Nematicidal Activity of 20-Deoxyingenol-3-angelate from Euphorbia peplus Latex Through Protein Kinase C Isotype TPA-1

doi:10.1021/acs.jafc.3c07861

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	Nematicidal Activity of 20-Deoxyingenol-3-angelate from Euphorbia peplus Latex Through Protein Kinase C Isotype TPA-1
	Mu, Rong-Fang; Hua, Juan; Li, Dan; Luo, Shi-Hong; Chen, Yue-Gui; Jing, Shu-Xi; Liu, Yan; Li, Sheng-Hong
	2024
发表期刊	JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
ISSN	0021-8561
卷号	72 期号:17 页码:9746-9754
摘要	The latex of Euphorbia peplus and its major component 20-deoxyingenol-3-angelate (DI3A) displayed significant nematicidal activity against Caenorhabditis elegans and Panagrellus redivivus. DI3A treatment inhibited the growth and development of nematodes and caused significantly negative effects on locomotion behavior, reproduction, and accumulation of reactive oxygen species. Transcriptome analysis indicated that differential expression genes in DI3A-treated C. elegans were mainly associated with the metabolism, growth, and development process, which were further confirmed by RT-qPCR experiments. The expression level of TPA-1 gene encoding a protein kinase C isotype was obviously upregulated by DI3A treatment, and knockdown of TPA-1 by RNAi technology in the nematode could relieve the growth-inhibitory effect of DI3A. Metabolic analysis indicated that DI3A was hardly metabolized by C. elegans, but a glycosylated indole derivative was specifically accumulated likely due to the activation of detoxification. Overall, our findings suggested that DI3A from E. peplus latex exerted a potent nematicidal effect through the gene TPA-1, which provides a potential target for the control of nematodes and also suggests the potential application value of E. peplus latex and DI3A as botanical nematicides.
DOI	10.1021/acs.jafc.3c07861
WOS记录号	WOS:001201280100001
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/76296
专题	中国科学院昆明植物研究所
推荐引用方式 GB/T 7714	Mu, Rong-Fang,Hua, Juan,Li, Dan,et al. Nematicidal Activity of 20-Deoxyingenol-3-angelate from Euphorbia peplus Latex Through Protein Kinase C Isotype TPA-1[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2024,72(17):9746-9754.
APA	Mu, Rong-Fang.,Hua, Juan.,Li, Dan.,Luo, Shi-Hong.,Chen, Yue-Gui.,...&Li, Sheng-Hong.(2024).Nematicidal Activity of 20-Deoxyingenol-3-angelate from Euphorbia peplus Latex Through Protein Kinase C Isotype TPA-1.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,72(17),9746-9754.
MLA	Mu, Rong-Fang,et al."Nematicidal Activity of 20-Deoxyingenol-3-angelate from Euphorbia peplus Latex Through Protein Kinase C Isotype TPA-1".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 72.17(2024):9746-9754.

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