5C+fixed%5C+in%5C+populations%5C+of%5C+ancestor%2C%5C+the%5C+nucleotide%5C+diversity%5C+was%5C+estimated%5C+and%5C+neutral%5C+tests%5C+for%5C+the%5C+region%5C+flanking%5C+the%5C+TE%5C+insertions%5C+between%5C+populations%5C+with%5C+and%5C+without%5C+TE%5C+insertions%5C+were%5C+performed.%5C+No%5C+significant%5C+result%5C+was%5C+obtained.%5C+It%5C+is%5C+possible%5C+that%5C+the%5C+fixation%5C+of%5C+mutations%5C+with%5C+TE%5C+insetion%5C+is%5C+a%5C+random%5C+process%5C%3B%5C+alternatively%2C%5C+this%5C+process%5C+is%5C+attributable%5C+to%5C+nature%5C+selection.%5C+Since%5C+the%5C+fixation%5C+has%5C+finished%2C%5C+it%5C+is%5C+difficult%5C+to%5C+detect%5C+the%5C+signature%5C+at%5C+the%5C+sequence%5C+level."},{"jsname":"Trigonobalanus doichangensis is an endangered plant. In this paper, the megasporogenesis and development of female gametophyte, seed morphological traits and seed germination, seed conservation, micropropagation and acclimatization of this species were studied. Combined with the published results of cytology, molecular genetics and other researches,the mechanisms of extinction, basic biology and technology of germplasm conservation and acclimatization of T. doichangensis were discussed. The main results are summarized as follows:1. Megasporogenesis and development of female gametophyte，Stamens exist under the stigma of T. doichangensis, and the pollen is aborted on the later development stage of pistil, therefore, the pistillate flower in function is hermaphrodite flower in morphology. The ovule is anatropous, bitegmic and crassinucellate. The primary archesporium is hypodermal and single-celled and the sporogenous cell of the nucellus functions directly as a megaspore mother cell which goes meiosis to form a linear tetrad. The chalazal megaspore of the tetrad is functional. The development of embryo sac conforms to the polygonum type. There are six ovules in the ovary of T. doichangensis, and only one develops into a seed in normal fruits. In the process of megasporogenesis and development of female gametophyte, there are several links of abortion, and 93.3% of mature embryo sacs is aborted.2. Morphological characters and germination of seeds，Most of the variation occurred among individual trees within populations in seed morphological traits (length, width and 1000-seed weight) and germination-related indices (germination percentage, germination index and vigor index). In addition, the variation in percentage of well-developed seeds among populations and among individual trees within populations is equal, each accounting for 48%. Each of seed morphological traits has significantly positive correlation with each other (p < 0.01), but they have no significant correlation with percentage of well-developed seeds and germination-related indices. In the same batch of seeds