Ganoderma lingzhi is a valuable, edible and medicinal fungus that has been widely used for the prevention and treatment of a broad range of diseases. In this study, spirolingzhines A-D, four meroterpenoids with a spiro[benzofuran-2,1'-cyclopentanel motif, lingzhines A-F, six meroterpenoids with diverse ring systems, along with two known compounds were isolated from the fruiting bodies of this fungus. The structures and stereochemistry of these substances were determined by using spectroscopic, X-ray crystallographic and computational methods. Chiral HPLC was used to separate (-)- and (+)-antipodes of seven of ten meroterpenoids, which were isolated from the fungus as racemic mixtures. Several of the metabolites were found to promote proliferation of neural stem cells (NSCs) and, as such, they constitute a class of NSC stimulators. The most potent member of this series, (-)-spirolingzhine A, was shown to affect NSC cell cycle progression using the 5-bromo-2-deoxyuridine (BrdU) incorporation assay. (C) 2015 Elsevier Ltd. All rights reserved.
NSFC-joint Foundation of Yunnan Province [U1202222]
; Open Research Fund of State Key Laboratory Breeding Base of Systematic Research, Development and Utilization of Chinese Medicine Resources, National Natural Science Foundation of China [21472199, 81322030, 31271579]
; "South Drug" Research Collaborative Innovation Organization, Young and Middle Aged Academic Leaders of Kunming, West Light Foundation of the Chinese Academy of Sciences, National Key Basic Research Program of China [2013CB910900]
1.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Peoples R China 2.Chengdu Univ Tradit Chinese Med, Coll Pharm, Chengdu 610075, Peoples R China 3.Henan Univ Tradit Chinese Med, Coll Pharm, Zhengzhou 450008, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Chinese Acad Sci & Yunnan Prov, Kunming Inst Zool, Key Lab Anim Models & Human Dis Mech, Kunming 650223, Peoples R China