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中国科学院昆明植物研究所知识管理系统
Knowledge Management System of Kunming Institute of Botany,CAS
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GST, p < 0.01). At the regional level, Chinese and Japanese L. hodgsonii had a similar estimate of genetic diversity (China: Hd = 0.847, HT = 0.869; Japan: Hd = 0.766, HT = 0.867). Populations from China and Japan possess unique sets of haplotypes, and no haplotypes were shared between the regions. Furthermore, both the phyloegenetic and network analyses recovered the haplotypes of China and Japan as two distinct clades. Thus, we suggested the disjunct distribution of L. hodgsonii in China and Japan may present the climatic vicariant relicts of the ancient widely distributed populations. After divergence, this species within each region experienced independent evolutionary process. In China, L. hodgsonii was distributed around the Sichuan Basin. This distribution range can be divided into five regions. They were Jiajin Mountain region, E’mei Mountain region, Yunnan-Guizhou Plateau region, Wushan-Wuling Mountain region and Qinling Mountain region. Twelve haplotypes were indentified within these regions. Each region had its own specific haplotypes, which had different ancestry in the network. We deduced that Chinese L. hodgsonii might survive the LGM in multiple isolated refugia around the Sichuan Basin. In Japan, L. hodgsonii was disjunctively distributed in northern Honshu and Hokkaido. Seven haplotypes were identified within this region. However, the genetic diversity in Honshu (Hd = 0.821) was much higher than that in Hokkaido (Hd = 0.513). And all haplotypes in Hokkaido were derived from Honshu. This haplotype distribution suggested that the northern Honshu could have served as refuge in Japan. Nested clade analysis (NCA) indicated multiple forces including the vicariance and long-distance dispersal affected the disjunctive distribution among populations of L. hodgsonii in Japan.2. The phylogeography of L. tongolensis，Ligularia tongolensis was distributed along the Jinshajiang watershed, Yalongjiang watershed and Wumeng Mountain. In order to deduce the demographic history of this species, we sequenced two chloroplast DNA (cpDNA) intergenic spacers (trnQ-5’rps16, trnL-rpl32) in 140 individuals from 14 populations of three groups (Jinshajiang vs. Yalongjiang vs. Wumeng) within this species range. High levels of haplotype diversity (Hd = 0.814) and total genetic diversity (HT = 0.862) were detected at the species level, based on a total oftwelve haplotypes identified. However, the intrapopulation diversity (HS = 0.349) was low, which led to the high levels of genetic divergence (GST = 0.595, NST = 0.614, FST = 0.597). In consideration of the speciation of L. tongolensis resulting from the uplifts of the Qinghai-Tibetan Plateau (QTP), we thought the present genetic structure of L. tongolensis was shaped by the fragmentation of ancestral populations during the courses of QTP uplifts. This was further supported by the absence of IBD tests (r = –0.291, p = 0.964), which suggest that the differentiation had not occurred in accordance with the isolation by distance model. The genetic differentiation in L. tongolensis appears to be associated with historical events. Meanwhile, H2 and H5, the dominant haplotypes that located on internal nodes and deviated from extinct ancestral haplotype in the network, were detected to be shared between Jinshajiang and Yalongjiang groups. We deduced that ancestral populations of this species might have had a continuous distribution range, which was then fragmented and isolated by the following tectonic events. Finally, the ancestral polymorphism, H2 and H5, were randomly allocated in Jinshajiang watershed and Yalongjiang watershed. Meanwhile, H5 was the dominant haplotype in Jinshajiang watershed; H7 was the domiant haplotype in Yalongjiang watershed and Wumeng Mountain. This haplotype distribution pattern indicated that each group might have served as a refuge for L. tongolensis during the Quaternary Glaciation. Postglacial demographic expansion was supported by unimodal mismatch distribution and star-like phylogenies, with expansion ages of 274 ka B. P. for this 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a town of Shangri-la County, Diqing Prefecture, was chosen as the main field site for studying the structure and characters of traditional agricultural ecosystem, by using approaches of ethnobotany, cultural anthropology and ecology. Combined with interviewing exercises in Hanpi village, Jiantang Township, this paper also discussed the impact of traditional management on the biocultural diversity. The results showed: Traditional agroecosystem in Shangri-la is an integrated system with three subsystems, which are farming, forest and grazing subsystem. The seasonal shifting grazing activity in Shangri-la, following the natural season change and the recover process of plants, is a sustainable management that protects the local biodiversity. However, along with the decay of shifting grazing tradition recently, the local Tibetans turned to use grass land and forest which is close to villages as the main grazing lands. It increased the pasturing pressure to these areas and caused productivity decreasing and biodiversity. As a symbolic part of Tibetan culture in Shangri-la, the sacred mountain culture has played a significant role in biodiversity conservation by restricting human’s behavior. The Tibetan traditional culture, indigenous knowledge and traditional ecosystem management in Shangri-la has contributed to the