of T. doichangensis, there are light-colored and dark-colored seed coats, and development of light-colored seeds is significantly poorer than that of dark-colored seeds.The sensitivity of seeds to high temperature varys in different stages of seed imbibition. In each stage, heat acclimatization don’t increase germination percentage, germination index and fresh weight of seedlings. If the distilled water is substituted by solution of SA during seed imbibition, seed germination and germination index after heat shock are not significantly different from control, but they are significantly higher than that of other treatments. Moreover, when the seeds are treatmented with SA, the fresh weight of seedlings is significantly higher than that of control and other treatments.3. Seed conservation，Seeds of T. doichangensis belong to orthodox seeds which can tolerate certain level of dehydration. The condition of low temperature and low water content of seeds is conducive to seed conservation.Germination of fresh seeds shows significant variation among populations, howerer, germination of the seeds after storage for one year in room temperature shows no significant variation among populations.High temperature and high relative humidity damages the seeds more severely than high temperature does. In addition, low water content of seeds enable the seeds to be more tolerant to high temperature.The electrical conductivity, dehydrogenase activity and germination percentage have no significant correlation with each other.4. Micropropagation and in vitro conservation，Cotyledonary nodes are a kind of efficient explants. Low salt media are conducive to shoot propagation and root induction.The maximum multiplication rate (20-25 shoots/explant within 4 months) is achieved on quarter-strength Murashige and Skoog (1/4 MS) medium supplemented with 1 mg·L-1 6-benzyladenine (6-BA) and 0.05 mg·L-1 α-naphthaleneacetic acid (NAA).Rooting is promoted by auxins, however, IBA alone or low concentrations of NAA are preferable due to small amount of callus induced. The research has established an efficient protocol for micropropagation of T. doichangensis, and it provides technology support for in vitro conservation of special germplasm of the species.5. Acclimatization，Quercus variabilis, Cyclobalanopsis glaucoides and T. doichangensis belong to the family of Fagaceae, and the natural distribution ranges of the 3 species are decreasing in turn. The research suggests that the ranges of temperature tolerance of the 3 species are decreasing corresponding to their distribution ranges.The high and low semi-lethal temperature of one-year old T. doichangensis is 49.5℃ and -5℃ respectively. It suggests that T. doichangensis has a wide range of basic temperature tolerance. Short-term heat and cold acclimatization cannot