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中国科学院昆明植物研究所知识管理系统
Knowledge Management System of Kunming Institute of Botany,CAS
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0.05) between wild (AR = 4.651), semi-cultivated (AR = 5.091) and cultivated (AR = 5.132) populations of C. taliensis, which suggested that the genetic background of long-lived woody plant was not easy to be changed, and there were moderate high gene flow between populations. However, there was a significant difference (P < 0.05) between wild (AR = 5.9) and cultivated (AR = 7.1) populations distributed in the same place in Yun county, Yunnan province, which may result from the hybridization and introgression of species in the tea garden and anthropogenic damages to the wild population. The hypothesis of hybrid origin of C. grandibracteata was tested by morphological and microsatellites analyses. Compared with other species, the locules in ovary of C. grandibracteata are variable, which showed a morphological intermediate and mosaic. Except one private allele, Ninety-nine percent alleles of C. grandibracteata were shared with these of C. taliensis and C. sinensis var. assamica. And C. grandibracteata was nested in the cluster of C. taliensis in the UPGMA tree. Conclusively, our results supported the hypothesis of hybrid origin of C. grandibracteata partly. The speciation of C. grandibracteata was derived from hybridization and asymmetrical introgression potentially. It is possible that C. taliensis was one of its parents, but it still needs more evidences to prove that C. sinensis var. assamica was another parent.","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&sort_by=2&isNonaffiliated=false&search_type=-1&query1=POLYMERASE-CHAIN-REACTION&order=desc&&fq=dc.project.title_filter%3ACamellia%5C+taliensis%5C+%5C%28W.%5C+W.%5C+Smith%5C%29%5C+Melchior%2C%5C+a%5C+member%5C+of%5C+Camellia%5C+sect.%5C+Thea%2C%5C+is%5C+an%5C+indigenous%5C+species%5C+in%5C+local%5C+natural%5C+forest%5C+and%5C+has%5C+a%5C+long%5C+cultivative%5C+history%5C+in%5C+western%5C+Yunnan%5C+and%5C+its%5C+neighborhood%2C%5C+where%5C+the%5C+domestications%5C+of%5C+this%5C+species%5C+in%5C+different%5C+historical%5C+periods%5C+and%5C+in%5C+different%5C+ways%5C+can%5C+be%5C+found.%5C+C.%5C+taliensis%5C+is%5C+an%5C+important%5C+contributor%5C+to%5C+the%5C+formations%5C+of%5C+tea%5C+landraces%5C+by%5C+hybridization%5C+and%5C+introgression.%5C+In%5C+the%5C+present%5C+study%2C%5C+14%5C+microsatellite%5C+loci%5C+screened%5C+from%5C+37%5C+loci%5C+were%5C+used%5C+to%5C+explore%5C+the%5C+genetic%5C+diversity%5C+about%5C+this%5C+species%5C+with%5C+579%5C+samples%5C+from%5C+25%5C+populations%5C+%5C%2816%5C+wild%5C+populations%2C%5C+4%5C+semi%5C-cultivated%5C+populations%5C+and%5C+5%5C+cultivated%5C+populations%5C%29.%5C+At%5C+the%5C+same%5C+time%2C%5C+the%5C+potential%5C+hybrid%5C+speciation%5C+of%5C+C.%5C+grandibracteata%2C%5C+was%5C+investigated%5C+using%5C+39%5C+individuals%5C+from%5C+2%5C+populations%2C%5C+along%5C+with%5C+C.%5C+taliensis%5C+and%5C+C.%5C+sinensis%5C+var.%5C+assamica%5C+%5C%2883%5C+individuals%5C+from%5C+4%5C+populations%5C%29%5C+by%5C+the%5C+same%5C+microsatellite%5C+markers.%5C+C.%5C+taliensis%5C+had%5C+a%5C+moderate%5C+high%5C+level%5C+of%5C+genetic%5C+diversity%5C+%5C%28A%5C+%3D%5C+14.3%2C%5C+Ne%3D%5C+5.7%2C%5C+HE%5C+%3D%5C+0.666%2C%5C+I%5C+%3D%5C+1.753%2C%5C+AR%5C+%3D%5C+7.2%2C%5C+PPB%5C+%3D%5C+100%25%5C%29.%5C+This%5C+may%5C+result%5C+from%5C+several%5C+factors%5C+including%5C+K%5C-strategy%2C%5C+genetic%5C+background%2C%5C+gene%5C+flow%5C+between%5C+populations%2C%5C+hybridization%5C+and%5C+introgression%5C+among%5C+species.%5C+Between%5C+wild%5C+populations%5C+of%5C+C.%5C+taliensis%2C%5C+the%5C+gene%5C+flow%5C+was%5C+moderate%5C+high%5C+%5C%28Nm%5C+%3D%5C+1.197%5C%29%2C%5C+and%5C+genetic%5C+variation%5C+was%5C+less%5C+than%5C+20%25%5C+%5C%28GST%5C+%3D%5C+0.147%2C%5C+FST%5C+%3D%5C+0.173%5C%29%2C%5C+which%5C+was%5C+similar%5C+to%5C+other%5C+research%5C+results%5C+of%5C+long%5C-lived%5C+woody%5C+plants%2C%5C+and%5C+reflected%5C+the%5C+genetic%5C+structure%5C+of%5C+its%5C+ancestry%5C+to%5C+same%5C+extent.%5C+There%5C+was%5C+a%5C+high%5C+significant%5C+correlation%5C+between%5C+geographic%5C+distance%5C+and%5C+Nei%E2%80%99s%5C+genetic%5C+distance%5C+%5C%28r%5C+%3D%5C+0.372%2C%5C+P%5C+%3D%5C+0.001%5C%29%5C+of%5C+populations%2C%5C+which%5C+accorded%5C+with%5C+isolation%5C+by%5C+distance%5C+model.%5C+Inferring%5C+from%5C+Bayesian%5C+clustering%5C+of%5C+genotypes%2C%5C+all%5C+individuals%5C+of%5C+C.