COI1-Regulated Hydroxylation of Jasmonoyl-L-isoleucine Impairs Nicotiana attenuata's Resistance to the Generalist Herbivore Spodoptera litura
Luo, Ji1,4; Wei, Kun2; Wang, Shuanghua1,4; Zhao, Weiye1; Ma, Canrong1,4; Hettenhausen, Christian1; Wu, Jinsong1; Cao, Guoyan1; Sun, Guiling1; Baldwin, Ian T.3; Wu, Jianqiang1; Wang, Lei1
2016-04-13
Source PublicationJOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume64Issue:14Pages:2822-2831
AbstractThe phytohormone jasmonoyl-L-isoleucine (JA-Ile) is well-known as the key signaling molecule that elicits plant defense responses after insect herbivory. Oxidation, which is catalyzed by the cytochrome P450s of the CYP94 family, is thought to be one of the main catabolic pathways of JA-Ile. In this study, we identified four CYP94B3 homologues in the wild tobacco plant Nicotiana attenuata. Individually silencing the four homologues revealed that NaCYP94B3 like-1 and NaCYP94B3 like-2, but not NaCYP94B3 like-3 and NaCYP94B3 like-4, are involved in the C-12-hydroxylation of JA-Ile. Simultaneously silencing three of the NaCYP94B3 like genes, NaCYP94B3 like-1,-2, and -4-, in the VIGS-NaCYP94B3s plants doubled herbivory-induced JA-Ile levels and greatly enhanced plant resistance to the generalist insect herbivore, Spodoptera litura. The poor larval performance was strongly correlated with the high concentrations of several JA-Ile-dependent direct defense metabolites in VIGS-NaCYP94B3s plants. Furthermore, we show that the abundance of 12-hydroxy-JA-Ile was dependent on JA-Ile levels as well as COI1, the receptor of JA-Ile. COI1 appeared to transcriptionally control NaCYP94B3 like-1 and -2 and thus regulates the catabolism of its own ligand molecule, JA-Ile. These results highlight the important role of JA-Ile degradation in jasmonate homeostasis and provide new insight into the feedback regulation of JA-Ile catabolism. Given that silencing these CYP94 genes did not detectably alter plant growth and highly increased plant defense levels, we propose that CYP94B3 genes can be potential targets for genetic improvement of herbivore-resistant crops.
KeywordJa-ile Catabolism Defense Plant-insect Interaction Cytochrome P450 Nicotiana Attenuata Spodoptera Litura
DOI10.1021/acs.jafc.5b06056
Indexed BySCI
Language英语
WOS Research AreaAgriculture ; Chemistry ; Food Science & Technology
WOS SubjectAgriculture, Multidisciplinary ; Chemistry, Applied ; Food Science & Technology
WOS IDWOS:000374275100003
Citation statistics
Cited Times:11[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/26212
Collection植物化学与西部植物资源持续利用国家重点实验室
Affiliation1.Chinese Acad Sci, Kunming Inst Bot, Dept Econ Plants & Biotechnol, Kunming 650201, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Peoples R China
3.Max Planck Inst Chem Ecol, Dept Mol Ecol, D-07745 Jena, Germany
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Luo, Ji,Wei, Kun,Wang, Shuanghua,et al. COI1-Regulated Hydroxylation of Jasmonoyl-L-isoleucine Impairs Nicotiana attenuata's Resistance to the Generalist Herbivore Spodoptera litura[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2016,64(14):2822-2831.
APA Luo, Ji.,Wei, Kun.,Wang, Shuanghua.,Zhao, Weiye.,Ma, Canrong.,...&Wang, Lei.(2016).COI1-Regulated Hydroxylation of Jasmonoyl-L-isoleucine Impairs Nicotiana attenuata's Resistance to the Generalist Herbivore Spodoptera litura.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,64(14),2822-2831.
MLA Luo, Ji,et al."COI1-Regulated Hydroxylation of Jasmonoyl-L-isoleucine Impairs Nicotiana attenuata's Resistance to the Generalist Herbivore Spodoptera litura".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 64.14(2016):2822-2831.
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