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题名: Qualitative and quantitative analysis of diterpenoids in Salvia species by liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry
作者: Zhou, Yan2; null(许刚)1, 2; Choi, Franky Fung Kei2; Ding, Li-Sheng3; Bin Han, Quan2; Song, Jing Zheng2; Qiao, Chun Feng2; null(赵勤实)1; Xu, Hong-Xi2
刊名: JOURNAL OF CHROMATOGRAPHY A
关键词: Salvia species ; UHPLC-QTOF-MS/MS ; Diterpenoids ; Fragmentation pathway ; Quantification ; Principal component analysis
英文摘要: An on-line ultra-high-performance liquid chromatography (UHPLC) coupled with photodiode-array detection and quadrupole time-of-flight tandem mass spectrometry (QTOF-MS/MS) method has been optimized and established for the qualitative and quantitative analysis of the important diterpenoids in the methanol extracts of 12 Salvia species. Specific marker components were identified for the classification of the Salvia samples by principal component analysis. The accurate mass measurement within 3 ppm error for all the protonated molecules and Subsequent fragment ions offers higher quality structural information for interpretation of fragmentation pathways of various groups of diterpenoids. Thus. a total of 21 diterpenoids from different Salvio species were separated within 10 min, and were unequivocally or tentatively identified via comparisons with authentic standards and literature. This UHPLC-QTOF-MS/MS method was validated to be sensitive, precise and accurate with the limit of detections at 3.0-16 ng/ml. and the overall intra-day and the inter-clay variations less than 3%. The recovery of the method was in the range of 96.2-101.8%, with relative standard deviation (RSD) less than 3.0%. The results demonstrated that the qualitative and quantitative differences in diterpenoids were not only useful for chemotaxonomy in some Salvia species but also for the standardization and differentiation of large numbers of similar samples. (C) 2009 Elsevier B.V. All rights reserved.
出版日期: 2009-06-12
卷号: 1216, 期号:24, 页码:4847-4858
DOI标识: 10.1016/j.chroma.2009.04.017
语种: 英语
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内容类型: 期刊论文
URI标识: http://ir.kib.ac.cn/handle/151853/5144
Appears in Collections:植物化学与西部植物资源持续利用国家重点实验室_期刊论文

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作者单位: 1.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources W China, Kunming 650204, Yunnan Province, Peoples R China
2.Inst Chinese Med, Hong Kong Jockey Club, Shatin, Hong Kong, Peoples R China
3.Chinese Acad Sci, Chengdu Inst Biol, Chengdu 610041, Sichuan Prov, Peoples R China

Recommended Citation:
Zhou, Y; Xu, G; Choi, FFK; Ding, LS; Bin Han, Q; Song, JZ; Qiao, CF; Zhao, QS; Xu, HX.Qualitative and quantitative analysis of diterpenoids in Salvia species by liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry,JOURNAL OF CHROMATOGRAPHY A,2009,1216(24):4847-4858
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