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中国科学院昆明植物研究所知识管理系统
Knowledge Management System of Kunming Institute of Botany,CAS
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0.05) between wild (AR = 4.651), semi-cultivated (AR = 5.091) and cultivated (AR = 5.132) populations of C. taliensis, which suggested that the genetic background of long-lived woody plant was not easy to be changed, and there were moderate high gene flow between populations. However, there was a significant difference (P < 0.05) between wild (AR = 5.9) and cultivated (AR = 7.1) populations distributed in the same place in Yun county, Yunnan province, which may result from the hybridization and introgression of species in the tea garden and anthropogenic damages to the wild population. The hypothesis of hybrid origin of C. grandibracteata was tested by morphological and microsatellites analyses. Compared with other species, the locules in ovary of C. grandibracteata are variable, which showed a morphological intermediate and mosaic. Except one private allele, Ninety-nine percent alleles of C. grandibracteata were shared with these of C. taliensis and C. sinensis var. assamica. And C. grandibracteata was nested in the cluster of C. taliensis in the UPGMA tree. Conclusively, our results supported the hypothesis of hybrid origin of C. grandibracteata partly. The speciation of C. grandibracteata was derived from hybridization and asymmetrical introgression potentially. It is possible that C. taliensis was one of its parents, but it still needs more evidences to prove that C. sinensis var. assamica was another parent.","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&sort_by=2&isNonaffiliated=false&search_type=-1&query1=Origins&order=desc&&fq=dc.project.title_filter%3ACamellia%5C+taliensis%5C+%5C%28W.%5C+W.%5C+Smith%5C%29%5C+Melchior%2C%5C+a%5C+member%5C+of%5C+Camellia%5C+sect.%5C+Thea%2C%5C+is%5C+an%5C+indigenous%5C+species%5C+in%5C+local%5C+natural%5C+forest%5C+and%5C+has%5C+a%5C+long%5C+cultivative%5C+history%5C+in%5C+western%5C+Yunnan%5C+and%5C+its%5C+neighborhood%2C%5C+where%5C+the%5C+domestications%5C+of%5C+this%5C+species%5C+in%5C+different%5C+historical%5C+periods%5C+and%5C+in%5C+different%5C+ways%5C+can%5C+be%5C+found.%5C+C.%5C+taliensis%5C+is%5C+an%5C+important%5C+contributor%5C+to%5C+the%5C+formations%5C+of%5C+tea%5C+landraces%5C+by%5C+hybridization%5C+and%5C+introgression.%5C+In%5C+the%5C+present%5C+study%2C%5C+14%5C+microsatellite%5C+loci%5C+screened%5C+from%5C+37%5C+loci%5C+were%5C+used%5C+to%5C+explore%5C+the%5C+genetic%5C+diversity%5C+about%5C+this%5C+species%5C+with%5C+579%5C+samples%5C+from%5C+25%5C+populations%5C+%5C%2816%5C+wild%5C+populations%2C%5C+4%5C+semi%5C-cultivated%5C+populations%5C+and%5C+5%5C+cultivated%5C+populations%5C%29.%5C+At%5C+the%5C+same%5C+time%2C%5C+the%5C+potential%5C+hybrid%5C+speciation%5C+of%5C+C.%5C+grandibracteata%2C%5C+was%5C+investigated%5C+using%5C+39%5C+individuals%5C+from%5C+2%5C+populations%2C%5C+along%5C+with%5C+C.%5C+taliensis%5C+and%5C+C.%5C+sinensis%5C+var.%5C+assamica%5C+%5C%2883%5C+individuals%5C+from%5C+4%5C+populations%5C%29%5C+by%5C+the%5C+same%5C+microsatellite%5C+markers.%5C+C.%5C+taliensis%5C+had%5C+a%5C+moderate%5C+high%5C+level%5C+of%5C+genetic%5C+diversity%5C+%5C%28A%5C+%3D%5C+14.3%2C%5C+Ne%3D%5C+5.7%2C%5C+HE%5C+%3D%5C+0.666%2C%5C+I%5C+%3D%5C+1.753%2C%5C+AR%5C+%3D%5C+7.2%2C%5C+PPB%5C+%3D%5C+100%25%5C%29.%5C+This%5C+may%5C+result%5C+from%5C+several%5C+factors%5C+including%5C+K%5C-strategy%2C%5C+genetic%5C+background%2C%5C+gene%5C+flow%5C+between%5C+populations%2C%5C+hybridization%5C+and%5C+introgression%5C+among%5C+species.%5C+Between%5C+wild%5C+populations%5C+of%5C+C.