%5C+taliensis%5C+were%5C+divided%5C+into%5C+two%5C+groups%2C%5C+conflicting%5C+with%5C+the%5C+result%5C+based%5C+on%5C+Nei%E2%80%99s%5C+genetic%5C+distance%5C+and%5C+real%5C+geographic%5C+distribution%2C%5C+which%5C+suggested%5C+there%5C+were%5C+heavy%5C+and%5C+non%5C-random%5C+influences%5C+by%5C+human%5C+practices.%5C+According%5C+to%5C+allelic%5C+richness%2C%5C+there%5C+were%5C+no%5C+significant%5C+differences%5C+%5C%28P%5C+%3E%5C+0.05%5C%29%5C+between%5C+wild%5C+%5C%28AR%5C+%3D%5C+4.651%5C%29%2C%5C+semi%5C-cultivated%5C+%5C%28AR%5C+%3D%5C+5.091%5C%29%5C+and%5C+cultivated%5C+%5C%28AR%5C+%3D%5C+5.132%5C%29%5C+populations%5C+of%5C+C.%5C+taliensis%2C%5C+which%5C+suggested%5C+that%5C+the%5C+genetic%5C+background%5C+of%5C+long%5C-lived%5C+woody%5C+plant%5C+was%5C+not%5C+easy%5C+to%5C+be%5C+changed%2C%5C+and%5C+there%5C+were%5C+moderate%5C+high%5C+gene%5C+flow%5C+between%5C+populations.%5C+However%2C%5C+there%5C+was%5C+a%5C+significant%5C+difference%5C+%5C%28P%5C+%3C%5C+0.05%5C%29%5C+between%5C+wild%5C+%5C%28AR%5C+%3D%5C+5.9%5C%29%5C+and%5C+cultivated%5C+%5C%28AR%5C+%3D%5C+7.1%5C%29%5C+populations%5C+distributed%5C+in%5C+the%5C+same%5C+place%5C+in%5C+Yun%5C+county%2C%5C+Yunnan%5C+province%2C%5C+which%5C+may%5C+result%5C+from%5C+the%5C+hybridization%5C+and%5C+introgression%5C+of%5C+species%5C+in%5C+the%5C+tea%5C+garden%5C+and%5C+anthropogenic%5C+damages%5C+to%5C+the%5C+wild%5C+population.%5C+The%5C+hypothesis%5C+of%5C+hybrid%5C+origin%5C+of%5C+C.%5C+grandibracteata%5C+was%5C+tested%5C+by%5C+morphological%5C+and%5C+microsatellites%5C+analyses.%5C+Compared%5C+with%5C+other%5C+species%2C%5C+the%5C+locules%5C+in%5C+ovary%5C+of%5C+C.%5C+grandibracteata%5C+are%5C+variable%2C%5C+which%5C+showed%5C+a%5C+morphological%5C+intermediate%5C+and%5C+mosaic.%5C+Except%5C+one%5C+private%5C+allele%2C%5C+Ninety%5C-nine%5C+percent%5C+alleles%5C+of%5C+C.%5C+grandibracteata%5C+were%5C+shared%5C+with%5C+these%5C+of%5C+C.%5C+taliensis%5C+and%5C+C.%5C+sinensis%5C+var.%5C+assamica.%5C+And%5C+C.%5C+grandibracteata%5C+was%5C+nested%5C+in%5C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Scholarship Council","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&sort_by=2&isNonaffiliated=false&search_type=-1&query1=POLYMERASE-CHAIN-REACTION&order=desc&&fq=dc.project.title_filter%3AChina%5C+Scholarship%5C+Council"},{"jsname":"Chinese Academy of Sciences[2013T2S003]","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&sort_by=2&isNonaffiliated=false&search_type=-1&query1=POLYMERASE-CHAIN-REACTION&order=desc&&fq=dc.project.title_filter%3AChinese%5C+Academy%5C+of%5C+Sciences%5C%5B2013T2S003%5C%5D"},{"jsname":"Cold stress is one of the major environmental factors that adversely influence plants growth. Cold stress not only limits plants geographic distribution, but also reduces plants yield by shortening growing season, which brought billions of dollars economic losses for global crop. In nature, responses of overwintering plants to low temperature can be divided into three distinct phases: cold acclimation (CA), freezing, and post-freezing recovery (PFR). Until now, plenty intensive study about molecular mechanism of cold stress mainly focused on the above-zero low temperature phase. However, the studies on the freezing phase below zero and the following PFR phase with temperature going up to above-zero were rare. The previous research form our lab hinted that the responses of plants to freezing and PFR were complex and important. Except for passive reflection, there were also crucial active responses during this process. Several special rules were presented at the different levels including gene expression, signal transduction and membrane lipids changes, and fully understanding these rules would be helpful for us to explore the responses of plants to low temperature and then proceed to improve the freezing resistance of plants. In the present study, the mechanisms of respond to freezing and PFR of model plant Arabidopsis thaliana and its close relative Thellungiella halophlia that with extreme tolerance to abiotic stresses were carried out, including regulation of gene expression, signal transduction pathway and membrane lipids changes three levels which were essential for the freezing resistance of plants. Ground on these work, we obtained results from the following five aspects. First, the complete picture of A. thaliana responding to freezing and PFR at transcriptome level was elaborated and three functional genes closely related to the phases were identified. Second, the cis-elements with high frequent presence in differentially expressed genes were elucidated, and the practical binding of one elements among them was experimental verified during freezing and PFR. Moreover, we predicted the new elements which would respond to freezing and PFR. Third, the