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中国科学院昆明植物研究所知识管理系统
Knowledge Management System of Kunming Institute of Botany,CAS
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中国科学院东亚植物... [54]
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王立松 [8]
杨祝良 [3]
Zhang YanY... [3]
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云南植物研究 [52]
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GST, P < 0.05) were exhibited by this species. The SAMOVA revealed seven diverging groups of related chlorotypes, six of them had distinct nonoverlapping geographical ranges: one in the northeast comprising 10 populations, a second with a southeast distribution comprising 22 populations, and the remaning four groups comprising 15 populations located in the west part of the species’ range along different river valleys. The genetic clustering of populations into three regions was also supported by analysis of molecular variance, which showed that most genetic variation (82.43%) was found among these three regions. Two clusters were distinguished by both phylogenetic analysis and genealogical analysis of chlorotypes, one consisting of chlorotypes from the western region and the second consisting of those from the eastern region. Significant genetic differences between the two regions might be attributed to vicariance and restricted gene flow, and this vicariance could be explained by the physical environmental heterogeneity on each side of the Tanaka-Kaiyong Line. Following the uplift of the Tibetan Plateau, the reorganization of the major river drainages was primarily caused by river separation and capture events. These historical events could change the distribution of S. davidii from fragmented to continuous (Upper/Lower Jinshajiang and Yalongjiang/Daduhe), and from continuous to fragmented (Nujiang and Jinshajiang/Honghe). However, spatial and temporal patterns of phylogeographic divergence are strongly associated with historical disjunction rather than modern drainage connections. Moreover, the following north-south split in the eastern region and effective isolation with their genetic diversity were essentially modelled by genetic drift. The higher chlorotype richness and genetic divergence for populations in western region compared with other two regions suggests that there were multipe refugia or in situ survival of S. davidii in the Himalayan-Hengduan Mountain region. Fixation of chlorotypes in the northeastern region and near fixation in the southeastern region suggest a recent colonization of these areas. We further found that this species underwent past range expansion around 37-303 thousand years ago (kya). The southeastern populations likely experienced a demographic expansion via unidirectional gene flow along rivers, while northeastern populations underwent a more northward expansion, both from initial populations (s) (21, 22, 23) preserved on eastern refugia (Jinshajiang). This process might have been accompanied with a series of founder effects or bottlenecks making populations genetically impoverished. 3. Phylogeographic analysisbased on nuclear sequence，We sequenced the nuclear (ncpGS) region in all populations sampled, recovering 23 nuclear haplotypes. Compared to cpDNA, both NST (0.470) and GST (0.338) were relatively lower, but NST was also significantly larger than GST. 37.10% of the total variation was distributed among regions which was much lower than that shown by chlorotypes. Thus, more extensive distribution of nuclear haplotypes was exhibited across the geographical range instead of the strong population subdivision observed in chlorotypes. Similarly to the chloroplast data, we found that genetic differentiation of nDNA was positively correlated with the geographical distance, but the increase in the geographical distance between populations did not increase the genetic differentiation of nDNA as rapidly as that of cpDNA. These