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Scopoletin is a phytoalexin against Alternaria alternata in wild tobacco dependent on jasmonate signalling
Sun, Huanhuan1; Wang, Lei1; Zhang, Baoqin2; Ma, Junhong3; Hettenhausen, Christian1; Cao, Guoyan1; Sun, Guiling1; Wu, Jianqiang1; Wu, Jinsong1; Wu, JS (reprint author), Chinese Acad Sci, Kunming Inst Bot, Key Lab Econ Plants & Biotechnol, Lanhei Rd 132, Kunming 650201, Peoples R China.; jinsongwu@mail.kib.ac.cn
2014-08-01
Source PublicationJOURNAL OF EXPERIMENTAL BOTANY
ISSN0022-0957
Volume65Issue:15Pages:4305-4315
Contribution Rank第一
AbstractAlternaria alternata (tobacco pathotype) is a necrotrophic fungus causing severe losses in Nicotiana species by infection of mature leaves. Similar to what has been observed in cultivated tobacco, N. tabacum, young leaves of wild tobacco, N. attenuata, were more resistant to A. alternata than mature leaves, and this was correlated with stronger blue fluorescence induced after infection. However, the nature of the fluorescence-emitting compound, its role in defence, and its regulation were not clear. Silencing feruloyl-CoA 6'-hydroxylase 1 (F6'H1), the gene encoding the key enzyme for scopoletin biosynthesis, by virus-induced gene silencing (VIGS) revealed that the blue fluorescence was mainly emitted by scopoletin and its beta-glycoside form, scopolin. Further analysis showed that scopoletin exhibited strong antifungal activity against A. alternata in vitro and in vivo. Importantly, jasmonic acid (JA) levels were highly elicited in young leaves but much less in mature leaves after infection; and fungus-elicited scopoletin was absent in JA-deficient plants, but was largely restored with methyl jasmonate treatments. Consistent with this, plants strongly impaired in JA biosynthesis and perception were highly susceptible to A. alternata in the same way scopoletin/scopolin-depleted VIGS F6'H1 plants. Furthermore, silencing MYC2, a master regulator of most JA responses, reduced A. alternata-induced NaF6'H1 transcripts and scopoletin. Thus, it is concluded that JA signalling is activated in N. attenuata leaves after infection, which subsequently regulates scopoletin biosynthesis for the defence against A. alternata partly through MYC2, and higher levels of scopoletin accumulated in young leaves account for their strong resistance.
KeywordAlternaria Alternata Feruloyl-coa 6 '-hydroxylase 1 (F6 ' H1) Jasmonic Acid (Ja) Myc2 Nicotiana Attenuata Scopoletin Virus-induced Gene Silencing (Vigs)
Subject AreaPlant Sciences
DOI10.1093/jxb/eru203
Indexed BySCI
Language英语
WOS Research AreaPlant Sciences
WOS SubjectPlant Sciences
WOS IDWOS:000339954300016
Citation statistics
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/18307
Collection资源植物与生物技术所级重点实验室
Corresponding AuthorWu, JS (reprint author), Chinese Acad Sci, Kunming Inst Bot, Key Lab Econ Plants & Biotechnol, Lanhei Rd 132, Kunming 650201, Peoples R China.; jinsongwu@mail.kib.ac.cn
Affiliation1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Econ Plants & Biotechnol, Kunming 650201, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
3.Yunnan Acad Tobacco Agr Sci, Kunming 650031, Peoples R China
Recommended Citation
GB/T 7714
Sun, Huanhuan,Wang, Lei,Zhang, Baoqin,et al. Scopoletin is a phytoalexin against Alternaria alternata in wild tobacco dependent on jasmonate signalling[J]. JOURNAL OF EXPERIMENTAL BOTANY,2014,65(15):4305-4315.
APA Sun, Huanhuan.,Wang, Lei.,Zhang, Baoqin.,Ma, Junhong.,Hettenhausen, Christian.,...&jinsongwu@mail.kib.ac.cn.(2014).Scopoletin is a phytoalexin against Alternaria alternata in wild tobacco dependent on jasmonate signalling.JOURNAL OF EXPERIMENTAL BOTANY,65(15),4305-4315.
MLA Sun, Huanhuan,et al."Scopoletin is a phytoalexin against Alternaria alternata in wild tobacco dependent on jasmonate signalling".JOURNAL OF EXPERIMENTAL BOTANY 65.15(2014):4305-4315.
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