expand the range of temperature tolerance. It can be inferred that T. doichangensis may lack induced tolerance to temperature. Under proper conditions, ABA can increase the cold tolerance, and SA can increase the heat tolerance of leaf discs of T. doichangensis.","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&fq=dc.date.issued.year%3A2010&sort_by=2&isNonaffiliated=false&search_type=-1&query1=Clones&order=desc&&fq=dc.project.title_filter%3ATrigonobalanus%5C+doichangensis%5C+is%5C+an%5C+endangered%5C+plant.%5C+In%5C+this%5C+paper%2C%5C+the%5C+megasporogenesis%5C+and%5C+development%5C+of%5C+female%5C+gametophyte%2C%5C+seed%5C+morphological%5C+traits%5C+and%5C+seed%5C+germination%2C%5C+seed%5C+conservation%2C%5C+micropropagation%5C+and%5C+acclimatization%5C+of%5C+this%5C+species%5C+were%5C+studied.%5C+Combined%5C+with%5C+the%5C+published%5C+results%5C+of%5C+cytology%2C%5C+molecular%5C+genetics%5C+and%5C+other%5C+researches%2Cthe%5C+mechanisms%5C+of%5C+extinction%2C%5C+basic%5C+biology%5C+and%5C+technology%5C+of%5C+germplasm%5C+conservation%5C+and%5C+acclimatization%5C+of%5C+T.%5C+doichangensis%5C+were%5C+discussed.%5C+The%5C+main%5C+results%5C+are%5C+summarized%5C+as%5C+follows%5C%3A1.%5C+Megasporogenesis%5C+and%5C+development%5C+of%5C+female%5C+gametophyte%EF%BC%8CStamens%5C+exist%5C+under%5C+the%5C+stigma%5C+of%5C+T.%5C+doichangensis%2C%5C+and%5C+the%5C+pollen%5C+is%5C+aborted%5C+on%5C+the%5C+later%5C+development%5C+stage%5C+of%5C+pistil%2C%5C+therefore%2C%5C+the%5C+pistillate%5C+flower%5C+in%5C+function%5C+is%5C+hermaphrodite%5C+flower%5C+in%5C+morphology.%5C+The%5C+ovule%5C+is%5C+anatropous%2C%5C+bitegmic%5C+and%5C+crassinucellate.%5C+The%5C+primary%5C+archesporium%5C+is%5C+hypodermal%5C+and%5C+single%5C-celled%5C+and%5C+the%5C+sporogenous%5C+cell%5C+of%5C+the%5C+nucellus%5C+functions%5C+directly%5C+as%5C+a%5C+megaspore%5C+mother%5C+cell%5C+which%5C+goes%5C+meiosis%5C+to%5C+form%5C+a%5C+linear%5C+tetrad.%5C+The%5C+chalazal%5C+megaspore%5C+of%5C+the%5C+tetrad%5C+is%5C+functional.%5C+The%5C+development%5C+of%5C+embryo%5C+sac%5C+conforms%5C+to%5C+the%5C+polygonum%5C+type.%5C+There%5C+are%5C+six%5C+ovules%5C+in%5C+the%5C+ovary%5C+of%5C+T.%5C+doichangensis%2C%5C+and%5C+only%5C+one%5C+develops%5C+into%5C+a%5C+seed%5C+in%5C+normal%5C+fruits.%5C+In%5C+the%5C+process%5C+of%5C+megasporogenesis%5C+and%5C+development%5C+of%5C+female%5C+gametophyte%2C%5C+there%5C+are%5C+several%5C+links%5C+of%5C+abortion%2C%5C+and%5C+93.3%25%5C+of%5C+mature%5C+embryo%5C+sacs%5C+is%5C+aborted.2.%5C+Morphological%5C+characters%5C+and%5C+germination%5C+of%5C+seeds%EF%BC%8CMost%5C+of%5C+the%5C+variation%5C+occurred%5C+among%5C+individual%5C+trees%5C+within%5C+populations%5C+in%5C+seed%5C+morphological%5C+traits%5C+%5C%28length%2C%5C+width%5C+and%5C+1000%5C-seed%5C+weight%5C%29%5C+and%5C+germination%5C-related%5C+indices%5C+%5C%28germination%5C+percentage%2C%5C+germination%5C+index%5C+and%5C+vigor%5C+index%5C%29.