%5C+taliensis%2C%5C+the%5C+gene%5C+flow%5C+was%5C+moderate%5C+high%5C+%5C%28Nm%5C+%3D%5C+1.197%5C%29%2C%5C+and%5C+genetic%5C+variation%5C+was%5C+less%5C+than%5C+20%25%5C+%5C%28GST%5C+%3D%5C+0.147%2C%5C+FST%5C+%3D%5C+0.173%5C%29%2C%5C+which%5C+was%5C+similar%5C+to%5C+other%5C+research%5C+results%5C+of%5C+long%5C-lived%5C+woody%5C+plants%2C%5C+and%5C+reflected%5C+the%5C+genetic%5C+structure%5C+of%5C+its%5C+ancestry%5C+to%5C+same%5C+extent.%5C+There%5C+was%5C+a%5C+high%5C+significant%5C+correlation%5C+between%5C+geographic%5C+distance%5C+and%5C+Nei%E2%80%99s%5C+genetic%5C+distance%5C+%5C%28r%5C+%3D%5C+0.372%2C%5C+P%5C+%3D%5C+0.001%5C%29%5C+of%5C+populations%2C%5C+which%5C+accorded%5C+with%5C+isolation%5C+by%5C+distance%5C+model.%5C+Inferring%5C+from%5C+Bayesian%5C+clustering%5C+of%5C+genotypes%2C%5C+all%5C+individuals%5C+of%5C+C.%5C+taliensis%5C+were%5C+divided%5C+into%5C+two%5C+groups%2C%5C+conflicting%5C+with%5C+the%5C+result%5C+based%5C+on%5C+Nei%E2%80%99s%5C+genetic%5C+distance%5C+and%5C+real%5C+geographic%5C+distribution%2C%5C+which%5C+suggested%5C+there%5C+were%5C+heavy%5C+and%5C+non%5C-random%5C+influences%5C+by%5C+human%5C+practices.%5C+According%5C+to%5C+allelic%5C+richness%2C%5C+there%5C+were%5C+no%5C+significant%5C+differences%5C+%5C%28P%5C+%3E%5C+0.05%5C%29%5C+between%5C+wild%5C+%5C%28AR%5C+%3D%5C+4.651%5C%29%2C%5C+semi%5C-cultivated%5C+%5C%28AR%5C+%3D%5C+5.091%5C%29%5C+and%5C+cultivated%5C+%5C%28AR%5C+%3D%5C+5.132%5C%29%5C+populations%5C+of%5C+C.%5C+taliensis%2C%5C+which%5C+suggested%5C+that%5C+the%5C+genetic%5C+background%5C+of%5C+long%5C-lived%5C+woody%5C+plant%5C+was%5C+not%5C+easy%5C+to%5C+be%5C+changed%2C%5C+and%5C+there%5C+were%5C+moderate%5C+high%5C+gene%5C+flow%5C+between%5C+populations.%5C+However%2C%5C+there%5C+was%5C+a%5C+significant%5C+difference%5C+%5C%28P%5C+%3C%5C+0.05%5C%29%5C+between%5C+wild%5C+%5C%28AR%5C+%3D%5C+5.9%5C%29%5C+and%5C+cultivated%5C+%5C%28AR%5C+%3D%5C+7.1%5C%29%5C+populations%5C+distributed%5C+in%5C+the%5C+same%5C+place%5C+in%5C+Yun%5C+county%2C%5C+Yunnan%5C+province%2C%5C+which%5C+may%5C+result%5C+from%5C+the%5C+hybridization%5C+and%5C+introgression%5C+of%5C+species%5C+in%5C+the%5C+tea%5C+garden%5C+and%5C+anthropogenic%5C+damages%5C+to%5C+the%5C+wild%5C+population.%5C+The%5C+hypothesis%5C+of%5C+hybrid%5C+origin%5C+of%5C+C.%5C+grandibracteata%5C+was%5C+tested%5C+by%5C+morphological%5C+and%5C+microsatellites%5C+analyses.%5C+Compared%5C+with%5C+other%5C+species%2C%5C+the%5C+locules%5C+in%5C+ovary%5C+of%5C+C.%5C+grandibracteata%5C+are%5C+variable%2C%5C+which%5C+showed%5C+a%5C+morphological%5C+intermediate%5C+and%5C+mosaic.%5C+Except%5C+one%5C+private%5C+allele%2C%5C+Ninety%5C-nine%5C+percent%5C+alleles%5C+of%5C+C.%5C+grandibracteata%5C+were%5C+shared%5C+with%5C+these%5C+of%5C+C.%5C+taliensis%5C+and%5C+C.%5C+sinensis%5C+var.%5C+assamica.%5C+And%5C+C.%5C+grandibracteata%5C+was%5C+nested%5C+in%5C+the%5C+cluster%5C+of%5C+C.%5C+taliensis%5C+in%5C+the%5C+UPGMA%5C+tree.%5C+Conclusively%2C%5C+our%5C+results%5C+supported%5C+the%5C+hypothesis%5C+of%5C+hybrid%5C+origin%5C+of%5C+C.%5C+grandibracteata%5C+partly.%5C+The%5C+speciation%5C+of%5C+C.%5C+grandibracteata%5C+was%5C+derived%5C+from%5C+hybridization%5C+and%5C+asymmetrical%5C+introgression%5C+potentially.%5C+It%5C+is%5C+possible%5C+that%5C+C.%5C+taliensis%5C+was%5C+one%5C+of%5C+its%5C+parents%2C%5C+but%5C+it%5C+still%5C+needs%5C+more%5C+evidences%5C+to%5C+prove%5C+that%5C+C.%5C+sinensis%5C+var.%5C+assamica%5C+was%5C+another%5C+parent."