contrasting levels between the chloroplast and nuclear genomes of S. davidii are likely due to limited gene flow of cpDNA by seeds vs. the extensive gene flow of nDNA by wind-mediated pollen in the population history. We also determined from nuclear markers that haplotype diversity was reduced in the southeastern and northeastern regions due to the loss of rare haplotypes in western region. This reduction of gene diversity is also a signature of founder events or recent bottleneck during post-glacial colonization. However, nuclear diversity within populations remains high. This provides evidence that regionally pollen flow might be sufficiently high to blur the genetic identity of founder populations over a reasonably large spatial scale.3. Relationships among three varieties，The phylogenetic analysis identified two phylogroups of chlorotypes, corresponding to S. davidii var. davidii and var. chuansinesis. The former was distinguished by the abscence of predonminant nuclear haplotype H1 of the latter. The monophyletic group of chlorotypes in var. davidii and var. liangshanesis showed their relatively close relationship. And their genetic divergence from the third variety appears to be relative to their slight morphological difference in leaf size and the divergent environmental niche spaces they occupy. Thus, the observed differences in morphological characters between var. chuansinesis and other two varieties can be explained by the seed dispersal limitation illustrated above (as inferred by geographical separation) and by environmental heterogeneity (as inferred by precipitation or elevation) or by a combination of both. After all, the geological changes, drainage reorganization, and floristic differences following the Himalayan uplift have been suggested to affect the genetic structure of S. davidii. These results provide new insights into the phylogeographic pattern of plants in China. In addition, the unique population genetic structure found in S. davidii has provided important insights into the evolutionary history of this species. The genetic profile uncovered in this study is also critical for its conservation management. Our study has uncovered the existence of at least two ‘evolutionary significant units’ independent units within S. davidii, corresponding to var. davidii from eastern region and var. chuansinensis from western region. The conservation efforts should first focus on most western populations and on the southeastern ones exhibiting high levels of genetic diversity, while the genetically homogeneous northeastern populations located in the degraded Loess Plateau should require much greater conservation 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dissertation is composed of three chapters. Chapter 1 provided an introduction to the investigation on the chemical constituents of two species of fern, including L. japonicium and S. doederleinii. In the second chapter, we described the isolation and identification of chemical constituents from three species of Viburnum, including V. odoratissimum, V. odoratissimum var. sessilifolrum, and V. foetidum. var. ceanothoides. In the last chapter, we summarized all the natural vibsane-type diterpenoids, and the activity and the synthesis research about them. By means of chromatography methods and spectroscopic evidences, 188 compounds were isolated and identified from the five species of plants, among which 43 compounds were new. The classes of these compounds include Lycopodium alkaloid, triterpenoid, diterpenoid, vibsane-type diterpenoid, steroid, lignan, flavnone, coumarin, phenol, phenolic glycoside. A Lycopodium alkaloid with a novel skeleton, lycojapodine A, was isolated from L. japonicium, as well as other 14 new ones. And 16 vibsane-type diterpenoid were isolated from species of Viburnum, 10 of which were new ones.All the new Lycopodium alkaloid isolated from L. japonicium were evaluated