%5C+In%5C+addition%2C%5C+the%5C+variation%5C+in%5C+percentage%5C+of%5C+well%5C-developed%5C+seeds%5C+among%5C+populations%5C+and%5C+among%5C+individual%5C+trees%5C+within%5C+populations%5C+is%5C+equal%2C%5C+each%5C+accounting%5C+for%5C+48%25.%5C+Each%5C+of%5C+seed%5C+morphological%5C+traits%5C+has%5C+significantly%5C+positive%5C+correlation%5C+with%5C+each%5C+other%5C+%5C%28p%5C+%3C%5C+0.01%5C%29%2C%5C+but%5C+they%5C+have%5C+no%5C+significant%5C+correlation%5C+with%5C+percentage%5C+of%5C+well%5C-developed%5C+seeds%5C+and%5C+germination%5C-related%5C+indices.%5C+In%5C+the%5C+same%5C+batch%5C+of%5C+seeds%5C+of%5C+T.%5C+doichangensis%2C%5C+there%5C+are%5C+light%5C-colored%5C+and%5C+dark%5C-colored%5C+seed%5C+coats%2C%5C+and%5C+development%5C+of%5C+light%5C-colored%5C+seeds%5C+is%5C+significantly%5C+poorer%5C+than%5C+that%5C+of%5C+dark%5C-colored%5C+seeds.The%5C+sensitivity%5C+of%5C+seeds%5C+to%5C+high%5C+temperature%5C+varys%5C+in%5C+different%5C+stages%5C+of%5C+seed%5C+imbibition.%5C+In%5C+each%5C+stage%2C%5C+heat%5C+acclimatization%5C+don%E2%80%99t%5C+increase%5C+germination%5C+percentage%2C%5C+germination%5C+index%5C+and%5C+fresh%5C+weight%5C+of%5C+seedlings.%5C+If%5C+the%5C+distilled%5C+water%5C+is%5C+substituted%5C+by%5C+solution%5C+of%5C+SA%5C+during%5C+seed%5C+imbibition%2C%5C+seed%5C+germination%5C+and%5C+germination%5C+index%5C+after%5C+heat%5C+shock%5C+are%5C+not%5C+significantly%5C+different%5C+from%5C+control%2C%5C+but%5C+they%5C+are%5C+significantly%5C+higher%5C+than%5C+that%5C+of%5C+other%5C+treatments.%5C+Moreover%2C%5C+when%5C+the%5C+seeds%5C+are%5C+treatmented%5C+with%5C+SA%2C%5C+the%5C+fresh%5C+weight%5C+of%5C+seedlings%5C+is%5C+significantly%5C+higher%5C+than%5C+that%5C+of%5C+control%5C+and%5C+other%5C+treatments.3.%5C+Seed%5C+conservation%EF%BC%8CSeeds%5C+of%5C+T.%5C+doichangensis%5C+belong%5C+to%5C+orthodox%5C+seeds%5C+which%5C+can%5C+tolerate%5C+certain%5C+level%5C+of%5C+dehydration.%5C+The%5C+condition%5C+of%5C+low%5C+temperature%5C+and%5C+low%5C+water%5C+content%5C+of%5C+seeds%5C+is%5C+conducive%5C+to%5C+seed%5C+conservation.Germination%5C+of%5C+fresh%5C+seeds%5C+shows%5C+significant%5C+variation%5C+among%5C+populations%2C%5C+howerer%2C%5C+germination%5C+of%5C+the%5C+seeds%5C+after%5C+storage%5C+for%5C+one%5C+year%5C+in%5C+room%5C+temperature%5C+shows%5C+no%5C+significant%5C+variation%5C+among%5C+populations.High%5C+temperature%5C+and%5C+high%5C+relative%5C+humidity%5C+damages%5C+the%5C+seeds%5C+more%5C+severely%5C+than%5C+high%5C+temperature%5C+does.%5C+In%5C+addition%2C%5C+low%5C+water%5C+content%5C+of%5C+seeds%5C+enable%5C+the%5C+seeds%5C+to%5C+be%5C+more%5C+tolerant%5C+to%5C+high%5C+temperature.The%5C+electrical%5C+conductivity%2C%5C+dehydrogenase%5C+activity%5C+and%5C+germination%5C+percentage%5C+have%5C+no%5C+significant%5C+correlation%5C+with%5C+each%5C+other.4.