},{"jsname":"China Postdoctoral Science Foundation","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&sort_by=2&isNonaffiliated=false&search_type=-1&query1=Origins&order=desc&&fq=dc.project.title_filter%3AChina%5C+Postdoctoral%5C+Science%5C+Foundation"},{"jsname":"China Scholarship Council","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&sort_by=2&isNonaffiliated=false&search_type=-1&query1=Origins&order=desc&&fq=dc.project.title_filter%3AChina%5C+Scholarship%5C+Council"},{"jsname":"Construction Program of Biology First-class Discipline in Guizhou[CINYL [2017] 009]","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&sort_by=2&isNonaffiliated=false&search_type=-1&query1=Origins&order=desc&&fq=dc.project.title_filter%3AConstruction%5C+Program%5C+of%5C+Biology%5C+First%5C-class%5C+Discipline%5C+in%5C+Guizhou%5C%5BCINYL%5C+%5C%5B2017%5C%5D%5C+009%5C%5D"},{"jsname":"Cyatheaceae species, usually called tree ferns, are considered as relicts of a time when dinosaurs were common. In recent several decades, the number of Cyatheaceae plants decreases dramatically. In order to find the reasons and provide directions for protecting these endangered plants, the biological characteristics of Cyatheaceae were surveyed. Using AFLP and cpDNA sequence variations, the genetic diversity and phylogeography of Sphaeropteris brunoniana were also analyzed. Based on these findings, implications for conservation strategies were discussed for this relict tree fern. Main results of the dissertation were summarized as follows, (1) Cyatheaceae plants have extensive distribution in Yunnan, China, and most of them distribute in southeast of Yunnan. In southeast, they usually inhabit margins of evergreen broad-leaved forests or secondary coniferous forests; however, the population update is very different and the age structure is unscientific. The spore of Cyatheaceae is trilete, radially symmetrical, and perinous. The spores of Alsophila species feature a ridged perine and a granular, verrucate or smooth exine. The spores of S. brunoniana are characterized by an incipient granular outermost layer and a verrucate exine. The metaphase chromosome numbers of gametophytes in the three examined species, viz. A. podophylla, A. gigantea and A. austro-yunnanensis, are 69, indicating that they are diploid and do not display variety in chromosome number. The chemical constituents of S. brunoniana are main simple and familiar compounds, such as saccharides, fatty acids and alcohols, and stigmasterols. (2) An unexpectedly high level of nDNA genetic diversity and low cpDNA diversity were detected in S. brunoniana. (3) This study showed that the genetic differentiation among populations within regions was low and between regions was significant. (4) There were several refugia of S. brunoniana in Yunnan during glacial periods. The Hainan populations were likely new colonizations and originated from Southeast Asia. (5) To retain existing genetic diversity, whether in situ or ex situ conservation or collection of germplasm is used, the populations of the two regions should be considered equally. Furthermore, ex situ conservation of this species should be preferably conducted on large populations.","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&sort_by=2&isNonaffiliated=false&search_type=-1&query1=Origins&order=desc&&fq=dc.project.title_filter%3ACyatheaceae%5C+species%2C%5C+usually%5C+called%5C+tree%5C+ferns%2C%5C+are%5C+considered%5C+as%5C+relicts%5C+of%5C+a%5C+time%5C+when%5C+dinosaurs%5C+were%5C+common.%5C+In%5C+recent%5C+several%5C+decades%2C%5C+the%5C+number%5C+of%5C+Cyatheaceae%5C+plants%5C+decreases%5C+dramatically.%5C+In%5C+order%5C+to%5C+find%5C+the%5C+reasons%5C+and%5C+provide%5C+directions%5C+for%5C+protecting%5C+these%5C+endangered%5C+plants%2C%5C+the%5C+biological%5C+characteristics%5C+of%5C+Cyatheaceae%5C+were%5C+surveyed.