regulation of freezing stress by microRNA in A. thaliana was preliminarily investigated and 36 functional genes possibly regulated by miRNA during freezing and PFR were gained. Fourth, the negative effect of phytohormone Auxin on A. thaliana subjected to freezing stress was identified. Fifth, for the freezing-resistant plant T. halophlia, the rules of membrane lipids composition changes under freezing stress were uncovered.","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&sort_by=2&isNonaffiliated=false&search_type=-1&query1=POLYMERASE-CHAIN-REACTION&order=desc&&fq=dc.project.title_filter%3ACold%5C+stress%5C+is%5C+one%5C+of%5C+the%5C+major%5C+environmental%5C+factors%5C+that%5C+adversely%5C+influence%5C+plants%5C+growth.%5C+Cold%5C+stress%5C+not%5C+only%5C+limits%5C+plants%5C+geographic%5C+distribution%2C%5C+but%5C+also%5C+reduces%5C+plants%5C+yield%5C+by%5C+shortening%5C+growing%5C+season%2C%5C+which%5C+brought%5C+billions%5C+of%5C+dollars%5C+economic%5C+losses%5C+for%5C+global%5C+crop.%5C+In%5C+nature%2C%5C+responses%5C+of%5C+overwintering%5C+plants%5C+to%5C+low%5C+temperature%5C+can%5C+be%5C+divided%5C+into%5C+three%5C+distinct%5C+phases%5C%3A%5C+cold%5C+acclimation%5C+%5C%28CA%5C%29%2C%5C+freezing%2C%5C+and%5C+post%5C-freezing%5C+recovery%5C+%5C%28PFR%5C%29.%5C+Until%5C+now%2C%5C+plenty%5C+intensive%5C+study%5C+about%5C+molecular%5C+mechanism%5C+of%5C+cold%5C+stress%5C+mainly%5C+focused%5C+on%5C+the%5C+above%5C-zero%5C+low%5C+temperature%5C+phase.%5C+However%2C%5C+the%5C+studies%5C+on%5C+the%5C+freezing%5C+phase%5C+below%5C+zero%5C+and%5C+the%5C+following%5C+PFR%5C+phase%5C+with%5C+temperature%5C+going%5C+up%5C+to%5C+above%5C-zero%5C+were%5C+rare.%5C+The%5C+previous%5C+research%5C+form%5C+our%5C+lab%5C+hinted%5C+that%5C+the%5C+responses%5C+of%5C+plants%5C+to%5C+freezing%5C+and%5C+PFR%5C+were%5C+complex%5C+and%5C+important.%5C+Except%5C+for%5C+passive%5C+reflection%2C%5C+there%5C+were%5C+also%5C+crucial%5C+active%5C+responses%5C+during%5C+this%5C+process.%5C+Several%5C+special%5C+rules%5C+were%5C+presented%5C+at%5C+the%5C+different%5C+levels%5C+including%5C+gene%5C+expression%2C%5C+signal%5C+transduction%5C+and%5C+membrane%5C+lipids%5C+changes%2C%5C+and%5C+fully%5C+understanding%5C+these%5C+rules%5C+would%5C+be%5C+helpful%5C+for%5C+us%5C+to%5C+explore%5C+the%5C+responses%5C+of%5C+plants%5C+to%5C+low%5C+temperature%5C+and%5C+then%5C+proceed%5C+to%5C+improve%5C+the%5C+freezing%5C+resistance%5C+of%5C+plants.%5C+In%5C+the%5C+present%5C+study%2C%5C+the%5C+mechanisms%5C+of%5C+respond%5C+to%5C+freezing%5C+and%5C+PFR%5C+of%5C+model%5C+plant%5C+Arabidopsis%5C+thaliana%5C+and%5C+its%5C+close%5C+relative%5C+Thellungiella%5C+halophlia%5C+that%5C+with%5C+extreme%5C+tolerance%5C+to%5C+abiotic%5C+stresses%5C+were%5C+carried%5C+out%2C%5C+including%5C+regulation%5C+of%5C+gene%5C+expression%2C%5C+signal%5C+transduction%5C+pathway%5C+and%5C+membrane%5C+lipids%5C+changes%5C+three%5C+levels%5C+which%5C+were%5C+essential%5C+for%5C+the%5C+freezing%5C+resistance%5C+of%5C+plants.%5C+Ground%5C+on%5C+these%5C+work%2C%5C+we%5C+obtained%5C+results%5C+from%5C+the%5C+following%5C+five%5C+aspects.%5C+First%2C%5C+the%5C+complete%5C+picture%5C+of%5C+A.%5C+thaliana%5C+responding%5C+to%5C+freezing%5C+and%5C+PFR%5C+at%5C+transcriptome%5C+level%5C+was%5C+elaborated%5C+and%5C+three%5C+functional%5C+genes%5C+closely%5C+related%5C+to%5C+the%5C+phases%5C+were%5C+identified.%5C+Second%2C%5C+the%5C+cis%5C-elements%5C+with%5C+high%5C+frequent%5C+presence%5C+in%5C+differentially%5C+expressed%5C+genes%5C+were%5C+elucidated%2C%5C+and%5C+the%5C+practical%5C+binding%5C+of%5C+one%5C+elements%5C+among%5C+them%5C+was%5C+experimental%5C+verified%5C+during%5C+freezing%5C+and%5C+PFR.%5C+Moreover%2C%5C+we%5C+predicted%5C+the%5C+new%5C+elements%5C+which%5C+would%5C+respond%5C+to%5C+freezing%5C+and%5C+PFR.%5C+Third%2C%5C+the%5C+regulation%5C+of%5C+freezing%5C+stress%5C+by%5C+microRNA%5C+in%5C+A.%5C+thaliana%5C+was%5C+preliminarily%5C+investigated%5C+and%5C+36%5C+functional%5C+genes%5C+possibly%5C+regulated%5C+by%5C+miRNA%5C+during%5C+freezing%5C+and%5C+PFR%5C+were%5C+gained.%5C+Fourth%2C%5C+the%5C+negative%5C+effect%5C+of%5C+phytohormone%5C+Auxin%5C+on%5C+A.%5C+thaliana%5C+subjected%5C+to%5C+freezing%5C+stress%5C+was%5C+identified.%5C+Fifth%2C%5C+for%5C+the%5C+freezing%5C-resistant%5C+plant%5C+T.%5C+halophlia%2C%5C+the%5C+rules%5C+of%5C+membrane%5C+lipids%5C+composition%5C+changes%5C+under%5C+freezing%5C+stress%5C+were%5C+uncovered."