Microfractionation bioactivity-based ultra performance liquid chromatography/quadrupole time-of-flight mass spectrometry for the identification of nuclear factor-kappa B inhibitors and beta(2) adrenergic receptor agonists in an alkaloidal extract of the folk herb Alstonia scholaris
Hou, Yuanyuan1,2; Cao, Xuelin3; Wang, Liqiang1,2; Cheng, Binfeng1,2; Dong, Linyi3; Luo, Xiaodong4; Bai, Gang1,2; Gao, Wenyuan3
2012-11-01
发表期刊JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
ISSN1570-0232
卷号908页码:98-104
摘要Traditional Chinese medicines (TCMs) are generally considered complementary or alternative remedies in most Western countries. The constituents of TCMs are hard to define, and their efficacy is difficult to appraise. Thus, the development of suitable methods for evaluating the relationship between bioactivity and the chemical makeup of complex TCM mixtures remains a great challenge. In the present work, the bioactivity-integrated fingerprints of alkaloidal leaf extracts of Alstonia scholaris, a folk medicinal herb for chronic respiratory diseases, were established by ultra performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC-Q/TOF). This method was coupled with two dual-luciferase reporter assay systems to show nuclear factor-kappa B (NF-kappa B) inhibition and beta(2) adrenergic receptor (beta(2)AR) activation. Using UPLC-Q/TOF, 18 potential candidates were identified according to unique mass spectrometric fragmentation. After in vitro biological evaluation, several indole alkaloids with anti-inflammatory and anti-asthmatic properties were found, including akuammidine, (E)-alstoscholarine, and (Z)-alstoscholarine. Compared with conventional fingerprints, the microfractionation based bioactivity-integrated fingerprints that contain both chemical and bioactivity details offer a more comprehensive understanding of the chemical makeup of plant materials. This strategy clearly demonstrated that dual bioactivity-integrated fingerprinting is a powerful tool for the improved screening and identification of potential dual-target lead compounds in complex herbal medicines. (C) 2012 Elsevier B.V. All rights reserved.
关键词Dual-bioactivity-integrated Fingerprinting Constituent Identification Nuclear Factor-kappa b Inhibitor Beta(2) Adrenergic Receptor Agonist Ultra Performance Liquid Chromatography/quadrupole Time-of-flight Mass Spectrometry
资助信息National Natural Science Foundation of China [81173638, 81001682]; Fundamental Research Funds for the Central Universities [65011111]
学科领域Biochemical Research Methods ; Chemistry, Analytical
DOI10.1016/j.jchromb.2012.10.004
收录类别SCI
语种英语
WOS研究方向Biochemistry & Molecular Biology ; Chemistry
WOS类目Biochemical Research Methods ; Chemistry, Analytical
WOS记录号WOS:000311195100014
引用统计
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/15922
专题植物化学与西部植物资源持续利用国家重点实验室
作者单位1.Nankai Univ, Coll Pharm, State Key Lab Med Chem Biol, Tianjin 300071, Peoples R China
2.Nankai Univ, Tianjin Key Lab Mol Drug Res, Tianjin 300071, Peoples R China
3.Tianjin Univ, Sch Pharmaceut Sci & Technol, Tianjin 300072, Peoples R China
4.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources W China, Kunming 650204, Yunnan Province, Peoples R China
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Hou, Yuanyuan,Cao, Xuelin,Wang, Liqiang,et al. Microfractionation bioactivity-based ultra performance liquid chromatography/quadrupole time-of-flight mass spectrometry for the identification of nuclear factor-kappa B inhibitors and beta(2) adrenergic receptor agonists in an alkaloidal extract of the folk herb Alstonia scholaris[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2012,908:98-104.
APA Hou, Yuanyuan.,Cao, Xuelin.,Wang, Liqiang.,Cheng, Binfeng.,Dong, Linyi.,...&Gao, Wenyuan.(2012).Microfractionation bioactivity-based ultra performance liquid chromatography/quadrupole time-of-flight mass spectrometry for the identification of nuclear factor-kappa B inhibitors and beta(2) adrenergic receptor agonists in an alkaloidal extract of the folk herb Alstonia scholaris.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,908,98-104.
MLA Hou, Yuanyuan,et al."Microfractionation bioactivity-based ultra performance liquid chromatography/quadrupole time-of-flight mass spectrometry for the identification of nuclear factor-kappa B inhibitors and beta(2) adrenergic receptor agonists in an alkaloidal extract of the folk herb Alstonia scholaris".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 908(2012):98-104.
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