Bioactivity-based liquid chromatography-coupled electrospray ionization tandem ion trap/time of flight mass spectrometry for beta(2)AR agonist identification in alkaloidal extract of Alstonia scholaris
Hou, Yuanyuan2,3,4; Cao, Xuelin5; Dong, Linyi5; Wang, Liqiang2; Cheng, Binfeng2; Shi, Qian2; Luo, Xiaodong1; Bai, Gang2,3,4
Corresponding AuthorLuo, XD (reprint author), Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources W China, Kunming 650204, Yunnan Province, Peoples R China, xdluo@kib.ac.cn.cn ; gangbai@nankai.edu.cn
2012-03-02
Source PublicationJOURNAL OF CHROMATOGRAPHY A
ISSN0021-9673
Volume1227Pages:203-209
AbstractAlthough chromatographic fingerprinting combined with chemometrics, is a rational method for the quality control of traditional Chinese medicine (TCM), chemometrics cannot fully explore the relationship between chemical information and the efficacy of the potential activity. In the present work, a cell-based beta(2) adrenergic receptor (beta(2)AR) agonist functional evaluation model coupled with high-performance liquid chromatography was developed to screen the potential beta(2)AR agonist components in the alkaloidal extract of Alstonia scholaris leaves. Using a liquid chromatography with ion trap time-of-flight mass spectrometry (LCMS-IT-TOF) system, the potential bioactive compounds in the prescription were identified and deduced based on the mass spectrometric fragmentation patterns, tandem mass spectrometry (MS/MS) data, and relevant literature. Several new beta(2)AR agonists of indole alkaloids were successfully found, and their activities were confirmed through an in vivo relaxant test on guinea pig tracheal muscles. The developed method is rapid and reliable compared with conventional fingerprinting and showed high sensitivity and resolution for the identification of beta(2)AR agonists in TCM prescriptions. This strategy clearly demonstrates that bioactivity-integrated fingerprinting is a powerful tool not only in screening and identifying potential lead compounds and in determining the therapeutic material basis of Chinese herbal prescriptions, but also in supplying suitable chemical markers for their quality control. (C) 2012 Elsevier B.V. All rights reserved.
KeywordBioactivity-integrated Fingerprinting Constituent Identification Beta(2) Adrenergic Receptor Agonist Lcms-it-tof
Subject AreaBiochemical Research Methods ; Chemistry, Analytical
DOI10.1016/j.chroma.2012.01.008
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS IDWOS:000301563700023
Citation statistics
Cited Times:39[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/10377
Collection植物化学与西部植物资源持续利用国家重点实验室
Affiliation1.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources W China, Kunming 650204, Yunnan Province, Peoples R China
2.Nankai Univ, State Key Lab Med Chem Biol, Tianjin 300071, Peoples R China
3.Nankai Univ, Coll Pharm, Tianjin 300071, Peoples R China
4.Nankai Univ, Tianjin Key Lab Mol Drug Res, Tianjin 300071, Peoples R China
5.Tianjin Univ, Sch Pharmaceut Sci & Technol, Tianjin 300072, Peoples R China
Recommended Citation
GB/T 7714
Hou, Yuanyuan,Cao, Xuelin,Dong, Linyi,et al. Bioactivity-based liquid chromatography-coupled electrospray ionization tandem ion trap/time of flight mass spectrometry for beta(2)AR agonist identification in alkaloidal extract of Alstonia scholaris[J]. JOURNAL OF CHROMATOGRAPHY A,2012,1227:203-209.
APA Hou, Yuanyuan.,Cao, Xuelin.,Dong, Linyi.,Wang, Liqiang.,Cheng, Binfeng.,...&Bai, Gang.(2012).Bioactivity-based liquid chromatography-coupled electrospray ionization tandem ion trap/time of flight mass spectrometry for beta(2)AR agonist identification in alkaloidal extract of Alstonia scholaris.JOURNAL OF CHROMATOGRAPHY A,1227,203-209.
MLA Hou, Yuanyuan,et al."Bioactivity-based liquid chromatography-coupled electrospray ionization tandem ion trap/time of flight mass spectrometry for beta(2)AR agonist identification in alkaloidal extract of Alstonia scholaris".JOURNAL OF CHROMATOGRAPHY A 1227(2012):203-209.
Files in This Item: Download All
File Name/Size DocType Version Access License
Hou-2012-Bioactivity(949KB) 开放获取--View Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Hou, Yuanyuan]'s Articles
[Cao, Xuelin]'s Articles
[Dong, Linyi]'s Articles
Baidu academic
Similar articles in Baidu academic
[Hou, Yuanyuan]'s Articles
[Cao, Xuelin]'s Articles
[Dong, Linyi]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Hou, Yuanyuan]'s Articles
[Cao, Xuelin]'s Articles
[Dong, Linyi]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Hou-2012-Bioactivity-based li.pdf
Format: Adobe PDF
This file does not support browsing at this time
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.