Nonasaccharide Inhibits Intrinsic Factor Xase Complex by Binding to Factor IXa and Disrupting Factor IXa-Factor VIIIa Interactions

doi:10.1055/s-0039-1681047

	Nonasaccharide Inhibits Intrinsic Factor Xase Complex by Binding to Factor IXa and Disrupting Factor IXa-Factor VIIIa Interactions
	Xiao, Chuang1,2,3,4 ; Zhao, Longyan1,2,5 ; Gao, Na 1,2,5; Wu, Mingyi 1; Zhao, Jinhua1
通讯作者	Zhao, Jinhua(zhao.jinhua@yahoo.com)
	2019-05-01
发表期刊	THROMBOSIS AND HAEMOSTASIS
ISSN	0340-6245
卷号	119 期号:5 页码:705-715
摘要	A nonasaccharide (FG9) derived from natural fucosylated glycosaminoglycan (FG) is identified as a selective intrinsic factor Xase complex (FIXa-FVIIIa-Ca2+-phospholipid, FXase) inhibitor that possesses potential inhibition of venous thrombus in rats and shows negligible bleeding risk. The mechanism and molecular target of the nonasaccharide for intrinsic FXase inhibition were systematically investigated and compared with low molecular weight heparin (LMWH). Our results showed that FG9 dose-dependently inhibited FX activation by intrinsic FXase complex in a noncompetitive inhibition pattern, where the apparent affinity for FG9 was approximately 1.8-fold higher than that for LMWH. FG9 displayed no inhibitory effect on the activity of FIXa/phospholipid, and did not affect the decay rate of FVIIIa activity. FG9 reduced the apparent affinity of FIXa for FVIIIa in a dose-dependent manner, and accelerated the decay of intrinsic FXase complex activity. FG9 bound to FIXa with high affinity and the FIXa binding sites of FG9 were overlapped with that of LMWH, and the ability of FG-derived oligosaccharides to bind FIXa required the minimum 9 degrees of polymerization. FG9 derivatives were prepared and their structures were confirmed by one-dimensional/two-dimensional nuclear magnetic resonance. Structure-activity relationship studies showed that carboxy reduction significantly weakened its anti-FXase activity and binding affinity to FIXa, while the effects of carboxyl ethyl esterification and deacetylation were relatively weaker. Overall, our results suggest that the nonasaccharide FG9 strongly inhibits intrinsic FXase complex activity via binding to FIXa and disrupting FIXa-FVIIIa interactions, and the free carboxyl groups of FG9 are required for its potent anti-FXase activity.
关键词	nonasaccharide from fucosylated glycosaminoglycan intrinsic factor Xase complex factor IXa anticoagulant
DOI	10.1055/s-0039-1681047
收录类别	SCI
语种	英语
WOS记录号	WOS:000467408100005
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/66867
专题	植物化学与西部植物资源持续利用国家重点实验室
通讯作者	Zhao, Jinhua
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, 132 Lanhei Rd, Kunming 650201, Yunnan, Peoples R China 2.Univ Chinese Acad Sci, Coll Life Sci, Beijing, Peoples R China 3.Kunming Med Univ, Sch Pharmaceut Sci, Kunming, Yunnan, Peoples R China 4.Kunming Med Univ, Yunnan Key Lab Pharmacol Nat Prod, Kunming, Yunnan, Peoples R China 5.South Cent Univ Nationalities, Sch Pharmaceut Sci, Wuhan, Hubei, Peoples R China
推荐引用方式 GB/T 7714	Xiao, Chuang,Zhao, Longyan,Gao, Na,et al. Nonasaccharide Inhibits Intrinsic Factor Xase Complex by Binding to Factor IXa and Disrupting Factor IXa-Factor VIIIa Interactions[J]. THROMBOSIS AND HAEMOSTASIS,2019,119(5):705-715.
APA	Xiao, Chuang,Zhao, Longyan,Gao, Na,Wu, Mingyi,&Zhao, Jinhua.(2019).Nonasaccharide Inhibits Intrinsic Factor Xase Complex by Binding to Factor IXa and Disrupting Factor IXa-Factor VIIIa Interactions.THROMBOSIS AND HAEMOSTASIS,119(5),705-715.
MLA	Xiao, Chuang,et al."Nonasaccharide Inhibits Intrinsic Factor Xase Complex by Binding to Factor IXa and Disrupting Factor IXa-Factor VIIIa Interactions".THROMBOSIS AND HAEMOSTASIS 119.5(2019):705-715.

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