Pyrocincholic acid 3beta-O-beta-D-quinovopyranosyl-28-O-beta-D-glucopyranoside suppresses adipogenesis and regulates lipid metabolism in 3T3-L1 adipocytes.

	Pyrocincholic acid 3beta-O-beta-D-quinovopyranosyl-28-O-beta-D-glucopyranoside suppresses adipogenesis and regulates lipid metabolism in 3T3-L1 adipocytes.
	Peng, Li; Lu, Yanting; Xu, Yuhui; Hu, Jing; Wang, Fang; Zhang, Yumei; Xiong, Wenyong
	2017
发表期刊	Natural products and bioprospecting
ISSN	2192-2195
卷号	7 期号:3 页码:225-234
摘要	Obesity is crucially involved in many metabolic diseases, such as type 2 diabetes, cardiovascular disease and cancer. Regulating the number or size of adipocytes has been suggested to be a potential treatment for obesity. In this study, we investigated the effect of pyrocincholic acid 3beta-O-beta-D-quinovopyranosyl-28-O-beta-D-glucopyranoside (PAQG), a 27-nor-oleanolic acid saponin extracted from Metadina trichotoma, on adipogenesis and lipid metabolism in 3T3-L1 adipocytes. The 3T3-L1 pre-adipocytes were incubated with vehicle or PAQG for 6days in differentiation process. PAQG significantly reduced the adipogenesis, adiponectin secretion and the expression level of key transcription factors related to adipogenesis, such as PPARgamma, C/EBPbeta, C/EBPalpha, and FABP4. Moreover, PAQG increased the levels of FFA and glycerol in medium and reduced TG level in mature adipocytes. Interestingly, PAQG not only promoted the activation of AMPK and genes involved in fatty oxidation including PDK4 and CPT1a, but also inhibited those genes involved in fatty acid biosynthesis, such as SREBP1c, FAS, ACCalpha and SCD1. In conclusion, PAQG inhibits the differentiation and regulates lipid metabolism of 3T3-L1 cells via AMPK pathway, suggesting that PAQG may be a novel and promising natural product for the treatment of obesity and hyperlipidemia.
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/64842
专题	植物化学与西部植物资源持续利用国家重点实验室
推荐引用方式 GB/T 7714	Peng, Li,Lu, Yanting,Xu, Yuhui,et al. Pyrocincholic acid 3beta-O-beta-D-quinovopyranosyl-28-O-beta-D-glucopyranoside suppresses adipogenesis and regulates lipid metabolism in 3T3-L1 adipocytes.[J]. Natural products and bioprospecting,2017,7(3):225-234.
APA	Peng, Li.,Lu, Yanting.,Xu, Yuhui.,Hu, Jing.,Wang, Fang.,...&Xiong, Wenyong.(2017).Pyrocincholic acid 3beta-O-beta-D-quinovopyranosyl-28-O-beta-D-glucopyranoside suppresses adipogenesis and regulates lipid metabolism in 3T3-L1 adipocytes..Natural products and bioprospecting,7(3),225-234.
MLA	Peng, Li,et al."Pyrocincholic acid 3beta-O-beta-D-quinovopyranosyl-28-O-beta-D-glucopyranoside suppresses adipogenesis and regulates lipid metabolism in 3T3-L1 adipocytes.".Natural products and bioprospecting 7.3(2017):225-234.