Microfractionation bioactivity-based ultra performance liquid chromatography/quadrupole time-of-flight mass spectrometry for the identification of nuclear factor-kappa B inhibitors and beta(2) adrenergic receptor agonists in an alkaloidal extract of the folk herb Alstonia scholaris

doi:10.1016/j.jchromb.2012.10.004

	Microfractionation bioactivity-based ultra performance liquid chromatography/quadrupole time-of-flight mass spectrometry for the identification of nuclear factor-kappa B inhibitors and beta(2) adrenergic receptor agonists in an alkaloidal extract of the folk herb Alstonia scholaris
	Hou, Yuanyuan 1,2; Cao, Xuelin 3; Wang, Liqiang 1,2; Cheng, Binfeng 1,2; Dong, Linyi 3; Luo, Xiaodong4 ; Bai, Gang 1,2; Gao, Wenyuan 3
通讯作者	Bai, G (reprint author), Nankai Univ, Coll Pharm, State Key Lab Med Chem Biol, Tianjin 300071, Peoples R China.,gangbai@nankai.edu.cn ; pharmgao@tju.edu.cn
	2012-11-01
发表期刊	JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
ISSN	1570-0232
卷号	908 页码:98-104
摘要	Traditional Chinese medicines (TCMs) are generally considered complementary or alternative remedies in most Western countries. The constituents of TCMs are hard to define, and their efficacy is difficult to appraise. Thus, the development of suitable methods for evaluating the relationship between bioactivity and the chemical makeup of complex TCM mixtures remains a great challenge. In the present work, the bioactivity-integrated fingerprints of alkaloidal leaf extracts of Alstonia scholaris, a folk medicinal herb for chronic respiratory diseases, were established by ultra performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC-Q/TOF). This method was coupled with two dual-luciferase reporter assay systems to show nuclear factor-kappa B (NF-kappa B) inhibition and beta(2) adrenergic receptor (beta(2)AR) activation. Using UPLC-Q/TOF, 18 potential candidates were identified according to unique mass spectrometric fragmentation. After in vitro biological evaluation, several indole alkaloids with anti-inflammatory and anti-asthmatic properties were found, including akuammidine, (E)-alstoscholarine, and (Z)-alstoscholarine. Compared with conventional fingerprints, the microfractionation based bioactivity-integrated fingerprints that contain both chemical and bioactivity details offer a more comprehensive understanding of the chemical makeup of plant materials. This strategy clearly demonstrated that dual bioactivity-integrated fingerprinting is a powerful tool for the improved screening and identification of potential dual-target lead compounds in complex herbal medicines. (C) 2012 Elsevier B.V. All rights reserved.
关键词	Dual-bioactivity-integrated Fingerprinting Constituent Identification Nuclear Factor-kappa b Inhibitor Beta(2) Adrenergic Receptor Agonist Ultra Performance Liquid Chromatography/quadrupole Time-of-flight Mass Spectrometry
学科领域	Biochemical Research Methods ; Chemistry, Analytical
DOI	10.1016/j.jchromb.2012.10.004
收录类别	SCI
语种	英语
WOS记录号	WOS:000311195100014
引用统计	被引频次：44[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/15922
专题	植物化学与西部植物资源持续利用国家重点实验室
作者单位	1.Nankai Univ, Coll Pharm, State Key Lab Med Chem Biol, Tianjin 300071, Peoples R China 2.Nankai Univ, Tianjin Key Lab Mol Drug Res, Tianjin 300071, Peoples R China 3.Tianjin Univ, Sch Pharmaceut Sci & Technol, Tianjin 300072, Peoples R China 4.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources W China, Kunming 650204, Yunnan Province, Peoples R China
推荐引用方式 GB/T 7714	Hou, Yuanyuan,Cao, Xuelin,Wang, Liqiang,et al. Microfractionation bioactivity-based ultra performance liquid chromatography/quadrupole time-of-flight mass spectrometry for the identification of nuclear factor-kappa B inhibitors and beta(2) adrenergic receptor agonists in an alkaloidal extract of the folk herb Alstonia scholaris[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2012,908:98-104.
APA	Hou, Yuanyuan.,Cao, Xuelin.,Wang, Liqiang.,Cheng, Binfeng.,Dong, Linyi.,...&Gao, Wenyuan.(2012).Microfractionation bioactivity-based ultra performance liquid chromatography/quadrupole time-of-flight mass spectrometry for the identification of nuclear factor-kappa B inhibitors and beta(2) adrenergic receptor agonists in an alkaloidal extract of the folk herb Alstonia scholaris.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,908,98-104.
MLA	Hou, Yuanyuan,et al."Microfractionation bioactivity-based ultra performance liquid chromatography/quadrupole time-of-flight mass spectrometry for the identification of nuclear factor-kappa B inhibitors and beta(2) adrenergic receptor agonists in an alkaloidal extract of the folk herb Alstonia scholaris".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 908(2012):98-104.

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