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中国科学院昆明植物研究所知识管理系统
Knowledge Management System of Kunming Institute of Botany,CAS
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付艳辉 [1]
王豪 [1]
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何俊 [1]
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GST, P < 0.05) were exhibited by this species. The SAMOVA revealed seven diverging groups of related chlorotypes, six of them had distinct nonoverlapping geographical ranges: one in the northeast comprising 10 populations, a second with a southeast distribution comprising 22 populations, and the remaning four groups comprising 15 populations located in the west part of the species’ range along different river valleys. The genetic clustering of populations into three regions was also supported by analysis of molecular variance, which showed that most genetic variation (82.43%) was found among these three regions. Two clusters were distinguished by both phylogenetic analysis and genealogical analysis of chlorotypes, one consisting of chlorotypes from the western region and the second consisting of those from the eastern region. Significant genetic differences between the two regions might be attributed to vicariance and restricted gene flow, and this vicariance could be explained by the physical environmental heterogeneity on each side of the Tanaka-Kaiyong Line. Following the uplift of the Tibetan Plateau, the reorganization of the major river drainages was primarily caused by river separation and capture events. These historical events could change the distribution of S. davidii from fragmented to continuous (Upper/Lower Jinshajiang and Yalongjiang/Daduhe), and from continuous to fragmented (Nujiang and Jinshajiang/Honghe). However, spatial and temporal patterns of phylogeographic divergence are strongly associated with historical disjunction rather than modern drainage connections. Moreover, the following north-south split in the eastern region and effective isolation with their genetic diversity were essentially modelled by genetic drift. The higher chlorotype richness and genetic divergence for populations in western region compared with other two regions suggests that there were multipe refugia or in situ survival of S. davidii in the Himalayan-Hengduan Mountain region. Fixation of chlorotypes in the northeastern region and near fixation in the southeastern region suggest a recent colonization of these areas. We further found that this species underwent past range expansion around 37-303 thousand years ago (kya). The southeastern populations likely experienced a demographic expansion via unidirectional gene flow along rivers, while northeastern populations underwent a more northward expansion, both from initial populations (s) (21, 22, 23) preserved on eastern refugia (Jinshajiang). This process might have been accompanied with a series of founder effects or bottlenecks making populations genetically impoverished. 3. Phylogeographic analysisbased on nuclear sequence，We sequenced the nuclear (ncpGS) region in all populations sampled, recovering 23 nuclear haplotypes. Compared to cpDNA, both NST (0.470) and GST (0.338) were relatively lower, but NST was also significantly larger than GST. 37.10% of the total variation was distributed among regions which was much lower than that shown by chlorotypes. Thus, more extensive distribution of nuclear haplotypes was exhibited across the geographical range instead of the strong population subdivision observed in chlorotypes. Similarly to the chloroplast data, we found that genetic differentiation of nDNA was positively correlated with the geographical distance, but the increase in the geographical distance between populations did not increase the genetic differentiation of nDNA as rapidly as that of cpDNA. These contrasting levels between the chloroplast and nuclear genomes of S. davidii are likely due to limited gene flow of cpDNA by seeds vs. the extensive gene flow of nDNA by wind-mediated pollen in the population history. We also determined from nuclear markers that haplotype diversity was reduced in the southeastern and northeastern regions due to the loss of rare haplotypes in western region. This reduction of gene diversity is also a signature of founder events or recent bottleneck during post-glacial colonization. However, nuclear diversity within populations remains high. This provides evidence that regionally pollen flow might be sufficiently high to blur the genetic identity of founder populations over a reasonably large spatial scale.3. Relationships among three varieties，The