Polyamine metabolism links gut microbiota and testicular dysfunction

doi:10.1186/s40168-021-01157-z

KIB OpenIR

	Polyamine metabolism links gut microbiota and testicular dysfunction
	Zhao,Qi; Huang,Jian-Feng ; Cheng,Yan ; Dai,Man-Yun ; Zhu,Wei-Feng ; Yang,Xiu-Wei ; Gonzalez,Frank J.; Li,Fei
	2021
发表期刊	MICROBIOME
ISSN	2049-2618
卷号	9 期号:1 页码:224
摘要	Background: Male fertility impaired by exogenous toxins is a serious worldwide issue threatening the health of the new-born and causing infertility. However, the metabolic connection between toxic exposures and testicular dysfunction remains unclear.Results: In the present study, the metabolic disorder of testicular dysfunction was investigated using triptolide-induced testicular injury in mice. We found that triptolide induced spermine deficiency resulting from disruption of polyamine biosynthesis and uptake in testis, and perturbation of the gut microbiota. Supplementation with exogenous spermine reversed triptolide-induced testicular dysfunction through increasing the expression of genes related to early and late spermatogenic events, as well as increasing the reduced number of offspring. Loss of gut microbiota by antibiotic treatment resulted in depletion of spermine levels in the intestine and potentiation of testicular injury. Testicular dysfunction in triptolide-treated mice was reversed by gut microbial transplantation from untreated mice and supplementation with polyamine-producing Parabacteroides distasonis. The protective effect of spermine during testicular injury was largely dependent on upregulation of heat shock protein 70s (HSP70s) both in vivo and in vitro.Conclusions: The present study linked alterations in the gut microbiota to testicular dysfunction through disruption of polyamine metabolism. The diversity and dynamics of the gut microbiota may be considered as a therapeutic option to prevent male infertility.
关键词	Testicular dysfunction Gut microbiota Polyamine metabolism Metabolomics SMALL-MOLECULE INHIBITOR ORNITHINE-DECARBOXYLASE TRIPTOLIDE SPERMINE ACTIVATION DISRUPTION MECHANISMS EXPRESSION APOPTOSIS TRANSPORT
DOI	10.1186/s40168-021-01157-z
WOS记录号	WOS:000716997100002
引用统计	被引频次：36[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/73530
专题	中国科学院昆明植物研究所
作者单位	1.[Zhao, Qi 2.Cheng, Yan 3.Dai, Man-Yun 4.Sichuan Univ, West China Hosp, Frontiers Sci Ctr Dis Related Mol Network, State Key Lab Biotherapy, Chengdu 610041, Peoples R China 5.Huang, Jian-Feng 6.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China 7.Shanwei Inst Food & Drug Control, Shanwei 516622, Guangdong, Peoples R China 8.Jiangxi Univ Tradit Chinese Med, Academician Workstn, Nanchang 330004, Jiangxi, Peoples R China 9.Peking Univ, Hlth Sci Ctr, Sch Pharmaceut Sci, Beijing 100191, Peoples R China 10.[Gonzalez, Frank J.] NCI, Lab Metab, Ctr Canc Res, NIH, Bethesda, MD 20892 USA
推荐引用方式 GB/T 7714	Zhao,Qi,Huang,Jian-Feng,Cheng,Yan,et al. Polyamine metabolism links gut microbiota and testicular dysfunction[J]. MICROBIOME,2021,9(1):224.
APA	Zhao,Qi.,Huang,Jian-Feng.,Cheng,Yan.,Dai,Man-Yun.,Zhu,Wei-Feng.,...&Li,Fei.(2021).Polyamine metabolism links gut microbiota and testicular dysfunction.MICROBIOME,9(1),224.
MLA	Zhao,Qi,et al."Polyamine metabolism links gut microbiota and testicular dysfunction".MICROBIOME 9.1(2021):224.