| Identification and evaluation of apoptotic compounds from Garcinia oligantha | |
Gao, Xue-Mei1,3; Yu, Ting2; Cui, Ming-zhu1,3; Pu, Jian-Xin4 ; Du, Xue4; Han, Quan-bin5; Hu, Qiu-fen1,3; Liu, Tian-Cheng1,3; Luo, Kathy Qian2; Xu, Hong-Xi6
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| 通讯作者 | Gao, XM (reprint author), Yunnan Univ Nationalities, Key Lab Ethn Med Resource Chem, State Ethn Affairs Commiss, 134 Yi Er Yi Ave, Kunming 650031, Yunnan, Peoples R China, gao_xuemei@hotmail.com ; kluo@ntu.edu.sg ; xuhongxi88@gmail.com |
| 2012-03-15 | |
| 发表期刊 | BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
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| ISSN | 0960-894X |
| 卷号 | 22期号:6页码:2350-2353 |
| 摘要 | Four new compounds, oliganthins A-D (1-4), and one known caged xanthone gaudichaudione H (5) were isolated from the stems of Garcinia oligantha. The structures of the new compounds were elucidated by spectroscopic evidences. All of the five compounds were evaluated for their apoptosis-inducing effects using HeLa-C3 cells which have been genetically engineered to produce a fluorescent biosensor capable of detecting caspase-3 activation. All of them induced cell apoptosis at 10 mu M or lower concentrations. The apoptotic activity of oliganthins A, B and gaudichaudione H were further confirmed by detecting the cleavage of PARP, which is the substrate of activated caspase-3, in these compounds-treated cells using the method of Western blot. Moreover, the values of IC50 were measured for all five compounds on HeLa cells using the MTT assay. Among them, gaudichaudione H had the lowest IC50 value of 0.90 mu M, while the other four new compounds had IC50 values of 1.58, 1.52, 4.15, and 7.82 mu M, respectively. These results show that gaudichaudione H has the strongest apoptosis-inducing effect and cell growth inhibition effect among these xanthones and it may have the potential to be developed into a new anticancer agent. (C) 2012 Elsevier Ltd. All rights reserved. |
| 关键词 | Anticancer Drug Garcinia Oligantha Xanthone Apoptosis Caspase-3 |
| 学科领域 | Chemistry, Medicinal ; Chemistry, Organic |
| DOI | 10.1016/j.bmcl.2012.01.068 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000301285500047 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.kib.ac.cn/handle/151853/10365 |
| 专题 | 植物化学与西部植物资源持续利用国家重点实验室 |
| 作者单位 | 1.Yunnan Univ Nationalities, Key Lab Ethn Med Resource Chem, State Ethn Affairs Commiss, Kunming 650031, Yunnan, Peoples R China 2.Nanyang Technol Univ, Div Bioengn, Sch Chem & Biomed Engn, Singapore 637457, Singapore 3.Yunnan Univ Nationalities, Minist Educ, Kunming 650031, Yunnan, Peoples R China 4.Chinese Acad Sci, State Key Lab Phytochem & Plant Resources W China, Kunming Inst Bot, Kunming 650204, Yunnan, Peoples R China 5.Hong Kong Baptist Univ, Sch Chinese Med, Kowloon, Hong Kong, Peoples R China 6.Shanghai Univ Tradit Chinese Med Cai Lun Lu 1200, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gao, Xue-Mei,Yu, Ting,Cui, Ming-zhu,et al. Identification and evaluation of apoptotic compounds from Garcinia oligantha[J]. BIOORGANIC & MEDICINAL CHEMISTRY LETTERS,2012,22(6):2350-2353. |
| APA | Gao, Xue-Mei.,Yu, Ting.,Cui, Ming-zhu.,Pu, Jian-Xin.,Du, Xue.,...&Xu, Hong-Xi.(2012).Identification and evaluation of apoptotic compounds from Garcinia oligantha.BIOORGANIC & MEDICINAL CHEMISTRY LETTERS,22(6),2350-2353. |
| MLA | Gao, Xue-Mei,et al."Identification and evaluation of apoptotic compounds from Garcinia oligantha".BIOORGANIC & MEDICINAL CHEMISTRY LETTERS 22.6(2012):2350-2353. |
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