Insecticidal and Acetylcholinesterase Inhibition Activity of Veratrum nigrum Alkaloidal Extract against the German Cockroach (Blattella germanica)

	Insecticidal and Acetylcholinesterase Inhibition Activity of Veratrum nigrum Alkaloidal Extract against the German Cockroach (Blattella germanica)
	Cai, Xianghai1 ; Li, Qingfeng 2; Xiao, Lei 3; Lu, Hailiang 3; Tang, Jian 3; Huang, Jianbo 2; Yuan, Jianzhong 2
	2018-12-01
发表期刊	JOURNAL OF ARTHROPOD-BORNE DISEASES
ISSN	2322-1984
卷号	12 期号:4 页码:414-420
摘要	Background: Veratrum nigrum (Liliaceae) is perennial medicinal plant widely used to treat various conditions. To determine its insecticidal properties against the German cockroach (Blattella germanica), several laboratory tests were carried out. Methods: A 4kg dry sample of V. nigrum root was purchased from the medicinal material market in Yunnan Province in 2015, China. In contact toxicity tests, V. nigrum alkaloidal extract was topically applied to the abdomen of cockroaches using a micro-applicator. In vitro acetylcholinesterase (AChE) activity tests were performed using a modified Ellman method. Results: Veratrum nigrum alkaloidal extract was toxic to male adults and 4th nymphs cockroaches, with median lethal dose (LD50) values of 14.90 mu g/insect, 14.21 mu g/insect for adults and 41.45 mu g/insect, 39.01 mu g/insect for 4th nymphs after 24h and 48h exposure, respectively. There was a significant difference between adults and nymphs in terms of tolerance to V. nigrum alkaloidal extract. There was no significant difference in mortalities at 24h and 48h, the lethal effect of V. nigrum alkaloidal extract on German cockroach was quick. AChE activity tests showed that V. nigrum alkaloidal extract had an excellent inhibitory effect on AChE: inhibition in the 4th nymphs and male adults had 50% inhibiting concentration (IC50) values of 3.56mg/ml and 5.78mg/ml respectively. The inhibitory effect of AChE activity was positively correlated with inhibitory time (0-20min), at a concentration of 1mg/ml, inhibition of nymph and adult AChE activity had 50% inhibiting time (IT50) values of 8.34min and 16.75min, respectively. Conclusion: V. nigrum may be explored as a potential natural insecticide for control of the German cockroach.
关键词	Insecticidal activity Acetylcholinesterase inhibition Veratrum nigrum Medicinal plant German cockroach
收录类别	SCI
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/64453
专题	植物化学与西部植物资源持续利用国家重点实验室
通讯作者	Yuan, Jianzhong
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Shanghai, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Shanghai, Peoples R China 3.Infinitus China Ltd Co, Guangzhou, Guangdong, Peoples R China
推荐引用方式 GB/T 7714	Cai, Xianghai,Li, Qingfeng,Xiao, Lei,et al. Insecticidal and Acetylcholinesterase Inhibition Activity of Veratrum nigrum Alkaloidal Extract against the German Cockroach (Blattella germanica)[J]. JOURNAL OF ARTHROPOD-BORNE DISEASES,2018,12(4):414-420.
APA	Cai, Xianghai.,Li, Qingfeng.,Xiao, Lei.,Lu, Hailiang.,Tang, Jian.,...&Yuan, Jianzhong.(2018).Insecticidal and Acetylcholinesterase Inhibition Activity of Veratrum nigrum Alkaloidal Extract against the German Cockroach (Blattella germanica).JOURNAL OF ARTHROPOD-BORNE DISEASES,12(4),414-420.
MLA	Cai, Xianghai,et al."Insecticidal and Acetylcholinesterase Inhibition Activity of Veratrum nigrum Alkaloidal Extract against the German Cockroach (Blattella germanica)".JOURNAL OF ARTHROPOD-BORNE DISEASES 12.4(2018):414-420.