Bioactivity-based liquid chromatography-coupled electrospray ionization tandem ion trap/time of flight mass spectrometry for beta(2)AR agonist identification in alkaloidal extract of Alstonia scholaris
Hou, Yuanyuan2,3,4; Cao, Xuelin5; Dong, Linyi5; Wang, Liqiang2; Cheng, Binfeng2; Shi, Qian2; Luo, Xiaodong1; Bai, Gang2,3,4
2012-03-02
发表期刊JOURNAL OF CHROMATOGRAPHY A
ISSN0021-9673
卷号1227页码:203-209
摘要Although chromatographic fingerprinting combined with chemometrics, is a rational method for the quality control of traditional Chinese medicine (TCM), chemometrics cannot fully explore the relationship between chemical information and the efficacy of the potential activity. In the present work, a cell-based beta(2) adrenergic receptor (beta(2)AR) agonist functional evaluation model coupled with high-performance liquid chromatography was developed to screen the potential beta(2)AR agonist components in the alkaloidal extract of Alstonia scholaris leaves. Using a liquid chromatography with ion trap time-of-flight mass spectrometry (LCMS-IT-TOF) system, the potential bioactive compounds in the prescription were identified and deduced based on the mass spectrometric fragmentation patterns, tandem mass spectrometry (MS/MS) data, and relevant literature. Several new beta(2)AR agonists of indole alkaloids were successfully found, and their activities were confirmed through an in vivo relaxant test on guinea pig tracheal muscles. The developed method is rapid and reliable compared with conventional fingerprinting and showed high sensitivity and resolution for the identification of beta(2)AR agonists in TCM prescriptions. This strategy clearly demonstrates that bioactivity-integrated fingerprinting is a powerful tool not only in screening and identifying potential lead compounds and in determining the therapeutic material basis of Chinese herbal prescriptions, but also in supplying suitable chemical markers for their quality control. (C) 2012 Elsevier B.V. All rights reserved.
关键词Bioactivity-integrated Fingerprinting Constituent Identification Beta(2) Adrenergic Receptor Agonist Lcms-it-tof
资助信息National Natural Science Foundation of China[81173638, 81001682]; Fundamental Research Funds for the Central Universities[65011111]
学科领域Biochemical Research Methods ; Chemistry, Analytical
DOI10.1016/j.chroma.2012.01.008
收录类别SCI
语种英语
WOS研究方向Biochemistry & Molecular Biology ; Chemistry
WOS类目Biochemical Research Methods ; Chemistry, Analytical
WOS记录号WOS:000301563700023
引用统计
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/10377
专题植物化学与西部植物资源持续利用国家重点实验室
作者单位1.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources W China, Kunming 650204, Yunnan Province, Peoples R China
2.Nankai Univ, State Key Lab Med Chem Biol, Tianjin 300071, Peoples R China
3.Nankai Univ, Coll Pharm, Tianjin 300071, Peoples R China
4.Nankai Univ, Tianjin Key Lab Mol Drug Res, Tianjin 300071, Peoples R China
5.Tianjin Univ, Sch Pharmaceut Sci & Technol, Tianjin 300072, Peoples R China
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Hou, Yuanyuan,Cao, Xuelin,Dong, Linyi,et al. Bioactivity-based liquid chromatography-coupled electrospray ionization tandem ion trap/time of flight mass spectrometry for beta(2)AR agonist identification in alkaloidal extract of Alstonia scholaris[J]. JOURNAL OF CHROMATOGRAPHY A,2012,1227:203-209.
APA Hou, Yuanyuan.,Cao, Xuelin.,Dong, Linyi.,Wang, Liqiang.,Cheng, Binfeng.,...&Bai, Gang.(2012).Bioactivity-based liquid chromatography-coupled electrospray ionization tandem ion trap/time of flight mass spectrometry for beta(2)AR agonist identification in alkaloidal extract of Alstonia scholaris.JOURNAL OF CHROMATOGRAPHY A,1227,203-209.
MLA Hou, Yuanyuan,et al."Bioactivity-based liquid chromatography-coupled electrospray ionization tandem ion trap/time of flight mass spectrometry for beta(2)AR agonist identification in alkaloidal extract of Alstonia scholaris".JOURNAL OF CHROMATOGRAPHY A 1227(2012):203-209.
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