%5C+Micropropagation%5C+and%5C+in%5C+vitro%5C+conservation%EF%BC%8CCotyledonary%5C+nodes%5C+are%5C+a%5C+kind%5C+of%5C+efficient%5C+explants.%5C+Low%5C+salt%5C+media%5C+are%5C+conducive%5C+to%5C+shoot%5C+propagation%5C+and%5C+root%5C+induction.The%5C+maximum%5C+multiplication%5C+rate%5C+%5C%2820%5C-25%5C+shoots%5C%2Fexplant%5C+within%5C+4%5C+months%5C%29%5C+is%5C+achieved%5C+on%5C+quarter%5C-strength%5C+Murashige%5C+and%5C+Skoog%5C+%5C%281%5C%2F4%5C+MS%5C%29%5C+medium%5C+supplemented%5C+with%5C+1%5C+mg%C2%B7L%5C-1%5C+6%5C-benzyladenine%5C+%5C%286%5C-BA%5C%29%5C+and%5C+0.05%5C+mg%C2%B7L%5C-1%5C+%CE%B1%5C-naphthaleneacetic%5C+acid%5C+%5C%28NAA%5C%29.Rooting%5C+is%5C+promoted%5C+by%5C+auxins%2C%5C+however%2C%5C+IBA%5C+alone%5C+or%5C+low%5C+concentrations%5C+of%5C+NAA%5C+are%5C+preferable%5C+due%5C+to%5C+small%5C+amount%5C+of%5C+callus%5C+induced.%5C+The%5C+research%5C+has%5C+established%5C+an%5C+efficient%5C+protocol%5C+for%5C+micropropagation%5C+of%5C+T.%5C+doichangensis%2C%5C+and%5C+it%5C+provides%5C+technology%5C+support%5C+for%5C+in%5C+vitro%5C+conservation%5C+of%5C+special%5C+germplasm%5C+of%5C+the%5C+species.5.%5C+Acclimatization%EF%BC%8CQuercus%5C+variabilis%2C%5C+Cyclobalanopsis%5C+glaucoides%5C+and%5C+T.%5C+doichangensis%5C+belong%5C+to%5C+the%5C+family%5C+of%5C+Fagaceae%2C%5C+and%5C+the%5C+natural%5C+distribution%5C+ranges%5C+of%5C+the%5C+3%5C+species%5C+are%5C+decreasing%5C+in%5C+turn.%5C+The%5C+research%5C+suggests%5C+that%5C+the%5C+ranges%5C+of%5C+temperature%5C+tolerance%5C+of%5C+the%5C+3%5C+species%5C+are%5C+decreasing%5C+corresponding%5C+to%5C+their%5C+distribution%5C+ranges.The%5C+high%5C+and%5C+low%5C+semi%5C-lethal%5C+temperature%5C+of%5C+one%5C-year%5C+old%5C+T.%5C+doichangensis%5C+is%5C+49.5%E2%84%83%5C+and%5C+%5C-5%E2%84%83%5C+respectively.%5C+It%5C+suggests%5C+that%5C+T.%5C+doichangensis%5C+has%5C+a%5C+wide%5C+range%5C+of%5C+basic%5C+temperature%5C+tolerance.%5C+Short%5C-term%5C+heat%5C+and%5C+cold%5C+acclimatization%5C+cannot%5C+expand%5C+the%5C+range%5C+of%5C+temperature%5C+tolerance.%5C+It%5C+can%5C+be%5C+inferred%5C+that%5C+T.%5C+doichangensis%5C+may%5C+lack%5C+induced%5C+tolerance%5C+to%5C+temperature.%5C+Under%5C+proper%5C+conditions%2C%5C+ABA%5C+can%5C+increase%5C+the%5C+cold%5C+tolerance%2C%5C+and%5C+SA%5C+can%5C+increase%5C+the%5C+heat%5C+tolerance%5C+of%5C+leaf%5C+discs%5C+of%5C+T.%5C+doichangensis."},{"jsname":"lastIndexed","jscount":"2024-06-27"}],"资助项目","dc.project.title_filter")'>
Aconitum c... [1]
During a f... [1]
In Chapter... [1]
Natural hy... [1]
Orchidacea... [1]
Pediculari... [1]
更多...
收录类别
SCI [11]
CSCD [1]
资助机构
Chinese Ac... [1]
Germplasm ... [1]
Ministry o... [1]
National B... [1]
National N... [1]
National N... [1]
更多...