%5C+Using%5C+AFLP%5C+and%5C+cpDNA%5C+sequence%5C+variations%2C%5C+the%5C+genetic%5C+diversity%5C+and%5C+phylogeography%5C+of%5C+Sphaeropteris%5C+brunoniana%5C+were%5C+also%5C+analyzed.%5C+Based%5C+on%5C+these%5C+findings%2C%5C+implications%5C+for%5C+conservation%5C+strategies%5C+were%5C+discussed%5C+for%5C+this%5C+relict%5C+tree%5C+fern.%5C+Main%5C+results%5C+of%5C+the%5C+dissertation%5C+were%5C+summarized%5C+as%5C+follows%2C%5C+%5C%281%5C%29%5C+Cyatheaceae%5C+plants%5C+have%5C+extensive%5C+distribution%5C+in%5C+Yunnan%2C%5C+China%2C%5C+and%5C+most%5C+of%5C+them%5C+distribute%5C+in%5C+southeast%5C+of%5C+Yunnan.%5C+In%5C+southeast%2C%5C+they%5C+usually%5C+inhabit%5C+margins%5C+of%5C+evergreen%5C+broad%5C-leaved%5C+forests%5C+or%5C+secondary%5C+coniferous%5C+forests%5C%3B%5C+however%2C%5C+the%5C+population%5C+update%5C+is%5C+very%5C+different%5C+and%5C+the%5C+age%5C+structure%5C+is%5C+unscientific.%5C+The%5C+spore%5C+of%5C+Cyatheaceae%5C+is%5C+trilete%2C%5C+radially%5C+symmetrical%2C%5C+and%5C+perinous.%5C+The%5C+spores%5C+of%5C+Alsophila%5C+species%5C+feature%5C+a%5C+ridged%5C+perine%5C+and%5C+a%5C+granular%2C%5C+verrucate%5C+or%5C+smooth%5C+exine.%5C+The%5C+spores%5C+of%5C+S.%5C+brunoniana%5C+are%5C+characterized%5C+by%5C+an%5C+incipient%5C+granular%5C+outermost%5C+layer%5C+and%5C+a%5C+verrucate%5C+exine.%5C+The%5C+metaphase%5C+chromosome%5C+numbers%5C+of%5C+gametophytes%5C+in%5C+the%5C+three%5C+examined%5C+species%2C%5C+viz.%5C+A.%5C+podophylla%2C%5C+A.%5C+gigantea%5C+and%5C+A.%5C+austro%5C-yunnanensis%2C%5C+are%5C+69%2C%5C+indicating%5C+that%5C+they%5C+are%5C+diploid%5C+and%5C+do%5C+not%5C+display%5C+variety%5C+in%5C+chromosome%5C+number.%5C+The%5C+chemical%5C+constituents%5C+of%5C+S.%5C+brunoniana%5C+are%5C+main%5C+simple%5C+and%5C+familiar%5C+compounds%2C%5C+such%5C+as%5C+saccharides%2C%5C+fatty%5C+acids%5C+and%5C+alcohols%2C%5C+and%5C+stigmasterols.%5C+%5C%282%5C%29%5C+An%5C+unexpectedly%5C+high%5C+level%5C+of%5C+nDNA%5C+genetic%5C+diversity%5C+and%5C+low%5C+cpDNA%5C+diversity%5C+were%5C+detected%5C+in%5C+S.%5C+brunoniana.%5C+%5C%283%5C%29%5C+This%5C+study%5C+showed%5C+that%5C+the%5C+genetic%5C+differentiation%5C+among%5C+populations%5C+within%5C+regions%5C+was%5C+low%5C+and%5C+between%5C+regions%5C+was%5C+significant.%5C+%5C%284%5C%29%5C+There%5C+were%5C+several%5C+refugia%5C+of%5C+S.%5C+brunoniana%5C+in%5C+Yunnan%5C+during%5C+glacial%5C+periods.%5C+The%5C+Hainan%5C+populations%5C+were%5C+likely%5C+new%5C+colonizations%5C+and%5C+originated%5C+from%5C+Southeast%5C+Asia.%5C+%5C%285%5C%29%5C+To%5C+retain%5C+existing%5C+genetic%5C+diversity%2C%5C+whether%5C+in%5C+situ%5C+or%5C+ex%5C+situ%5C+conservation%5C+or%5C+collection%5C+of%5C+germplasm%5C+is%5C+used%2C%5C+the%5C+populations%5C+of%5C+the%5C+two%5C+regions%5C+should%5C+be%5C+considered%5C+equally.%5C+Furthermore%2C%5C+ex%5C+situ%5C+conservation%5C+of%5C+this%5C+species%5C+should%5C+be%5C+preferably%5C+conducted%5C+on%5C+large%5C+populations."},{"jsname":"Cycas micholitzii complex is composed of 5 species: C. micholitzii Dyer, C. bifida (Dyer) K. D. Hill,C. longipetiolula D. Y. Wang, C. debaoensis Y. C. Zhong et C J. Chen, C. multipinnata C J. Chen et S. Y. Yang，and distributed from southwest China to central Vietnam and eastern Laos. Based on sequence data from two maternally inherited cpDNA and one biparentally nuclear DNA fragments, our study revealed the population genetic structure of C. micholitzii complex and explored the potential causes. The evolutionary and demographic histories were investigated. The genetic relationship among species in the complex was also clarified.The results were summarized as follows: 1. Phylogeographic analysis based on chloroplast