Structural Analysis and Anticoagulant Activities of the Novel Sulfated Fucan Possessing a Regular Well-Defined Repeating Unit from Sea Cucumber

doi:10.3390/md13042063

	Structural Analysis and Anticoagulant Activities of the Novel Sulfated Fucan Possessing a Regular Well-Defined Repeating Unit from Sea Cucumber
	Wu, Mingyi 1; Xu, Li 1,2; Zhao, Longyan1,2 ; Xiao, Chuang1,2 ; Gao, Na 1,2; Luo, Lan 1; Yang, Lian 1; Li, Zi 1; Chen, Lingyun 3; Zhao, Jinhua1
	2015
发表期刊	MARINE DRUGS
卷号	13 期号:4 页码:2063-2084
摘要	Sulfated fucans, the complex polysaccharides, exhibit various biological activities. Herein, we purified two fucans from the sea cucumbers Holothuria edulis and Ludwigothurea grisea. Their structures were verified by means of HPGPC, FT-IR, GC-MS and NMR. As a result, a novel structural motif for this type of polymers is reported. The fucans have a unique structure composed of a central core of regular (1 -> 2) and (1 -> 3)-linked tetrasaccharide repeating units. Approximately 50% of the units from L. grisea (100% for H. edulis fucan) contain sides of oligosaccharides formed by nonsulfated fucose units linked to the O-4 position of the central core. Anticoagulant activity assays indicate that the sea cucumber fucans strongly inhibit human blood clotting through the intrinsic pathways of the coagulation cascade. Moreover, the mechanism of anticoagulant action of the fucans is selective inhibition of thrombin activity by heparin cofactor II. The distinctive tetrasaccharide repeating units contribute to the anticoagulant action. Additionally, unlike the fucans from marine alga, although the sea cucumber fucans have great molecular weights and affluent sulfates, they do not induce platelet aggregation. Overall, our results may be helpful in understanding the structure-function relationships of the well-defined polysaccharides from invertebrate as new types of safer anticoagulants.
DOI	10.3390/md13042063
收录类别	SCI
语种	英语
WOS记录号	WOS:000353715900024
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/20683
专题	植物化学与西部植物资源持续利用国家重点实验室
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Peoples R China 2.Univ Chinese Acad Sci, Sch Life Sci, Beijing 100000, Peoples R China 3.Yunnan Univ TCM, Dept Pharm, Kunming 650200, Peoples R China
推荐引用方式 GB/T 7714	Wu, Mingyi,Xu, Li,Zhao, Longyan,et al. Structural Analysis and Anticoagulant Activities of the Novel Sulfated Fucan Possessing a Regular Well-Defined Repeating Unit from Sea Cucumber[J]. MARINE DRUGS,2015,13(4):2063-2084.
APA	Wu, Mingyi.,Xu, Li.,Zhao, Longyan.,Xiao, Chuang.,Gao, Na.,...&Zhao, Jinhua.(2015).Structural Analysis and Anticoagulant Activities of the Novel Sulfated Fucan Possessing a Regular Well-Defined Repeating Unit from Sea Cucumber.MARINE DRUGS,13(4),2063-2084.
MLA	Wu, Mingyi,et al."Structural Analysis and Anticoagulant Activities of the Novel Sulfated Fucan Possessing a Regular Well-Defined Repeating Unit from Sea Cucumber".MARINE DRUGS 13.4(2015):2063-2084.

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