Testing and using complete plastomes for authentication of medicinal Polygonatum species (Asparagaceae)

doi:10.1016/j.indcrop.2023.116557

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	Testing and using complete plastomes for authentication of medicinal Polygonatum species (Asparagaceae)
	Wang,Shuying; Zhou,Nian; Shi,Naixing; Zhang,Guangfei; Liu,Haiyang; Guo,Xiaorong; Ji,Yunheng
	2023
发表期刊	INDUSTRIAL CROPS AND PRODUCTS
ISSN	1872-633X
卷号	197 页码:116557
摘要	Polygonati Rhizoma, a traditional Chinese medicine important to the pharmaceutical and food industries, is frequently adulterated with morphologically similar congeneric species, posing quality and effectiveness issues for the products. Accordingly, the accurate and sensitive authentication of medicinal Polygonatum species is vital to the standardization of Polygonati Rhizoma products. In view of the difficulties in morphological analysis and the use of standard DNA barcodes for authentication of medicinal Polygonatum species, an alternative authen-tication method is needed. Based on inter-and intraspecies sampling, phylogenetic and genetic distance analyses were performed to evaluate the discriminative power of complete plastomes for the identification of Polygonatum species. The results indicate that the genetic criteria of complete plastomes are in good correspondence with species boundaries and therefore can be used for unambiguous identification of medicinal Polygonatum species and morphologically similar congeners, which verifies the theoretical prediction that the application of genome skimming to generate complete plastomes as molecular tags can serve as an effective and accurate method to detect whether Polygonati Rhizoma is adulterated with congeneric species. Additionally, in this study, a taxo-nomically well-curated and high-resolution reference plastome superbarcode library for Polygonatum was developed for the first time, providing a valuable genetic resource for the application of genome skimming to monitor and control the production and trade of Polygonati Rhizoma to guarantee the effectiveness of down-stream products. The findings also provide new insights to clarify the taxonomic ambiguities of the generic circumscription of Polygonatum and Heteropolygonatum, as well as the species delimitation of Polygonatum cyrtonema, Polygonatum nodosum, and Polygonatum punctatum, which will contribute to proper exploitation and conservation of Polygonatum species.
关键词	Adulterant Genome skimming Plant barcoding 2 0 Polygonati Rhizoma Species identification DNA BARCODE IQ-TREE GENOMES REINSTATEMENT
学科领域	Agriculture
DOI	10.1016/j.indcrop.2023.116557
收录类别	SCI
WOS记录号	WOS:000955743400001
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/75569
专题	中国科学院昆明植物研究所
推荐引用方式 GB/T 7714	Wang,Shuying,Zhou,Nian,Shi,Naixing,et al. Testing and using complete plastomes for authentication of medicinal Polygonatum species (Asparagaceae)[J]. INDUSTRIAL CROPS AND PRODUCTS,2023,197:116557.
APA	Wang,Shuying.,Zhou,Nian.,Shi,Naixing.,Zhang,Guangfei.,Liu,Haiyang.,...&Ji,Yunheng.(2023).Testing and using complete plastomes for authentication of medicinal Polygonatum species (Asparagaceae).INDUSTRIAL CROPS AND PRODUCTS,197,116557.
MLA	Wang,Shuying,et al."Testing and using complete plastomes for authentication of medicinal Polygonatum species (Asparagaceae)".INDUSTRIAL CROPS AND PRODUCTS 197(2023):116557.

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