Selection of appropriate reference genes in different tissues of Vaccinium dunalianum Wight by quantitative real-time PCR for gene expression studies

doi:10.1139/cjps-2020-0302

KIB OpenIR

	Selection of appropriate reference genes in different tissues of Vaccinium dunalianum Wight by quantitative real-time PCR for gene expression studies
	Shao, Yalin; Chen, Aiyi; Chang, Wei; Li, Guoze; Yu, Jinde; Zhao, Ping; Ding, Yong
	2022
发表期刊	CANADIAN JOURNAL OF PLANT SCIENCE
卷号	102 期号:1 页码:207-215
摘要	Quantitative real-time polymerase chain reaction (PCR) is a powerful tool for investigating relevant changes in gene expression. At present, there are no reports on reference genes for Vaccinium dunalianum Wight (V. dunalianum Wight). Here, the expression profiles of 15 frequently used reference genes were used to screen for appropriate reference genes in different tissues of V. dunalianum Wight collected in Yunnan and Wuding, China. The cycle threshold (Ct) values of genes in different samples were compared and analyzed. Furthermore, BestKeeper, NormFinder, and geNorm software were used to evaluate the stability of each candidate reference gene. Analysis using geNorm showed that the expression levels of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and protein phosphatase 2A-2 (PP2A-2) were the most stable. In turn, the expression level of eukaryotic initiation factor-2 (EIF-4A-2) was the most stable as per analysis by NormFinder, while Bestkeeper revealed that actin-related protein-1 (ARP-1) was the most stably expressed. Comprehensive analysis and validation experiments showed that EIF-4A-2 and PP2A-2 were the most suitable reference genes for V. dunalianum Wight.
关键词	EIF-4A-2 PP2A-2 qRT-PCR reference genes Vaccinium dunalianum Wight CANDIDATE REFERENCE GENES CHLOROGENIC ACID IDENTIFICATION ARBUTIN NORMALIZATION QPCR
DOI	10.1139/cjps-2020-0302
收录类别	SCI
WOS记录号	WOS:000754692500018
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/74730
专题	中国科学院昆明植物研究所
推荐引用方式 GB/T 7714	Shao, Yalin,Chen, Aiyi,Chang, Wei,et al. Selection of appropriate reference genes in different tissues of Vaccinium dunalianum Wight by quantitative real-time PCR for gene expression studies[J]. CANADIAN JOURNAL OF PLANT SCIENCE,2022,102(1):207-215.
APA	Shao, Yalin.,Chen, Aiyi.,Chang, Wei.,Li, Guoze.,Yu, Jinde.,...&Ding, Yong.(2022).Selection of appropriate reference genes in different tissues of Vaccinium dunalianum Wight by quantitative real-time PCR for gene expression studies.CANADIAN JOURNAL OF PLANT SCIENCE,102(1),207-215.
MLA	Shao, Yalin,et al."Selection of appropriate reference genes in different tissues of Vaccinium dunalianum Wight by quantitative real-time PCR for gene expression studies".CANADIAN JOURNAL OF PLANT SCIENCE 102.1(2022):207-215.

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