Discovery of ACE Inhibitory Peptides Derived from Green Coffee Using In Silico and In Vitro Methods

doi:10.3390/foods12183480

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	Discovery of ACE Inhibitory Peptides Derived from Green Coffee Using In Silico and In Vitro Methods
	Dai,Haopeng; He,Min; Hu,Guilin; Li,Zhongrong; Al-Romaima,Abdulbaset; Wu,Zhouwei; Liu,Xiaocui; Qiu,Minghua
	2023
发表期刊	FOODS
ISSN	2304-8158
卷号	12 期号:18 页码:3480
摘要	Inhibition of angiotensin-I converting enzyme (ACE) is an important means of treating hypertension since it plays an important regulatory function in the renin-angiotensin system. The aim of this study was to investigate the ACE inhibitory effect of bioactive peptides from green coffee beans using in silico and in vitro methods. Alcalase and thermolysin were employed to hydrolyze protein extract from coffee beans. Bioactive peptides were identified by LC-MS/MS analysis coupled with database searching. The potential bioactivities of peptides were predicted by in silico screening, among which five novel peptides may have ACE inhibitory activity. In vitro assay was carried out to determine the ACE inhibitory degree. Two peptides (IIPNEVY, ITPPVMLPP) were obtained with IC50 values of 57.54 and 40.37 & mu;M, respectively. Furthermore, it was found that two inhibitors bound to the receptor protein on similar sites near the S1 active pocket of ACE to form stable enzyme-peptide complexes through molecular docking, and the Lineweaver-Burk plot showed that IIPNEVY was a noncompetitive inhibitor, and ITPPVMLPP was suggested to be a mixed-type inhibitor. Our study demonstrated that two peptides isolated from coffee have potential applications as antihypertensive agents.
关键词	coffee protein peptide ACE inhibitor LC-MS/MS molecular docking ANGIOTENSIN-CONVERTING ENZYME IDENTIFICATION
DOI	10.3390/foods12183480
WOS记录号	WOS:001073981100001
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/75009
专题	中国科学院昆明植物研究所
推荐引用方式 GB/T 7714	Dai,Haopeng,He,Min,Hu,Guilin,et al. Discovery of ACE Inhibitory Peptides Derived from Green Coffee Using In Silico and In Vitro Methods[J]. FOODS,2023,12(18):3480.
APA	Dai,Haopeng.,He,Min.,Hu,Guilin.,Li,Zhongrong.,Al-Romaima,Abdulbaset.,...&Qiu,Minghua.(2023).Discovery of ACE Inhibitory Peptides Derived from Green Coffee Using In Silico and In Vitro Methods.FOODS,12(18),3480.
MLA	Dai,Haopeng,et al."Discovery of ACE Inhibitory Peptides Derived from Green Coffee Using In Silico and In Vitro Methods".FOODS 12.18(2023):3480.

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