},{"jsname":"Construction Program of Biology First-class Discipline in Guizhou[CINYL [2017] 009]","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&sort_by=2&isNonaffiliated=false&search_type=-1&query1=POLYMERASE-CHAIN-REACTION&order=desc&&fq=dc.project.title_filter%3AConstruction%5C+Program%5C+of%5C+Biology%5C+First%5C-class%5C+Discipline%5C+in%5C+Guizhou%5C%5BCINYL%5C+%5C%5B2017%5C%5D%5C+009%5C%5D"},{"jsname":"Czech Science Foundation, GAR[P506/14/13541S]","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&sort_by=2&isNonaffiliated=false&search_type=-1&query1=POLYMERASE-CHAIN-REACTION&order=desc&&fq=dc.project.title_filter%3ACzech%5C+Science%5C+Foundation%2C%5C+GAR%5C%5BP506%5C%2F14%5C%2F13541S%5C%5D"},{"jsname":"During a field trip at a brule in Shangri-La, a mixed population of Ligularia Cass. was found, which including L. subspicata (Bur. et Franch.) Hand.-Mazz., L. nelumbifolia (Bur. et Franch.) Hand.-Mazz., L. tongolensis (Franch.) Hand.-Mazz., L. cymbulifera (W.W.Smith) Hand.-Mazz., L. lingiana S.W.Liu, and also some individuals morphologically intermediate between L. subspicata and L. nelumbifolia. Hence, these intermediate individuals were preliminarily assumed as natural hybrids of the two Ligularia. According to their morphology, they’re assumed to form hybrids A and B. Through careful comparison of specimens in herbarium and those we collected, the inflorescence of putative hybrid A is close to L. nelumbifolia, but the shape of laminae are intergradation of L. subspicata and L. nelumbifolia; overall morphology of putative hybrids B is similar to L. nelumbifolia, but inflorescence color is as same as L. subspicata. Compared to L. nelumbifolia (39%) and L. subspicata (36.8%), the germination rate of putative hybrid B (45.7%) slightly higher than the two; but that of hybrid A is extraordinarily low (0.3%). One possible interpretation of the low rate is hybridization. 60 individuals were collected, including putative parents, other 4 species of Ligularia nearby, putative hybrid A and B. They were all direct sequenced of four cpDNA fragments, and direct sequenced or cloning sequenced of nrDNA ITS4-5. The results support that L. nelumbifolia and L. subspicata are parents of putative hybrid A, and the majority female parent is L. subspicata, L. vellerea may also be involved in the hybridization in some degree; the nuclear sequences of putative hybrid B have no superposition, and its chloroplast DNA sequences are identical with L. nelumbifolia, so putative hybrid B could not be hybrid; and there are backcross individuals exist among the putative parent L. subspicata. NewHybrids analysis of ISSR markers indicated that, the individuals of putative hybrid A are almost L. nelumbifolia and L. subspicata F1 hybrid generation (10/11), only 1/11 possibly backcross or other forms; all individuals of hybrid B are L. nelumbifolia; except one individual of L. subspicata as backcrossed, the other parent individuals are 100% reliable. This study focused on molecular evidence, complemented by ecological, reproductive and other characteristics, we demonstrated that the morphologically intermediate individuals’ origin, and the probability of belonging to each parental or hybrid class. And concluded that L. nelumbifolia and L. subspicata are the parents of putative hybrid A, L. vellerea may also be involved in the hybridization in some degree, hybrids mainly are the first generation, a few individuals may be involved in backcross, and most probably backcross with L. subspicata according to the anthesis, while the assumption of hybrid B is not supported.","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&sort_by=2&isNonaffiliated=false&search_type=-1&query1=POLYMERASE-CHAIN-REACTION&order=desc&&fq=dc.project.title_filter%3ADuring%5C+a%5C+field%5C+trip%5C+at%5C+a%5C+brule%5C+in%5C+Shangri%5C-La%2C%5C+a%5C+mixed%5C+population%5C+of%5C+Ligularia%5C+Cass.%5C+was%5C+found%2C%5C+which%5C+including%5C+L.%5C+subspicata%5C+%5C%28Bur.%5C+et%5C+Franch.%5C%29%5C+Hand.%5C-Mazz.%2C%5C+L.%5C+nelumbifolia%5C+%5C%28Bur.%5C+et%5C+Franch.%5C%29%5C+Hand.%5C-Mazz.%2C%5C+L.%5C+tongolensis%5C+%5C%28Franch.%5C%29%5C+Hand.%5C-Mazz.%2C%5C+L.%5C+cymbulifera%5C+%5C%28W.W.Smith%5C%29%5C+Hand.%5C-Mazz.%2C%5C+L.%5C+lingiana%5C+S.W.Liu%2C%5C+and%5C+also%5C+some%5C+individuals%5C+morphologically%5C+intermediate%5C+between%5C+L.%5C+subspicata%5C+and%5C+L.%5C+nelumbifolia.%5C+Hence%2C%5C+these%5C+intermediate%5C+individuals%5C+were%5C+preliminarily%5C+assumed%5C+as%5C+natural%5C+hybrids%5C+of%5C+the%5C+two%5C+Ligularia.%5C+According%5C+to%5C+their%5C+morphology%2C%5C+they%E2%80%99re%5C+assumed%5C+to%5C+form%5C+hybrids%5C+A%5C+and%5C+B.%5C+Through%5C+careful%5C+comparison%5C+of%5C+specimens%5C+in%5C+herbarium%5C+and%5C+those%5C+we%5C+collected%2C%5C+the%5C+inflorescence%5C+of%5C+putative%5C+hybrid%5C+A%5C+is%5C+close%5C+to%5C+L.