Longikaurin A, a natural ent-kaurane, suppresses stemness in nasopharyngeal carcinoma cells
Yuan,Yan; Du,Yong; Hu,Xiao-Ye; Liu,Mei-Yuan; Du,Ji-Ke; Liu,Xue-Min; Yu,Hong-En; Wang,Tian-Zhu; Pu,Jian-Xin; Zhong,Qian; Zou,Qing-Feng
2017
发表期刊ONCOLOGY LETTERS
ISSN1792-1074
卷号13期号:3页码:1672-1680
摘要

Cancer stem cells (CSCs) are a small proportion of tumor cells that may be responsible for tumor metastasis and recurrence. Our recent research indicated that longikaurin A (LK-A) exhibited anti-tumor activity in nasopharyngeal carcinoma (NPC) both in vitro and in vivo. Here, we further investigated whether LK-A could suppress the stemness of NPC cells. Sphere formation assay was used to assess the self-renewal ability of the cells treated with LK-A. Side population (SP) was determined by flow cytometry to measure the influence of LK-A on NPC SPs. The expression of the c-myc and fibronectin was detected by western blotting. The cytotoxicity of LK-A in combination with cisplatin to NPC cells was determined by MTT assay. Colony formation assay was used to verify whether LK-A could sensitize NPC cells to radiation and reverse the radiotherapy resistance. In the present study, we found that LK-A reduced the number and size of spheroid formation and decreased the SP cell percentage of the S18 cell line at a low concentration. Furthermore, LK-A treatment downregulated the expression of c-myc and fibronectin in NPC cell lines. Moreover, LK-A could significantly enhance the chemotherapeutic and radiotherapeutic sensitivity of NPC cell lines and reverse acquired radiotherapy resistance of Sune2-IR. Our data revealed that LK-A could suppress the stemness of NPC cells and may enhance the efficacy of radiotherapy and chemotherapy.

关键词Side Population C-myc Cancer Expression Radioresistance Identification Subpopulation Combination Pathway Domain Longikaurin a Nasopharyngeal Carcinoma Cancer Stem Cells
收录类别SCI
语种英语
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文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/54960
专题植物化学与西部植物资源持续利用国家重点实验室
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Yuan,Yan,Du,Yong,Hu,Xiao-Ye,et al. Longikaurin A, a natural ent-kaurane, suppresses stemness in nasopharyngeal carcinoma cells[J]. ONCOLOGY LETTERS,2017,13(3):1672-1680.
APA Yuan,Yan.,Du,Yong.,Hu,Xiao-Ye.,Liu,Mei-Yuan.,Du,Ji-Ke.,...&Zou,Qing-Feng.(2017).Longikaurin A, a natural ent-kaurane, suppresses stemness in nasopharyngeal carcinoma cells.ONCOLOGY LETTERS,13(3),1672-1680.
MLA Yuan,Yan,et al."Longikaurin A, a natural ent-kaurane, suppresses stemness in nasopharyngeal carcinoma cells".ONCOLOGY LETTERS 13.3(2017):1672-1680.
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