Clonal Propagation and Assessment of Biomass Production and Saponin Content of Elite Accessions of Wild Paris polyphylla var. yunnanensis

doi:10.3390/plants12162983

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	Clonal Propagation and Assessment of Biomass Production and Saponin Content of Elite Accessions of Wild Paris polyphylla var. yunnanensis
	Wang,Mulan; Li,Weiqi; Qiang,Qi; Ma,Junchao; Chen,Jiaqi; Zhang,Xudong; Jia,Yanxia; Zhang,Tie; Lin,Liang
	2023
发表期刊	PLANTS-BASEL
ISSN	2223-7747
卷号	12 期号:16 页码:2983
摘要	Paris polyphylla var. yunnanensis is an endangered medicinal plant endemic to China with great economic importance for the pharmaceutical industry. Two significant barriers to its commercial development are the long duration of its seed germination and the frequency of interspecific hybridization. We developed a method for clonal propagation of Paris polyphylla var. yunnanensis and successfully applied it to selected elite wild plants, which could become cultivar candidates based on their biomass production and saponin content. In comparison to the traditional method, somatic embryogenesis produced an average of 63 somatic embryos per gram of callus in just six weeks, saving 12 to 15 months in plantlet production. The produced in vitro plantlets were strong and healthy and 94% survived transplanting to soil. Using this method, four candidate cultivars with diverse morphologies and geographic origins were clonally reproduced from selected elite wild accessions. In comparison to those obtained with the traditional P. polyphylla propagation technique, they accumulated higher biomass and polyphyllin levels in rhizomes plus adventitious roots during a five-year period. In conclusion, somatic embryogenesis-based methods offer an alternate approach for the rapid and scaled-up production of P. polyphylla, as well as opening up species conservation options.
关键词	medicinal plant Paris polyphylla var. yunnanesis somatic embryogenesis premium cultivars PLANT-REGENERATION CONSERVATION
DOI	10.3390/plants12162983
WOS记录号	WOS:001056541400001
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/75051
专题	中国科学院昆明植物研究所
推荐引用方式 GB/T 7714	Wang,Mulan,Li,Weiqi,Qiang,Qi,et al. Clonal Propagation and Assessment of Biomass Production and Saponin Content of Elite Accessions of Wild Paris polyphylla var. yunnanensis[J]. PLANTS-BASEL,2023,12(16):2983.
APA	Wang,Mulan.,Li,Weiqi.,Qiang,Qi.,Ma,Junchao.,Chen,Jiaqi.,...&Lin,Liang.(2023).Clonal Propagation and Assessment of Biomass Production and Saponin Content of Elite Accessions of Wild Paris polyphylla var. yunnanensis.PLANTS-BASEL,12(16),2983.
MLA	Wang,Mulan,et al."Clonal Propagation and Assessment of Biomass Production and Saponin Content of Elite Accessions of Wild Paris polyphylla var. yunnanensis".PLANTS-BASEL 12.16(2023):2983.

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