| Structural characterization and immunomodulatory effect of a polysaccharide HCP-2 from Houttuynia cordata | |
Cheng, Bao-Hui1,2; Chan, Judy Yuet-Wa1,2; Chan, Ben Chung-Lap1,2; Lin, Huang-Quan1,2; Han, Xiao-Qiang1,2; Zhou, Xuelin1,2; Wan, David Chi-Cheong3; Wang, Yi-Fen4 ; Leung, Ping-Chung1,2; Fung, Kwok-Pui1,2,3; Bik-San Lau, Clara1,2
| |
| 通讯作者 | Bik-San Lau, C (reprint author), Chinese Univ Hong Kong, Inst Chinese Med, E305,Sci Ctr East Block, Shatin, Hong Kong, Peoples R China. ; claralau@cuhk.edu.hk |
| 2014-03-15 | |
| 发表期刊 | CARBOHYDRATE POLYMERS
 |
| ISSN | 0144-8617 |
| 卷号 | 103页码:244-249 |
| 摘要 | Immunomodulation of natural polysaccharides has been the hot topic of research in recent years. In order to explore the immunomodulatory effect of Houttuynia cordata Thunb., the water extract was studied and a polysaccharide HCP-2 with molecular weight of 60,000 Da was isolated by chromatography using DEAE Sepharose CL-6B and Sephacryl S-400 HR columns. The structure characterization of HCP-2 was performed by Fourier transform infrared spectroscopy (FTIR), acidic hydrolysis, PMP derivation, HPLC analysis and nuclear magnetic resonance spectra (NMR). HCP-2 was elucidated as a pectic polysaccharide with a linear chain of 1,4-linked alpha-D-galacturonic acid residues in which part of the 6-carboxyl groups were methyl esterified and part of 2-hydroxyl groups were acetylated. The bioactivity assays showed that HCP-2 could increase the secretions of interleukin-1 beta (IL-1 beta), tumor necrosis factor-alpha (TNF-alpha), macrophage inhibitory protein-1 alpha (MIP-1 alpha), macrophage inhibitory protein-1 beta (MIP-1 beta), and RANTES (regulated on activation, normal T cell expressed and secreted) in human peripheral blood mononuclear cells (PBMCs), which play critical roles in the innate immune system and shape the adaptive immunity. Our results implied that HCP-2 could be an immune enhancer. (C) 2013 Elsevier Ltd. All rights reserved. |
| 关键词 | Houttuynia Cordata Pectic Polysaccharide Hcp-2 Structural Characterization Immunomodulation |
| 学科领域 | Chemistry, Applied ; Chemistry, Organic ; Polymer Science |
| DOI | 10.1016/j.carbpol.2013.12.048 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000332812600033 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.kib.ac.cn/handle/151853/18049 |
| 专题 | 植物化学与西部植物资源持续利用国家重点实验室 |
| 作者单位 | 1.Chinese Univ Hong Kong, Inst Chinese Med, Shatin, Hong Kong, Peoples R China 2.Chinese Univ Hong Kong, State Key Lab Phytochem & Plant Resources West Ch, Shatin, Hong Kong, Peoples R China 3.Chinese Univ Hong Kong, Sch Biomed Sci, Fac Med, Shatin, Hong Kong, Peoples R China 4.Chinese Acad Sci, State Key Lab Phytochem & Plant Resources West Ch, Kunming Inst Bot, Kunming 650201, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cheng, Bao-Hui,Chan, Judy Yuet-Wa,Chan, Ben Chung-Lap,et al. Structural characterization and immunomodulatory effect of a polysaccharide HCP-2 from Houttuynia cordata[J]. CARBOHYDRATE POLYMERS,2014,103:244-249. |
| APA | Cheng, Bao-Hui.,Chan, Judy Yuet-Wa.,Chan, Ben Chung-Lap.,Lin, Huang-Quan.,Han, Xiao-Qiang.,...&Bik-San Lau, Clara.(2014).Structural characterization and immunomodulatory effect of a polysaccharide HCP-2 from Houttuynia cordata.CARBOHYDRATE POLYMERS,103,244-249. |
| MLA | Cheng, Bao-Hui,et al."Structural characterization and immunomodulatory effect of a polysaccharide HCP-2 from Houttuynia cordata".CARBOHYDRATE POLYMERS 103(2014):244-249. |
| 条目包含的文件 | 下载所有文件 | |||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Cheng-2014-Structura（736KB） | 开放获取 | CC BY-NC-SA | 浏览 下载 | |||
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
修改评论