%5C+nelumbifolia%2C%5C+but%5C+the%5C+shape%5C+of%5C+laminae%5C+are%5C+intergradation%C2%A0of%5C+L.%5C+subspicata%5C+and%5C+L.%5C+nelumbifolia%5C%3B%5C+overall%5C+morphology%5C+of%5C+putative%5C+hybrids%5C+B%5C+is%5C+similar%5C+to%5C+L.%5C+nelumbifolia%2C%5C+but%5C+inflorescence%5C+color%5C+is%5C+as%5C+same%5C+as%5C+L.%5C+subspicata.%5C+Compared%5C+to%5C+L.%5C+nelumbifolia%5C+%5C%2839%25%5C%29%5C+and%5C+L.%5C+subspicata%5C+%5C%2836.8%25%5C%29%2C%5C+the%5C+germination%5C+rate%5C+of%5C+putative%5C+hybrid%5C+B%5C+%5C%2845.7%25%5C%29%5C+slightly%5C+higher%5C+than%5C+the%5C+two%5C%3B%5C+but%5C+that%5C+of%5C+hybrid%5C+A%5C+is%5C+extraordinarily%5C+low%5C+%5C%280.3%25%5C%29.%5C+One%5C+possible%5C+interpretation%5C+of%5C+the%5C+low%5C+rate%5C+is%5C+hybridization.%5C+60%5C+individuals%5C+were%5C+collected%2C%5C+including%5C+putative%5C+parents%2C%5C+other%5C+4%5C+species%5C+of%5C+Ligularia%5C+nearby%2C%5C+putative%5C+hybrid%5C+A%5C+and%5C+B.%5C+They%5C+were%5C+all%5C+direct%5C+sequenced%5C+of%5C+four%5C+cpDNA%5C+fragments%2C%5C+and%5C+direct%5C+sequenced%5C+or%5C+cloning%5C+sequenced%5C+of%5C+nrDNA%5C+ITS4%5C-5.%5C+The%5C+results%5C+support%5C+that%5C+L.%5C+nelumbifolia%5C+and%5C+L.%5C+subspicata%5C+are%5C+parents%5C+of%5C+putative%5C+hybrid%5C+A%2C%5C+and%5C+the%5C+majority%5C+female%5C+parent%5C+is%5C+L.%5C+subspicata%2C%5C+L.%5C+vellerea%5C+may%5C+also%5C+be%5C+involved%5C+in%5C+the%5C+hybridization%5C+in%5C+some%5C+degree%5C%3B%5C+the%5C+nuclear%5C+sequences%5C+of%5C+putative%5C+hybrid%5C+B%5C+have%5C+no%5C+superposition%2C%5C+and%5C+its%5C+chloroplast%5C+DNA%5C+sequences%5C+are%5C+identical%5C+with%5C+L.%5C+nelumbifolia%2C%5C+so%5C+putative%5C+hybrid%5C+B%5C+could%5C+not%5C+be%5C+hybrid%5C%3B%5C+and%5C+there%5C+are%5C+backcross%5C+individuals%5C+exist%5C+among%5C+the%5C+putative%5C+parent%5C+L.%5C+subspicata.%5C+NewHybrids%5C+analysis%5C+of%5C+ISSR%5C+markers%5C+indicated%5C+that%2C%5C+the%5C+individuals%5C+of%5C+putative%5C+hybrid%5C+A%5C+are%5C+almost%5C+L.%5C+nelumbifolia%5C+and%5C+L.%5C+subspicata%5C+F1%5C+hybrid%5C+generation%5C+%5C%2810%5C%2F11%5C%29%2C%5C+only%5C+1%5C%2F11%5C+possibly%5C+backcross%5C+or%5C+other%5C+forms%5C%3B%5C+all%5C+individuals%5C+of%5C+hybrid%5C+B%5C+are%5C+L.%5C+nelumbifolia%5C%3B%5C+except%5C+one%5C+individual%5C+of%5C+L.%5C+subspicata%5C+as%5C+backcrossed%2C%5C+the%5C+other%5C+parent%5C+individuals%5C+are%5C+100%25%5C+reliable.%5C+This%5C+study%5C+focused%5C+on%5C+molecular%5C+evidence%2C%5C+complemented%5C+by%5C+ecological%2C%5C+reproductive%5C+and%5C+other%5C+characteristics%2C%5C+we%5C+demonstrated%5C+that%5C+the%5C+morphologically%5C+intermediate%5C+individuals%E2%80%99%5C+origin%2C%5C+and%5C+the%5C+probability%5C+of%5C+belonging%5C+to%5C+each%5C+parental%5C+or%5C+hybrid%5C+class.%5C+And%5C+concluded%5C+that%5C+L.%5C+nelumbifolia%5C+and%5C+L.%5C+subspicata%5C+are%5C+the%5C+parents%5C+of%5C+putative%5C+hybrid%5C+A%2C%5C+L.%5C+vellerea%5C+may%5C+also%5C+be%5C+involved%5C+in%5C+the%5C+hybridization%5C+in%5C+some%5C+degree%2C%5C+hybrids%5C+mainly%5C+are%5C+the%5C+first%5C+generation%2C%5C+a%5C+few%5C+individuals%5C+may%5C+be%5C+involved%5C+in%5C+backcross%2C%5C+and%5C+most%5C+probably%5C+backcross%5C+with%5C+L.%5C+subspicata%5C+according%5C+to%5C+the%5C+anthesis%2C%5C+while%5C+the%5C+assumption%5C+of%5C+hybrid%5C+B%5C+is%5C+not%5C+supported."},{"jsname":"In Chapter 1, we isolated a flavonoid prenyltransferase-like gene from traditional Chinese medicinal herb, Epimedium L. (berberidaceae). Epimedium species have a high content of the prenylated flavonol glycosides. Icariin and epimedin A, B and C are frequently used as marker compounds for the quality control of Epimedium. Here we speculate prenyl flavonoids biosynthesis pathway in Epimedium: The flavonoid prenyltransferase is responsible for the prenylation of flavonoids (naringenin 、kaempferol or apigenin) at the 8-position or 3'' or 5''-position. Leaves of Epimedium acuminatum Franch in the nursery were collected every month, and then detected the icariin content. The results show that leaves in March have the highest icariin content. Total RNA was extracted from leaves in March as template. A similarity-based cloning strategy yielded a flavonoid prenyltransferase-like gene, named EaPT1. In E. coli. expression system, pET32a(+) was chosen as the expression vector for use in Rosetta-gamiB(DE3)、RosettaTM 2（DE3）plysS、BL21(DE3)plysE、BL21(DE3)gold and BL21(DE3) cells. The full length ORF and truncated sequence were ligated with pET32a(+). We did not detect the target protein in SDS-PAGE. In Saccharomyces cerevisiae expression system, the full length ORF was ligated with pYES2. In this expression system, we still could not detect