| Precise structures and anti-intrinsic tenase complex activity of three fucosylated glycosaminoglycans and their fragments | |
Cai, Ying1,2,3; Yang, Wenjiao2,3; Li, Xiaomei4; Zhou, Lutan2,3; Wang, Zhongjuan5; Lin, Lisha2,3; Chen, Dingyuan2,3 ; Zhao, Longyan6; Li, Zhongkun5; Liu, Shubai2; Yin, Ronghua2; Zuo, Zhili2; Gao, Na6; Zhao, Jinhua2,6
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| 通讯作者 | Yin, Ronghua(yinronghua@mail.kib.ac.cn) ; Zuo, Zhili(zuozhili@mail.kib.ac.cn) ; Gao, Na(gn2008.happy@163.com) |
| 2019-11-15 | |
| 发表期刊 | CARBOHYDRATE POLYMERS
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| ISSN | 0144-8617 |
| 卷号 | 224页码:10 |
| 摘要 | Fucosylated glycosaminoglycan (FG), a glycosaminoglycan derivative containing distinct sulfated fucose (FucS) branches, displays potent anticoagulant activity by inhibiting the intrinsic tenase complex (iXase). Herein, AmFG, SvFG and HaFG from three species of sea cucumbers were isolated and depolymerized by beta-eliminative cleavage. Three series of fragments, A1-A4, S1-S4 and H1-H4, were purified from the depolymerized FGs. Based on structural analysis of these fragments, three FGs were deduced as -{-> 4)-[L-FucS-alpha(1 -> 3)]-D-GlcA-beta(1 -> 3)-D-GalNAc(4S6S)-beta(1}(n-). The structures differed in sulfation types of FucS, namely, most of FucS in AmFG was Fuc(3S4S), but the FucS in SvFG was Fuc(2S4S), while the FucS in HaFG was Fuc(3S4S), Fuc(2S4S) and Fuc(4S). However, all FucS branches attached to C-3 of GlcA as monosaccharides. Anticoagulant and anti-iXase assays showed the octasaccharide is the minimum fragment for potent anticoagulant activity via anti-iXase irrespective of FucS types. Among FG fragments with same degree of polymerization, oligosaccharides containing Fuc(2S4S) had more potent anti-iXase activity. |
| 关键词 | Fucosylated glycosaminoglycan Oligosaccharide Chemical structure iXase |
| DOI | 10.1016/j.carbpol.2019.115146 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000484001200019 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.kib.ac.cn/handle/151853/68757 |
| 专题 | 植物化学与西部植物资源持续利用国家重点实验室 |
| 通讯作者 | Yin, Ronghua; Zuo, Zhili; Gao, Na |
| 作者单位 | 1.Yunnan Univ, Sch Life Sci, Kunming 650500, Yunnan, Peoples R China 2.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chuxiong Med Coll, Chuxiong 675005, Peoples R China 5.Kunming Med Univ, Yanan Hosp, Dept Pharm, Kunming 650051, Yunnan, Peoples R China 6.South Cent Univ Nationalities, Sch Pharmaceut Sci, Wuhan 430074, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cai, Ying,Yang, Wenjiao,Li, Xiaomei,et al. Precise structures and anti-intrinsic tenase complex activity of three fucosylated glycosaminoglycans and their fragments[J]. CARBOHYDRATE POLYMERS,2019,224:10. |
| APA | Cai, Ying.,Yang, Wenjiao.,Li, Xiaomei.,Zhou, Lutan.,Wang, Zhongjuan.,...&Zhao, Jinhua.(2019).Precise structures and anti-intrinsic tenase complex activity of three fucosylated glycosaminoglycans and their fragments.CARBOHYDRATE POLYMERS,224,10. |
| MLA | Cai, Ying,et al."Precise structures and anti-intrinsic tenase complex activity of three fucosylated glycosaminoglycans and their fragments".CARBOHYDRATE POLYMERS 224(2019):10. |
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