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Characterization of defensive cadinenes and a novel sesquiterpene synthase responsible for their biosynthesis from the invasive Eupatorium adenophorum
Liu,Yan; Luo,Shi-Hong; Hua,Juan; Li,De-Sen; Ling,Yi; Luo,Qian; Li,Sheng-Hong
2021
Source PublicationNEW PHYTOLOGIST
ISSN0028-646X
Volume229Issue:3Pages:1740-1754
AbstractEupatorium adenophorumis a malignant invasive plant possessing extraordinary defense potency, but its chemical weaponry and formation mechanism have not yet been extensively investigated.We identified six cadinene sesquiterpenes, including two volatiles (amorpha-4,7(11)-diene and (-)-amorph-4-en-7-ol) and four nonvolatiles (9-oxo-10,11-dehydroageraphorone, muurol-4-en-3,8-dione, 9-oxo-ageraphorone and 9 beta-hydroxy-ageraphorone), as the major constitutive and inducible chemicals ofE. adenophorum. All cadinenes showed potent antifeedant activity against a generalist insectSpodoptera exigua, indicating that they have significant defensive roles.We cloned and functionally characterized a sesquiterpene synthase fromE. adenophorum(EaTPS1), catalyzing the conversion of farnesyl diphosphate to amorpha-4,7(11)-diene and (-)-amorph-4-en-7-ol, which were purified from engineeredEscherichia coliand identified by extensive nuclear magnetic resonance (NMR) spectroscopy.EaTPS1was highly expressed in the aboveground organs, which was congruent with the dominant distribution of cadinenes, suggesting that EaTPS1 is likely involved in cadinene biosynthesis. Mechanical wounding and methyl jasmonate negatively regulatedEaTPS1expression but caused the release of amorpha-4,7(11)-diene and (-)-amorph-4-en-7-ol.Nicotiana benthamianatransiently expressingEaTPS1also produced amorpha-4,7(11)-diene and (-)-amorph-4-en-7-ol, and showed enhanced defense function.The findings presented here uncover the role and formation of the chemical defense mechanism ofE. adenophorum- which probably contributes to the invasive success of this plant - and provide a tool for manipulating the biosynthesis of biologically active cadinene natural products.
Keywordcadinenes defensive chemicals Eupatorium adenophorum sesquiterpenes sesquiterpene synthase NATURAL-PRODUCTS FUNCTIONAL-CHARACTERIZATION ABSOLUTE STEREOCHEMISTRY TERPENE SYNTHASES EXPRESSION CLONING EMISSION LEAVES GENES 9-OXO-10,11-DEHYDROAGERAPHORONE
DOI10.1111/nph.16925
WOS IDWOS:000579700500001
Citation statistics
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/73512
Collection中国科学院昆明植物研究所
Affiliation1.[Liu, Yan
2.Luo, Shi-Hong
3.Hua, Juan
4.Li, De-Sen
5.Ling, Yi
6.Luo, Qian
7.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China
8.Chinese Acad Sci, Kunming Inst Bot, Yunnan Key Lab Nat Med Chem, Kunming 650201, Yunnan, Peoples R China
9.[Luo, Shi-Hong
10.Shenyang Agr Univ, Coll Biosci & Biotechnol, Shenyang 110866, Liaoning, Peoples R China
11.[Li, De-Sen
12.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Liu,Yan,Luo,Shi-Hong,Hua,Juan,et al. Characterization of defensive cadinenes and a novel sesquiterpene synthase responsible for their biosynthesis from the invasive Eupatorium adenophorum[J]. NEW PHYTOLOGIST,2021,229(3):1740-1754.
APA Liu,Yan.,Luo,Shi-Hong.,Hua,Juan.,Li,De-Sen.,Ling,Yi.,...&Li,Sheng-Hong.(2021).Characterization of defensive cadinenes and a novel sesquiterpene synthase responsible for their biosynthesis from the invasive Eupatorium adenophorum.NEW PHYTOLOGIST,229(3),1740-1754.
MLA Liu,Yan,et al."Characterization of defensive cadinenes and a novel sesquiterpene synthase responsible for their biosynthesis from the invasive Eupatorium adenophorum".NEW PHYTOLOGIST 229.3(2021):1740-1754.
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