Structural characterization and immunomodulatory effect of a polysaccharide HCP-2 from Houttuynia cordata
Cheng, Bao-Hui1,2; Chan, Judy Yuet-Wa1,2; Chan, Ben Chung-Lap1,2; Lin, Huang-Quan1,2; Han, Xiao-Qiang1,2; Zhou, Xuelin1,2; Wan, David Chi-Cheong3; Wang, Yi-Fen4; Leung, Ping-Chung1,2; Fung, Kwok-Pui1,2,3; Bik-San Lau, Clara1,2
2014-03-15
发表期刊CARBOHYDRATE POLYMERS
ISSN0144-8617
卷号103页码:244-249
摘要Immunomodulation of natural polysaccharides has been the hot topic of research in recent years. In order to explore the immunomodulatory effect of Houttuynia cordata Thunb., the water extract was studied and a polysaccharide HCP-2 with molecular weight of 60,000 Da was isolated by chromatography using DEAE Sepharose CL-6B and Sephacryl S-400 HR columns. The structure characterization of HCP-2 was performed by Fourier transform infrared spectroscopy (FTIR), acidic hydrolysis, PMP derivation, HPLC analysis and nuclear magnetic resonance spectra (NMR). HCP-2 was elucidated as a pectic polysaccharide with a linear chain of 1,4-linked alpha-D-galacturonic acid residues in which part of the 6-carboxyl groups were methyl esterified and part of 2-hydroxyl groups were acetylated. The bioactivity assays showed that HCP-2 could increase the secretions of interleukin-1 beta (IL-1 beta), tumor necrosis factor-alpha (TNF-alpha), macrophage inhibitory protein-1 alpha (MIP-1 alpha), macrophage inhibitory protein-1 beta (MIP-1 beta), and RANTES (regulated on activation, normal T cell expressed and secreted) in human peripheral blood mononuclear cells (PBMCs), which play critical roles in the innate immune system and shape the adaptive immunity. Our results implied that HCP-2 could be an immune enhancer. (C) 2013 Elsevier Ltd. All rights reserved.
关键词Houttuynia Cordata Pectic Polysaccharide Hcp-2 Structural Characterization Immunomodulation
学科领域Chemistry, Applied ; Chemistry, Organic ; Polymer Science
DOI10.1016/j.carbpol.2013.12.048
收录类别SCI
语种英语
WOS研究方向Chemistry ; Polymer Science
WOS类目Chemistry, Applied ; Chemistry, Organic ; Polymer Science
WOS记录号WOS:000332812600033
引用统计
被引频次:17[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/18049
专题植物化学与西部植物资源持续利用国家重点实验室
作者单位1.Chinese Univ Hong Kong, Inst Chinese Med, Shatin, Hong Kong, Peoples R China
2.Chinese Univ Hong Kong, State Key Lab Phytochem & Plant Resources West Ch, Shatin, Hong Kong, Peoples R China
3.Chinese Univ Hong Kong, Sch Biomed Sci, Fac Med, Shatin, Hong Kong, Peoples R China
4.Chinese Acad Sci, State Key Lab Phytochem & Plant Resources West Ch, Kunming Inst Bot, Kunming 650201, Peoples R China
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Cheng, Bao-Hui,Chan, Judy Yuet-Wa,Chan, Ben Chung-Lap,et al. Structural characterization and immunomodulatory effect of a polysaccharide HCP-2 from Houttuynia cordata[J]. CARBOHYDRATE POLYMERS,2014,103:244-249.
APA Cheng, Bao-Hui.,Chan, Judy Yuet-Wa.,Chan, Ben Chung-Lap.,Lin, Huang-Quan.,Han, Xiao-Qiang.,...&Bik-San Lau, Clara.(2014).Structural characterization and immunomodulatory effect of a polysaccharide HCP-2 from Houttuynia cordata.CARBOHYDRATE POLYMERS,103,244-249.
MLA Cheng, Bao-Hui,et al."Structural characterization and immunomodulatory effect of a polysaccharide HCP-2 from Houttuynia cordata".CARBOHYDRATE POLYMERS 103(2014):244-249.
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