sequences，We examined chloroplast sequence variation of the atpB-rbcLand psbA-trnHintergenic spacers in 27 populations of C. micholitzii complex, recovering 26 haplotypes. The average within-population diversity (HS = 0.140) was low while total diversity (HT = 0.911) was high. Population differentiation was also high(GST = 0.846, NST = 0.919), indicating significant phylogeographical structure (NST > GST,p < 0.001) and low levels of seed-based gene flow. C. debaoensis (Cycadaceae) is an endangered species restricted to the border of Guangxi and Yunnan province in southwest China. This species has been classified into two types: sand and karst, according to the soil matrix they grow on. We examined chloroplast sequence variation of the cpDNA sequences from 11 populations of this species. Significant population genetic differentiation was detected (GST= 0.684 and FST = 0.74160). There was marked genetic differentiation between populations in the sand and karst regions and no expansion was detected. Climate changes during glacial periods have had significant effects on the current distribution of cycads. The molecular phylogenetic data, together with the geographic distribution of the haplotypes, suggest that C. debaoensis experienced range contraction during glacial periods, and that the current populations are still confined to the original refugia in southwest China which have favorable habitats in glacial period. These results imply that small refugia were maintained in both sand and karst regions during the LGM (last glacial maximum). This species had no postglacial recolonization and only stayed in these refugia up to now. The low within-population diversity of C. debaoensis suggests that there were strong bottleneck events or founder effects within each separate region during the Quaternary climatic oscillations. Relatively high genetic and haplotype diversities were detected in the newly discovered populations, which located at intermediate locality of sand regions and had morphological variation; this is probably the consequence of the admixture of different haplotypes colonizing the area from separate sources. C. micholitzii occurs in the Annan Highlands in central Vietnam near the Laos border. C. bifida occurs in North Vietnam; its distribution extends across the border into adjacent localities in Guangxi and Yunnan in China. For the comparability between them,theywere considered as the same species C. micholitzii by many academicians. The cpDNA sequences from 11 populations showed that these very controversial species, C. micholitzii and C. bifida, is paraphyletic and should belong to the same species C. micholitzii. AMOVA analysis showed that the component of among-population within region/species (76.46%) was unexpectedly larger than the among-species/region component (14.97%), which also indicates that there is no justification for recognizing two species as C. micholitzii and C. bifida. This hypothesis was also supported by the geological data, especially the neotectonic history of the indo-china block, which started to move south since Oligocene and cause the geographic isolation of these two groups. Therefore, the most likely explanation to the phenotypic similarities between these two groups may be the retention of ancestral polymorphisms in the paraphyletic group due to incomplete lineage sorting. Furthermore, the similarities may also be ascribed to pollen-mediated gene flow among geographically proximate populations and/or phenotypic convergence under similar selection schemes in the same region. C.micholitzi had the higest genetic diversity (HT = 0.980,) and genetic differentiation (GST = 0.830, NST = 0.915) among the C. micholitzii complex. The high genetic diversity might be attributed to its long evolutionary history, highly diverse habitats. The ineffective mode of seed dispersal and dramatic neotectonic movement in the distribution range of this species could result in the high genetic differentiation. 