Wang,L (reprint author),Kunming Med Univ,Sch Pharmaceut Sci,Kunming 650500,Peoples R China.
We studied the alkaloid constituents and bioactivity of Sophora flavescens (Leguminosae). The constituents were isolated with silica gel column chromatography, semi-preparative HPLC, Sephadex LH-20, and MPLC packed with MCI gel, and their structures were elucidated on the basis of physical characteristics and spectral data. Compounds 1 and 5-8 were evaluated for their in vitro cytotoxicity against human tumor HL-60, SMMC-7721, A-549, MCF-7, and SW-480 cell lines. Ten alkaloids were obtained, and their structures were identified as 17 beta-hydroxysophoridine (1), matrine (2), sophocarpine (3), sophoridine (4), isomatrine (5), 7,11-dehydromatrine (6), mamanine (7), sophoranol (8), oxymatrine (9), and oxysophocarpine (10). Compound 1 is a new alkaloid, and compound 7 was isolated from the Sophora flavescens for the first time. None of the compounds were cytotoxic to five human cancer cell lines.
National Natural Science Foundation of China [21272241, 21172223, 31271579, 81322030]
; Open Research Fund of State Key Laboratory Breeding Base of Systematic Research, Development and Utilization of Chinese Medicine Resources
; Middle Aged Academic Leaders of Kunming, a project from Center of Cooperative Innovation for South China Medicine of Yunnan Province
; National Key Basic Research Program of China [2013CB910900]
1.Kunming Med Univ, Sch Pharmaceut Sci, Kunming 650500, Peoples R China 2.Kunming Med Univ, Yunnan Key Lab Pharmacol Nat Prod, Kunming 650500, Peoples R China 3.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Peoples R China
Wang Lei;Wu Xing-De;He Juan;Li Gen-Tao;Peng Li-Yan;Li Yan;Song Liu-Dong;Zhao Qin-Shi.A New Quinolizidine Alkaloid from Sophora flavescens,CHEMISTRY OF NATURAL COMPOUNDS,2014,70(46):8852-8857