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题名: Structure and anticoagulant activity of fucosylated glycosaminoglycan degraded by deaminative cleavage
作者: Zhao, Longyan1, 2; Lai, Sensen1, 3, 4; Huang, Rong5; Wu, Mingyi1; Gao, Na1; Xu, Li1, 2; null(秦红波)1; Peng, Wenlie3; null(赵金华)1
刊名: CARBOHYDRATE POLYMERS
关键词: Sea cucumber ; Glycosaminoglycan ; Deacetylation ; Deaminative cleavage ; Anticoagulant activity
英文摘要: Fucosylated glycosaminoglycans (FGs) are complex glycosaminoglycans that exhibit potent anticoagulant activity. To study the relationship between molecular size and biological activity, oligosaccharides with (2,5)-anhydro-D-talose units at new reducing ends were prepared by hydrazine deacetylation and nitrous acid depolymerization. The product chemical structures were analyzed by one- and two-dimensional NMR methods. Additionally, anticoagulant activities were evaluated by clotting assay and chromogenic substrate cleavage. The results demonstrated that under mild deacetylation and deaminative cleavage conditions, both products were relatively homogeneous and sulfated fucose branch types and sulfate sub-stituents remained stable. These depolymerized FGs with different molecular sizes had potent intrinsic anticoagulant activities, which were similar to those that were obtained by free-radical depolymerization with similar molecular weights. Decreasing molecular weight may weaken activity but not significantly affect factor Xase and heparin cofactor II (HCII)-mediated thrombin inhibition. (C) 2013 Elsevier Ltd. All rights reserved.
出版日期: 2013-11-06
卷号: 98, 期号:2, 页码:1514-1523
语种: 英语
ISSN号: 0144-8617
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内容类型: 期刊论文
URI标识: http://ir.kib.ac.cn/handle/151853/17420
Appears in Collections:植物化学与西部植物资源持续利用国家重点实验室_期刊论文

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作者单位: 1.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Sun Yat Sen Univ, Coll Life Sci, Guangzhou 510275, Guangdong, Peoples R China
4.Ganzhou Prov Inst Food & Drug Control, Ganzhou, Peoples R China
5.Yunan Univ, Sch Chem Sci & Technol, Minist Educ, Key Lab Med Chem Nat Resource, Kunming, Peoples R China

Recommended Citation:
Zhao,Longyan;Lai,Sensen;Huang,Rong;Wu,Mingyi;Gao,Na;Xu,Li;Qin,Hongbo;Peng,Wenlie;Zhao,Jinhua.Structure and anticoagulant activity of fucosylated glycosaminoglycan degraded by deaminative cleavage,CARBOHYDRATE POLYMERS,2013,98(2):1514-1523
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