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题名: Bioactivity-based liquid chromatography-coupled electrospray ionization tandem ion trap/time of flight mass spectrometry for beta(2)AR agonist identification in alkaloidal extract of Alstonia scholaris
作者: Hou, Yuanyuan2, 3, 4; Cao, Xuelin5; Dong, Linyi5; Wang, Liqiang2; Cheng, Binfeng2; Shi, Qian2; null(罗晓东)1; Bai, Gang2, 3, 4
刊名: JOURNAL OF CHROMATOGRAPHY A
关键词: Bioactivity-integrated fingerprinting ; Constituent identification ; beta(2) adrenergic receptor agonist ; LCMS-IT-TOF
英文摘要: Although chromatographic fingerprinting combined with chemometrics, is a rational method for the quality control of traditional Chinese medicine (TCM), chemometrics cannot fully explore the relationship between chemical information and the efficacy of the potential activity. In the present work, a cell-based beta(2) adrenergic receptor (beta(2)AR) agonist functional evaluation model coupled with high-performance liquid chromatography was developed to screen the potential beta(2)AR agonist components in the alkaloidal extract of Alstonia scholaris leaves. Using a liquid chromatography with ion trap time-of-flight mass spectrometry (LCMS-IT-TOF) system, the potential bioactive compounds in the prescription were identified and deduced based on the mass spectrometric fragmentation patterns, tandem mass spectrometry (MS/MS) data, and relevant literature. Several new beta(2)AR agonists of indole alkaloids were successfully found, and their activities were confirmed through an in vivo relaxant test on guinea pig tracheal muscles. The developed method is rapid and reliable compared with conventional fingerprinting and showed high sensitivity and resolution for the identification of beta(2)AR agonists in TCM prescriptions. This strategy clearly demonstrates that bioactivity-integrated fingerprinting is a powerful tool not only in screening and identifying potential lead compounds and in determining the therapeutic material basis of Chinese herbal prescriptions, but also in supplying suitable chemical markers for their quality control. (C) 2012 Elsevier B.V. All rights reserved.
出版日期: 2012-03-02
卷号: 1227, 页码:203-209
DOI标识: 10.1016/j.chroma.2012.01.008
语种: 英语
ISSN号: 0021-9673
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.kib.ac.cn/handle/151853/10377
Appears in Collections:植物化学与西部植物资源持续利用国家重点实验室_期刊论文

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作者单位: 1.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources W China, Kunming 650204, Yunnan Province, Peoples R China
2.Nankai Univ, State Key Lab Med Chem Biol, Tianjin 300071, Peoples R China
3.Nankai Univ, Coll Pharm, Tianjin 300071, Peoples R China
4.Nankai Univ, Tianjin Key Lab Mol Drug Res, Tianjin 300071, Peoples R China
5.Tianjin Univ, Sch Pharmaceut Sci & Technol, Tianjin 300072, Peoples R China

Recommended Citation:
Hou, YY; Cao, XL; Dong, LY; Wang, LQ; Cheng, BF; Shi, Q; Luo, XD; Bai, G.Bioactivity-based liquid chromatography-coupled electrospray ionization tandem ion trap/time of flight mass spectrometry for beta(2)AR agonist identification in alkaloidal extract of Alstonia scholaris,JOURNAL OF CHROMATOGRAPHY A,2012,1227():203-209
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