the protein in SDS-PAGE. LC/MS did not detect the activity of prenyltransferase, with naringenin as substrate. Chapter 2 describes functional expression and characterization of two copalyl pyrophosphate synthase gene from Isodon ericalyx (Dunn) Kudo, named IeCPS7 and IeCPS11. Their full length ORF and truncated sequence were ligated into pET32a(+). These vectors were used to transform E.coli BL21(DE)3. The truncated IeCPS7 sequence expressed a soluble His-tag recombinant protein, 104699.41D, pI5.87, 924aa. The recombinant protein was characterized for diterpene synthase activity by using geranylgeranyl diphosphate(GGPP) as substrates and subsequent GC/MS analysis of products. The purified recombinant IeCPS showed optimum activity at pH7.1. In addition, IeCPS showed maximum activity at 30℃. The enzymatic activity was increased by addition of MgCl2 to the reaction mixture. Unexpectedly, MnCl2 actually inhibited the enzyme activity. In addition,only insoluble recombinant proteins were expressed for IeCPS11 in BL21(DE)3, Rosetta-gamiB(DE3) and RosettaTM 2（DE3）plysS. The last part reviews the advances in molecular studies of aromatic prenyltransferase in plants and fungi, focusing on membrane-bound homogentisate prenyltransferses, flavonoid prenyltransferases as well as soluble indole prenyltransferases.","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&sort_by=2&isNonaffiliated=false&search_type=-1&query1=POLYMERASE-CHAIN-REACTION&order=desc&&fq=dc.project.title_filter%3AIn%5C+Chapter%5C+1%2C%5C+we%5C+isolated%5C+a%5C+flavonoid%5C+prenyltransferase%5C-like%5C+gene%5C+from%5C+traditional%5C+Chinese%5C+medicinal%5C+herb%2C%5C+Epimedium%5C+L.%5C+%5C%28berberidaceae%5C%29.%5C+Epimedium%5C+species%5C+have%5C+a%5C+high%5C+content%5C+of%5C+the%5C+prenylated%5C+flavonol%5C+glycosides.%5C+Icariin%5C+and%5C+epimedin%5C+A%2C%5C+B%5C+and%5C+C%5C+are%5C+frequently%5C+used%5C+as%5C+marker%5C+compounds%5C+for%5C+the%5C+quality%5C+control%5C+of%5C+Epimedium.%5C+Here%5C+we%5C+speculate%5C+prenyl%5C+flavonoids%5C+biosynthesis%5C+pathway%5C+in%5C+Epimedium%5C%3A%5C+The%5C+flavonoid%5C+prenyltransferase%5C+is%5C+responsible%5C+for%5C+the%5C+prenylation%5C+of%5C+flavonoids%5C+%5C%28naringenin%5C+%E3%80%81kaempferol%5C+or%5C+apigenin%5C%29%5C+at%5C+the%5C+8%5C-position%5C+or%5C+3%27%27%5C+or%5C+5%27%27%5C-position.%5C+Leaves%5C+of%5C+Epimedium%5C+acuminatum%5C+Franch%5C+in%5C+the%5C+nursery%5C+were%5C+collected%5C+every%5C+month%2C%5C+and%5C+then%5C+detected%5C+the%5C+icariin%5C+content.%5C+The%5C+results%5C+show%5C+that%5C+leaves%5C+in%5C+March%5C+have%5C+the%5C+highest%5C+icariin%5C+content.%5C+Total%5C+RNA%5C+was%5C+extracted%5C+from%5C+leaves%5C+in%5C+March%5C+as%5C+template.%5C+A%5C+similarity%5C-based%5C+cloning%5C+strategy%5C+yielded%5C+a%5C+flavonoid%5C+prenyltransferase%5C-like%5C+gene%2C%5C+named%5C+EaPT1.%5C+In%5C+E.%5C+coli.%5C+expression%5C+system%2C%5C+pET32a%5C%28%5C%2B%5C%29%5C+was%5C+chosen%5C+as%5C+the%5C+expression%5C+vector%5C+for%5C+use%5C+in%5C+Rosetta%5C-gamiB%5C%28DE3%5C%29%E3%80%81RosettaTM%5C+2%EF%BC%88DE3%EF%BC%89plysS%E3%80%81BL21%5C%28DE3%5C%29plysE%E3%80%81BL21%5C%28DE3%5C%29gold%5C+and%5C+BL21%5C%28DE3%5C%29%5C+cells.%5C+The%5C+full%5C+length%5C+ORF%5C+and%5C+truncated%5C+sequence%5C+were%5C+ligated%5C+with%5C+pET32a%5C%28%5C%2B%5C%29.%5C+We%5C+did%5C+not%5C+detect%5C+the%5C+target%5C+protein%5C+in%5C+SDS%5C-PAGE.%5C+In%5C+Saccharomyces%5C+cerevisiae%5C+expression%5C+system%2C%5C+the%5C+full%5C+length%5C+ORF%5C+was%5C+ligated%5C+with%5C+pYES2.%5C+In%5C+this%5C+expression%5C+system%2C%5C+we%5C+still%5C+could%5C+not%5C+detect%5C+the%5C+protein%5C+in%5C+SDS%5C-PAGE.%5C+LC%5C%2FMS%5C+did%5C+not%5C+detect%5C+the%5C+activity%5C+of%5C+prenyltransferase%2C%5C+with%5C+naringenin%5C+as%5C+substrate.%5C+Chapter%5C+2%5C+describes%5C+functional%5C+expression%5C+and%5C+characterization%5C+of%5C+two%5C+copalyl%5C+pyrophosphate%5C+synthase%5C+gene%5C+from%5C+Isodon%5C+ericalyx%5C+%5C%28Dunn%5C%29%5C+Kudo%2C%5C+named%5C+IeCPS7%5C+and%5C+IeCPS11.%5C+Their%5C+full%5C+length%5C+ORF%5C+and%5C+truncated%5C+sequence%5C+were%5C+ligated%5C+into%5C+pET32a%5C%28%5C%2B%5C%29.%5C+These%5C+vectors%5C+were%5C+used%5C+to%5C+transform%5C+E.coli%5C+BL21%5C%28DE%5C%293.%5C+The%5C+truncated%5C+IeCPS7%5C+sequence%5C+expressed%5C+a%5C+soluble%5C+His%5C-tag%5C+recombinant%5C+protein%2C%5C+104699.41D%2C%5C+pI5.87%2C%5C+924aa.%5C+The%5C+recombinant%5C+protein%5C+was%5C+characterized%5C+for%5C+diterpene%5C+synthase%5C+activity%5C+by%5C+using%5C+geranylgeranyl%5C+diphosphate%5C%28GGPP%5C%29%5C+as%5C+substrates%5C+and%5C+subsequent%5C+GC%5C%2FMS%5C+analysis%5C+of%5C+products.%5C+The%5C+purified%5C+recombinant%5C+IeCPS%5C+showed%5C+optimum%5C+activity%5C+at%5C+pH7.1.