2. Phylogeographic analysis based on nuclear ribosomal sequences, We sequenced the nrDNA ITS in all 27 populations sampled, 7 haplotypes were identified, among which C. micholitzii had 6, while C. multipinnata, C. longipetiolula and C. debaoensis shared the remaining one. Compared to chloroplast genes, nuclear genes had higher correlation between genetic and geographical distance, but lower interspecies differentiation (54.42% vs 25.24%). Phylogeographical structure of C. micholitzii and C.bifida based on ITS Variation was consistent with the morphology differentiation. This similar in nuclear gene should be ascribed to pollen-mediated gene flow among geographically proximate populations.Long-distance gene flow over the two groups was clearly interrupted, which brought on the nrDNA genetic differenciation between the geographically isolated groups, to a certain extent affected the morphological variation. 3. Interspecies relationships among Cycas micholitzii complex, We analysed chloroplast sequence variation of the atpB-rbcL and psbA-trnH intergenic spacers in 27 populations sampled of C. micholitzii complex, AMOVA analysis showed that the component of among-species/region component (59.21%). However, phylogenic analysis showed that the haplotypes of C. micholitzii complex couldn`t grouped into four clusters closely corresponding to the narrowly defined C. micholitzi, C. multipinnata, C. debaoensis and C. longipetiolula. We concluded that the conflict may result from several factors: firstly incomplete lineage sorting of C. micholitzii; secondly hybridization/introgression of sympatrically cycads, which would be supported by evidence base on nrDNA ITS sequences; thirdly intramolecular recombination in cpDNA of cycads; eventually the neotectonic movement in the distribution range of this species.","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&sort_by=2&isNonaffiliated=false&search_type=-1&query1=Origins&order=desc&&fq=dc.project.title_filter%3ACycas%5C+micholitzii%5C+complex%5C+is%5C+composed%5C+of%5C+5%5C+species%5C%3A%5C+C.%5C+micholitzii%5C+Dyer%2C%5C+C.%5C+bifida%5C+%5C%28Dyer%5C%29%5C+K.%5C+D.%5C+Hill%2CC.%5C+longipetiolula%5C+D.%5C+Y.%5C+Wang%2C%5C+C.%5C+debaoensis%5C+Y.%5C+C.%5C+Zhong%5C+et%5C+C%5C+J.%5C+Chen%2C%5C+C.%5C+multipinnata%5C+C%5C+J.%5C+Chen%5C+et%5C+S.%5C+Y.%5C+Yang%EF%BC%8Cand%5C+distributed%5C+from%5C+southwest%5C+China%5C+to%5C+central%5C+Vietnam%5C+and%5C+eastern%5C+Laos.%5C+Based%5C+on%5C+sequence%5C+data%5C+from%5C+two%5C+maternally%5C+inherited%5C+cpDNA%5C+and%5C+one%5C+biparentally%5C+nuclear%5C+DNA%5C+fragments%2C%5C+our%5C+study%5C+revealed%5C+the%5C+population%5C+genetic%5C+structure%5C+of%5C+C.%5C+micholitzii%5C+complex%5C+and%5C+explored%5C+the%5C+potential%5C+causes.%5C+The%5C+evolutionary%5C+and%5C+demographic%5C+histories%5C+were%5C+investigated.%5C+The%5C+genetic%5C+relationship%5C+among%5C+species%5C+in%5C+the%5C+complex%5C+was%5C+also%5C+clarified.The%5C+results%5C+were%5C+summarized%5C+as%5C+follows%5C%3A%5C+1.%5C+Phylogeographic%5C+analysis%5C+based%5C+on%5C+chloroplast%5C+sequences%EF%BC%8CWe%5C+examined%5C+chloroplast%5C+sequence%5C+variation%5C+of%5C+the%5C+atpB%5C-rbcLand%5C+psbA%5C-trnHintergenic%5C+spacers%5C+in%5C+27%5C+populations%5C+of%5C+C.%5C+micholitzii%5C+complex%2C%5C+recovering%5C+26%5C+haplotypes.