Scopoletin is a phytoalexin against Alternaria alternata in wild tobacco dependent on jasmonate signalling
Sun, Huanhuan1; Wang, Lei1; Zhang, Baoqin2; Ma, Junhong3; Hettenhausen, Christian1; Cao, Guoyan1; Sun, Guiling1; Wu, Jianqiang1; Wu, Jinsong1; Wu, JS (reprint author), Chinese Acad Sci, Kunming Inst Bot, Key Lab Econ Plants & Biotechnol, Lanhei Rd 132, Kunming 650201, Peoples R China.; jinsongwu@mail.kib.ac.cn
2014-08-01
发表期刊JOURNAL OF EXPERIMENTAL BOTANY
ISSN0022-0957
卷号65期号:15页码:4305-4315
产权排序第一
摘要Alternaria alternata (tobacco pathotype) is a necrotrophic fungus causing severe losses in Nicotiana species by infection of mature leaves. Similar to what has been observed in cultivated tobacco, N. tabacum, young leaves of wild tobacco, N. attenuata, were more resistant to A. alternata than mature leaves, and this was correlated with stronger blue fluorescence induced after infection. However, the nature of the fluorescence-emitting compound, its role in defence, and its regulation were not clear. Silencing feruloyl-CoA 6'-hydroxylase 1 (F6'H1), the gene encoding the key enzyme for scopoletin biosynthesis, by virus-induced gene silencing (VIGS) revealed that the blue fluorescence was mainly emitted by scopoletin and its beta-glycoside form, scopolin. Further analysis showed that scopoletin exhibited strong antifungal activity against A. alternata in vitro and in vivo. Importantly, jasmonic acid (JA) levels were highly elicited in young leaves but much less in mature leaves after infection; and fungus-elicited scopoletin was absent in JA-deficient plants, but was largely restored with methyl jasmonate treatments. Consistent with this, plants strongly impaired in JA biosynthesis and perception were highly susceptible to A. alternata in the same way scopoletin/scopolin-depleted VIGS F6'H1 plants. Furthermore, silencing MYC2, a master regulator of most JA responses, reduced A. alternata-induced NaF6'H1 transcripts and scopoletin. Thus, it is concluded that JA signalling is activated in N. attenuata leaves after infection, which subsequently regulates scopoletin biosynthesis for the defence against A. alternata partly through MYC2, and higher levels of scopoletin accumulated in young leaves account for their strong resistance.
关键词Alternaria Alternata Feruloyl-coa 6 '-hydroxylase 1 (F6 ' H1) Jasmonic Acid (Ja) Myc2 Nicotiana Attenuata Scopoletin Virus-induced Gene Silencing (Vigs)
资助信息Chinese Academy of Sciences; Key Project of Applied Basic Research Program of Yunnan; Scientific Research Foundation of the Ministry of Education of China for Returned Scholars
学科领域Plant Sciences
DOI10.1093/jxb/eru203
收录类别SCI
语种英语
WOS研究方向Plant Sciences
WOS类目Plant Sciences
WOS记录号WOS:000339954300016
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文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/18307
专题资源植物与生物技术所级重点实验室
通讯作者Wu, JS (reprint author), Chinese Acad Sci, Kunming Inst Bot, Key Lab Econ Plants & Biotechnol, Lanhei Rd 132, Kunming 650201, Peoples R China.; jinsongwu@mail.kib.ac.cn
作者单位1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Econ Plants & Biotechnol, Kunming 650201, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
3.Yunnan Acad Tobacco Agr Sci, Kunming 650031, Peoples R China
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Sun, Huanhuan,Wang, Lei,Zhang, Baoqin,et al. Scopoletin is a phytoalexin against Alternaria alternata in wild tobacco dependent on jasmonate signalling[J]. JOURNAL OF EXPERIMENTAL BOTANY,2014,65(15):4305-4315.
APA Sun, Huanhuan.,Wang, Lei.,Zhang, Baoqin.,Ma, Junhong.,Hettenhausen, Christian.,...&jinsongwu@mail.kib.ac.cn.(2014).Scopoletin is a phytoalexin against Alternaria alternata in wild tobacco dependent on jasmonate signalling.JOURNAL OF EXPERIMENTAL BOTANY,65(15),4305-4315.
MLA Sun, Huanhuan,et al."Scopoletin is a phytoalexin against Alternaria alternata in wild tobacco dependent on jasmonate signalling".JOURNAL OF EXPERIMENTAL BOTANY 65.15(2014):4305-4315.
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