%5C+In%5C+addition%2C%5C+IeCPS%5C+showed%5C+maximum%5C+activity%5C+at%5C+30%E2%84%83.%5C+The%5C+enzymatic%5C+activity%5C+was%5C+increased%5C+by%5C+addition%5C+of%5C+MgCl2%5C+to%5C+the%5C+reaction%5C+mixture.%5C+Unexpectedly%2C%5C+MnCl2%5C+actually%5C+inhibited%5C+the%5C+enzyme%5C+activity.%5C+In%5C+addition%2Conly%5C+insoluble%5C+recombinant%5C+proteins%5C+were%5C+expressed%5C+for%5C+IeCPS11%5C+in%5C+BL21%5C%28DE%5C%293%2C%5C+Rosetta%5C-gamiB%5C%28DE3%5C%29%5C+and%5C+RosettaTM%5C+2%EF%BC%88DE3%EF%BC%89plysS.%5C+The%5C+last%5C+part%5C+reviews%5C+the%5C+advances%5C+in%5C+molecular%5C+studies%5C+of%5C+aromatic%5C+prenyltransferase%5C+in%5C+plants%5C+and%5C+fungi%2C%5C+focusing%5C+on%5C+membrane%5C-bound%5C+homogentisate%5C+prenyltransferses%2C%5C+flavonoid%5C+prenyltransferases%5C+as%5C+well%5C+as%5C+soluble%5C+indole%5C+prenyltransferases."},{"jsname":"International Science & Technology Innovation Cooperation program of China[2016YFE0126100]","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&sort_by=2&isNonaffiliated=false&search_type=-1&query1=POLYMERASE-CHAIN-REACTION&order=desc&&fq=dc.project.title_filter%3AInternational%5C+Science%5C+%5C%26%5C+Technology%5C+Innovation%5C+Cooperation%5C+program%5C+of%5C+China%5C%5B2016YFE0126100%5C%5D"},{"jsname":"Japan Society for the Promotion of Science[1264402271]","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&sort_by=2&isNonaffiliated=false&search_type=-1&query1=POLYMERASE-CHAIN-REACTION&order=desc&&fq=dc.project.title_filter%3AJapan%5C+Society%5C+for%5C+the%5C+Promotion%5C+of%5C+Science%5C%5B1264402271%5C%5D"},{"jsname":"K.C. Wong Magna Fund in Ningbo University","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&sort_by=2&isNonaffiliated=false&search_type=-1&query1=POLYMERASE-CHAIN-REACTION&order=desc&&fq=dc.project.title_filter%3AK.C.%5C+Wong%5C+Magna%5C+Fund%5C+in%5C+Ningbo%5C+University"},{"jsname":"Light of the West Talent Cultivation Program of Chinese Academy of Sciences[201684]","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&sort_by=2&isNonaffiliated=false&search_type=-1&query1=POLYMERASE-CHAIN-REACTION&order=desc&&fq=dc.project.title_filter%3ALight%5C+of%5C+the%5C+West%5C+Talent%5C+Cultivation%5C+Program%5C+of%5C+Chinese%5C+Academy%5C+of%5C+Sciences%5C%5B201684%5C%5D"},{"jsname":"lastIndexed","jscount":"2024-05-24"}],"资助项目","dc.project.title_filter")'>
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发表日期降序
题名升序
题名降序
提交时间升序
提交时间降序
Glabridin inhibits migration and invasion by transcriptional inhibition of matrix metalloproteinase-9 through modulation of NF-B and AP-1 activity in human liver cancer cells lines
期刊论文
出版物, 3111, 期号: 0, 页码: 1—28
作者:
Ming-Ju Hsieh
;
Chiao-Wen Lin
;
Shun-Fa Yang
;
Mu-Kuan Chen
;
Hui-Ling Chiou
Adobe PDF(1320Kb)
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浏览/下载：183/1
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提交时间：2017/07/24
Glabridin
Migration
Invasion
Matrix Metalloproteinase-9
Hepatoma
Boletes clarified
期刊论文
出版物, 3111, 期号: 0, 页码: 1-38
作者:
David Arora
;
Jonathan L. Frank
Adobe PDF(1003Kb)
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浏览/下载：247/1
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提交时间：2017/07/24
Appendiculati
Boletaceae
Butter Boletes
Butyriboletus
Molecular phylogenetics
New Genus
New Species
Taxonomy
Olmesartan prevents cardiac rupture in mice with myocardial infarction by modulating growth differentiation factor 15 and P53
期刊论文
出版物, 3111, 期号: 0, 页码: 1-37
作者:
Baihe Chen
;
Di Lu
;
Yujuan Fu
;
Jingwen Zhang
;
Xiaobo Huang
;
Shiping Cao
;
Dingli Xu
;
Jianping Bin
;
Masafumi Kitakaze
;
Qiaobing Huang
;
Yulin Liao
Adobe PDF(1769Kb)
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浏览/下载：286/1
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提交时间：2017/07/24
Cardiac Rupture
Olmesartan
P53
Gdf-15
Aldosterone
Myocardial Infarction
Resveratrol Decrease Fructose-Induced Oxidative Stress Mediated by NADPH Oxidase via an AMPK-Dependent Mechanism1
期刊论文
出版物, 3111, 期号: 0, 页码: 1-44
作者:
Pei-Wen Cheng, PhD
;
Wen-Yu Ho, MD, PhD
;
Yu-Ting Su, MS
;
Pei-Jung Lu,PhD
;
Bo-Zone Chen MS
;
Wen-Han Cheng, MS
;
Wen-Hsien Lu, MD, MS
;
Gwo-Ching Sun, MD
;
Tung-Chen Yeh, MD
;
Michael Hsiao DVM, PhD
;
Ching-Jiunn Tseng, MD, PhD
Adobe PDF(1555Kb)
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浏览/下载：131/1
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提交时间：2017/07/24
Ampk
Hypertension
Nucleus Tractus Solitarii
Nitrogen Oxides
Resveratrol
A Multiplex PCR System of Novel Microsatellite Loci for Population Genetic Application in Walnuts
期刊论文
PLANTS-BASEL, 2023, 卷号: 12, 期号: 24, 页码: 4101
作者:
Xiahou,Zuo-Ying
;
Wambulwa,Moses C.
