Structural Analysis and Anticoagulant Activities of the Novel Sulfated Fucan Possessing a Regular Well-Defined Repeating Unit from Sea Cucumber
Wu, Mingyi1; Xu, Li1,2; Zhao, Longyan1,2; Xiao, Chuang1,2; Gao, Na1,2; Luo, Lan1; Yang, Lian1; Li, Zi1; Chen, Lingyun3; Zhao, Jinhua1
2015
发表期刊MARINE DRUGS
卷号13期号:4页码:2063-2084
摘要Sulfated fucans, the complex polysaccharides, exhibit various biological activities. Herein, we purified two fucans from the sea cucumbers Holothuria edulis and Ludwigothurea grisea. Their structures were verified by means of HPGPC, FT-IR, GC-MS and NMR. As a result, a novel structural motif for this type of polymers is reported. The fucans have a unique structure composed of a central core of regular (1 -> 2) and (1 -> 3)-linked tetrasaccharide repeating units. Approximately 50% of the units from L. grisea (100% for H. edulis fucan) contain sides of oligosaccharides formed by nonsulfated fucose units linked to the O-4 position of the central core. Anticoagulant activity assays indicate that the sea cucumber fucans strongly inhibit human blood clotting through the intrinsic pathways of the coagulation cascade. Moreover, the mechanism of anticoagulant action of the fucans is selective inhibition of thrombin activity by heparin cofactor II. The distinctive tetrasaccharide repeating units contribute to the anticoagulant action. Additionally, unlike the fucans from marine alga, although the sea cucumber fucans have great molecular weights and affluent sulfates, they do not induce platelet aggregation. Overall, our results may be helpful in understanding the structure-function relationships of the well-defined polysaccharides from invertebrate as new types of safer anticoagulants.
DOI10.3390/md13042063
收录类别SCI
语种英语
WOS研究方向Pharmacology & Pharmacy
WOS类目Chemistry, Medicinal
WOS记录号WOS:000353715900024
引用统计
被引频次:20[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/20683
专题植物化学与西部植物资源持续利用国家重点实验室
作者单位1.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Peoples R China
2.Univ Chinese Acad Sci, Sch Life Sci, Beijing 100000, Peoples R China
3.Yunnan Univ TCM, Dept Pharm, Kunming 650200, Peoples R China
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Wu, Mingyi,Xu, Li,Zhao, Longyan,et al. Structural Analysis and Anticoagulant Activities of the Novel Sulfated Fucan Possessing a Regular Well-Defined Repeating Unit from Sea Cucumber[J]. MARINE DRUGS,2015,13(4):2063-2084.
APA Wu, Mingyi.,Xu, Li.,Zhao, Longyan.,Xiao, Chuang.,Gao, Na.,...&Zhao, Jinhua.(2015).Structural Analysis and Anticoagulant Activities of the Novel Sulfated Fucan Possessing a Regular Well-Defined Repeating Unit from Sea Cucumber.MARINE DRUGS,13(4),2063-2084.
MLA Wu, Mingyi,et al."Structural Analysis and Anticoagulant Activities of the Novel Sulfated Fucan Possessing a Regular Well-Defined Repeating Unit from Sea Cucumber".MARINE DRUGS 13.4(2015):2063-2084.
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