Structural characterization and immunomodulatory effect of a polysaccharide HCP-2 from Houttuynia cordata
Cheng, Bao-Hui1,2; Chan, Judy Yuet-Wa1,2; Chan, Ben Chung-Lap1,2; Lin, Huang-Quan1,2; Han, Xiao-Qiang1,2; Zhou, Xuelin1,2; Wan, David Chi-Cheong3; Wang, Yi-Fen4; Leung, Ping-Chung1,2; Fung, Kwok-Pui1,2,3; Bik-San Lau, Clara1,2
Corresponding AuthorBik-San Lau, C (reprint author), Chinese Univ Hong Kong, Inst Chinese Med, E305,Sci Ctr East Block, Shatin, Hong Kong, Peoples R China. ; claralau@cuhk.edu.hk
2014-03-15
Source PublicationCARBOHYDRATE POLYMERS
ISSN0144-8617
Volume103Pages:244-249
AbstractImmunomodulation of natural polysaccharides has been the hot topic of research in recent years. In order to explore the immunomodulatory effect of Houttuynia cordata Thunb., the water extract was studied and a polysaccharide HCP-2 with molecular weight of 60,000 Da was isolated by chromatography using DEAE Sepharose CL-6B and Sephacryl S-400 HR columns. The structure characterization of HCP-2 was performed by Fourier transform infrared spectroscopy (FTIR), acidic hydrolysis, PMP derivation, HPLC analysis and nuclear magnetic resonance spectra (NMR). HCP-2 was elucidated as a pectic polysaccharide with a linear chain of 1,4-linked alpha-D-galacturonic acid residues in which part of the 6-carboxyl groups were methyl esterified and part of 2-hydroxyl groups were acetylated. The bioactivity assays showed that HCP-2 could increase the secretions of interleukin-1 beta (IL-1 beta), tumor necrosis factor-alpha (TNF-alpha), macrophage inhibitory protein-1 alpha (MIP-1 alpha), macrophage inhibitory protein-1 beta (MIP-1 beta), and RANTES (regulated on activation, normal T cell expressed and secreted) in human peripheral blood mononuclear cells (PBMCs), which play critical roles in the innate immune system and shape the adaptive immunity. Our results implied that HCP-2 could be an immune enhancer. (C) 2013 Elsevier Ltd. All rights reserved.
KeywordHouttuynia Cordata Pectic Polysaccharide Hcp-2 Structural Characterization Immunomodulation
Subject AreaChemistry, Applied ; Chemistry, Organic ; Polymer Science
DOI10.1016/j.carbpol.2013.12.048
Indexed BySCI
Language英语
WOS Research AreaChemistry ; Polymer Science
WOS SubjectChemistry, Applied ; Chemistry, Organic ; Polymer Science
WOS IDWOS:000332812600033
Citation statistics
Cited Times:19[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/18049
Collection植物化学与西部植物资源持续利用国家重点实验室
Affiliation1.Chinese Univ Hong Kong, Inst Chinese Med, Shatin, Hong Kong, Peoples R China
2.Chinese Univ Hong Kong, State Key Lab Phytochem & Plant Resources West Ch, Shatin, Hong Kong, Peoples R China
3.Chinese Univ Hong Kong, Sch Biomed Sci, Fac Med, Shatin, Hong Kong, Peoples R China
4.Chinese Acad Sci, State Key Lab Phytochem & Plant Resources West Ch, Kunming Inst Bot, Kunming 650201, Peoples R China
Recommended Citation
GB/T 7714
Cheng, Bao-Hui,Chan, Judy Yuet-Wa,Chan, Ben Chung-Lap,et al. Structural characterization and immunomodulatory effect of a polysaccharide HCP-2 from Houttuynia cordata[J]. CARBOHYDRATE POLYMERS,2014,103:244-249.
APA Cheng, Bao-Hui.,Chan, Judy Yuet-Wa.,Chan, Ben Chung-Lap.,Lin, Huang-Quan.,Han, Xiao-Qiang.,...&Bik-San Lau, Clara.(2014).Structural characterization and immunomodulatory effect of a polysaccharide HCP-2 from Houttuynia cordata.CARBOHYDRATE POLYMERS,103,244-249.
MLA Cheng, Bao-Hui,et al."Structural characterization and immunomodulatory effect of a polysaccharide HCP-2 from Houttuynia cordata".CARBOHYDRATE POLYMERS 103(2014):244-249.
Files in This Item: Download All
File Name/Size DocType Version Access License
Cheng-2014-Structura(736KB) 开放获取CC BY-NC-SAView Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Cheng, Bao-Hui]'s Articles
[Chan, Judy Yuet-Wa]'s Articles
[Chan, Ben Chung-Lap]'s Articles
Baidu academic
Similar articles in Baidu academic
[Cheng, Bao-Hui]'s Articles
[Chan, Judy Yuet-Wa]'s Articles
[Chan, Ben Chung-Lap]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Cheng, Bao-Hui]'s Articles
[Chan, Judy Yuet-Wa]'s Articles
[Chan, Ben Chung-Lap]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Cheng-2014-Structural character.pdf
Format: Adobe PDF
This file does not support browsing at this time
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.