Microfractionation bioactivity-based ultra performance liquid chromatography/quadrupole time-of-flight mass spectrometry for the identification of nuclear factor-kappa B inhibitors and beta(2) adrenergic receptor agonists in an alkaloidal extract of the folk herb Alstonia scholaris
Hou, Yuanyuan1,2; Cao, Xuelin3; Wang, Liqiang1,2; Cheng, Binfeng1,2; Dong, Linyi3; Luo, Xiaodong4; Bai, Gang1,2; Gao, Wenyuan3
Corresponding AuthorBai, G (reprint author), Nankai Univ, Coll Pharm, State Key Lab Med Chem Biol, Tianjin 300071, Peoples R China.,gangbai@nankai.edu.cn ; pharmgao@tju.edu.cn
2012-11-01
Source PublicationJOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
ISSN1570-0232
Volume908Pages:98-104
AbstractTraditional Chinese medicines (TCMs) are generally considered complementary or alternative remedies in most Western countries. The constituents of TCMs are hard to define, and their efficacy is difficult to appraise. Thus, the development of suitable methods for evaluating the relationship between bioactivity and the chemical makeup of complex TCM mixtures remains a great challenge. In the present work, the bioactivity-integrated fingerprints of alkaloidal leaf extracts of Alstonia scholaris, a folk medicinal herb for chronic respiratory diseases, were established by ultra performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC-Q/TOF). This method was coupled with two dual-luciferase reporter assay systems to show nuclear factor-kappa B (NF-kappa B) inhibition and beta(2) adrenergic receptor (beta(2)AR) activation. Using UPLC-Q/TOF, 18 potential candidates were identified according to unique mass spectrometric fragmentation. After in vitro biological evaluation, several indole alkaloids with anti-inflammatory and anti-asthmatic properties were found, including akuammidine, (E)-alstoscholarine, and (Z)-alstoscholarine. Compared with conventional fingerprints, the microfractionation based bioactivity-integrated fingerprints that contain both chemical and bioactivity details offer a more comprehensive understanding of the chemical makeup of plant materials. This strategy clearly demonstrated that dual bioactivity-integrated fingerprinting is a powerful tool for the improved screening and identification of potential dual-target lead compounds in complex herbal medicines. (C) 2012 Elsevier B.V. All rights reserved.
KeywordDual-bioactivity-integrated Fingerprinting Constituent Identification Nuclear Factor-kappa b Inhibitor Beta(2) Adrenergic Receptor Agonist Ultra Performance Liquid Chromatography/quadrupole Time-of-flight Mass Spectrometry
Subject AreaBiochemical Research Methods ; Chemistry, Analytical
DOI10.1016/j.jchromb.2012.10.004
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS IDWOS:000311195100014
Citation statistics
Cited Times:27[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/15922
Collection植物化学与西部植物资源持续利用国家重点实验室
Affiliation1.Nankai Univ, Coll Pharm, State Key Lab Med Chem Biol, Tianjin 300071, Peoples R China
2.Nankai Univ, Tianjin Key Lab Mol Drug Res, Tianjin 300071, Peoples R China
3.Tianjin Univ, Sch Pharmaceut Sci & Technol, Tianjin 300072, Peoples R China
4.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources W China, Kunming 650204, Yunnan Province, Peoples R China
Recommended Citation
GB/T 7714
Hou, Yuanyuan,Cao, Xuelin,Wang, Liqiang,et al. Microfractionation bioactivity-based ultra performance liquid chromatography/quadrupole time-of-flight mass spectrometry for the identification of nuclear factor-kappa B inhibitors and beta(2) adrenergic receptor agonists in an alkaloidal extract of the folk herb Alstonia scholaris[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2012,908:98-104.
APA Hou, Yuanyuan.,Cao, Xuelin.,Wang, Liqiang.,Cheng, Binfeng.,Dong, Linyi.,...&Gao, Wenyuan.(2012).Microfractionation bioactivity-based ultra performance liquid chromatography/quadrupole time-of-flight mass spectrometry for the identification of nuclear factor-kappa B inhibitors and beta(2) adrenergic receptor agonists in an alkaloidal extract of the folk herb Alstonia scholaris.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,908,98-104.
MLA Hou, Yuanyuan,et al."Microfractionation bioactivity-based ultra performance liquid chromatography/quadrupole time-of-flight mass spectrometry for the identification of nuclear factor-kappa B inhibitors and beta(2) adrenergic receptor agonists in an alkaloidal extract of the folk herb Alstonia scholaris".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 908(2012):98-104.
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