for the inhibition activity of acetylcholine esterase (AChE), part of the known Lycopodium alkaloid isolated from L. japonicium were evaluated for the anticancer activity, and most of the known Lycopodium alkaloid isolated from L. japonicium were evaluated for anti-HIV activity. Some of the vibsane-type diterpenoids were tested for the activity of inducing differentiation about leukaemia, controlling the migration of macrophages, and inducing morphological change in NGF-mediated PC-12 cell.","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&sort_by=2&isNonaffiliated=false&search_type=-1&query1=%25E5%259C%25B0%25E8%25A1%25A3&order=desc&&fq=dc.project.title_filter%3AThe%5C+dissertation%5C+is%5C+composed%5C+of%5C+three%5C+chapters.%5C+Chapter%5C+1%5C+provided%5C+an%5C+introduction%5C+to%5C+the%5C+investigation%5C+on%5C+the%5C+chemical%5C+constituents%5C+of%5C+two%5C+species%5C+of%5C+fern%2C%5C+including%5C+L.%5C+japonicium%5C+and%5C+S.%5C+doederleinii.%5C+In%5C+the%5C+second%5C+chapter%2C%5C+we%5C+described%5C+the%5C+isolation%5C+and%5C+identification%5C+of%5C+chemical%5C+constituents%5C+from%5C+three%5C+species%5C+of%5C+Viburnum%2C%5C+including%5C+V.%5C+odoratissimum%2C%5C+V.%5C+odoratissimum%5C+var.%5C+sessilifolrum%2C%5C+and%5C+V.%5C+foetidum.%5C+var.%5C+ceanothoides.%5C+In%5C+the%5C+last%5C+chapter%2C%5C+we%5C+summarized%5C+all%5C+the%5C+natural%5C+vibsane%5C-type%5C+diterpenoids%2C%5C+and%5C+the%5C+activity%5C+and%5C+the%5C+synthesis%5C+research%5C+about%5C+them.%5C+By%5C+means%5C+of%5C+chromatography%5C+methods%5C+and%5C+spectroscopic%5C+evidences%2C%5C+188%5C+compounds%5C+were%5C+isolated%5C+and%5C+identified%5C+from%5C+the%5C+five%5C+species%5C+of%5C+plants%2C%5C+among%5C+which%5C+43%5C+compounds%5C+were%5C+new.%5C+The%5C+classes%5C+of%5C+these%5C+compounds%5C+include%5C+Lycopodium%5C+alkaloid%2C%5C+triterpenoid%2C%5C+diterpenoid%2C%5C+vibsane%5C-type%5C+diterpenoid%2C%5C+steroid%2C%5C+lignan%2C%5C+flavnone%2C%5C+coumarin%2C%5C+phenol%2C%5C+phenolic%5C+glycoside.%5C+A%5C+Lycopodium%5C+alkaloid%5C+with%5C+a%5C+novel%5C+skeleton%2C%5C+lycojapodine%5C+A%2C%5C+was%5C+isolated%5C+from%5C+L.%5C+japonicium%2C%5C+as%5C+well%5C+as%5C+other%5C+14%5C+new%5C+ones.%5C+And%5C+16%5C+vibsane%5C-type%5C+diterpenoid%5C+were%5C+isolated%5C+from%5C+species%5C+of%5C+Viburnum%2C%5C+10%5C+of%5C+which%5C+were%5C+new%5C+ones.All%5C+the%5C+new%5C+Lycopodium%5C+alkaloid%5C+isolated%5C+from%5C+L.%5C+japonicium%5C+were%5C+evaluated%5C+for%5C+the%5C+inhibition%5C+activity%5C+of%5C+acetylcholine%5C+esterase%5C+%5C%28AChE%5C%29%2C%5C+part%5C+of%5C+the%5C+known%5C+Lycopodium%5C+alkaloid%5C+isolated%5C+from%5C+L.%5C+japonicium%5C+were%5C+evaluated%5C+for%5C+the%5C+anticancer%5C+activity%2C%5C+and%5C+most%5C+of%5C+the%5C+known%5C+Lycopodium%5C+alkaloid%5C+isolated%5C+from%5C+L.%5C+japonicium%5C+were%5C+evaluated%5C+for%5C+anti%5C-HIV%5C+activity.%5C+Some%5C+of%5C+the%5C+vibsane%5C-type%5C+diterpenoids%5C+were%5C+tested%5C+for%5C+the%5C+activity%5C+of%5C+inducing%5C+differentiation%5C+about%5C+leukaemia%2C%5C+controlling%5C+the%5C+migration%5C+of%5C+macrophages%2C%5C+and%5C+inducing%5C+morphological%5C+change%5C+in%5C+NGF%5C-mediated%5C+PC%5C-12%5C+cell."},{"jsname":"This dissertation is composed of five chapters, in which the chemical constituents of four medicinal plants, Hypoxis aurea Lour., Albizia mollis (Wall.) Boiv., Dobinea delavayi (Baill.) Baill. and Poria cococs (Schw.) Wolf, which are belonged to families of Hypoxidaceae, Leguminosae, Polyporaceae and Podoaceae, respectively, have been phytochemically studied. Chapter 5 reviews the chemical and bioactivities progresses of Albizia and Hypoxis genera. 