×
知识图谱
KIB OpenIR
开始提交
已提交作品
待认领作品
已认领作品
未提交全文
收藏管理
QQ客服
官方微博
反馈留言
浏览/检索结果:
共27条，第1-10条
帮助
限定条件
发表日期：2010
已选(
0
)
清除
条数/页：
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
排序方式：
请选择
作者升序
作者降序
WOS被引频次升序
WOS被引频次降序
期刊影响因子升序
期刊影响因子降序
发表日期升序
发表日期降序
题名升序
题名降序
提交时间升序
提交时间降序
Chloroplast phylogeny and phylogeography of Stellera chamaejasme on the Qinghai-Tibet Plateau and in adjacent regions
期刊论文
MOLECULAR PHYLOGENETICS AND EVOLUTION, 2010, 卷号: 57, 期号: 3, 页码: 1162-1172
作者:
Zhang, Yong-Hong
;
Volis, Sergei
;
Sun, Hang
Adobe PDF(1829Kb)
|
收藏
|
浏览/下载：635/315
|
提交时间：2011/12/20
Stellera Chamaejasme
Phylogeny
Phylogeography
Qinghai-tibet Plateau
Refugium
Post-glacial Expansion
Functional conservation of the plant EMBRYONIC FLOWER2 gene between bamboo and Arabidopsis
期刊论文
BIOTECHNOLOGY LETTERS, 2010, 卷号: 32, 期号: 12, 页码: 1961-1968
作者:
Xu, Hong
;
Chen, Ling-Jing
;
Qu, Li-Jia
;
Gu, Hong-Ya
;
Li, De-Zhu
Adobe PDF(523Kb)
|
收藏
|
浏览/下载：340/146
|
提交时间：2012/06/12
Anti-sense
Bamboo Emf2
Rapid Amplification Of Cdna Ends
Real-time Pcr
Rescue
蓝果杜鹃与马缨花杜鹃的自然杂交研究
学位论文
: 中国科学院研究生院, 2010
马永鹏
Adobe PDF(10926Kb)
|
收藏
|
浏览/下载：446/39
|
提交时间：2013/01/28
橐吾属的杂交物种形成研究—莲叶橐吾和穗序橐吾的自然杂交
学位论文
: 中国科学院研究生院, 2010
余姣君
Adobe PDF(1806Kb)
|
收藏
|
浏览/下载：346/25
|
提交时间：2013/01/28
三种欺骗性兰花的传粉生态学
学位论文
: 中国科学院研究生院, 2010
任宗昕
Adobe PDF(6910Kb)
|
收藏
|
浏览/下载：515/43
|
提交时间：2013/01/28
马先蒿属斗叶组的分子系统学——兼大王马先蒿复合群的分类学修订
学位论文
: 中国科学院研究生院, 2010
黄盼辉
Adobe PDF(8049Kb)
|
收藏
|
浏览/下载：304/17
|
提交时间：2013/01/28
乌头多倍体复合群的分子谱系地理学--兼论川乌的起源
学位论文
, 北京: 中国科学院研究生院, 2010
作者:
何俊
Adobe PDF(14623Kb)
|
收藏
|
浏览/下载：578/29
|
提交时间：2013/01/28
UNUSUAL PATTERNS OF HYBRIDIZATION INVOLVING A NARROW ENDEMIC RHODODENDRON SPECIES (ERICACEAE) IN YUNNAN, CHINA
期刊论文
AMERICAN JOURNAL OF BOTANY, 2010, 卷号: 97, 期号: 10, 页码: 1749-1757
作者:
Ma, Yongpeng
;
Milne, Richard I.
;
Zhang, Changqin
;
Yang, Junbo
Adobe PDF(840Kb)
|
收藏
|
浏览/下载：287/85
|
提交时间：2012/01/05
Ericaceae
Habitat Disturbance
Hybrid Zone
Narrow Endemic Species
Rhododendron Cyanocarpum
Rhododendron Delavayi
Natural Hybridization between Rhododendron delavayi and R-cyanocarpum (Ericaceae), from Morphological, Molecular and Reproductive Evidence
期刊论文
JOURNAL OF INTEGRATIVE PLANT BIOLOGY, 2010, 卷号: 52, 期号: 9, 页码: 844-851
作者:
Ma, Yong-Peng
;
Zhang, Chang-Qin
;
Zhang, Jing-Li
;
Yang, Jun-Bo
Adobe PDF(337Kb)
|
收藏
|
浏览/下载：296/103
|
提交时间：2011/12/20
Photosynthetic characteristics of two alpine flowers, Meconopsis integrifolia and Primula sinopurpurea
期刊论文
JOURNAL OF HORTICULTURAL SCIENCE & BIOTECHNOLOGY, 2010, 卷号: 85, 期号: 4, 页码: 335-340
作者:
Zhang, Shi-Bao
;
Chang, Wei
;
Hu, Hong
Adobe PDF(123Kb)
|
收藏
|
浏览/下载：446/102
|
提交时间：2012/07/18
