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Anti-Inflammatory Effects of Camellia fascicularis Polyphenols via Attenuation of NF-kappa B and MAPK Pathways in LPS-Induced THP-1 Macrophages
Gao, Miaozi; Peng, Xiaowei; Tang, Junrong; Deng, Jia; Wang, Fang; Zhang, Yingjun; Zhao, Ping; Kan, Huan; Liu, Yun
2022
Source PublicationJOURNAL OF INFLAMMATION RESEARCH
Volume15Pages:851-864
AbstractPurpose: Plant polyphenols possess beneficial functions against various diseases. This study aimed to identify phenolic ingredients in Camellia fascicularis (C. fascicularis) and investigate its possible underlying anti-inflammatory mechanism in lipopolysaccharide (LPS)-induced human monocytes (THP-1) macrophages. Methods: C. fascicularis polyphenols (CFP) were characterized by ultra-performance liquid chromatography (UPLC) combined with quadrupole-time-of-flight mass/mass spectrometry (Q-TOF-MS/MS). The THP-1 cells were differentiated into macrophages under the stimulation of phorbol 12-myristate 13-acetate (PMA) and then treated with LPS to build a cellular inflammation model. The cell viability was detected by CCK-8 assay. The levels of reactive oxygen species (ROS) were assessed by flow cytometry. The secretion and expression of inflammatory cytokines were tested by enzyme-linked immunosorbent assay (ELISA) and real-time polymerase chain reaction (RT-PCR). In addition, the nuclear factor-kappa B (NF-kappa B) and mitogen-activated protein kinase (MAPK) signaling pathways were analyzed by Western blotting. Results: Twelve phenolic constituents including (-)-epicatechin, casuariin, agastachoside, etc. in CFP were identified. The CCK-8 assay showed that CFP exhibited no significant cytotoxicity between 100 and 300 mu g/mL. After treated with CFP, the release of ROS was significantly suppressed. CFP inhibited inflammation in macrophages by attenuating the polarization of LPS-induced THP-1 macrophages, down-regulating the expression of the pro-inflammatory cytokines IL-6, IL-1 beta and TNF-alpha, and up-regulating the expression of the anti-inflammatory cytokine IL-10. Western blotting experiments manifested that CFP could markedly inhibit the phosphorylation of p65, ERK and JNK, thereby suppressing the activation of NF-kappa B and MAPK signaling pathways. Conclusion: These findings indicated that CFP exerted anti-inflammatory activity by inhibiting the activation NF-kappa B and MAPK pathways which may induce the secretion of pro-inflammatory cytokines. This study offers a reference for C. fascicularis as the source of developing natural, safe anti-inflammatory agents in the future.
KeywordCamellia fascicularis polyphenols anti-inflammatory activity NF-kappa B MAPK LIPOPOLYSACCHARIDE-INDUCED INFLAMMATION OXIDATIVE STRESS CELLS SUPPRESSION ANTIOXIDANT ACTIVATION NRF2/HO-1 RESPONSES PROTEIN CANCER
DOI10.2147/JIR.S349981
Indexed BySCI
WOS IDWOS:000754259600002
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Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/74701
Collection中国科学院昆明植物研究所
Recommended Citation
GB/T 7714
Gao, Miaozi,Peng, Xiaowei,Tang, Junrong,et al. Anti-Inflammatory Effects of Camellia fascicularis Polyphenols via Attenuation of NF-kappa B and MAPK Pathways in LPS-Induced THP-1 Macrophages[J]. JOURNAL OF INFLAMMATION RESEARCH,2022,15:851-864.
APA Gao, Miaozi.,Peng, Xiaowei.,Tang, Junrong.,Deng, Jia.,Wang, Fang.,...&Liu, Yun.(2022).Anti-Inflammatory Effects of Camellia fascicularis Polyphenols via Attenuation of NF-kappa B and MAPK Pathways in LPS-Induced THP-1 Macrophages.JOURNAL OF INFLAMMATION RESEARCH,15,851-864.
MLA Gao, Miaozi,et al."Anti-Inflammatory Effects of Camellia fascicularis Polyphenols via Attenuation of NF-kappa B and MAPK Pathways in LPS-Induced THP-1 Macrophages".JOURNAL OF INFLAMMATION RESEARCH 15(2022):851-864.
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