%5C+The%5C+average%5C+within%5C-population%5C+diversity%5C+%5C%28HS%5C+%3D%5C+0.140%5C%29%5C+was%5C+low%5C+while%5C+total%5C+diversity%5C+%5C%28HT%5C+%3D%5C+0.911%5C%29%5C+was%5C+high.%5C+Population%5C+differentiation%5C+was%5C+also%5C+high%5C%28GST%5C+%3D%5C+0.846%2C%5C+NST%5C+%3D%5C+0.919%5C%29%2C%5C+indicating%5C+significant%5C+phylogeographical%5C+structure%5C+%5C%28NST%5C+%3E%5C+GST%2Cp%5C+%3C%5C+0.001%5C%29%5C+and%5C+low%5C+levels%5C+of%5C+seed%5C-based%5C+gene%5C+flow.%5C+C.%5C+debaoensis%5C+%5C%28Cycadaceae%5C%29%5C+is%5C+an%5C+endangered%5C+species%5C+restricted%5C+to%5C+the%5C+border%5C+of%5C+Guangxi%5C+and%5C+Yunnan%5C+province%5C+in%5C+southwest%5C+China.%5C+This%5C+species%5C+has%5C+been%5C+classified%5C+into%5C+two%5C+types%5C%3A%5C+sand%5C+and%5C+karst%2C%5C+according%5C+to%5C+the%5C+soil%5C+matrix%5C+they%5C+grow%5C+on.%5C+We%5C+examined%5C+chloroplast%5C+sequence%5C+variation%5C+of%5C+the%5C+cpDNA%5C+sequences%5C+from%5C+11%5C+populations%5C+of%5C+this%5C+species.%5C+Significant%5C+population%5C+genetic%5C+differentiation%5C+was%5C+detected%5C+%5C%28GST%3D%5C+0.684%5C+and%5C+FST%5C+%3D%5C+0.74160%5C%29.%5C+There%5C+was%5C+marked%5C+genetic%5C+differentiation%5C+between%5C+populations%5C+in%5C+the%5C+sand%5C+and%5C+karst%5C+regions%5C+and%5C+no%5C+expansion%5C+was%5C+detected.%5C+Climate%5C+changes%5C+during%5C+glacial%5C+periods%5C+have%5C+had%5C+significant%5C+effects%5C+on%5C+the%5C+current%5C+distribution%5C+of%5C+cycads.%5C+The%5C+molecular%5C+phylogenetic%5C+data%2C%5C+together%5C+with%5C+the%5C+geographic%5C+distribution%5C+of%5C+the%5C+haplotypes%2C%5C+suggest%5C+that%5C+C.%5C+debaoensis%5C+experienced%5C+range%5C+contraction%5C+during%5C+glacial%5C+periods%2C%5C+and%5C+that%5C+the%5C+current%5C+populations%5C+are%5C+still%5C+confined%5C+to%5C+the%5C+original%5C+refugia%5C+in%5C+southwest%5C+China%5C+which%5C+have%5C+favorable%5C+habitats%5C+in%5C+glacial%5C+period.%5C+These%5C+results%5C+imply%5C+that%5C+small%5C+refugia%5C+were%5C+maintained%5C+in%5C+both%5C+sand%5C+and%5C+karst%5C+regions%5C+during%5C+the%5C+LGM%5C+%5C%28last%5C+glacial%5C+maximum%5C%29.%5C+This%5C+species%5C+had%5C+no%5C+postglacial%5C+recolonization%5C+and%5C+only%5C+stayed%5C+in%5C+these%5C+refugia%5C+up%5C+to%5C+now.%5C+The%5C+low%5C+within%5C-population%5C+diversity%5C+of%5C+C.%5C+debaoensis%5C+suggests%5C+that%5C+there%5C+were%5C+strong%5C+bottleneck%5C+events%5C+or%5C+founder%5C+effects%5C+within%5C+each%5C+separate%5C+region%5C+during%5C+the%5C+Quaternary%5C+climatic%5C+oscillations.%5C+Relatively%5C+high%5C+genetic%5C+and%5C+haplotype%5C+diversities%5C+were%5C+detected%5C+in%5C+the%5C+newly%5C+discovered%5C+populations%2C%5C+which%5C+located%5C+at%5C+intermediate%5C+locality%5C+of%5C+sand%5C+regions%5C+and%5C+had%5C+morphological%5C+variation%5C%3B%5C+this%5C+is%5C+probably%5C+the%5C+consequence%5C+of%5C+the%5C+admixture%5C+of%5C+different%5C+haplotypes%5C+colonizing%5C+the%5C+area%5C+from%5C+separate%5C+sources.%5C+%5C+C.%5C+micholitzii%5C+occurs%5C+in%5C+the%5C+Annan%5C+Highlands%5C+in%5C+central%5C+Vietnam%5C+near%5C+the%5C+Laos%5C+border.%5C+C.%5C+bifida%5C+occurs%5C+in%5C+North%5C+Vietnam%5C%3B%5C+its%5C+distribution%5C+extends%5C+across%5C+the%5C+border%5C+into%5C+adjacent%5C+localities%5C+in%5C+Guangxi%5C+and%5C+Yunnan%5C+in%5C+China.%5C+For%5C+the%5C+comparability%5C+between%5C+them%2Ctheywere%5C+considered%5C+as%5C+the%5C+same%5C+species%5C+C.%5C+micholitzii%5C+by%5C+many%5C+academicians.%5C+T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Systematics and Biogeography of Aralia L. (Araliaceae):Revision of Aralia Sects. Aralia, Humiles, Nanae, andSciadodendron
期刊论文
出版物, 3111, 卷号: 57, 期号: 0, 页码: 1-172
作者:
Jun Wen
