| Using Ultra-Performance Liquid Chromatography Quadrupole Time of Flight Mass Spectrometry-Based Chemometrics for the Identification of Anti-angiogenic Biflavonoids from Edible Garcinia Species | |
| Li, Ping1,2; Yue, Grace Gar-Lee3,4; Kwok, Hin-Fai3,4; Long, Chun-lin1,5; Lau, Clara Bik-San3,4; Kennelly, Edward J.1,6,7 | |
| 2017-09-27 | |
| 发表期刊 | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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| ISSN | 0021-8561 |
| 卷号 | 65期号:38页码:8348-8355 |
| 摘要 | Garcinia xanthochymus fruits are edible and also used in traditional medicine. Our previous work showed that the isolated natural products from G. xanthochymus fruits have displayed antioxidant activity and cytotoxicity in the colon cancer cells. In this study, we developed a strategy to correlate a zebrafish angiogenesis assay with ultra-performance liquid chromatography quadrupole time of flight mass spectrometry-based chemometric analysis to identify potential anti-angiogenic activity compounds from G. xanthochymus fruits. Primary bioactivity results showed that the methanolic extracts from aril and pericarp but not from seed have significant inhibitory effects on the growth of subintestinal vessels (SIVs) in zebrafish embryos. A total of 13 markers, including benzophenones and biflavonoids, were predicted by untargeted principal component analysis and orthogonal partial least squares discriminate analysis, which were tentatively identified as priority markers for the bioactivity related in aril and pericarp. Amentoflavone, a biflavonoid, has been found to significantly inhibit the growth of SIVs at 10 and 20 mu M and downregulate the expressions of Angpt2 and Tie2 genes of zebrafish embryos. Furthermore, seven biflavonoids, volkensiflavone, fukugetin, fukugeside, GB 1a, GB 1a glucoside, GB 2a, and GB 2a glucoside, isolated from Garcinia species were evaluated for their structure-activity relationship using the zebrafish model. Only fukugetin, which was previously shown to be anticancer, was active in inhibiting the SW growth. In this report, both amentoflavone and fukugetin, for the first time, displayed anti-angiogenic effects on zebrafish, thus demonstrating an effective and rapid strategy to identify natural products for anti-angiogenesis activity. |
| 关键词 | Anti-angiogenesis Garcinia Xanthochymus Uplc-qtof-ms Pca Biflavonoids |
| 学科领域 | Agriculture, Multidisciplinary ; Chemistry, Applied ; Food Science & Technology |
| DOI | 10.1021/acs.jafc.7b02867 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000412042800008 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.kib.ac.cn/handle/151853/54878 |
| 专题 | 资源植物与生物技术所级重点实验室 |
| 作者单位 | 1.Minzu Univ China, Coll Life & Environm Sci, Beijing 100081, Peoples R China 2.South China Agr Univ, Key Lab Agroenvironm Trop, Minist Agr, Guangzhou 510642, Guangdong, Peoples R China 3.Chinese Univ Hong Kong, Inst Chinese Med, Shatin, Hong Kong, Peoples R China 4.Chinese Univ Hong Kong, State Key Lab Phytochem & Plant Resources West Ch, Shatin, Hong Kong, Peoples R China 5.Chinese Acad Sci, Kunming Inst Bot, Kunming 650201, Yunnan, Peoples R China 6.CUNY, Lehman Coll, Dept Biol Sci, New York, NY 10468 USA 7.CUNY, Grad Ctr, New York, NY 10468 USA |
| 推荐引用方式 GB/T 7714 | Li, Ping,Yue, Grace Gar-Lee,Kwok, Hin-Fai,et al. Using Ultra-Performance Liquid Chromatography Quadrupole Time of Flight Mass Spectrometry-Based Chemometrics for the Identification of Anti-angiogenic Biflavonoids from Edible Garcinia Species[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2017,65(38):8348-8355. |
| APA | Li, Ping,Yue, Grace Gar-Lee,Kwok, Hin-Fai,Long, Chun-lin,Lau, Clara Bik-San,&Kennelly, Edward J..(2017).Using Ultra-Performance Liquid Chromatography Quadrupole Time of Flight Mass Spectrometry-Based Chemometrics for the Identification of Anti-angiogenic Biflavonoids from Edible Garcinia Species.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,65(38),8348-8355. |
| MLA | Li, Ping,et al."Using Ultra-Performance Liquid Chromatography Quadrupole Time of Flight Mass Spectrometry-Based Chemometrics for the Identification of Anti-angiogenic Biflavonoids from Edible Garcinia Species".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 65.38(2017):8348-8355. |
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| 10.1021@acs.jafc.7b0（3340KB） | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | 浏览 下载 | |
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