biodiversity conservation in this area. However, this research indicated that under the pressure of mainstream culture and market economy, traditional knowledge is vanishing; old crop land races are decreasing; diverse land use management is inclining to be single and seasonal shifting grazing tradition is fading away. The change of diversity to singularity might cause some negative impacts on the local environment and ecosystem. In this paper, advices were also given on how to combine Tibetan traditional knowledge and management experiences into sustainable development of modern agriculture. In this thesis, genetic diversity of Musella lasiocarpa (Franch.) C. Y. Wu ex H. W. Li, a plant endemic to southwest China, was also discussed through the approach of SSR markers. The wild populations of M. lasiocarpa are very rare now due to the habitat fragment and long time human’s disturbance. By conducting broad field investigation, we have found 5 wild populations near the boarder of Yunnan and Sichuan province. Seventeen microsatellite markers were isolated from M. lasiocarpa by using FIASCO method. 8 primers were selected to do the further genetic population structure and genetic diversity analysis. The results showed that genetic diversity of M. lasiocarpa’s wild populations is higher than cultivated populations. The genetic diversity difference between wild and cultivated populations is related to the different reproduction systems. Adopting the way of asexuality reproduction, the genetic basis of cultivated populations become narrow that decrease the genetic diversity. AMOVA analysis showed that 37.19% genetic differentiation is among populations and 62.81% is within population. Genetic differentiation among different populations is related to the limited gene communication. POPGENE analysis indicated that there is very little gene flow among different populations (0.4916), which is the main reason of high genetic differentiation among M. lasiocarpa 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Chemical Studies of Cremanthodium (Asteraceae) Species; Sesquiterpenoids and Related Compounds
期刊论文
NATURAL PRODUCT COMMUNICATIONS, 2019, 卷号: 14, 期号: 9, 页码: 9
Authors:
Tori, Motoo
;
Saito, Yoshinori
;
Gong, Xun
;
Kuroda, Chiaki
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Submit date：2019/12/20
Asteraceae
Cremanthodium
sesquiterpenoid
eremophilane
bisabolane
bakkane
oplopane
eudesmane
Eremophilane, bakkane, secoeremophilane, and secobakkane sesquiterpenoids from Ligularia virgaurea collected in China
期刊论文
TETRAHEDRON, 2019, 卷号: 75, 期号: 14, 页码: 2239-2245
Authors:
Saito, Yoshinori
;
Iga, Saori
;
Hoshiyama, Kie
;
Nakashima, Katsuyuki
;
Okamoto, Yasuko
;
Kuroda, Chiaki
;
Gong, Xun
;
Tori, Motoo
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Submit date：2019/05/24
Ligularia virgaurea
Asteraceae
Eremophilane
Bakkane
Sesquiterpene
Diversity
Molecular evidence for natural hybridization between Ligularia nelumbifolia and Cremanthodium stenoglossum (Asteraceae, Senecioneae)
期刊论文
BOTANY, 2019, 卷号: 97, 期号: 1, 页码: 53-69
Authors:
Ning, Huai
;
Pan, Yue-Zhi
;
Gong, Xun
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Submit date：2019/03/18
natural hybridization
Ligularia
Cremanthodium
cpDNA
ITS
中国植物学会八十五周年学术年会论文集摘要汇编
会议录
Editors:
植物学会
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Submit date：2018/10/24
Maintenance of species boundaries in three sympatric Ligularia (Senecioneae, Asteraceae) species
期刊论文
JOURNAL OF INTEGRATIVE PLANT BIOLOGY, 2018, 卷号: 60, 期号: 10, 页码: 986-999
Authors:
Zhang, Ningning
;
Ma, Yongpeng
;
Folk, Ryan A.
;
Yu, Jiaojun
;
Pan, Yuezhi
;
Gong, Xun
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Submit date：2018/11/12
Biodiversity explains maximum variation in productivity under experimental warming, nitrogen addition, and grazing in mountain grasslands
期刊论文
ECOLOGY AND EVOLUTION, 2018, 卷号: 8, 期号: 20, 页码: 10094-10112
Authors:
Liu, Jiajia
;
Liu, Detuan
;
Xu, Kun
;
Gao, Lian-ming
;
Ge, Xue-jun
;
Burgess, Kevin S.
;
Cadotte, Marc W.
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View/Download：53/8
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Submit date：2018/12/18
Experimental Warming
Functional Diversity
Grazing
Multimodel Inference
Nitrogen Addition
Phylogenetic Diversity
Population genetic dynamics of Himalayan-Hengduan tree peonies, Paeonia subsect. Delavayanae
期刊论文
MOLECULAR PHYLOGENETICS AND EVOLUTION, 2018, 卷号: 125, 期号: 1, 页码: 62-77
Authors:
Zhang, Jin-Mei
;
Lopez-Pujol, Jordi
;
Gong, Xun
;
Wang, Hua-Feng
;
Vilatersana, Roser
;
Zhou, Shi-Liang
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Submit date：2018/07/16
Biogeography
Conservation
Genetic Diversity
Paeonia
Differentiation
无权访问的条目
演示报告
Authors:
钟国跃
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Submit date：2019/05/24
12th全国天然有机化学学术会议摘要集
会议录
Editors:
中国化学会
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Submit date：2018/10/24
Eremophilanes from Ligularia hookeri Collected in China and Structural Revision of 3 beta-Acyloxyfuranoeremophilan-15,6-olide
期刊论文
CHEMICAL & PHARMACEUTICAL BULLETIN, 2018, 卷号: 66, 期号: 6, 页码: 668-673
Authors:
Saito, Yoshinori
;
Shiosaki, Yuko
;
Fujiwara, Miho
;
Mihara, Kanako
;
Nakamizo, Hiromi
;
Otose, Kanako
;
Okamoto, Yasuko
;
Nakashima, Katsuyuki
;
Hanai, Ryo
;
Kuroda, Chiaki
;
Gong, Xun
;
Matsuo, Yosuke
;
Tanaka, Takashi
;
Tori, Motoo
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View/Download：124/11
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Submit date：2018/07/16
Ligularia Hookeri
Asteraceae
Eremophilane
Sesquiterpene
Structure Elucidation
Internal Transcribed Spacer
