Identification and evaluation of apoptotic compounds from Garcinia oligantha
Gao, Xue-Mei1,3; Yu, Ting2; Cui, Ming-zhu1,3; Pu, Jian-Xin4; Du, Xue4; Han, Quan-bin5; Hu, Qiu-fen1,3; Liu, Tian-Cheng1,3; Luo, Kathy Qian2; Xu, Hong-Xi6
2012-03-15
发表期刊BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
ISSN0960-894X
卷号22期号:6页码:2350-2353
摘要Four new compounds, oliganthins A-D (1-4), and one known caged xanthone gaudichaudione H (5) were isolated from the stems of Garcinia oligantha. The structures of the new compounds were elucidated by spectroscopic evidences. All of the five compounds were evaluated for their apoptosis-inducing effects using HeLa-C3 cells which have been genetically engineered to produce a fluorescent biosensor capable of detecting caspase-3 activation. All of them induced cell apoptosis at 10 mu M or lower concentrations. The apoptotic activity of oliganthins A, B and gaudichaudione H were further confirmed by detecting the cleavage of PARP, which is the substrate of activated caspase-3, in these compounds-treated cells using the method of Western blot. Moreover, the values of IC50 were measured for all five compounds on HeLa cells using the MTT assay. Among them, gaudichaudione H had the lowest IC50 value of 0.90 mu M, while the other four new compounds had IC50 values of 1.58, 1.52, 4.15, and 7.82 mu M, respectively. These results show that gaudichaudione H has the strongest apoptosis-inducing effect and cell growth inhibition effect among these xanthones and it may have the potential to be developed into a new anticancer agent. (C) 2012 Elsevier Ltd. All rights reserved.
关键词Anticancer Drug Garcinia Oligantha Xanthone Apoptosis Caspase-3
资助信息National Natural Science Foundation of China[21002085, 81073014, 81173485]; Key Laboratory of Ethnic Medicine Resource Chemistry (Yunnan University of Nationalities); State Ethnic Affairs Commission & Ministry of Education[MZY100105]; Yunnan University of Nationalities; Minister of Education in Singapore
学科领域Chemistry, Medicinal ; Chemistry, Organic
DOI10.1016/j.bmcl.2012.01.068
收录类别SCI
语种英语
WOS研究方向Pharmacology & Pharmacy ; Chemistry
WOS类目Chemistry, Medicinal ; Chemistry, Organic
WOS记录号WOS:000301285500047
引用统计
被引频次:22[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/10365
专题植物化学与西部植物资源持续利用国家重点实验室
作者单位1.Yunnan Univ Nationalities, Key Lab Ethn Med Resource Chem, State Ethn Affairs Commiss, Kunming 650031, Yunnan, Peoples R China
2.Nanyang Technol Univ, Div Bioengn, Sch Chem & Biomed Engn, Singapore 637457, Singapore
3.Yunnan Univ Nationalities, Minist Educ, Kunming 650031, Yunnan, Peoples R China
4.Chinese Acad Sci, State Key Lab Phytochem & Plant Resources W China, Kunming Inst Bot, Kunming 650204, Yunnan, Peoples R China
5.Hong Kong Baptist Univ, Sch Chinese Med, Kowloon, Hong Kong, Peoples R China
6.Shanghai Univ Tradit Chinese Med Cai Lun Lu 1200, Shanghai 201203, Peoples R China
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GB/T 7714
Gao, Xue-Mei,Yu, Ting,Cui, Ming-zhu,et al. Identification and evaluation of apoptotic compounds from Garcinia oligantha[J]. BIOORGANIC & MEDICINAL CHEMISTRY LETTERS,2012,22(6):2350-2353.
APA Gao, Xue-Mei.,Yu, Ting.,Cui, Ming-zhu.,Pu, Jian-Xin.,Du, Xue.,...&Xu, Hong-Xi.(2012).Identification and evaluation of apoptotic compounds from Garcinia oligantha.BIOORGANIC & MEDICINAL CHEMISTRY LETTERS,22(6),2350-2353.
MLA Gao, Xue-Mei,et al."Identification and evaluation of apoptotic compounds from Garcinia oligantha".BIOORGANIC & MEDICINAL CHEMISTRY LETTERS 22.6(2012):2350-2353.
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