79 compounds, including 12 new ones, were isolated from the above mentioned four species. All of the components were separated by column chromatography (siliga gel, sephadex LH-20, RP-18, RP-8 and half-preparation HPLC) and identified on the basis of extensive spectroscopic methods. The type of all compounds include phenolic glycosides, triterpenoids (lanosterol, oleanane type), diterpenoids (ent-kaurane type), eudesmane sesquiterpenes, lignanoids, ceramides, flavones, fatty acid (ester). We put forward a proposal that Albizia and Hypoxis genera should belong to Hypoxidaceae and be independent from Amaryllidaceae. Parts of compounds have been or being texted for antineoplastic, antidepressant.","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&sort_by=2&isNonaffiliated=false&search_type=-1&query1=%25E5%259C%25B0%25E8%25A1%25A3&order=desc&&fq=dc.project.title_filter%3AThis%5C+dissertation%5C+is%5C+composed%5C+of%5C+five%5C+chapters%2C%5C+in%5C+which%5C+the%5C+chemical%5C+constituents%5C+of%5C+four%5C+medicinal%5C+plants%2C%5C+Hypoxis%5C+aurea%5C+Lour.%2C%5C+Albizia%5C+mollis%5C+%5C%28Wall.%5C%29%5C+Boiv.%2C%5C+Dobinea%5C+delavayi%5C+%5C%28Baill.%5C%29%5C+Baill.%5C+and%5C+Poria%5C+cococs%5C+%5C%28Schw.%5C%29%5C+Wolf%2C%5C+which%5C+are%5C+belonged%5C+to%5C+families%5C+of%5C+Hypoxidaceae%2C%5C+Leguminosae%2C%5C+Polyporaceae%5C+and%5C+Podoaceae%2C%5C+respectively%2C%5C+have%5C+been%5C+phytochemically%5C+studied.%5C+Chapter%5C+5%5C+reviews%5C+the%5C+chemical%5C+and%5C+bioactivities%5C+progresses%5C+of%5C+Albizia%5C+and%5C+Hypoxis%5C+genera.%5C+79%5C+compounds%2C%5C+including%5C+12%5C+new%5C+ones%2C%5C+were%5C+isolated%5C+from%5C+the%5C+above%5C+mentioned%5C+four%5C+species.%5C+All%5C+of%5C+the%5C+components%5C+were%5C+separated%5C+by%5C+column%5C+chromatography%5C+%5C%28siliga%5C+gel%2C%5C+sephadex%5C+LH%5C-20%2C%5C+RP%5C-18%2C%5C+RP%5C-8%5C+and%5C+half%5C-preparation%5C+HPLC%5C%29%5C+and%5C+identified%5C+on%5C+the%5C+basis%5C+of%5C+extensive%5C+spectroscopic%5C+methods.%5C+The%5C+type%5C+of%5C+all%5C+compounds%5C+include%5C+phenolic%5C+glycosides%2C%5C+triterpenoids%5C+%5C%28lanosterol%2C%5C+oleanane%5C+type%5C%29%2C%5C+diterpenoids%5C+%5C%28ent%5C-kaurane%5C+type%5C%29%2C%5C+eudesmane%5C+sesquiterpenes%2C%5C+lignanoids%2C%5C+ceramides%2C%5C+flavones%2C%5C+fatty%5C+acid%5C+%5C%28ester%5C%29.%5C+We%5C+put%5C+forward%5C+a%5C+proposal%5C+that%5C+Albizia%5C+and%5C+Hypoxis%5C+genera%5C+should%5C+belong%5C+to%5C+Hypoxidaceae%5C+and%5C+be%5C+independent%5C+from%5C+Amaryllidaceae.%5C+Parts%5C+of%5C+compounds%5C+have%5C+been%5C+or%5C+being%5C+texted%5C+for%5C+antineoplastic%2C%5C+antidepressant."},{"jsname":"lastIndexed","jscount":"2021-01-21"}],"Funding Project","dc.project.title_filter")'>
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西双版纳白茫雪山波罗的海南岸地衣纯培养放线菌多样性
期刊论文
微生物学通报, 2019, 卷号: 46, 期号: 9, 页码: 2157-2165
Authors:
姜龙芊
;
张坤
;
李桂鼎
;
王欣宇
;
姜怡
;
王立松
;
姜成林
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Submit date：2019/12/20
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梁萌萌
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Submit date：2019/03/18
中国西南山地关键高山草本植物的分布区变迁与预测
学位论文
博士, 2018
Authors:
何谐
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Submit date：2021/01/05
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影音
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kib
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中国植物学会八十五周年学术年会论文集摘要汇编
会议录
Editors:
植物学会
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Submit date：2018/10/24
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演示报告
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刘冬梅
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林业规划中植物考察资料总结和分析：以新平磨盘山为例
学位论文
硕士, 2018
Authors:
李园园
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米团花萜类合酶功能鉴定和二倍半萜生物活性探索
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硕士, 2018
Authors:
凌伊
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火把花萜类合酶基因克隆、功能鉴定及其工程菌株的构建
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硕士, 2018
Authors:
李德森
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专著
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kib
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