Oligosaccharides from depolymerized fucosylated glycosaminoglycan: Structures and minimum size for intrinsic factor Xase complex inhibition
Yin, Ronghua1,2; Zhou, Lutan1,2; Gao, Na1; Li, Zi1; Zhao, Longyan1; Shang, Feineng1; Wu, Mingyi1; Zhao, Jinhua1
2018-09-07
Source PublicationJOURNAL OF BIOLOGICAL CHEMISTRY
ISSN0021-9258
Volume293Issue:36Pages:14089-14099
Abstract

Fucosylated glycosaminoglycan (FG), a structurally complex glycosaminoglycan found up to now exclusively in sea cucumbers, has distinct anticoagulant properties, notably a strong inhibitory activity of intrinsic factor Xase complex (FXase). Knowledge of the FG structures could facilitate the development of a clinically effective intrinsic FXase inhibitor for anticoagulant drugs. Here, a new fucosylated glycosaminoglycan was obtained from the widely traded sea cucumber Bohadschia argus. The precise structure was deduced as {4)-[l-Fuc3S4S--(13)-]-d-GlcA--(13)-d-GalNAc4S6S--(1} through analysis of its chemical properties and homogeneous oligosaccharides purified from its -eliminative depolymerized products. The B. argus FG with mostly 3,4-di-O-sulfated fucoses expands our knowledge on FG structural types. This -elimination process, producing oligosaccharides with well-defined structures, is a powerful tool for analyzing the structure of complex FGs. Among these oligosaccharides, an octasaccharide displayed potent FXase inhibitory activity. Compared with oligosaccharides with various degrees of polymerization (3n and 3n - 1), our analyses reveal that the purified octasaccharide is the minimum structural unit responsible for the potent selective FXase inhibition, because the d-talitol in the nonsaccharide is unnecessary. The octasaccharide with 2,4-di-O-sulfated fucoses is more potent than that of one with 3,4-di-O-sulfated fucoses. Thus, sulfation patterns can play an important role in the inhibition of intrinsic factor Xase complex.

KeywordNatural Product Carbohydrate Chemistry Carbohydrate Structure Chemical Modification Drug Discovery Oligosaccharide Thrombosis
DOI10.1074/jbc.RA118.003809
Language英语
WOS Research AreaBiochemistry & Molecular Biology
WOS SubjectBiochemistry & Molecular Biology
WOS IDWOS:000444070700030
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Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/62298
Collection植物化学与西部植物资源持续利用国家重点实验室
Corresponding AuthorWu, Mingyi; Zhao, Jinhua
Affiliation1.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Yin, Ronghua,Zhou, Lutan,Gao, Na,et al. Oligosaccharides from depolymerized fucosylated glycosaminoglycan: Structures and minimum size for intrinsic factor Xase complex inhibition[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2018,293(36):14089-14099.
APA Yin, Ronghua.,Zhou, Lutan.,Gao, Na.,Li, Zi.,Zhao, Longyan.,...&Zhao, Jinhua.(2018).Oligosaccharides from depolymerized fucosylated glycosaminoglycan: Structures and minimum size for intrinsic factor Xase complex inhibition.JOURNAL OF BIOLOGICAL CHEMISTRY,293(36),14089-14099.
MLA Yin, Ronghua,et al."Oligosaccharides from depolymerized fucosylated glycosaminoglycan: Structures and minimum size for intrinsic factor Xase complex inhibition".JOURNAL OF BIOLOGICAL CHEMISTRY 293.36(2018):14089-14099.
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