;
Xu,Zu-Chang
;
Ye,Lin-Jiang
;
Fan,Peng-Zhen
;
Magige,Ephie A.
;
Luo,Ya-Huang
;
Liu,Jie
Adobe PDF(2472Kb)
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浏览/下载：4/1
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提交时间：2024/05/09
genetic diversity
Juglans
microsatellite marker
multiplex PCR
transferability
walnut
CROSS-SPECIES AMPLIFICATION
SSR-MARKERS
JUGLANS-MANDSHURICA
COMPUTER-PROGRAM
DIVERSITY
SOFTWARE
SUCCESS
ASSAY
Anti-Inflammatory Effects of Camellia fascicularis Polyphenols via Attenuation of NF-kappa B and MAPK Pathways in LPS-Induced THP-1 Macrophages
期刊论文
JOURNAL OF INFLAMMATION RESEARCH, 2022, 卷号: 15, 页码: 851-864
作者:
Gao, Miaozi
;
Peng, Xiaowei
;
Tang, Junrong
;
Deng, Jia
;
Wang, Fang
;
Zhang, Yingjun
;
Zhao, Ping
;
Kan, Huan
;
Liu, Yun
Adobe PDF(9145Kb)
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浏览/下载：23/7
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提交时间：2024/04/30
Camellia fascicularis
polyphenols
anti-inflammatory activity
NF-kappa B
MAPK
LIPOPOLYSACCHARIDE-INDUCED INFLAMMATION
OXIDATIVE STRESS
CELLS
SUPPRESSION
ANTIOXIDANT
ACTIVATION
NRF2/HO-1
RESPONSES
PROTEIN
CANCER
Selection and Validation of Reference Genes for Quantitative Real-Time PCR Analysis of Development and Tissue-Dependent Flower Color Formation in Cymbidium lowianum
期刊论文
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2022, 卷号: 23, 期号: 2, 页码: 738
作者:
Dong, Xiu-Mei
;
Zhang, Wei
;
Zhang, Shi-Bao
Adobe PDF(2940Kb)
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浏览/下载：16/6
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提交时间：2024/04/30
Cymbidium lowianum
flower organ development
reference gene selection and validation
qRT-PCR
transcriptome data
geNorm and Normfinder software
POLYMERASE-CHAIN-REACTION
EXPRESSION
NORMALIZATION
QUANTIFICATION
Selection of appropriate reference genes in different tissues of Vaccinium dunalianum Wight by quantitative real-time PCR for gene expression studies
期刊论文
CANADIAN JOURNAL OF PLANT SCIENCE, 2022, 卷号: 102, 期号: 1, 页码: 207-215
作者:
Shao, Yalin
;
Chen, Aiyi
;
Chang, Wei
;
Li, Guoze
;
Yu, Jinde
;
Zhao, Ping
;
Ding, Yong
Adobe PDF(2111Kb)
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浏览/下载：4/2
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提交时间：2024/04/30
EIF-4A-2
PP2A-2
qRT-PCR
reference genes
Vaccinium dunalianum Wight
CANDIDATE REFERENCE GENES
CHLOROGENIC ACID
IDENTIFICATION
ARBUTIN
NORMALIZATION
QPCR
金钗石斛copacamphane型倍半萜合成酶的探究与应用
学位论文
, 2021
作者:
刘立
Adobe PDF(6133Kb)
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浏览/下载：9/0
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提交时间：2024/03/20
Morphological and Phylogenetic Appraisal of Novel and Extant Taxa of Stictidaceae from Northern Thailand
期刊论文
JOURNAL OF FUNGI, 2021, 卷号: 7, 期号: 10, 页码: 880
作者:
Wei,De-Ping
;
Wanasinghe,Dhanushka N.
;
Gentekaki,Eleni
;
Thiyagaraja,Vinodhini
;
Lumyong,Saisamorn
;
Hyde,Kevin D.
Adobe PDF(3951Kb)
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浏览/下载：87/30
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提交时间：2022/04/02
lichenization
new species
non-lichenized fungi
Ostropales
phylogeny
taxonomy
LICHENICOLOUS FUNGI
ASCOMYCOTA
OSTROPALES
STICTIS
PLACEMENT
KEY
ODONTOTREMATACEAE
CLASSIFICATION
ABSCONDITELLA
COMBINATIONS
