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类黄酮异戊烯转移酶基因在酿酒酵母中的表达
袁田田 ; 高娟 ; 刘小烛
2012
Source Publication安徽农业科学
ISSN0517-6611
Issue18Pages:9596-9597,9600
Contribution Rank第二
Abstract[目的]对类黄酮异戊烯转移酶基因在酿酒酵母中的表达进行研究。[方法]以粗毛淫羊藿为材料,对其类黄酮异戊烯转移酶基因(EaPT1)进行克隆,并构建重组质粒,将其转化至酿酒酵母INVSc1中表达,进行黄酮类底物粗酶检测。[结果]获得了大小约1 200 bp的EaPT1基因;经SDS-PAGE检测,重组质粒转化后的酵母菌有疑似EaPT1的表达产物;粗酶检测中未能检测到EaPT1酶活性。[结论]为粗毛淫羊藿植物次生代谢,特别是类黄酮生物合成途径的解析奠定基础。
Keyword淫羊藿苷:4719 重组质粒:4675 异戊烯转移酶:4630 酿酒酵母:4584 类黄酮:1953 粗毛淫羊藿:1532 基因:1412 酵母细胞:1333 表达产物:1324 表达体系:1311
Indexed Bycscd
Language中文
Citation statistics
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/15342
Collection资源植物与生物技术所级重点实验室
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GB/T 7714
袁田田,高娟,刘小烛. 类黄酮异戊烯转移酶基因在酿酒酵母中的表达[J]. 安徽农业科学,2012(18):9596-9597,9600.
APA 袁田田,高娟,&刘小烛.(2012).类黄酮异戊烯转移酶基因在酿酒酵母中的表达.安徽农业科学(18),9596-9597,9600.
MLA 袁田田,et al."类黄酮异戊烯转移酶基因在酿酒酵母中的表达".安徽农业科学 .18(2012):9596-9597,9600.
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