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提交时间：2017/07/24
Aralia
Aralia Sect. Aralia
Aralia Sect. Dimorphanthus
Aralia Sect. Humiles
Aralia Sect. Nanae
Aralia Sect. pentapanax
Aralia Sect. Sciadodendron
Biogeography
Araliaceae
Systematics
The Forest Filter Effect vs. Cold Trapping Effect on the AltitudinalDistribution of PCBs: A Case Study of Mt. Gongga, Eastern Tibetan Plateau
期刊论文
出版物, 3111, 期号: 0, 页码: 1-32
作者:
Xin Liu
;
Jun Li
;
Qian Zheng
;
Haijian Bing
;
Ruijie Zhang
;
Yan Wang
;
Chunling Luo
;
Xiang Liu
;
Yanhong Wu
;
Suhong Pan
;
Gan Zhang
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提交时间：2017/07/24
Fundamental issues insystems biology
期刊论文
Problems & paradigms, 3111, 页码: 1270–1276
作者:
Sayaka Taji
;
Takeshi Yamada
;
Yasuko In
;
Shun-ichi Wada
;
Yoshihide Usami
;
Kazuo Sakuma
;
Reiko Tanaka
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提交时间：2017/07/19
Using Dali for Structural Comparison of Proteins
期刊论文
Current Protocols in Bioinformatics, 3111, 期号: 0, 页码: 1-24
作者:
Zuo Z(作者)
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Data Analysisin Vegetation Ecology
期刊论文
出版物, 3111, 期号: 0, 页码: 1-297
作者:
Otto Wildi
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提交时间：2017/07/24
Genomic and structural basis for evolution of tropane alkaloid biosynthesis
期刊论文
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2023, 卷号: 120, 期号: 17, 页码: e2302448120
作者:
Wang,Yong-Jiang
;
Tain,Tian
;
Yu,Jia-Yi
;
Li,Jie
;
Xu,Bingyan
;
Chen,Jianghua
;
D'Auria,John C.
;
Huang,Jian-Ping
;
Huang,Sheng-Xiong
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提交时间：2024/05/09
tropane alkaloids
evolution
biosynthesis
genome
structural biology
METHYLTRANSFERASE
REVEAL
The Sino-Himalayan flora evolved from lowland biomes dominated by tropical floristic elements
期刊论文
BMC BIOLOGY, 2023, 卷号: 21, 期号: 1, 页码: 239
作者:
Liu,Yun
;
Lai,Yang-Jun
;
Ye,Jian-Fei
;
Hu,Hai-Hua
;
Peng,Dan-Xiao
;
Lu,Li-Min
;
Sun,Hang
;
Chen,Zhi-Duan
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浏览/下载：3/0
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提交时间：2024/05/09
Asian monsoon
Endemism
Evolutionary history
Floristic elements
Assembly process
Uplift of Himalaya
Mountain biodiversity
Sino-Himalayan flora
EOCENE PALYNOLOGICAL RECORD
TIBETAN PLATEAU
EVOLUTIONARY HISTORY
HENGDUAN MOUNTAINS
GLOBAL PATTERNS
ASIAN MONSOONS
BIODIVERSITY
CLIMATE
DIVERSITY
DIVERSIFICATION
Clonal Propagation and Assessment of Biomass Production and Saponin Content of Elite Accessions of Wild Paris polyphylla var. yunnanensis
期刊论文
PLANTS-BASEL, 2023, 卷号: 12, 期号: 16, 页码: 2983
作者:
Wang,Mulan
;
Li,Weiqi
;
Qiang,Qi
;
Ma,Junchao
;
Chen,Jiaqi
;
Zhang,Xudong
;
Jia,Yanxia
;
Zhang,Tie
;
Lin,Liang
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浏览/下载：5/0
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提交时间：2024/05/09
medicinal plant
Paris polyphylla var. yunnanesis
somatic embryogenesis
premium cultivars
PLANT-REGENERATION
CONSERVATION
Distinctive plastome evolution in carnivorous angiosperms
期刊论文
BMC PLANT BIOLOGY, 2023, 卷号: 23, 期号: 1, 页码: 660
作者:
Fu,Chao-Nan
;
Wicke,Susann
;
Zhu,An-Dan
;
Li,De-Zhu
;
Gao,Lian-Ming
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浏览/下载：5/2
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提交时间：2024/05/09
Carnivory
Plastome
Substitution rates
Prey-derived nutrient utilization
CHLOROPLAST GENOME
PHYLOGENETIC ANALYSIS
DIGESTIVE MUTUALISM
PARASITIC PLANTS
SEQUENCE
GENES
COEVOLUTION
ALIGNMENT
INSIGHTS
ORGANIZATION
报春花属植物交配系统转变研究
学位论文
: 中国科学院大学, 2022
作者:
钟莉
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提交时间：2024/05/14
报春花属，交配系统，二型花柱，同型花柱，自交综合征，遗传多样性，性状演化
Primula, mating system, distyly, homostyly, genetic diversity, selfing syndrome, trait evolution
