Oligosaccharides from depolymerized fucosylated glycosaminoglycan: Structures and minimum size for intrinsic factor Xase complex inhibition
Yin, Ronghua1,2; Zhou, Lutan1,2; Gao, Na1; Li, Zi1; Zhao, Longyan1; Shang, Feineng1; Wu, Mingyi1; Zhao, Jinhua1
2018-09-07
发表期刊JOURNAL OF BIOLOGICAL CHEMISTRY
ISSN0021-9258
卷号293期号:36页码:14089-14099
摘要Fucosylated glycosaminoglycan (FG), a structurally complex glycosaminoglycan found up to now exclusively in sea cucumbers, has distinct anticoagulant properties, notably a strong inhibitory activity of intrinsic factor Xase complex (FXase). Knowledge of the FG structures could facilitate the development of a clinically effective intrinsic FXase inhibitor for anticoagulant drugs. Here, a new fucosylated glycosaminoglycan was obtained from the widely traded sea cucumber Bohadschia argus. The precise structure was deduced as {4)-[l-Fuc3S4S--(13)-]-d-GlcA--(13)-d-GalNAc4S6S--(1} through analysis of its chemical properties and homogeneous oligosaccharides purified from its -eliminative depolymerized products. The B. argus FG with mostly 3,4-di-O-sulfated fucoses expands our knowledge on FG structural types. This -elimination process, producing oligosaccharides with well-defined structures, is a powerful tool for analyzing the structure of complex FGs. Among these oligosaccharides, an octasaccharide displayed potent FXase inhibitory activity. Compared with oligosaccharides with various degrees of polymerization (3n and 3n - 1), our analyses reveal that the purified octasaccharide is the minimum structural unit responsible for the potent selective FXase inhibition, because the d-talitol in the nonsaccharide is unnecessary. The octasaccharide with 2,4-di-O-sulfated fucoses is more potent than that of one with 3,4-di-O-sulfated fucoses. Thus, sulfation patterns can play an important role in the inhibition of intrinsic factor Xase complex.
关键词natural product carbohydrate chemistry carbohydrate structure chemical modification drug discovery oligosaccharide thrombosis
资助信息National Natural Science Foundation of China; Yunnan Provincial Science and Technology Department; Kunming Institute of Botany; Chinese Academy of Sciences Youth Innovation Promotion Association
DOI10.1074/jbc.RA118.003809
语种英语
资助项目National Natural Science Foundation of China[81673330] ; National Natural Science Foundation of China[81773737] ; Yunnan Provincial Science and Technology Department[2016FA050] ; Yunnan Provincial Science and Technology Department[2010CI116] ; Kunming Institute of Botany[KIB2017011] ; Chinese Academy of Sciences Youth Innovation Promotion Association[2017435]
WOS研究方向Biochemistry & Molecular Biology
WOS类目Biochemistry & Molecular Biology
WOS记录号WOS:000444070700030
出版者AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
引用统计
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/62298
专题植物化学与西部植物资源持续利用国家重点实验室
通讯作者Wu, Mingyi; Zhao, Jinhua
作者单位1.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
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Yin, Ronghua,Zhou, Lutan,Gao, Na,et al. Oligosaccharides from depolymerized fucosylated glycosaminoglycan: Structures and minimum size for intrinsic factor Xase complex inhibition[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2018,293(36):14089-14099.
APA Yin, Ronghua.,Zhou, Lutan.,Gao, Na.,Li, Zi.,Zhao, Longyan.,...&Zhao, Jinhua.(2018).Oligosaccharides from depolymerized fucosylated glycosaminoglycan: Structures and minimum size for intrinsic factor Xase complex inhibition.JOURNAL OF BIOLOGICAL CHEMISTRY,293(36),14089-14099.
MLA Yin, Ronghua,et al."Oligosaccharides from depolymerized fucosylated glycosaminoglycan: Structures and minimum size for intrinsic factor Xase complex inhibition".JOURNAL OF BIOLOGICAL CHEMISTRY 293.36(2018):14089-14099.
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