Structure and anticoagulant activity of fucosylated glycosaminoglycan degraded by deaminative cleavage
Zhao, Longyan1,2; Lai, Sensen1,3,4; Huang, Rong5; Wu, Mingyi1; Gao, Na1; Xu, Li1,2; Qin, Hongbo1; Peng, Wenlie3; Zhao, Jinhua1
2013-11-06
发表期刊CARBOHYDRATE POLYMERS
ISSN0144-8617
卷号98期号:2页码:1514-1523
摘要Fucosylated glycosaminoglycans (FGs) are complex glycosaminoglycans that exhibit potent anticoagulant activity. To study the relationship between molecular size and biological activity, oligosaccharides with (2,5)-anhydro-D-talose units at new reducing ends were prepared by hydrazine deacetylation and nitrous acid depolymerization. The product chemical structures were analyzed by one- and two-dimensional NMR methods. Additionally, anticoagulant activities were evaluated by clotting assay and chromogenic substrate cleavage. The results demonstrated that under mild deacetylation and deaminative cleavage conditions, both products were relatively homogeneous and sulfated fucose branch types and sulfate sub-stituents remained stable. These depolymerized FGs with different molecular sizes had potent intrinsic anticoagulant activities, which were similar to those that were obtained by free-radical depolymerization with similar molecular weights. Decreasing molecular weight may weaken activity but not significantly affect factor Xase and heparin cofactor II (HCII)-mediated thrombin inhibition. (C) 2013 Elsevier Ltd. All rights reserved.
关键词Sea Cucumber Glycosaminoglycan Deacetylation Deaminative Cleavage Anticoagulant Activity
资助信息Yunnan Provincial Science and Technology Department in China [2010CI116, 2012BC011, 2012FB177];National Natural Science Foundation of China [81102372];Outstanding Technical Talent Foundation of the Chinese Academy of Sciences;West Light Foundation of the Chinese Academy of Sciences
收录类别SCI
语种英语
引用统计
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/17420
专题植物化学与西部植物资源持续利用国家重点实验室
作者单位1.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Sun Yat Sen Univ, Coll Life Sci, Guangzhou 510275, Guangdong, Peoples R China
4.Ganzhou Prov Inst Food & Drug Control, Ganzhou, Peoples R China
5.Yunan Univ, Sch Chem Sci & Technol, Minist Educ, Key Lab Med Chem Nat Resource, Kunming, Peoples R China
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Zhao, Longyan,Lai, Sensen,Huang, Rong,et al. Structure and anticoagulant activity of fucosylated glycosaminoglycan degraded by deaminative cleavage[J]. CARBOHYDRATE POLYMERS,2013,98(2):1514-1523.
APA Zhao, Longyan.,Lai, Sensen.,Huang, Rong.,Wu, Mingyi.,Gao, Na.,...&Zhao, Jinhua.(2013).Structure and anticoagulant activity of fucosylated glycosaminoglycan degraded by deaminative cleavage.CARBOHYDRATE POLYMERS,98(2),1514-1523.
MLA Zhao, Longyan,et al."Structure and anticoagulant activity of fucosylated glycosaminoglycan degraded by deaminative cleavage".CARBOHYDRATE POLYMERS 98.2(2013):1514-1523.
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