phylogenetic analysis identified two phylogroups of chlorotypes, corresponding to S. davidii var. davidii and var. chuansinesis. The former was distinguished by the abscence of predonminant nuclear haplotype H1 of the latter. The monophyletic group of chlorotypes in var. davidii and var. liangshanesis showed their relatively close relationship. And their genetic divergence from the third variety appears to be relative to their slight morphological difference in leaf size and the divergent environmental niche spaces they occupy. Thus, the observed differences in morphological characters between var. chuansinesis and other two varieties can be explained by the seed dispersal limitation illustrated above (as inferred by geographical separation) and by environmental heterogeneity (as inferred by precipitation or elevation) or by a combination of both. After all, the geological changes, drainage reorganization, and floristic differences following the Himalayan uplift have been suggested to affect the genetic structure of S. davidii. These results provide new insights into the phylogeographic pattern of plants in China. In addition, the unique population genetic structure found in S. davidii has provided important insights into the evolutionary history of this species. The genetic profile uncovered in this study is also critical for its conservation management. Our study has uncovered the existence of at least two ‘evolutionary significant units’ independent units within S. davidii, corresponding to var. davidii from eastern region and var. chuansinensis from western region. The conservation efforts should first focus on most western populations and on the southeastern ones exhibiting high levels of genetic diversity, while the genetically homogeneous northeastern populations located in the degraded Loess Plateau should require much greater conservation 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专题：昆明植物所硕博研究生毕业学位论文
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蕊木和奶子藤中生物碱类化学成分及其生物活性评价
学位论文
: 中国科学院大学, 2022
作者:
陈谌
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提交时间：2024/05/14
夹竹桃科
Apocynaceae
蕊木
Kopsia arborea
奶子藤
Bousigonia mekongensis
单萜吲哚生物碱
Monoterterpene indole alkaloids
生物活性
Biological activity
三尖杉属植物生物碱成分研究和活性酯碱资源评价
学位论文
: 中国科学院大学, 2022
作者:
余光兴
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提交时间：2024/05/14
三尖杉属,种子,三尖杉生物碱,分离鉴定,酯碱资源评价
Cephalotaxus,Seeds,Cephalotaxine alkaloids,Isolation and identification,Ester alkaloids resources evaluation
泽泻醇Alisols A/B的结构修饰与石松生物碱Huperserratines A/B的全合成研究
学位论文
: 中国科学院大学, 2022
作者:
邓振涛
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提交时间：2024/05/14
泽泻醇 B，NAFLD，石松生物碱，Huperserratine A，全合成
Alisol B, NAFLD, Lycopodium alkaloids, Huperserratine A, Total synthesis
青风藤、北豆根和防己的生物碱成分及生物活性
学位论文
: 中国科学院大学, 2022
作者:
毕冉
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提交时间：2024/05/14
防己科，青风藤，北豆根，防己，生物碱，生物活性，青藤碱
Menispermaceae, Sinomenii Caulis, Menispermi Rhizoma, Stephaniae Tetrandrae Radix, Alkaloids, Bioactivities, Sinomenine
山地香茶菜的化学成分及其生物活性研究
学位论文
: 中国科学院大学, 2022
作者:
邱彩玲
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山地香茶菜，二萜，细胞毒，促进溶酶体功能
Isodon oresbius, Diterpenoids, Cytotoxicity, Promotion of lysosomal function
茵陈蒿和牛尾蒿的抗肝纤维化与抗肝癌活性成分研究
学位论文
: 中国科学院大学, 2022
作者:
高振
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提交时间：2024/05/14
茵陈蒿，牛尾蒿，茵陈香豆酸，倍半萜二聚体，倍半萜，抗肝纤维化活性，抗肝癌活性，细胞周期，凋亡
Artemisia capillaris, Artemisia dubia, Coumaric acids, Sesquiterpenoids, Sesquiterpenoid dimers, Antihepatic fibrosis effects, Antihepatoma activity, Cell cycle, Apoptosis
海菜花的化学成分及其生物活性研究
学位论文
: 中国科学院大学, 2022
作者:
刘洪星
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提交时间：2024/05/14
海菜花，Ottelione，二芳基庚烷，糖尿病，抗肿瘤
Ottelia acuminata, Ottelione, Diarylheptanoids, DM, Antitumor
西印度醋栗果实的化学成分研究
学位论文
: 中国科学院大学, 2022
作者:
胥佳
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提交时间：2024/05/14
大戟科，西印度醋栗，化学成分，生物活性，营养分析
Euphorbiaceae, Phyllanthus acidus (L.) Skeels, Chemical constituents, Biological activity, Nutritional analysis
松叶蕨和翠云草化学成分研究
学位论文
: 中国科学院大学, 2022
作者:
周志萍
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提交时间：2024/05/14
松叶蕨
Psilotum nudum (L.) Beauv
翠云草
Selaginella uncinata (Desv.) Spring
化学成分
Chemical compositions
细胞毒活性
Cytotoxic activity
NO生成抑制活性
NO inhibitory activity
玛咖活性成分及其不同加工方式下化学成分变化的研究
学位论文
: 中国科学院大学, 2022
作者:
张然然
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浏览/下载：9/0
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提交时间：2024/05/14
十字花科，玛咖，硫代乙内酰脲，生物活性， UPLC-MS/MS-MRM，成分分析
Cruciferous, Lepidium meyenii, Thiohydantoin, Bioactivity, UPLCMS/MS-MRM, Analysis of chemical constituents
